The RpoN2‐PilRX regulatory system governs type IV pilus gene transcription and is required for bacterial motility and virulence in Xanthomonas oryzae pv. oryzae
The type IV pilus (T4P), a special class of bacterial surface filament, plays crucial roles in surface adhesion, motility, biofilm formation, and virulence in pathogenic bacteria. However, the regulatory mechanism of T4P and its relationship to bacterial virulence are still little understood in Xant...
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| Veröffentlicht in: | Molecular plant pathology 2020-05, Vol.21 (5), p.652-666 |
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| creator | Yu, Chao Nguyen, Doan-Phuong Ren, Zhaoyu Liu, Jianan Yang, Fenghuan Tian, Fang Fan, Susu Chen, Huamin |
| description | The type IV pilus (T4P), a special class of bacterial surface filament, plays crucial roles in surface adhesion, motility, biofilm formation, and virulence in pathogenic bacteria. However, the regulatory mechanism of T4P and its relationship to bacterial virulence are still little understood in Xanthomonas oryzae pv. oryzae (Xoo), the causal pathogen of bacterial blight of rice. Our previous studies showed that the σ54 factor RpoN2 regulated bacterial virulence on rice in a flagellum‐independent manner in Xoo. In this study, both yeast two‐hybrid and pull‐down assays revealed that RpoN2 directly and specifically interacted with PilRX, a homolog of the response regulator PilR of the two‐component system PilS‐PilR in the pilus gene cluster. Genomic sequence and reverse transcription PCR (RT‐PCR) analysis showed 13 regulons containing 25 genes encoding T4P structural components and putative regulators. A consensus RpoN2‐binding sequence GGN10GC was identified in the promoter sequences of most T4P gene transcriptional units. Electrophoretic mobility shift assays confirmed the direct binding of RpoN2 to the promoter of the major pilin gene pilAX, the inner membrane platform protein gene pilCX, and pilRX. Promoter activity and quantitative RT‐PCR assays demonstrated direct and indirect transcriptional regulation by RpoN2 of the T4P genes. In addition, individual deletions of pilAX, pilCX, and pilRX resulted in significantly reduced twitching and swimming motility, biofilm formation, and virulence in rice. Taken together, the findings from the current study suggest that the RpoN2‐PilRX regulatory system controls bacterial motility and virulence by regulating T4P gene transcription in Xoo.
The σ54 factor RpoN2 directly interacts with PilRX, and controls bacterial motility and virulence by regulating type IV pilus gene transcription in Xanthomonas oryzae pv. oryzae. |
| doi_str_mv | 10.1111/mpp.12920 |
| format | Article |
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The σ54 factor RpoN2 directly interacts with PilRX, and controls bacterial motility and virulence by regulating type IV pilus gene transcription in Xanthomonas oryzae pv. oryzae.</description><identifier>ISSN: 1464-6722</identifier><identifier>EISSN: 1364-3703</identifier><identifier>DOI: 10.1111/mpp.12920</identifier><identifier>PMID: 32112711</identifier><language>eng</language><publisher>England: John Wiley & Sons, Inc</publisher><subject>Assaying ; Bacteria ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Binding ; Biofilms ; Biosynthesis ; Blight ; Cell adhesion & migration ; Conserved sequence ; Deoxyribonucleic acid ; DNA ; Electrophoretic mobility ; Electrophoretic Mobility Shift Assay ; Flagella ; Gene expression ; Gene Expression Regulation, Bacterial - genetics ; Gene Expression Regulation, Bacterial - physiology ; Gene regulation ; Genes ; Genetic aspects ; Genetic research ; Genetic transcription ; Homology ; Kinases ; Membrane proteins ; Motility ; Original ; Oryza - microbiology ; Pilin ; Pilin gene ; PilRX ; Plasmids ; Promoter Regions, Genetic - genetics ; Promoters (Genetics) ; Proteins ; Regulatory mechanisms (biology) ; Reverse transcription ; Rice ; RNA polymerase ; Swimming ; Twitching ; Two-Hybrid System Techniques ; type IV pilus ; Virulence ; Virulence (Microbiology) ; Virulence - genetics ; Virulence - physiology ; Xanthomonas - pathogenicity ; Xanthomonas oryzae ; Xanthomonas oryzae pv. oryzae ; Yeasts ; σ54</subject><ispartof>Molecular plant pathology, 2020-05, Vol.21 (5), p.652-666</ispartof><rights>2020 The Authors. published by British Society for Plant Pathology and John Wiley & Sons Ltd</rights><rights>2020 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.</rights><rights>COPYRIGHT 2020 John Wiley & Sons, Inc.</rights><rights>2020. This work is published under http://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4820-9dcacda0a6079eacd631b132b0251ade4780dfb190f5767f486cd5282e9f54b03</citedby><cites>FETCH-LOGICAL-c4820-9dcacda0a6079eacd631b132b0251ade4780dfb190f5767f486cd5282e9f54b03</cites><orcidid>0000-0001-7409-8328 ; 0000-0003-3645-9895</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7170775/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7170775/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,1417,11562,27924,27925,45574,45575,46052,46476,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32112711$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yu, Chao</creatorcontrib><creatorcontrib>Nguyen, Doan-Phuong</creatorcontrib><creatorcontrib>Ren, Zhaoyu</creatorcontrib><creatorcontrib>Liu, Jianan</creatorcontrib><creatorcontrib>Yang, Fenghuan</creatorcontrib><creatorcontrib>Tian, Fang</creatorcontrib><creatorcontrib>Fan, Susu</creatorcontrib><creatorcontrib>Chen, Huamin</creatorcontrib><title>The RpoN2‐PilRX regulatory system governs type IV pilus gene transcription and is required for bacterial motility and virulence in Xanthomonas oryzae pv. oryzae</title><title>Molecular plant pathology</title><addtitle>Mol Plant Pathol</addtitle><description>The type IV pilus (T4P), a special class of bacterial surface filament, plays crucial roles in surface adhesion, motility, biofilm formation, and virulence in pathogenic bacteria. However, the regulatory mechanism of T4P and its relationship to bacterial virulence are still little understood in Xanthomonas oryzae pv. oryzae (Xoo), the causal pathogen of bacterial blight of rice. Our previous studies showed that the σ54 factor RpoN2 regulated bacterial virulence on rice in a flagellum‐independent manner in Xoo. In this study, both yeast two‐hybrid and pull‐down assays revealed that RpoN2 directly and specifically interacted with PilRX, a homolog of the response regulator PilR of the two‐component system PilS‐PilR in the pilus gene cluster. Genomic sequence and reverse transcription PCR (RT‐PCR) analysis showed 13 regulons containing 25 genes encoding T4P structural components and putative regulators. A consensus RpoN2‐binding sequence GGN10GC was identified in the promoter sequences of most T4P gene transcriptional units. Electrophoretic mobility shift assays confirmed the direct binding of RpoN2 to the promoter of the major pilin gene pilAX, the inner membrane platform protein gene pilCX, and pilRX. Promoter activity and quantitative RT‐PCR assays demonstrated direct and indirect transcriptional regulation by RpoN2 of the T4P genes. In addition, individual deletions of pilAX, pilCX, and pilRX resulted in significantly reduced twitching and swimming motility, biofilm formation, and virulence in rice. Taken together, the findings from the current study suggest that the RpoN2‐PilRX regulatory system controls bacterial motility and virulence by regulating T4P gene transcription in Xoo.
The σ54 factor RpoN2 directly interacts with PilRX, and controls bacterial motility and virulence by regulating type IV pilus gene transcription in Xanthomonas oryzae pv. oryzae.</description><subject>Assaying</subject><subject>Bacteria</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Binding</subject><subject>Biofilms</subject><subject>Biosynthesis</subject><subject>Blight</subject><subject>Cell adhesion & migration</subject><subject>Conserved sequence</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Electrophoretic mobility</subject><subject>Electrophoretic Mobility Shift Assay</subject><subject>Flagella</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Bacterial - genetics</subject><subject>Gene Expression Regulation, Bacterial - physiology</subject><subject>Gene regulation</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genetic research</subject><subject>Genetic transcription</subject><subject>Homology</subject><subject>Kinases</subject><subject>Membrane proteins</subject><subject>Motility</subject><subject>Original</subject><subject>Oryza - microbiology</subject><subject>Pilin</subject><subject>Pilin gene</subject><subject>PilRX</subject><subject>Plasmids</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Promoters (Genetics)</subject><subject>Proteins</subject><subject>Regulatory mechanisms (biology)</subject><subject>Reverse transcription</subject><subject>Rice</subject><subject>RNA polymerase</subject><subject>Swimming</subject><subject>Twitching</subject><subject>Two-Hybrid System Techniques</subject><subject>type IV pilus</subject><subject>Virulence</subject><subject>Virulence (Microbiology)</subject><subject>Virulence - genetics</subject><subject>Virulence - physiology</subject><subject>Xanthomonas - pathogenicity</subject><subject>Xanthomonas oryzae</subject><subject>Xanthomonas oryzae pv. oryzae</subject><subject>Yeasts</subject><subject>σ54</subject><issn>1464-6722</issn><issn>1364-3703</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1Uctu1DAUjRCIlsKCH0CWWLGYqe08nGyQqopHpUJHVUHdWY5zM-MqsVPbGRRWfALf0E_rl3DbTCtY4I2P7HPPPfeeJHnN6JLhOeyHYcl4xemTZJ-lRbZIBU2fIs4QF4LzveRFCFeUMlHx_Hmyl3LGuGBsP7m52AA5H9xXfvvr98p055fEw3rsVHR-ImEKEXqydlvwNpA4DUBOvpPBdGMga7BAolc2aG-GaJwlyjbEBFS4Ho2HhrTOk1rpCN6ojvQums7E6Z62NX7swGogxpJLZePG9c6qQLDvTwVk2C538GXyrFVdgFe7-yD59vHDxfHnxenZp5Pjo9OFzkpOF1WjlW4UVQUVFSAsUlazlNeU50w1kImSNm3NKtrmohBtVha6yXnJoWrzrKbpQfJ-1h3GuodGg8XhOjl40ys_SaeM_PfHmo3E1UjBBBUiR4G3OwHvrkcIUV650Vv0LHla4cpLzhmyljNrrTqQxrYOxdA6WuyNdhZag-9HAjNKeV7eFbybC7R3IXhoHy0xKu_yl5i_vM8fuW_-nuGR-RA4Eg5nwg_sMv1fSX5ZrWbJP0LQvyQ</recordid><startdate>202005</startdate><enddate>202005</enddate><creator>Yu, Chao</creator><creator>Nguyen, Doan-Phuong</creator><creator>Ren, Zhaoyu</creator><creator>Liu, Jianan</creator><creator>Yang, Fenghuan</creator><creator>Tian, Fang</creator><creator>Fan, Susu</creator><creator>Chen, Huamin</creator><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7U9</scope><scope>7X2</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-7409-8328</orcidid><orcidid>https://orcid.org/0000-0003-3645-9895</orcidid></search><sort><creationdate>202005</creationdate><title>The RpoN2‐PilRX regulatory system governs type IV pilus gene transcription and is required for bacterial motility and virulence in Xanthomonas oryzae pv. oryzae</title><author>Yu, Chao ; Nguyen, Doan-Phuong ; Ren, Zhaoyu ; Liu, Jianan ; Yang, Fenghuan ; Tian, Fang ; Fan, Susu ; Chen, Huamin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4820-9dcacda0a6079eacd631b132b0251ade4780dfb190f5767f486cd5282e9f54b03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Assaying</topic><topic>Bacteria</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Binding</topic><topic>Biofilms</topic><topic>Biosynthesis</topic><topic>Blight</topic><topic>Cell adhesion & migration</topic><topic>Conserved sequence</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Electrophoretic mobility</topic><topic>Electrophoretic Mobility Shift Assay</topic><topic>Flagella</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Bacterial - genetics</topic><topic>Gene Expression Regulation, Bacterial - physiology</topic><topic>Gene regulation</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Genetic research</topic><topic>Genetic transcription</topic><topic>Homology</topic><topic>Kinases</topic><topic>Membrane proteins</topic><topic>Motility</topic><topic>Original</topic><topic>Oryza - microbiology</topic><topic>Pilin</topic><topic>Pilin gene</topic><topic>PilRX</topic><topic>Plasmids</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Promoters (Genetics)</topic><topic>Proteins</topic><topic>Regulatory mechanisms (biology)</topic><topic>Reverse transcription</topic><topic>Rice</topic><topic>RNA polymerase</topic><topic>Swimming</topic><topic>Twitching</topic><topic>Two-Hybrid System Techniques</topic><topic>type IV pilus</topic><topic>Virulence</topic><topic>Virulence (Microbiology)</topic><topic>Virulence - genetics</topic><topic>Virulence - physiology</topic><topic>Xanthomonas - pathogenicity</topic><topic>Xanthomonas oryzae</topic><topic>Xanthomonas oryzae pv. oryzae</topic><topic>Yeasts</topic><topic>σ54</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yu, Chao</creatorcontrib><creatorcontrib>Nguyen, Doan-Phuong</creatorcontrib><creatorcontrib>Ren, Zhaoyu</creatorcontrib><creatorcontrib>Liu, Jianan</creatorcontrib><creatorcontrib>Yang, Fenghuan</creatorcontrib><creatorcontrib>Tian, Fang</creatorcontrib><creatorcontrib>Fan, Susu</creatorcontrib><creatorcontrib>Chen, Huamin</creatorcontrib><collection>Wiley Online Library (Open Access Collection)</collection><collection>Wiley Online Library (Open Access Collection)</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular plant pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yu, Chao</au><au>Nguyen, Doan-Phuong</au><au>Ren, Zhaoyu</au><au>Liu, Jianan</au><au>Yang, Fenghuan</au><au>Tian, Fang</au><au>Fan, Susu</au><au>Chen, Huamin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The RpoN2‐PilRX regulatory system governs type IV pilus gene transcription and is required for bacterial motility and virulence in Xanthomonas oryzae pv. oryzae</atitle><jtitle>Molecular plant pathology</jtitle><addtitle>Mol Plant Pathol</addtitle><date>2020-05</date><risdate>2020</risdate><volume>21</volume><issue>5</issue><spage>652</spage><epage>666</epage><pages>652-666</pages><issn>1464-6722</issn><eissn>1364-3703</eissn><abstract>The type IV pilus (T4P), a special class of bacterial surface filament, plays crucial roles in surface adhesion, motility, biofilm formation, and virulence in pathogenic bacteria. However, the regulatory mechanism of T4P and its relationship to bacterial virulence are still little understood in Xanthomonas oryzae pv. oryzae (Xoo), the causal pathogen of bacterial blight of rice. Our previous studies showed that the σ54 factor RpoN2 regulated bacterial virulence on rice in a flagellum‐independent manner in Xoo. In this study, both yeast two‐hybrid and pull‐down assays revealed that RpoN2 directly and specifically interacted with PilRX, a homolog of the response regulator PilR of the two‐component system PilS‐PilR in the pilus gene cluster. Genomic sequence and reverse transcription PCR (RT‐PCR) analysis showed 13 regulons containing 25 genes encoding T4P structural components and putative regulators. A consensus RpoN2‐binding sequence GGN10GC was identified in the promoter sequences of most T4P gene transcriptional units. Electrophoretic mobility shift assays confirmed the direct binding of RpoN2 to the promoter of the major pilin gene pilAX, the inner membrane platform protein gene pilCX, and pilRX. Promoter activity and quantitative RT‐PCR assays demonstrated direct and indirect transcriptional regulation by RpoN2 of the T4P genes. In addition, individual deletions of pilAX, pilCX, and pilRX resulted in significantly reduced twitching and swimming motility, biofilm formation, and virulence in rice. Taken together, the findings from the current study suggest that the RpoN2‐PilRX regulatory system controls bacterial motility and virulence by regulating T4P gene transcription in Xoo.
The σ54 factor RpoN2 directly interacts with PilRX, and controls bacterial motility and virulence by regulating type IV pilus gene transcription in Xanthomonas oryzae pv. oryzae.</abstract><cop>England</cop><pub>John Wiley & Sons, Inc</pub><pmid>32112711</pmid><doi>10.1111/mpp.12920</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0001-7409-8328</orcidid><orcidid>https://orcid.org/0000-0003-3645-9895</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Molecular plant pathology, 2020-05, Vol.21 (5), p.652-666 |
| issn | 1464-6722 1364-3703 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7170775 |
| source | MEDLINE; Wiley Journals; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; Wiley Online Library (Open Access Collection); PubMed Central |
| subjects | Assaying Bacteria Bacterial Proteins - genetics Bacterial Proteins - metabolism Binding Biofilms Biosynthesis Blight Cell adhesion & migration Conserved sequence Deoxyribonucleic acid DNA Electrophoretic mobility Electrophoretic Mobility Shift Assay Flagella Gene expression Gene Expression Regulation, Bacterial - genetics Gene Expression Regulation, Bacterial - physiology Gene regulation Genes Genetic aspects Genetic research Genetic transcription Homology Kinases Membrane proteins Motility Original Oryza - microbiology Pilin Pilin gene PilRX Plasmids Promoter Regions, Genetic - genetics Promoters (Genetics) Proteins Regulatory mechanisms (biology) Reverse transcription Rice RNA polymerase Swimming Twitching Two-Hybrid System Techniques type IV pilus Virulence Virulence (Microbiology) Virulence - genetics Virulence - physiology Xanthomonas - pathogenicity Xanthomonas oryzae Xanthomonas oryzae pv. oryzae Yeasts σ54 |
| title | The RpoN2‐PilRX regulatory system governs type IV pilus gene transcription and is required for bacterial motility and virulence in Xanthomonas oryzae pv. oryzae |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T12%3A07%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20RpoN2%E2%80%90PilRX%20regulatory%20system%20governs%20type%20IV%20pilus%20gene%20transcription%20and%20is%20required%20for%20bacterial%20motility%20and%20virulence%20in%20Xanthomonas%20oryzae%20pv.%20oryzae&rft.jtitle=Molecular%20plant%20pathology&rft.au=Yu,%20Chao&rft.date=2020-05&rft.volume=21&rft.issue=5&rft.spage=652&rft.epage=666&rft.pages=652-666&rft.issn=1464-6722&rft.eissn=1364-3703&rft_id=info:doi/10.1111/mpp.12920&rft_dat=%3Cgale_pubme%3EA732132581%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2392118221&rft_id=info:pmid/32112711&rft_galeid=A732132581&rfr_iscdi=true |