MIF inhibitor, ISO-1, attenuates human pancreatic cancer cell proliferation, migration and invasion in vitro, and suppresses xenograft tumour growth in vivo
This study sought to investigate the biological effects of specific MIF inhibitor, ISO-1, on the proliferation, migration and invasion of PANC-1 human pancreatic cells in vitro , and on tumour growth in a xenograft tumour model in vivo . The effect of ISO-1 on PANC-1 cell proliferation was examined...
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| Veröffentlicht in: | Scientific reports 2020-04, Vol.10 (1), p.6741-6741, Article 6741 |
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| creator | Cheng, Bo Wang, Qiaofang Song, Yaodong Liu, Yanna Liu, Yanyan Yang, Shujun Li, Dejian Zhang, Yan Zhu, Changju |
| description | This study sought to investigate the biological effects of specific MIF inhibitor, ISO-1, on the proliferation, migration and invasion of PANC-1 human pancreatic cells
in vitro
, and on tumour growth in a xenograft tumour model
in vivo
. The effect of ISO-1 on PANC-1 cell proliferation was examined using CCK-8 cell proliferation assay. The effect of ISO-1 on collective cell migration and recolonization of PANC-1 cells was evaluated using the cell-wound closure migration assay. The effect of ISO-1 on the migration and invasion of individual PANC-1 cells in a 3-dimensional environment in response to a chemo-attractant was investigated through the use of Transwell migration/invasion assays. Quantitative real time PCR and western blot analyses were employed to investigate the effects of ISO-1 on MIF, NF-κB p65 and TNF-α mRNA and protein expression respectively. Finally, a xenograft tumor model in BALB/c nude mice were used to assess the
in vivo
effects of ISO-1 on PANC-1-induced tumor growth. We found high expression of MIF in pancreatic cancer tissues. We demonstrated that ISO-1 exerts anti-cancer effects on PANC-1 cell proliferation, migration and invasion
in vitro
, and inhibited PANC-1 cell-induced tumour growth in xenograft mice
in vivo
. Our data suggests that ISO-1 and its derivative may have potential therapeutic applications in pancreatic cancer. |
| doi_str_mv | 10.1038/s41598-020-63778-y |
| format | Article |
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in vitro
, and on tumour growth in a xenograft tumour model
in vivo
. The effect of ISO-1 on PANC-1 cell proliferation was examined using CCK-8 cell proliferation assay. The effect of ISO-1 on collective cell migration and recolonization of PANC-1 cells was evaluated using the cell-wound closure migration assay. The effect of ISO-1 on the migration and invasion of individual PANC-1 cells in a 3-dimensional environment in response to a chemo-attractant was investigated through the use of Transwell migration/invasion assays. Quantitative real time PCR and western blot analyses were employed to investigate the effects of ISO-1 on MIF, NF-κB p65 and TNF-α mRNA and protein expression respectively. Finally, a xenograft tumor model in BALB/c nude mice were used to assess the
in vivo
effects of ISO-1 on PANC-1-induced tumor growth. We found high expression of MIF in pancreatic cancer tissues. We demonstrated that ISO-1 exerts anti-cancer effects on PANC-1 cell proliferation, migration and invasion
in vitro
, and inhibited PANC-1 cell-induced tumour growth in xenograft mice
in vivo
. Our data suggests that ISO-1 and its derivative may have potential therapeutic applications in pancreatic cancer.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-020-63778-y</identifier><identifier>PMID: 32317702</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/154 ; 631/67 ; Adenocarcinoma - drug therapy ; Adenocarcinoma - genetics ; Adenocarcinoma - metabolism ; Adenocarcinoma - pathology ; Animals ; Antineoplastic Agents - pharmacology ; Biological Assay ; Biological effects ; Cell adhesion & migration ; Cell growth ; Cell Line, Tumor ; Cell migration ; Cell Movement - drug effects ; Cell proliferation ; Cell Proliferation - drug effects ; Cholecystokinin ; Diffusion Chambers, Culture ; Enzyme Inhibitors - pharmacology ; Female ; Gene expression ; Gene Expression Regulation, Neoplastic ; Humanities and Social Sciences ; Humans ; Intramolecular Oxidoreductases - genetics ; Intramolecular Oxidoreductases - metabolism ; Isoxazoles - pharmacology ; Kinases ; Macrophage Migration-Inhibitory Factors - genetics ; Macrophage Migration-Inhibitory Factors - metabolism ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; mRNA ; multidisciplinary ; Neoplasm Invasiveness ; NF-κB protein ; Pancreatic cancer ; Pancreatic Neoplasms - drug therapy ; Pancreatic Neoplasms - genetics ; Pancreatic Neoplasms - metabolism ; Pancreatic Neoplasms - pathology ; Recolonization ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Science ; Science (multidisciplinary) ; Signal Transduction ; Therapeutic applications ; Transcription Factor RelA - genetics ; Transcription Factor RelA - metabolism ; Tumor Burden - drug effects ; Tumor Necrosis Factor-alpha - genetics ; Tumor Necrosis Factor-alpha - metabolism ; Tumor necrosis factor-α ; Tumors ; Xenograft Model Antitumor Assays ; Xenografts</subject><ispartof>Scientific reports, 2020-04, Vol.10 (1), p.6741-6741, Article 6741</ispartof><rights>The Author(s) 2020</rights><rights>The Author(s) 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-dd2d35fc1f65f02614ebd11485eb61c53e9ee3ae5f8b3b61f72943ed6b1e3ea73</citedby><cites>FETCH-LOGICAL-c474t-dd2d35fc1f65f02614ebd11485eb61c53e9ee3ae5f8b3b61f72943ed6b1e3ea73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7174354/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7174354/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32317702$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cheng, Bo</creatorcontrib><creatorcontrib>Wang, Qiaofang</creatorcontrib><creatorcontrib>Song, Yaodong</creatorcontrib><creatorcontrib>Liu, Yanna</creatorcontrib><creatorcontrib>Liu, Yanyan</creatorcontrib><creatorcontrib>Yang, Shujun</creatorcontrib><creatorcontrib>Li, Dejian</creatorcontrib><creatorcontrib>Zhang, Yan</creatorcontrib><creatorcontrib>Zhu, Changju</creatorcontrib><title>MIF inhibitor, ISO-1, attenuates human pancreatic cancer cell proliferation, migration and invasion in vitro, and suppresses xenograft tumour growth in vivo</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>This study sought to investigate the biological effects of specific MIF inhibitor, ISO-1, on the proliferation, migration and invasion of PANC-1 human pancreatic cells
in vitro
, and on tumour growth in a xenograft tumour model
in vivo
. The effect of ISO-1 on PANC-1 cell proliferation was examined using CCK-8 cell proliferation assay. The effect of ISO-1 on collective cell migration and recolonization of PANC-1 cells was evaluated using the cell-wound closure migration assay. The effect of ISO-1 on the migration and invasion of individual PANC-1 cells in a 3-dimensional environment in response to a chemo-attractant was investigated through the use of Transwell migration/invasion assays. Quantitative real time PCR and western blot analyses were employed to investigate the effects of ISO-1 on MIF, NF-κB p65 and TNF-α mRNA and protein expression respectively. Finally, a xenograft tumor model in BALB/c nude mice were used to assess the
in vivo
effects of ISO-1 on PANC-1-induced tumor growth. We found high expression of MIF in pancreatic cancer tissues. We demonstrated that ISO-1 exerts anti-cancer effects on PANC-1 cell proliferation, migration and invasion
in vitro
, and inhibited PANC-1 cell-induced tumour growth in xenograft mice
in vivo
. Our data suggests that ISO-1 and its derivative may have potential therapeutic applications in pancreatic cancer.</description><subject>631/154</subject><subject>631/67</subject><subject>Adenocarcinoma - drug therapy</subject><subject>Adenocarcinoma - genetics</subject><subject>Adenocarcinoma - metabolism</subject><subject>Adenocarcinoma - pathology</subject><subject>Animals</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Biological Assay</subject><subject>Biological effects</subject><subject>Cell adhesion & migration</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Cell migration</subject><subject>Cell Movement - drug effects</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - drug effects</subject><subject>Cholecystokinin</subject><subject>Diffusion Chambers, Culture</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Intramolecular Oxidoreductases - genetics</subject><subject>Intramolecular Oxidoreductases - metabolism</subject><subject>Isoxazoles - pharmacology</subject><subject>Kinases</subject><subject>Macrophage Migration-Inhibitory Factors - genetics</subject><subject>Macrophage Migration-Inhibitory Factors - metabolism</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Nude</subject><subject>mRNA</subject><subject>multidisciplinary</subject><subject>Neoplasm Invasiveness</subject><subject>NF-κB protein</subject><subject>Pancreatic cancer</subject><subject>Pancreatic Neoplasms - drug therapy</subject><subject>Pancreatic Neoplasms - genetics</subject><subject>Pancreatic Neoplasms - metabolism</subject><subject>Pancreatic Neoplasms - pathology</subject><subject>Recolonization</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Signal Transduction</subject><subject>Therapeutic applications</subject><subject>Transcription Factor RelA - genetics</subject><subject>Transcription Factor RelA - metabolism</subject><subject>Tumor Burden - drug effects</subject><subject>Tumor Necrosis Factor-alpha - genetics</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><subject>Tumor necrosis factor-α</subject><subject>Tumors</subject><subject>Xenograft Model Antitumor Assays</subject><subject>Xenografts</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9Uctu1DAUtRCIVqU_wAJZYsNiAn7EcbJBQhUtIxV1AawtJ7mecZXYwXYG5l_42HompRQWeOP7OOfY9x6EXlLylhJev4slFU1dEEaKiktZF_sn6JSRUhSMM_b0UXyCzmO8JfkI1pS0eY5OOONUSsJO0a_P60ts3da2NvmwwusvNwVdYZ0SuFkniHg7j9rhSbsugE62w10OIeAOhgFPwQ_WQMgN71Z4tJslxNr1WXan4yGxDu9sCn51LMd5mgLEmLV_gvOZYRJO8-jngDfB_0jbhbDzL9Azo4cI5_f3Gfp2-fHrxafi-uZqffHhuuhKWaai71nPhemoqYQhrKIltD2lZS2grWgnODQAXIMwdctzxci8Bw591VLgoCU_Q-8X3WluR-g7cCnoQU3BjjrslddW_d1xdqs2fqcklSUXZRZ4cy8Q_PcZYlKjjYcFaQd-jorxhouaMsky9PU_0Ns8uMvjHVGESs4PKLaguuBjDGAePkOJOvivFv9V9l8d_Vf7THr1eIwHym-3M4AvgJhbbgPhz9v_kb0DFcy_0w</recordid><startdate>20200421</startdate><enddate>20200421</enddate><creator>Cheng, Bo</creator><creator>Wang, Qiaofang</creator><creator>Song, Yaodong</creator><creator>Liu, Yanna</creator><creator>Liu, Yanyan</creator><creator>Yang, Shujun</creator><creator>Li, Dejian</creator><creator>Zhang, Yan</creator><creator>Zhu, Changju</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20200421</creationdate><title>MIF inhibitor, ISO-1, attenuates human pancreatic cancer cell proliferation, migration and invasion in vitro, and suppresses xenograft tumour growth in vivo</title><author>Cheng, Bo ; Wang, Qiaofang ; Song, Yaodong ; Liu, Yanna ; Liu, Yanyan ; Yang, Shujun ; Li, Dejian ; Zhang, Yan ; Zhu, Changju</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-dd2d35fc1f65f02614ebd11485eb61c53e9ee3ae5f8b3b61f72943ed6b1e3ea73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>631/154</topic><topic>631/67</topic><topic>Adenocarcinoma - drug therapy</topic><topic>Adenocarcinoma - genetics</topic><topic>Adenocarcinoma - metabolism</topic><topic>Adenocarcinoma - pathology</topic><topic>Animals</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Biological Assay</topic><topic>Biological effects</topic><topic>Cell adhesion & migration</topic><topic>Cell growth</topic><topic>Cell Line, Tumor</topic><topic>Cell migration</topic><topic>Cell Movement - drug effects</topic><topic>Cell proliferation</topic><topic>Cell Proliferation - drug effects</topic><topic>Cholecystokinin</topic><topic>Diffusion Chambers, Culture</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Intramolecular Oxidoreductases - genetics</topic><topic>Intramolecular Oxidoreductases - metabolism</topic><topic>Isoxazoles - pharmacology</topic><topic>Kinases</topic><topic>Macrophage Migration-Inhibitory Factors - genetics</topic><topic>Macrophage Migration-Inhibitory Factors - metabolism</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Nude</topic><topic>mRNA</topic><topic>multidisciplinary</topic><topic>Neoplasm Invasiveness</topic><topic>NF-κB protein</topic><topic>Pancreatic cancer</topic><topic>Pancreatic Neoplasms - drug therapy</topic><topic>Pancreatic Neoplasms - genetics</topic><topic>Pancreatic Neoplasms - metabolism</topic><topic>Pancreatic Neoplasms - pathology</topic><topic>Recolonization</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Signal Transduction</topic><topic>Therapeutic applications</topic><topic>Transcription Factor RelA - genetics</topic><topic>Transcription Factor RelA - metabolism</topic><topic>Tumor Burden - drug effects</topic><topic>Tumor Necrosis Factor-alpha - genetics</topic><topic>Tumor Necrosis Factor-alpha - metabolism</topic><topic>Tumor necrosis factor-α</topic><topic>Tumors</topic><topic>Xenograft Model Antitumor Assays</topic><topic>Xenografts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cheng, Bo</creatorcontrib><creatorcontrib>Wang, Qiaofang</creatorcontrib><creatorcontrib>Song, Yaodong</creatorcontrib><creatorcontrib>Liu, Yanna</creatorcontrib><creatorcontrib>Liu, Yanyan</creatorcontrib><creatorcontrib>Yang, Shujun</creatorcontrib><creatorcontrib>Li, Dejian</creatorcontrib><creatorcontrib>Zhang, Yan</creatorcontrib><creatorcontrib>Zhu, Changju</creatorcontrib><collection>SpringerOpen (Open Access)</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Science Database (ProQuest)</collection><collection>ProQuest Biological Science Journals</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cheng, Bo</au><au>Wang, Qiaofang</au><au>Song, Yaodong</au><au>Liu, Yanna</au><au>Liu, Yanyan</au><au>Yang, Shujun</au><au>Li, Dejian</au><au>Zhang, Yan</au><au>Zhu, Changju</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MIF inhibitor, ISO-1, attenuates human pancreatic cancer cell proliferation, migration and invasion in vitro, and suppresses xenograft tumour growth in vivo</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2020-04-21</date><risdate>2020</risdate><volume>10</volume><issue>1</issue><spage>6741</spage><epage>6741</epage><pages>6741-6741</pages><artnum>6741</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>This study sought to investigate the biological effects of specific MIF inhibitor, ISO-1, on the proliferation, migration and invasion of PANC-1 human pancreatic cells
in vitro
, and on tumour growth in a xenograft tumour model
in vivo
. The effect of ISO-1 on PANC-1 cell proliferation was examined using CCK-8 cell proliferation assay. The effect of ISO-1 on collective cell migration and recolonization of PANC-1 cells was evaluated using the cell-wound closure migration assay. The effect of ISO-1 on the migration and invasion of individual PANC-1 cells in a 3-dimensional environment in response to a chemo-attractant was investigated through the use of Transwell migration/invasion assays. Quantitative real time PCR and western blot analyses were employed to investigate the effects of ISO-1 on MIF, NF-κB p65 and TNF-α mRNA and protein expression respectively. Finally, a xenograft tumor model in BALB/c nude mice were used to assess the
in vivo
effects of ISO-1 on PANC-1-induced tumor growth. We found high expression of MIF in pancreatic cancer tissues. We demonstrated that ISO-1 exerts anti-cancer effects on PANC-1 cell proliferation, migration and invasion
in vitro
, and inhibited PANC-1 cell-induced tumour growth in xenograft mice
in vivo
. Our data suggests that ISO-1 and its derivative may have potential therapeutic applications in pancreatic cancer.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>32317702</pmid><doi>10.1038/s41598-020-63778-y</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| source | Electronic Journals Library; PubMed Central (Open Access); MEDLINE; DOAJ Directory of Open Access Journals; SpringerOpen (Open Access); Nature Journals Online; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | 631/154 631/67 Adenocarcinoma - drug therapy Adenocarcinoma - genetics Adenocarcinoma - metabolism Adenocarcinoma - pathology Animals Antineoplastic Agents - pharmacology Biological Assay Biological effects Cell adhesion & migration Cell growth Cell Line, Tumor Cell migration Cell Movement - drug effects Cell proliferation Cell Proliferation - drug effects Cholecystokinin Diffusion Chambers, Culture Enzyme Inhibitors - pharmacology Female Gene expression Gene Expression Regulation, Neoplastic Humanities and Social Sciences Humans Intramolecular Oxidoreductases - genetics Intramolecular Oxidoreductases - metabolism Isoxazoles - pharmacology Kinases Macrophage Migration-Inhibitory Factors - genetics Macrophage Migration-Inhibitory Factors - metabolism Male Mice Mice, Inbred BALB C Mice, Nude mRNA multidisciplinary Neoplasm Invasiveness NF-κB protein Pancreatic cancer Pancreatic Neoplasms - drug therapy Pancreatic Neoplasms - genetics Pancreatic Neoplasms - metabolism Pancreatic Neoplasms - pathology Recolonization RNA, Messenger - genetics RNA, Messenger - metabolism Science Science (multidisciplinary) Signal Transduction Therapeutic applications Transcription Factor RelA - genetics Transcription Factor RelA - metabolism Tumor Burden - drug effects Tumor Necrosis Factor-alpha - genetics Tumor Necrosis Factor-alpha - metabolism Tumor necrosis factor-α Tumors Xenograft Model Antitumor Assays Xenografts |
| title | MIF inhibitor, ISO-1, attenuates human pancreatic cancer cell proliferation, migration and invasion in vitro, and suppresses xenograft tumour growth in vivo |
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