Imaging-assisted hydrogel formation for single cell isolation
We report a flexible single-cell isolation method by imaging-assisted hydrogel formation. Our approach consists of imaging-aided selective capture of cells of interest by encasing them into a polymeric hydrogel, followed by removal of unwanted cells and subsequent release of isolated cells by enzyma...
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| Veröffentlicht in: | Scientific reports 2020-04, Vol.10 (1), p.6595-6595, Article 6595 |
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| creator | Oldenhof, Sander Mytnyk, Serhii Arranja, Alexandra de Puit, Marcel van Esch, Jan H. |
| description | We report a flexible single-cell isolation method by imaging-assisted hydrogel formation. Our approach consists of imaging-aided selective capture of cells of interest by encasing them into a polymeric hydrogel, followed by removal of unwanted cells and subsequent release of isolated cells by enzymatic hydrogel degradation, thus offering an opportunity for further analysis or cultivation of selected cells. We achieved high sorting efficiency and observed excellent viability rates (>98%) for NIH/3T3 fibroblasts and A549 carcinoma cells isolated using this procedure. The method presented here offers a mask-free, cost-efficient and easy-to-use alternative to many currently existing surface-based cell-sorting techniques, and has the potential to impact the field of cell culturing and isolation, e.g. single cell genomics and proteomics, investigation of cellular heterogeneity and isolation of best performing mutants for developing new cell lines. |
| doi_str_mv | 10.1038/s41598-020-62623-6 |
| format | Article |
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Our approach consists of imaging-aided selective capture of cells of interest by encasing them into a polymeric hydrogel, followed by removal of unwanted cells and subsequent release of isolated cells by enzymatic hydrogel degradation, thus offering an opportunity for further analysis or cultivation of selected cells. We achieved high sorting efficiency and observed excellent viability rates (>98%) for NIH/3T3 fibroblasts and A549 carcinoma cells isolated using this procedure. The method presented here offers a mask-free, cost-efficient and easy-to-use alternative to many currently existing surface-based cell-sorting techniques, and has the potential to impact the field of cell culturing and isolation, e.g. single cell genomics and proteomics, investigation of cellular heterogeneity and isolation of best performing mutants for developing new cell lines.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-020-62623-6</identifier><identifier>PMID: 32313146</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/80 ; 639/638/298/923/1027 ; A549 Cells ; Animals ; Cell Separation - methods ; Cell Survival ; Dextrans - chemistry ; Fibroblasts ; Heterogeneity ; Humanities and Social Sciences ; Humans ; Hydrogels ; Hydrogels - chemistry ; Imaging, Three-Dimensional ; Methacrylates - chemistry ; Mice ; Microscopy, Confocal ; multidisciplinary ; NIH 3T3 Cells ; Proteomics ; Science ; Science (multidisciplinary) ; Single-Cell Analysis</subject><ispartof>Scientific reports, 2020-04, Vol.10 (1), p.6595-6595, Article 6595</ispartof><rights>The Author(s) 2020</rights><rights>The Author(s) 2020. 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| subjects | 631/80 639/638/298/923/1027 A549 Cells Animals Cell Separation - methods Cell Survival Dextrans - chemistry Fibroblasts Heterogeneity Humanities and Social Sciences Humans Hydrogels Hydrogels - chemistry Imaging, Three-Dimensional Methacrylates - chemistry Mice Microscopy, Confocal multidisciplinary NIH 3T3 Cells Proteomics Science Science (multidisciplinary) Single-Cell Analysis |
| title | Imaging-assisted hydrogel formation for single cell isolation |
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