The use of red cells with fused Semliki Forest virus envelope proteins in antibody determinations by hemolysis in gel

Chicken red blood cells with fused Semliki Forest virus (SFV) proteins on the cell membrane were used in the hemolysis-in-gel (HIG) plates. Optimally the plate contained a 1.5 mm thick gel of 1% agarose with 1% red cells and 1 unit/ml gel of complement. 400 ng of SFV was fused to the red cells in 1...

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Veröffentlicht in:	Journal of virological methods 1982-03, Vol.4 (2), p.117-126
1. Verfasser:	Vaananen, Pertti
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Viral - analysis Antibodies, Viral - immunology Antibody Specificity Chickens - blood Erythrocytes - immunology hemolysis in gel Hemolytic Plaque Technique In Vitro Techniques Semliki Forest virus Semliki forest virus - immunology Viral Proteins - immunology
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