CXCR4‐SF1 bifunctional adipose‐derived stem cells benefit for the treatment of Leydig cell dysfunction‐related diseases
Stem cell transplantation is a candidate method for the treatment of Leydig cell dysfunction‐related diseases. However, there are still many problems that limit its clinical application. Here, we report the establishment of CXCR4‐SF1 bifunctional adipose‐derived stem cells (CXCR4‐SF1‐ADSCs) and thei...
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| Veröffentlicht in: | Journal of cellular and molecular medicine 2020-04, Vol.24 (8), p.4633-4645 |
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| creator | Li, Xue Xu, Ao Li, Kai Zhang, Jie Li, Qin Zhao, Gang Zhang, Yue Yuan, Hang Guo, Yafei Lin, Ping Huang, Lugang |
| description | Stem cell transplantation is a candidate method for the treatment of Leydig cell dysfunction‐related diseases. However, there are still many problems that limit its clinical application. Here, we report the establishment of CXCR4‐SF1 bifunctional adipose‐derived stem cells (CXCR4‐SF1‐ADSCs) and their reparative effect on Leydig cell dysfunction. CD29+ CD44+ CD34− CD45− ADSCs were isolated from adipose tissue and purified by fluorescence‐activated cell sorting (FACS). Infection with lentiviruses carrying the CXCR4 and SF1 genes was applied to construct CXCR4‐SF1‐ADSCs. The CXCR4‐SF1‐ADSCs exhibited enhanced migration and had the ability to differentiate into Leydig‐like cells in vitro. Furthermore, the bifunctional ADSCs were injected into BPA‐mediated Leydig cell damage model mice via the tail vein. We found that the CXCR4‐SF1‐ADSCs were capable of homing to the injured testes, differentiating into Leydig‐like cells and repairing the deficiency in reproductive function caused by Leydig cell dysfunction. Moreover, we investigated the mechanism underlying SF1‐mediated differentiation and testosterone synthesis in Leydig cells, and the B‐box and SPRY Domain Containing Protein (BSPRY) gene was proposed to be involved in this process. This study provides insight into the treatment of Leydig cell dysfunction‐related diseases. |
| doi_str_mv | 10.1111/jcmm.15128 |
| format | Article |
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However, there are still many problems that limit its clinical application. Here, we report the establishment of CXCR4‐SF1 bifunctional adipose‐derived stem cells (CXCR4‐SF1‐ADSCs) and their reparative effect on Leydig cell dysfunction. CD29+ CD44+ CD34− CD45− ADSCs were isolated from adipose tissue and purified by fluorescence‐activated cell sorting (FACS). Infection with lentiviruses carrying the CXCR4 and SF1 genes was applied to construct CXCR4‐SF1‐ADSCs. The CXCR4‐SF1‐ADSCs exhibited enhanced migration and had the ability to differentiate into Leydig‐like cells in vitro. Furthermore, the bifunctional ADSCs were injected into BPA‐mediated Leydig cell damage model mice via the tail vein. We found that the CXCR4‐SF1‐ADSCs were capable of homing to the injured testes, differentiating into Leydig‐like cells and repairing the deficiency in reproductive function caused by Leydig cell dysfunction. Moreover, we investigated the mechanism underlying SF1‐mediated differentiation and testosterone synthesis in Leydig cells, and the B‐box and SPRY Domain Containing Protein (BSPRY) gene was proposed to be involved in this process. This study provides insight into the treatment of Leydig cell dysfunction‐related diseases.</description><identifier>ISSN: 1582-1838</identifier><identifier>EISSN: 1582-4934</identifier><identifier>DOI: 10.1111/jcmm.15128</identifier><identifier>PMID: 32181567</identifier><language>eng</language><publisher>England: John Wiley and Sons Inc</publisher><subject>Adipocytes - metabolism ; Adipocytes - pathology ; adipose‐derived stem cell ; Animals ; Cell Differentiation - genetics ; Cell Lineage - genetics ; Cells, Cultured ; CXCR4 ; Leydig Cells - metabolism ; Leydig Cells - pathology ; Leydig Cells - transplantation ; Male ; Mesenchymal Stem Cells - metabolism ; Mice ; Original ; Proteins - genetics ; Receptors, CXCR4 - genetics ; SF1 ; Signal Transduction - genetics ; Stem Cell Transplantation ; Steroidogenic Factor 1 - genetics ; Testis - metabolism ; Testis - pathology ; transplantation</subject><ispartof>Journal of cellular and molecular medicine, 2020-04, Vol.24 (8), p.4633-4645</ispartof><rights>2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4208-f6a64528536583719e61b4767841b3eed07aa4a4fa9efc5d519e9ffb13ea596c3</citedby><cites>FETCH-LOGICAL-c4208-f6a64528536583719e61b4767841b3eed07aa4a4fa9efc5d519e9ffb13ea596c3</cites><orcidid>0000-0001-9750-7956</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7176872/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7176872/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,1417,11562,27924,27925,45574,45575,46052,46476,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32181567$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Xue</creatorcontrib><creatorcontrib>Xu, Ao</creatorcontrib><creatorcontrib>Li, Kai</creatorcontrib><creatorcontrib>Zhang, Jie</creatorcontrib><creatorcontrib>Li, Qin</creatorcontrib><creatorcontrib>Zhao, Gang</creatorcontrib><creatorcontrib>Zhang, Yue</creatorcontrib><creatorcontrib>Yuan, Hang</creatorcontrib><creatorcontrib>Guo, Yafei</creatorcontrib><creatorcontrib>Lin, Ping</creatorcontrib><creatorcontrib>Huang, Lugang</creatorcontrib><title>CXCR4‐SF1 bifunctional adipose‐derived stem cells benefit for the treatment of Leydig cell dysfunction‐related diseases</title><title>Journal of cellular and molecular medicine</title><addtitle>J Cell Mol Med</addtitle><description>Stem cell transplantation is a candidate method for the treatment of Leydig cell dysfunction‐related diseases. However, there are still many problems that limit its clinical application. Here, we report the establishment of CXCR4‐SF1 bifunctional adipose‐derived stem cells (CXCR4‐SF1‐ADSCs) and their reparative effect on Leydig cell dysfunction. CD29+ CD44+ CD34− CD45− ADSCs were isolated from adipose tissue and purified by fluorescence‐activated cell sorting (FACS). Infection with lentiviruses carrying the CXCR4 and SF1 genes was applied to construct CXCR4‐SF1‐ADSCs. The CXCR4‐SF1‐ADSCs exhibited enhanced migration and had the ability to differentiate into Leydig‐like cells in vitro. Furthermore, the bifunctional ADSCs were injected into BPA‐mediated Leydig cell damage model mice via the tail vein. We found that the CXCR4‐SF1‐ADSCs were capable of homing to the injured testes, differentiating into Leydig‐like cells and repairing the deficiency in reproductive function caused by Leydig cell dysfunction. Moreover, we investigated the mechanism underlying SF1‐mediated differentiation and testosterone synthesis in Leydig cells, and the B‐box and SPRY Domain Containing Protein (BSPRY) gene was proposed to be involved in this process. This study provides insight into the treatment of Leydig cell dysfunction‐related diseases.</description><subject>Adipocytes - metabolism</subject><subject>Adipocytes - pathology</subject><subject>adipose‐derived stem cell</subject><subject>Animals</subject><subject>Cell Differentiation - genetics</subject><subject>Cell Lineage - genetics</subject><subject>Cells, Cultured</subject><subject>CXCR4</subject><subject>Leydig Cells - metabolism</subject><subject>Leydig Cells - pathology</subject><subject>Leydig Cells - transplantation</subject><subject>Male</subject><subject>Mesenchymal Stem Cells - metabolism</subject><subject>Mice</subject><subject>Original</subject><subject>Proteins - genetics</subject><subject>Receptors, CXCR4 - genetics</subject><subject>SF1</subject><subject>Signal Transduction - genetics</subject><subject>Stem Cell Transplantation</subject><subject>Steroidogenic Factor 1 - genetics</subject><subject>Testis - metabolism</subject><subject>Testis - pathology</subject><subject>transplantation</subject><issn>1582-1838</issn><issn>1582-4934</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><recordid>eNp9kd1qFDEUx4Mo_bI3PoDkUoStk8nn3AgytFXZUmgVvAuZyUmbMjNZk2xlLwp9BJ_RJzHb3ZZ6Y25O4Pz4nXP4I_SGVEekvA83_TgeEU5q9QLtEa7qGWsoe7n9E0XVLtpP6aaqqCC02UG7tCaKcCH30F37o71gf-5_X54Q3Hm3nPrsw2QGbKxfhASlZSH6W7A4ZRhxD8OQcAcTOJ-xCxHna8A5gskjTBkHh-ewsv7qgcR2lR6dxRRhMLmYrE9gEqTX6JUzQ4LDbT1A30-Ov7WfZ_Pz0y_tp_msZ3WlZk4YwXitOBVcUUkaEKRjUkjFSEcBbCWNYYY504DrueWFaJzrCAXDG9HTA_Rx410suxFsXxaNZtCL6EcTVzoYr__tTP5aX4VbLYkUStZF8G4riOHnElLWo0_rA80EYZl0TaVslFKcF_T9Bu1jSCmCexpDKr3OS6_z0g95Ffjt88We0MeACkA2wC8_wOo_Kv21PTvbSP8Cuy-mRQ</recordid><startdate>202004</startdate><enddate>202004</enddate><creator>Li, Xue</creator><creator>Xu, Ao</creator><creator>Li, Kai</creator><creator>Zhang, Jie</creator><creator>Li, Qin</creator><creator>Zhao, Gang</creator><creator>Zhang, Yue</creator><creator>Yuan, Hang</creator><creator>Guo, Yafei</creator><creator>Lin, Ping</creator><creator>Huang, Lugang</creator><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-9750-7956</orcidid></search><sort><creationdate>202004</creationdate><title>CXCR4‐SF1 bifunctional adipose‐derived stem cells benefit for the treatment of Leydig cell dysfunction‐related diseases</title><author>Li, Xue ; Xu, Ao ; Li, Kai ; Zhang, Jie ; Li, Qin ; Zhao, Gang ; Zhang, Yue ; Yuan, Hang ; Guo, Yafei ; Lin, Ping ; Huang, Lugang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4208-f6a64528536583719e61b4767841b3eed07aa4a4fa9efc5d519e9ffb13ea596c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adipocytes - metabolism</topic><topic>Adipocytes - pathology</topic><topic>adipose‐derived stem cell</topic><topic>Animals</topic><topic>Cell Differentiation - genetics</topic><topic>Cell Lineage - genetics</topic><topic>Cells, Cultured</topic><topic>CXCR4</topic><topic>Leydig Cells - metabolism</topic><topic>Leydig Cells - pathology</topic><topic>Leydig Cells - transplantation</topic><topic>Male</topic><topic>Mesenchymal Stem Cells - metabolism</topic><topic>Mice</topic><topic>Original</topic><topic>Proteins - genetics</topic><topic>Receptors, CXCR4 - genetics</topic><topic>SF1</topic><topic>Signal Transduction - genetics</topic><topic>Stem Cell Transplantation</topic><topic>Steroidogenic Factor 1 - genetics</topic><topic>Testis - metabolism</topic><topic>Testis - pathology</topic><topic>transplantation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Xue</creatorcontrib><creatorcontrib>Xu, Ao</creatorcontrib><creatorcontrib>Li, Kai</creatorcontrib><creatorcontrib>Zhang, Jie</creatorcontrib><creatorcontrib>Li, Qin</creatorcontrib><creatorcontrib>Zhao, Gang</creatorcontrib><creatorcontrib>Zhang, Yue</creatorcontrib><creatorcontrib>Yuan, Hang</creatorcontrib><creatorcontrib>Guo, Yafei</creatorcontrib><creatorcontrib>Lin, Ping</creatorcontrib><creatorcontrib>Huang, Lugang</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>Wiley Online Library (Open Access Collection)</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of cellular and molecular medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Xue</au><au>Xu, Ao</au><au>Li, Kai</au><au>Zhang, Jie</au><au>Li, Qin</au><au>Zhao, Gang</au><au>Zhang, Yue</au><au>Yuan, Hang</au><au>Guo, Yafei</au><au>Lin, Ping</au><au>Huang, Lugang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CXCR4‐SF1 bifunctional adipose‐derived stem cells benefit for the treatment of Leydig cell dysfunction‐related diseases</atitle><jtitle>Journal of cellular and molecular medicine</jtitle><addtitle>J Cell Mol Med</addtitle><date>2020-04</date><risdate>2020</risdate><volume>24</volume><issue>8</issue><spage>4633</spage><epage>4645</epage><pages>4633-4645</pages><issn>1582-1838</issn><eissn>1582-4934</eissn><abstract>Stem cell transplantation is a candidate method for the treatment of Leydig cell dysfunction‐related diseases. However, there are still many problems that limit its clinical application. Here, we report the establishment of CXCR4‐SF1 bifunctional adipose‐derived stem cells (CXCR4‐SF1‐ADSCs) and their reparative effect on Leydig cell dysfunction. CD29+ CD44+ CD34− CD45− ADSCs were isolated from adipose tissue and purified by fluorescence‐activated cell sorting (FACS). Infection with lentiviruses carrying the CXCR4 and SF1 genes was applied to construct CXCR4‐SF1‐ADSCs. The CXCR4‐SF1‐ADSCs exhibited enhanced migration and had the ability to differentiate into Leydig‐like cells in vitro. Furthermore, the bifunctional ADSCs were injected into BPA‐mediated Leydig cell damage model mice via the tail vein. We found that the CXCR4‐SF1‐ADSCs were capable of homing to the injured testes, differentiating into Leydig‐like cells and repairing the deficiency in reproductive function caused by Leydig cell dysfunction. Moreover, we investigated the mechanism underlying SF1‐mediated differentiation and testosterone synthesis in Leydig cells, and the B‐box and SPRY Domain Containing Protein (BSPRY) gene was proposed to be involved in this process. This study provides insight into the treatment of Leydig cell dysfunction‐related diseases.</abstract><cop>England</cop><pub>John Wiley and Sons Inc</pub><pmid>32181567</pmid><doi>10.1111/jcmm.15128</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0001-9750-7956</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adipocytes - metabolism Adipocytes - pathology adipose‐derived stem cell Animals Cell Differentiation - genetics Cell Lineage - genetics Cells, Cultured CXCR4 Leydig Cells - metabolism Leydig Cells - pathology Leydig Cells - transplantation Male Mesenchymal Stem Cells - metabolism Mice Original Proteins - genetics Receptors, CXCR4 - genetics SF1 Signal Transduction - genetics Stem Cell Transplantation Steroidogenic Factor 1 - genetics Testis - metabolism Testis - pathology transplantation |
| title | CXCR4‐SF1 bifunctional adipose‐derived stem cells benefit for the treatment of Leydig cell dysfunction‐related diseases |
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