The microbial abundance dynamics of the paediatric oral cavity before and after sleep
Objective: Microhabitats in the oral cavity differ in microbial taxonomy. However, abundance variations of bacterial and viral communities within these microhabitats are not fully understood. Aims and Hypothesis: To assess the spatial distribution and dynamics of the microbial abundances within 6 mi...
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| Veröffentlicht in: | Journal of oral microbiology 2020-01, Vol.12 (1), p.1741254-1741254 |
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| container_title | Journal of oral microbiology |
| container_volume | 12 |
| creator | Carlson-Jones, Jessica A. P. Kontos, Anna Kennedy, Declan Martin, James Lushington, Kurt McKerral, Jody Paterson, James S. Smith, Renee J. Dann, Lisa M. Speck, Peter Mitchell, James G. |
| description | Objective: Microhabitats in the oral cavity differ in microbial taxonomy. However, abundance variations of bacterial and viral communities within these microhabitats are not fully understood.
Aims and Hypothesis: To assess the spatial distribution and dynamics of the microbial abundances within 6 microhabitats of the oral cavity before and after sleep. We hypothesise that the abundance distributions of these microbial communities will differ among oral sites.
Methods: Using flow cytometry, bacterial and virus-like particle (VLP) abundances were enumerated for 6 oral microhabitats before and after sleep in 10 healthy paediatric sleepers.
Results: Bacterial counts ranged from 7.2 ± 2.8 × 10
5
at the palate before sleep to 1.3 ± 0.2 × 10
8
at the back of the tongue after sleep, a difference of 187 times. VLPs ranged from 1.9 ± 1.0 × 10
6
at the palate before sleep to 9.2 ± 5.0 × 10
7
at the back of the tongue after sleep, a difference of 48 times.
Conclusion: The oral cavity is a dynamic numerically heterogeneous environment where microbial communities can increase by a count of 100 million during sleep. Quantification of the paediatric oral microbiome complements taxonomic diversity information to show how biomass varies and shifts in space and time. |
| doi_str_mv | 10.1080/20002297.2020.1741254 |
| format | Article |
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Aims and Hypothesis: To assess the spatial distribution and dynamics of the microbial abundances within 6 microhabitats of the oral cavity before and after sleep. We hypothesise that the abundance distributions of these microbial communities will differ among oral sites.
Methods: Using flow cytometry, bacterial and virus-like particle (VLP) abundances were enumerated for 6 oral microhabitats before and after sleep in 10 healthy paediatric sleepers.
Results: Bacterial counts ranged from 7.2 ± 2.8 × 10
5
at the palate before sleep to 1.3 ± 0.2 × 10
8
at the back of the tongue after sleep, a difference of 187 times. VLPs ranged from 1.9 ± 1.0 × 10
6
at the palate before sleep to 9.2 ± 5.0 × 10
7
at the back of the tongue after sleep, a difference of 48 times.
Conclusion: The oral cavity is a dynamic numerically heterogeneous environment where microbial communities can increase by a count of 100 million during sleep. Quantification of the paediatric oral microbiome complements taxonomic diversity information to show how biomass varies and shifts in space and time.</description><identifier>ISSN: 2000-2297</identifier><identifier>EISSN: 2000-2297</identifier><identifier>DOI: 10.1080/20002297.2020.1741254</identifier><identifier>PMID: 32341758</identifier><language>eng</language><publisher>United States: Taylor & Francis</publisher><subject>bacteria ; flow cytometry ; Oral microbiome ; Original ; sleep ; viruses</subject><ispartof>Journal of oral microbiology, 2020-01, Vol.12 (1), p.1741254-1741254</ispartof><rights>2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. 2020</rights><rights>2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.</rights><rights>2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. 2020 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c534t-6ee330c1bd741f73405860cd904458b5eb1fbdba4e72fcb9b8c8917b961e5b193</citedby><cites>FETCH-LOGICAL-c534t-6ee330c1bd741f73405860cd904458b5eb1fbdba4e72fcb9b8c8917b961e5b193</cites><orcidid>0000-0002-2862-0338</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7170375/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7170375/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,27502,27924,27925,53791,53793,59143,59144</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32341758$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Carlson-Jones, Jessica A. P.</creatorcontrib><creatorcontrib>Kontos, Anna</creatorcontrib><creatorcontrib>Kennedy, Declan</creatorcontrib><creatorcontrib>Martin, James</creatorcontrib><creatorcontrib>Lushington, Kurt</creatorcontrib><creatorcontrib>McKerral, Jody</creatorcontrib><creatorcontrib>Paterson, James S.</creatorcontrib><creatorcontrib>Smith, Renee J.</creatorcontrib><creatorcontrib>Dann, Lisa M.</creatorcontrib><creatorcontrib>Speck, Peter</creatorcontrib><creatorcontrib>Mitchell, James G.</creatorcontrib><title>The microbial abundance dynamics of the paediatric oral cavity before and after sleep</title><title>Journal of oral microbiology</title><addtitle>J Oral Microbiol</addtitle><description>Objective: Microhabitats in the oral cavity differ in microbial taxonomy. However, abundance variations of bacterial and viral communities within these microhabitats are not fully understood.
Aims and Hypothesis: To assess the spatial distribution and dynamics of the microbial abundances within 6 microhabitats of the oral cavity before and after sleep. We hypothesise that the abundance distributions of these microbial communities will differ among oral sites.
Methods: Using flow cytometry, bacterial and virus-like particle (VLP) abundances were enumerated for 6 oral microhabitats before and after sleep in 10 healthy paediatric sleepers.
Results: Bacterial counts ranged from 7.2 ± 2.8 × 10
5
at the palate before sleep to 1.3 ± 0.2 × 10
8
at the back of the tongue after sleep, a difference of 187 times. VLPs ranged from 1.9 ± 1.0 × 10
6
at the palate before sleep to 9.2 ± 5.0 × 10
7
at the back of the tongue after sleep, a difference of 48 times.
Conclusion: The oral cavity is a dynamic numerically heterogeneous environment where microbial communities can increase by a count of 100 million during sleep. Quantification of the paediatric oral microbiome complements taxonomic diversity information to show how biomass varies and shifts in space and time.</description><subject>bacteria</subject><subject>flow cytometry</subject><subject>Oral microbiome</subject><subject>Original</subject><subject>sleep</subject><subject>viruses</subject><issn>2000-2297</issn><issn>2000-2297</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><sourceid>DOA</sourceid><recordid>eNp9kU1PHDEMhkdVUUGUnwDKsZeFfG4ml6oV6gcSUi9wjpzEgaCZyZLMUu2_b7a7ILhwSmK_fh376bpTRs8Z7ekFp5RybvQ5p7yFtGRcyQ_d0Ta-2CY-vrofdie1PrQXFbzvJf_UHQouJNOqP-pub-6RjMmX7BIMBNx6CjB5JGEzQYtXkiOZm2YFGBLMJXmSS1N6eErzhjiMuSCBKRCIMxZSB8TV5-4gwlDxZH8ed7c_f9xc_l5c__l1dfn9euGVkPNiiSgE9cyFNkHUQlLVL6kPhkqpeqfQseiCA4maR--M631vmHZmyVA5ZsRxd7XzDRke7KqkEcrGZkj2fyCXOwtlTn5Aq4LmykkTGbYdaGU0D-CFcRrRB--a19ed12rtRgwep7nN-cb0bWZK9_YuP1nNNBVaNYMve4OSH9dYZzum6nEYYMK8rpYLo5ZUUiObVO2kbe-1FowvbRi1W8L2mbDdErZ7wq3u7PUfX6qeeTbBt50gTY3LCH9zGYKdYTPkEksDm6oV7_f4B0kHteU</recordid><startdate>20200101</startdate><enddate>20200101</enddate><creator>Carlson-Jones, Jessica A. P.</creator><creator>Kontos, Anna</creator><creator>Kennedy, Declan</creator><creator>Martin, James</creator><creator>Lushington, Kurt</creator><creator>McKerral, Jody</creator><creator>Paterson, James S.</creator><creator>Smith, Renee J.</creator><creator>Dann, Lisa M.</creator><creator>Speck, Peter</creator><creator>Mitchell, James G.</creator><general>Taylor & Francis</general><general>Taylor & Francis Group</general><scope>0YH</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-2862-0338</orcidid></search><sort><creationdate>20200101</creationdate><title>The microbial abundance dynamics of the paediatric oral cavity before and after sleep</title><author>Carlson-Jones, Jessica A. P. ; Kontos, Anna ; Kennedy, Declan ; Martin, James ; Lushington, Kurt ; McKerral, Jody ; Paterson, James S. ; Smith, Renee J. ; Dann, Lisa M. ; Speck, Peter ; Mitchell, James G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c534t-6ee330c1bd741f73405860cd904458b5eb1fbdba4e72fcb9b8c8917b961e5b193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>bacteria</topic><topic>flow cytometry</topic><topic>Oral microbiome</topic><topic>Original</topic><topic>sleep</topic><topic>viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carlson-Jones, Jessica A. P.</creatorcontrib><creatorcontrib>Kontos, Anna</creatorcontrib><creatorcontrib>Kennedy, Declan</creatorcontrib><creatorcontrib>Martin, James</creatorcontrib><creatorcontrib>Lushington, Kurt</creatorcontrib><creatorcontrib>McKerral, Jody</creatorcontrib><creatorcontrib>Paterson, James S.</creatorcontrib><creatorcontrib>Smith, Renee J.</creatorcontrib><creatorcontrib>Dann, Lisa M.</creatorcontrib><creatorcontrib>Speck, Peter</creatorcontrib><creatorcontrib>Mitchell, James G.</creatorcontrib><collection>Taylor & Francis Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Journal of oral microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carlson-Jones, Jessica A. P.</au><au>Kontos, Anna</au><au>Kennedy, Declan</au><au>Martin, James</au><au>Lushington, Kurt</au><au>McKerral, Jody</au><au>Paterson, James S.</au><au>Smith, Renee J.</au><au>Dann, Lisa M.</au><au>Speck, Peter</au><au>Mitchell, James G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The microbial abundance dynamics of the paediatric oral cavity before and after sleep</atitle><jtitle>Journal of oral microbiology</jtitle><addtitle>J Oral Microbiol</addtitle><date>2020-01-01</date><risdate>2020</risdate><volume>12</volume><issue>1</issue><spage>1741254</spage><epage>1741254</epage><pages>1741254-1741254</pages><issn>2000-2297</issn><eissn>2000-2297</eissn><abstract>Objective: Microhabitats in the oral cavity differ in microbial taxonomy. However, abundance variations of bacterial and viral communities within these microhabitats are not fully understood.
Aims and Hypothesis: To assess the spatial distribution and dynamics of the microbial abundances within 6 microhabitats of the oral cavity before and after sleep. We hypothesise that the abundance distributions of these microbial communities will differ among oral sites.
Methods: Using flow cytometry, bacterial and virus-like particle (VLP) abundances were enumerated for 6 oral microhabitats before and after sleep in 10 healthy paediatric sleepers.
Results: Bacterial counts ranged from 7.2 ± 2.8 × 10
5
at the palate before sleep to 1.3 ± 0.2 × 10
8
at the back of the tongue after sleep, a difference of 187 times. VLPs ranged from 1.9 ± 1.0 × 10
6
at the palate before sleep to 9.2 ± 5.0 × 10
7
at the back of the tongue after sleep, a difference of 48 times.
Conclusion: The oral cavity is a dynamic numerically heterogeneous environment where microbial communities can increase by a count of 100 million during sleep. Quantification of the paediatric oral microbiome complements taxonomic diversity information to show how biomass varies and shifts in space and time.</abstract><cop>United States</cop><pub>Taylor & Francis</pub><pmid>32341758</pmid><doi>10.1080/20002297.2020.1741254</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-2862-0338</orcidid><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | Taylor & Francis Open Access; DOAJ Directory of Open Access Journals; Co-Action Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | bacteria flow cytometry Oral microbiome Original sleep viruses |
| title | The microbial abundance dynamics of the paediatric oral cavity before and after sleep |
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