Strategies to Optimize Protein Expression in E. coli

Recombinant protein expression in Escherichia coli (E. coli) is simple, fast, inexpensive, and robust, with the expressed protein comprising up to 50 percent of the total cellular protein. However, it also has disadvantages. For example, the rapidity of bacterial protein expression often results in...

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Veröffentlicht in:	Current Protocols in Protein Science 2010-08, Vol.61 (1), p.5.24.1-5.24.29
Hauptverfasser:	Francis, Dana M., Page, Rebecca
Format:	Artikel
Sprache:	eng
Schlagworte:	active protein Bacteria Biochemistry Biochemistry and Molecular Cell Biology Bioinformatics Cell Culture E. coli E. coli, Plasmids, and Bacteriophages Escherichia coli - genetics Escherichia coli - metabolism expression strains and vectors expression tags folded protein fusion proteins heterologous protein Molecular Biology Production of Recombinant Proteins proteases Protein Analysis Protein Engineering - methods protein expression Protein Folding Protein Production, Purification, and Analysis Proteomics Purification of Recombinant Proteins purification tags Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Solubility Unit
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