One-tube fluorogenic reverse transcription-polymerase chain reaction for the quantitation of feline coronaviruses

A one-tube reverse transcription-polymerase chain reaction (RT-PCR) for absolute feline coronavirus (FCoV) quantitation was developed. The assay is based on the 5′ nuclease activity of the Thermus flavus (Tfl) polymerase and a fluorogenic probe which generates fluorescence when it is cleaved. The fl...

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Veröffentlicht in:	Journal of virological methods 1999, Vol.77 (1), p.37-46
Hauptverfasser:	Gut, Marco, M. Leutenegger, Christian, B. Huder, Jon, C. Pedersen, Niels, Lutz, Hans
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Biological and medical sciences Cat Diseases - virology Cats Coronavirus Coronavirus - genetics Coronavirus - isolation & purification Coronavirus Infections - veterinary Coronavirus Infections - virology DNA Primers DNA Probes DNA, Complementary Dogs Exodeoxyribonucleases - metabolism FCoV Feline coronavirus Fluorescent Dyes Fluorogenic 5′ nuclease assay Fundamental and applied biological sciences. Psychology Humans Microbiology Molecular Sequence Data One-tube RT-PCR Quantitation Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Viral - analysis Sensitivity and Specificity Taq Polymerase TaqMan Techniques used in virology Thermus flavus Viral RNA Virology
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