Label-Free Identification of Early Stages of Breast Ductal Carcinoma via Multiphoton Microscopy
Breast cancer can be cured by early diagnosis. Appropriate and effective clinical treatment benefits from accurate pathological diagnosis. However, due to the lack of effective screening and diagnostic imaging methods, early stages of breast cancer often progress to malignant breast cancer. In this...
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| Veröffentlicht in: | Scanning 2020, Vol.2020 (2020), p.1-8 |
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| creator | Chen, Jianxin Wang, Chuan Xi, Gangqin Zheng, L. Q. Wang, Shu Kang, Deyong Guo, Wenhui Chen, Zhong Lian, Yuane |
| description | Breast cancer can be cured by early diagnosis. Appropriate and effective clinical treatment benefits from accurate pathological diagnosis. However, due to the lack of effective screening and diagnostic imaging methods, early stages of breast cancer often progress to malignant breast cancer. In this study, multiphoton microscopy (MPM) via two-photon excited fluorescence combined with second-harmonic generation was used for identifying the early stages of breast ductal carcinoma. The results showed differences in both cytological features and collagen distribution among normal breast tissue, atypical ductal hyperplasia, low-grade ductal carcinoma in situ, and high-grade ductal carcinoma in situ with microinvasion. Furthermore, three features extracted from the MPM images were used to describe differences in cytological features, collagen density, and basement membrane circumference in the early stages of breast ductal carcinoma. They revealed that MPM has the ability to identify early stages of breast ductal carcinoma label-free, which would contribute to the early diagnosis and treatment of breast cancer. This study may provide the groundwork for the further application of MPM in the clinic. |
| doi_str_mv | 10.1155/2020/9670514 |
| format | Article |
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Q. ; Wang, Shu ; Kang, Deyong ; Guo, Wenhui ; Chen, Zhong ; Lian, Yuane</creator><contributor>Relucenti, Michela ; Michela Relucenti</contributor><creatorcontrib>Chen, Jianxin ; Wang, Chuan ; Xi, Gangqin ; Zheng, L. Q. ; Wang, Shu ; Kang, Deyong ; Guo, Wenhui ; Chen, Zhong ; Lian, Yuane ; Relucenti, Michela ; Michela Relucenti</creatorcontrib><description>Breast cancer can be cured by early diagnosis. Appropriate and effective clinical treatment benefits from accurate pathological diagnosis. However, due to the lack of effective screening and diagnostic imaging methods, early stages of breast cancer often progress to malignant breast cancer. In this study, multiphoton microscopy (MPM) via two-photon excited fluorescence combined with second-harmonic generation was used for identifying the early stages of breast ductal carcinoma. The results showed differences in both cytological features and collagen distribution among normal breast tissue, atypical ductal hyperplasia, low-grade ductal carcinoma in situ, and high-grade ductal carcinoma in situ with microinvasion. Furthermore, three features extracted from the MPM images were used to describe differences in cytological features, collagen density, and basement membrane circumference in the early stages of breast ductal carcinoma. They revealed that MPM has the ability to identify early stages of breast ductal carcinoma label-free, which would contribute to the early diagnosis and treatment of breast cancer. This study may provide the groundwork for the further application of MPM in the clinic.</description><identifier>ISSN: 0161-0457</identifier><identifier>EISSN: 1932-8745</identifier><identifier>DOI: 10.1155/2020/9670514</identifier><identifier>PMID: 32454928</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Breast cancer ; Collagen ; Diagnosis ; Diagnostic systems ; Extracellular matrix ; Feature extraction ; Fluorescence ; Hyperplasia ; Lasers ; Microscopy ; Second harmonic generation</subject><ispartof>Scanning, 2020, Vol.2020 (2020), p.1-8</ispartof><rights>Copyright © 2020 Zhong Chen et al.</rights><rights>Copyright © 2020 Zhong Chen et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. http://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2020 Zhong Chen et al. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-aa55fa07d9d49f6db7b19e936418ff2013852181d854c3c06195dfa71dc3ef813</citedby><cites>FETCH-LOGICAL-c471t-aa55fa07d9d49f6db7b19e936418ff2013852181d854c3c06195dfa71dc3ef813</cites><orcidid>0000-0001-6823-1030 ; 0000-0001-8519-4462 ; 0000-0002-7888-4569</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7154972/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7154972/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,4009,27902,27903,27904,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32454928$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Relucenti, Michela</contributor><contributor>Michela Relucenti</contributor><creatorcontrib>Chen, Jianxin</creatorcontrib><creatorcontrib>Wang, Chuan</creatorcontrib><creatorcontrib>Xi, Gangqin</creatorcontrib><creatorcontrib>Zheng, L. Q.</creatorcontrib><creatorcontrib>Wang, Shu</creatorcontrib><creatorcontrib>Kang, Deyong</creatorcontrib><creatorcontrib>Guo, Wenhui</creatorcontrib><creatorcontrib>Chen, Zhong</creatorcontrib><creatorcontrib>Lian, Yuane</creatorcontrib><title>Label-Free Identification of Early Stages of Breast Ductal Carcinoma via Multiphoton Microscopy</title><title>Scanning</title><addtitle>Scanning</addtitle><description>Breast cancer can be cured by early diagnosis. Appropriate and effective clinical treatment benefits from accurate pathological diagnosis. However, due to the lack of effective screening and diagnostic imaging methods, early stages of breast cancer often progress to malignant breast cancer. In this study, multiphoton microscopy (MPM) via two-photon excited fluorescence combined with second-harmonic generation was used for identifying the early stages of breast ductal carcinoma. The results showed differences in both cytological features and collagen distribution among normal breast tissue, atypical ductal hyperplasia, low-grade ductal carcinoma in situ, and high-grade ductal carcinoma in situ with microinvasion. Furthermore, three features extracted from the MPM images were used to describe differences in cytological features, collagen density, and basement membrane circumference in the early stages of breast ductal carcinoma. They revealed that MPM has the ability to identify early stages of breast ductal carcinoma label-free, which would contribute to the early diagnosis and treatment of breast cancer. 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Q.</au><au>Wang, Shu</au><au>Kang, Deyong</au><au>Guo, Wenhui</au><au>Chen, Zhong</au><au>Lian, Yuane</au><au>Relucenti, Michela</au><au>Michela Relucenti</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Label-Free Identification of Early Stages of Breast Ductal Carcinoma via Multiphoton Microscopy</atitle><jtitle>Scanning</jtitle><addtitle>Scanning</addtitle><date>2020</date><risdate>2020</risdate><volume>2020</volume><issue>2020</issue><spage>1</spage><epage>8</epage><pages>1-8</pages><issn>0161-0457</issn><eissn>1932-8745</eissn><abstract>Breast cancer can be cured by early diagnosis. Appropriate and effective clinical treatment benefits from accurate pathological diagnosis. However, due to the lack of effective screening and diagnostic imaging methods, early stages of breast cancer often progress to malignant breast cancer. In this study, multiphoton microscopy (MPM) via two-photon excited fluorescence combined with second-harmonic generation was used for identifying the early stages of breast ductal carcinoma. The results showed differences in both cytological features and collagen distribution among normal breast tissue, atypical ductal hyperplasia, low-grade ductal carcinoma in situ, and high-grade ductal carcinoma in situ with microinvasion. Furthermore, three features extracted from the MPM images were used to describe differences in cytological features, collagen density, and basement membrane circumference in the early stages of breast ductal carcinoma. They revealed that MPM has the ability to identify early stages of breast ductal carcinoma label-free, which would contribute to the early diagnosis and treatment of breast cancer. This study may provide the groundwork for the further application of MPM in the clinic.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>32454928</pmid><doi>10.1155/2020/9670514</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-6823-1030</orcidid><orcidid>https://orcid.org/0000-0001-8519-4462</orcidid><orcidid>https://orcid.org/0000-0002-7888-4569</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; Wiley Online Library Open Access; PubMed Central; Alma/SFX Local Collection |
| subjects | Breast cancer Collagen Diagnosis Diagnostic systems Extracellular matrix Feature extraction Fluorescence Hyperplasia Lasers Microscopy Second harmonic generation |
| title | Label-Free Identification of Early Stages of Breast Ductal Carcinoma via Multiphoton Microscopy |
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