Validation and application of normalization factors for gene expression studies in rubella virus-infected cell lines with quantitative real-time PCR

Reference genes are generally employed in real‐time quantitative PCR (RT‐qPCR) experiments to normalize variability between different samples. The aim of this study was to identify and validate appropriate reference genes as internal controls for RT‐qPCR experiments in rubella virus (RV)‐infected Ve...

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Veröffentlicht in:	Journal of cellular biochemistry 2010-05, Vol.110 (1), p.118-128
Hauptverfasser:	Chey, S., Claus, C., Liebert, U.G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Line, Tumor Chlorocebus aethiops COS Cells gene expression Gene Expression Profiling - methods Gene Expression Profiling - standards Gene Expression Regulation Gene Expression Regulation, Neoplastic Genome, Viral - genetics Humans normalization factors Protein Binding quantitative real-time PCR reference genes Reference Standards Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction - methods Reverse Transcriptase Polymerase Chain Reaction - standards RNA, Messenger - genetics RNA, Messenger - metabolism Rubella - genetics Rubella virus Rubella virus - genetics Rubella virus - physiology Software Species Specificity
Online-Zugang:	Volltext
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