Effect of CD44 on differentiation of human amniotic mesenchymal stem cells into chondrocytes via Smad and ERK signaling pathways

CD44 antigen (CD44) is a transmembrane protein found in cell adhesion molecules and is involved in the regulation of various physiological processes in cells. It was hypothesized that CD44 directly affected the chondrogenic differentiation of human amniotic mesenchymal stem cells (hAMSCs). In the pr...

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Veröffentlicht in:Molecular medicine reports 2020-06, Vol.21 (6), p.2357-2366
Hauptverfasser: Xu, Yan, Wang, Yi-Qing, Wang, Ai-Tong, Yu, Chang-Yin, Luo, Yi, Liu, Ru-Ming, Zhao, Yu-Jie, Xiao, Jian-Hui
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container_issue 6
container_start_page 2357
container_title Molecular medicine reports
container_volume 21
creator Xu, Yan
Wang, Yi-Qing
Wang, Ai-Tong
Yu, Chang-Yin
Luo, Yi
Liu, Ru-Ming
Zhao, Yu-Jie
Xiao, Jian-Hui
description CD44 antigen (CD44) is a transmembrane protein found in cell adhesion molecules and is involved in the regulation of various physiological processes in cells. It was hypothesized that CD44 directly affected the chondrogenic differentiation of human amniotic mesenchymal stem cells (hAMSCs). In the present study, the expression of chondrocyte‑associated factors was detected in the absence and presence of the antibody blocker anti‑CD44 antibody during the chondrogenic differentiation of hAMSCs. Following inhibition of CD44 expression, the transcriptional levels of chondrocyte‑associated genes SRY‑box transcription factor 9, aggrecan and collagen type II α 1 chain, as well as the production of chondrocyte markers type II collagen and aggrecan were significantly decreased in hAMSCs. Further investigation indicated that there was no significant change in total ERK1/2 expression following inhibition of CD44 expression; however, phosphorylated (p)‑ERK1/2 expression was decreased. The expression of p‑Smad2/3 was also upregulated following CD44 inhibition. These data indicated that CD44 may affect the differentiation of hAMSCs into chondrocytes by regulating the Smad2/3 and ERK1/2 signaling pathway.
doi_str_mv 10.3892/mmr.2020.11044
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It was hypothesized that CD44 directly affected the chondrogenic differentiation of human amniotic mesenchymal stem cells (hAMSCs). In the present study, the expression of chondrocyte‑associated factors was detected in the absence and presence of the antibody blocker anti‑CD44 antibody during the chondrogenic differentiation of hAMSCs. Following inhibition of CD44 expression, the transcriptional levels of chondrocyte‑associated genes SRY‑box transcription factor 9, aggrecan and collagen type II α 1 chain, as well as the production of chondrocyte markers type II collagen and aggrecan were significantly decreased in hAMSCs. Further investigation indicated that there was no significant change in total ERK1/2 expression following inhibition of CD44 expression; however, phosphorylated (p)‑ERK1/2 expression was decreased. The expression of p‑Smad2/3 was also upregulated following CD44 inhibition. 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It was hypothesized that CD44 directly affected the chondrogenic differentiation of human amniotic mesenchymal stem cells (hAMSCs). In the present study, the expression of chondrocyte‑associated factors was detected in the absence and presence of the antibody blocker anti‑CD44 antibody during the chondrogenic differentiation of hAMSCs. Following inhibition of CD44 expression, the transcriptional levels of chondrocyte‑associated genes SRY‑box transcription factor 9, aggrecan and collagen type II α 1 chain, as well as the production of chondrocyte markers type II collagen and aggrecan were significantly decreased in hAMSCs. Further investigation indicated that there was no significant change in total ERK1/2 expression following inhibition of CD44 expression; however, phosphorylated (p)‑ERK1/2 expression was decreased. The expression of p‑Smad2/3 was also upregulated following CD44 inhibition. These data indicated that CD44 may affect the differentiation of hAMSCs into chondrocytes by regulating the Smad2/3 and ERK1/2 signaling pathway.</abstract><cop>Greece</cop><pub>Spandidos Publications</pub><pmid>32236637</pmid><doi>10.3892/mmr.2020.11044</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record>
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subjects Aggrecan
Aggrecans - metabolism
Amnion - metabolism
Antibodies
Antigens
Apoptosis
Arthritis
Cartilage
CD44 antigen
Cell adhesion molecules
Cell Differentiation - drug effects
Chondrocytes
Chondrocytes - metabolism
Chondrogenesis
Chondrogenesis - drug effects
Collagen
Collagen (type II)
Collagen Type II - metabolism
Drug dosages
Extracellular signal-regulated kinase
Flow cytometry
Genes
Growth factors
Humans
Hyaluronan Receptors - genetics
Hyaluronan Receptors - metabolism
Kinases
MAP Kinase Signaling System
Mesenchymal stem cells
Mesenchymal Stem Cells - metabolism
Phosphorylation
Proteins
Signal transduction
Signal Transduction - drug effects
Smad protein
Smad2 protein
Smad2 Protein - metabolism
Smad3 Protein - metabolism
Stem cells
Transcription (Genetics)
title Effect of CD44 on differentiation of human amniotic mesenchymal stem cells into chondrocytes via Smad and ERK signaling pathways
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