Efficient evaluation of a gene containment system for poplar through early flowering induction
Key message The early flowering system HSP:: AtFT allowed a fast evaluation of a gene containment system based on the construct PsEND1:: barnase–barstar for poplar . Transgenic lines showed disturbed pollen development and sterility. Vertical gene transfer through pollen flow from transgenic or non-...
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| Veröffentlicht in: | Plant cell reports 2020-05, Vol.39 (5), p.577-587 |
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| creator | Briones, M. Valentina Hoenicka, Hans Cañas, Luis A. Beltrán, José Pío Hanelt, Dieter Sharry, Sandra Fladung, Matthias |
| description | Key message
The early flowering system HSP::
AtFT
allowed a fast evaluation of a gene containment system based on the construct PsEND1::
barnase–barstar
for poplar
.
Transgenic lines showed disturbed pollen development and sterility.
Vertical gene transfer through pollen flow from transgenic or non-native plant species into their crossable natural relatives is a major concern. Gene containment approaches have been proposed to reduce or even avoid gene flow among tree species. However, evaluation of genetic containment strategies for trees is very difficult due to the long-generation times. Early flowering induction would allow faster evaluation of genetic containment in this case. Although no reliable methods were available for the induction of fertile flowers in poplar, recently, a new early flowering approach was developed. In this study, early flowering poplar lines containing the gene construct PsEND1::
barnase–barstar
were obtained. The PsEND1 promoter was chosen due to its early expression pattern, its versality and efficiency for generation of male-sterile plants fused to the
barnase
gene. RT-PCRs confirmed
barnase
gene activity in flowers, and pollen development was disturbed, leading to sterile flowers. The system developed in this study represents a valuable tool for gene containment studies in forest tree species. |
| doi_str_mv | 10.1007/s00299-020-02515-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7165154</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2391145693</sourcerecordid><originalsourceid>FETCH-LOGICAL-c474t-10229790b363800bedf417be8ba3e9e3eb3628b4b5b46f3d108af641e562a1ae3</originalsourceid><addsrcrecordid>eNp9kU1v1DAQhi0EokvhD3BAlrhwCczYTpxckFBVPqRKXEDihOVkx7uuEjvYSdH-e7xsKR8HDtYc3mdez8zL2FOElwigX2UA0XUVCCivxrrCe2yDSopKgPxyn21AC6y0RnXGHuV8DVBE3TxkZ1JALVDoDft66ZwfPIWF040dV7v4GHh03PIdBeJDDIv1YToC-ZAXmriLic9xHm3iyz7FdbfnZNN44G6M3yn5sOM-bNfh6PSYPXB2zPTktp6zz28vP128r64-vvtw8eaqGpRWS4UgRKc76GUjW4Cetk6h7qntraSOJBVBtL3q6141Tm4RWusahVQ3wqIlec5en3zntZ9oO5Rxkx3NnPxk08FE683fSvB7s4s3RmNTLqeKwYtbgxS_rZQXM_k80DjaQHHNRshaadmgaAv6_B_0Oq4plPUK1SGquulkocSJGlLMOZG7GwbBHOMzp_hMic_8jM9gaXr25xp3Lb_yKoA8AXk-HprS77__Y_sD5rOnSg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2391145693</pqid></control><display><type>article</type><title>Efficient evaluation of a gene containment system for poplar through early flowering induction</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Briones, M. Valentina ; Hoenicka, Hans ; Cañas, Luis A. ; Beltrán, José Pío ; Hanelt, Dieter ; Sharry, Sandra ; Fladung, Matthias</creator><creatorcontrib>Briones, M. Valentina ; Hoenicka, Hans ; Cañas, Luis A. ; Beltrán, José Pío ; Hanelt, Dieter ; Sharry, Sandra ; Fladung, Matthias</creatorcontrib><description>Key message
The early flowering system HSP::
AtFT
allowed a fast evaluation of a gene containment system based on the construct PsEND1::
barnase–barstar
for poplar
.
Transgenic lines showed disturbed pollen development and sterility.
Vertical gene transfer through pollen flow from transgenic or non-native plant species into their crossable natural relatives is a major concern. Gene containment approaches have been proposed to reduce or even avoid gene flow among tree species. However, evaluation of genetic containment strategies for trees is very difficult due to the long-generation times. Early flowering induction would allow faster evaluation of genetic containment in this case. Although no reliable methods were available for the induction of fertile flowers in poplar, recently, a new early flowering approach was developed. In this study, early flowering poplar lines containing the gene construct PsEND1::
barnase–barstar
were obtained. The PsEND1 promoter was chosen due to its early expression pattern, its versality and efficiency for generation of male-sterile plants fused to the
barnase
gene. RT-PCRs confirmed
barnase
gene activity in flowers, and pollen development was disturbed, leading to sterile flowers. The system developed in this study represents a valuable tool for gene containment studies in forest tree species.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s00299-020-02515-1</identifier><identifier>PMID: 32052127</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Arabidopsis Proteins - genetics ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Barnase ; Barstar ; Biomedical and Life Sciences ; Biotechnology ; Cell Biology ; Containment ; Flowering ; Flowers ; Flowers - genetics ; Flowers - growth & development ; Flowers - metabolism ; Flowers - radiation effects ; Gene Editing - methods ; Gene Expression Regulation, Plant ; Gene Flow ; Gene transfer ; Genetic Vectors ; Heat-Shock Response ; Indigenous plants ; Indigenous species ; Introduced species ; Life Sciences ; Original ; Original Article ; Plant Biochemistry ; Plant Infertility - genetics ; Plant Sciences ; Plant species ; Plants, Genetically Modified - growth & development ; Plants, Genetically Modified - metabolism ; Plants, Genetically Modified - radiation effects ; Pollen ; Pollen - genetics ; Pollen - growth & development ; Poplar ; Populus - genetics ; Populus - growth & development ; Populus - metabolism ; Populus - radiation effects ; Promoter Regions, Genetic ; Ribonucleases - genetics ; Ribonucleases - metabolism ; Sterility ; Temperature ; Transformation, Genetic ; Transgenic plants</subject><ispartof>Plant cell reports, 2020-05, Vol.39 (5), p.577-587</ispartof><rights>The Author(s) 2020</rights><rights>The Author(s) 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-10229790b363800bedf417be8ba3e9e3eb3628b4b5b46f3d108af641e562a1ae3</citedby><cites>FETCH-LOGICAL-c474t-10229790b363800bedf417be8ba3e9e3eb3628b4b5b46f3d108af641e562a1ae3</cites><orcidid>0000-0001-9301-8581</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00299-020-02515-1$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00299-020-02515-1$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32052127$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Briones, M. Valentina</creatorcontrib><creatorcontrib>Hoenicka, Hans</creatorcontrib><creatorcontrib>Cañas, Luis A.</creatorcontrib><creatorcontrib>Beltrán, José Pío</creatorcontrib><creatorcontrib>Hanelt, Dieter</creatorcontrib><creatorcontrib>Sharry, Sandra</creatorcontrib><creatorcontrib>Fladung, Matthias</creatorcontrib><title>Efficient evaluation of a gene containment system for poplar through early flowering induction</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><addtitle>Plant Cell Rep</addtitle><description>Key message
The early flowering system HSP::
AtFT
allowed a fast evaluation of a gene containment system based on the construct PsEND1::
barnase–barstar
for poplar
.
Transgenic lines showed disturbed pollen development and sterility.
Vertical gene transfer through pollen flow from transgenic or non-native plant species into their crossable natural relatives is a major concern. Gene containment approaches have been proposed to reduce or even avoid gene flow among tree species. However, evaluation of genetic containment strategies for trees is very difficult due to the long-generation times. Early flowering induction would allow faster evaluation of genetic containment in this case. Although no reliable methods were available for the induction of fertile flowers in poplar, recently, a new early flowering approach was developed. In this study, early flowering poplar lines containing the gene construct PsEND1::
barnase–barstar
were obtained. The PsEND1 promoter was chosen due to its early expression pattern, its versality and efficiency for generation of male-sterile plants fused to the
barnase
gene. RT-PCRs confirmed
barnase
gene activity in flowers, and pollen development was disturbed, leading to sterile flowers. The system developed in this study represents a valuable tool for gene containment studies in forest tree species.</description><subject>Arabidopsis Proteins - genetics</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Barnase</subject><subject>Barstar</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cell Biology</subject><subject>Containment</subject><subject>Flowering</subject><subject>Flowers</subject><subject>Flowers - genetics</subject><subject>Flowers - growth & development</subject><subject>Flowers - metabolism</subject><subject>Flowers - radiation effects</subject><subject>Gene Editing - methods</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene Flow</subject><subject>Gene transfer</subject><subject>Genetic Vectors</subject><subject>Heat-Shock Response</subject><subject>Indigenous plants</subject><subject>Indigenous species</subject><subject>Introduced species</subject><subject>Life Sciences</subject><subject>Original</subject><subject>Original Article</subject><subject>Plant Biochemistry</subject><subject>Plant Infertility - genetics</subject><subject>Plant Sciences</subject><subject>Plant species</subject><subject>Plants, Genetically Modified - growth & development</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>Plants, Genetically Modified - radiation effects</subject><subject>Pollen</subject><subject>Pollen - genetics</subject><subject>Pollen - growth & development</subject><subject>Poplar</subject><subject>Populus - genetics</subject><subject>Populus - growth & development</subject><subject>Populus - metabolism</subject><subject>Populus - radiation effects</subject><subject>Promoter Regions, Genetic</subject><subject>Ribonucleases - genetics</subject><subject>Ribonucleases - metabolism</subject><subject>Sterility</subject><subject>Temperature</subject><subject>Transformation, Genetic</subject><subject>Transgenic plants</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU1v1DAQhi0EokvhD3BAlrhwCczYTpxckFBVPqRKXEDihOVkx7uuEjvYSdH-e7xsKR8HDtYc3mdez8zL2FOElwigX2UA0XUVCCivxrrCe2yDSopKgPxyn21AC6y0RnXGHuV8DVBE3TxkZ1JALVDoDft66ZwfPIWF040dV7v4GHh03PIdBeJDDIv1YToC-ZAXmriLic9xHm3iyz7FdbfnZNN44G6M3yn5sOM-bNfh6PSYPXB2zPTktp6zz28vP128r64-vvtw8eaqGpRWS4UgRKc76GUjW4Cetk6h7qntraSOJBVBtL3q6141Tm4RWusahVQ3wqIlec5en3zntZ9oO5Rxkx3NnPxk08FE683fSvB7s4s3RmNTLqeKwYtbgxS_rZQXM_k80DjaQHHNRshaadmgaAv6_B_0Oq4plPUK1SGquulkocSJGlLMOZG7GwbBHOMzp_hMic_8jM9gaXr25xp3Lb_yKoA8AXk-HprS77__Y_sD5rOnSg</recordid><startdate>20200501</startdate><enddate>20200501</enddate><creator>Briones, M. Valentina</creator><creator>Hoenicka, Hans</creator><creator>Cañas, Luis A.</creator><creator>Beltrán, José Pío</creator><creator>Hanelt, Dieter</creator><creator>Sharry, Sandra</creator><creator>Fladung, Matthias</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-9301-8581</orcidid></search><sort><creationdate>20200501</creationdate><title>Efficient evaluation of a gene containment system for poplar through early flowering induction</title><author>Briones, M. Valentina ; Hoenicka, Hans ; Cañas, Luis A. ; Beltrán, José Pío ; Hanelt, Dieter ; Sharry, Sandra ; Fladung, Matthias</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-10229790b363800bedf417be8ba3e9e3eb3628b4b5b46f3d108af641e562a1ae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Arabidopsis Proteins - genetics</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Barnase</topic><topic>Barstar</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cell Biology</topic><topic>Containment</topic><topic>Flowering</topic><topic>Flowers</topic><topic>Flowers - genetics</topic><topic>Flowers - growth & development</topic><topic>Flowers - metabolism</topic><topic>Flowers - radiation effects</topic><topic>Gene Editing - methods</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene Flow</topic><topic>Gene transfer</topic><topic>Genetic Vectors</topic><topic>Heat-Shock Response</topic><topic>Indigenous plants</topic><topic>Indigenous species</topic><topic>Introduced species</topic><topic>Life Sciences</topic><topic>Original</topic><topic>Original Article</topic><topic>Plant Biochemistry</topic><topic>Plant Infertility - genetics</topic><topic>Plant Sciences</topic><topic>Plant species</topic><topic>Plants, Genetically Modified - growth & development</topic><topic>Plants, Genetically Modified - metabolism</topic><topic>Plants, Genetically Modified - radiation effects</topic><topic>Pollen</topic><topic>Pollen - genetics</topic><topic>Pollen - growth & development</topic><topic>Poplar</topic><topic>Populus - genetics</topic><topic>Populus - growth & development</topic><topic>Populus - metabolism</topic><topic>Populus - radiation effects</topic><topic>Promoter Regions, Genetic</topic><topic>Ribonucleases - genetics</topic><topic>Ribonucleases - metabolism</topic><topic>Sterility</topic><topic>Temperature</topic><topic>Transformation, Genetic</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Briones, M. Valentina</creatorcontrib><creatorcontrib>Hoenicka, Hans</creatorcontrib><creatorcontrib>Cañas, Luis A.</creatorcontrib><creatorcontrib>Beltrán, José Pío</creatorcontrib><creatorcontrib>Hanelt, Dieter</creatorcontrib><creatorcontrib>Sharry, Sandra</creatorcontrib><creatorcontrib>Fladung, Matthias</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Briones, M. Valentina</au><au>Hoenicka, Hans</au><au>Cañas, Luis A.</au><au>Beltrán, José Pío</au><au>Hanelt, Dieter</au><au>Sharry, Sandra</au><au>Fladung, Matthias</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Efficient evaluation of a gene containment system for poplar through early flowering induction</atitle><jtitle>Plant cell reports</jtitle><stitle>Plant Cell Rep</stitle><addtitle>Plant Cell Rep</addtitle><date>2020-05-01</date><risdate>2020</risdate><volume>39</volume><issue>5</issue><spage>577</spage><epage>587</epage><pages>577-587</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><abstract>Key message
The early flowering system HSP::
AtFT
allowed a fast evaluation of a gene containment system based on the construct PsEND1::
barnase–barstar
for poplar
.
Transgenic lines showed disturbed pollen development and sterility.
Vertical gene transfer through pollen flow from transgenic or non-native plant species into their crossable natural relatives is a major concern. Gene containment approaches have been proposed to reduce or even avoid gene flow among tree species. However, evaluation of genetic containment strategies for trees is very difficult due to the long-generation times. Early flowering induction would allow faster evaluation of genetic containment in this case. Although no reliable methods were available for the induction of fertile flowers in poplar, recently, a new early flowering approach was developed. In this study, early flowering poplar lines containing the gene construct PsEND1::
barnase–barstar
were obtained. The PsEND1 promoter was chosen due to its early expression pattern, its versality and efficiency for generation of male-sterile plants fused to the
barnase
gene. RT-PCRs confirmed
barnase
gene activity in flowers, and pollen development was disturbed, leading to sterile flowers. The system developed in this study represents a valuable tool for gene containment studies in forest tree species.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>32052127</pmid><doi>10.1007/s00299-020-02515-1</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-9301-8581</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant cell reports, 2020-05, Vol.39 (5), p.577-587 |
| issn | 0721-7714 1432-203X |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7165154 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Arabidopsis Proteins - genetics Bacterial Proteins - genetics Bacterial Proteins - metabolism Barnase Barstar Biomedical and Life Sciences Biotechnology Cell Biology Containment Flowering Flowers Flowers - genetics Flowers - growth & development Flowers - metabolism Flowers - radiation effects Gene Editing - methods Gene Expression Regulation, Plant Gene Flow Gene transfer Genetic Vectors Heat-Shock Response Indigenous plants Indigenous species Introduced species Life Sciences Original Original Article Plant Biochemistry Plant Infertility - genetics Plant Sciences Plant species Plants, Genetically Modified - growth & development Plants, Genetically Modified - metabolism Plants, Genetically Modified - radiation effects Pollen Pollen - genetics Pollen - growth & development Poplar Populus - genetics Populus - growth & development Populus - metabolism Populus - radiation effects Promoter Regions, Genetic Ribonucleases - genetics Ribonucleases - metabolism Sterility Temperature Transformation, Genetic Transgenic plants |
| title | Efficient evaluation of a gene containment system for poplar through early flowering induction |
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