Sample deposition onto cryo‐EM grids: from sprays to jets and back

Despite the great strides made in the field of single‐particle cryogenic electron microscopy (cryo‐EM) in microscope design, direct electron detectors and new processing suites, the area of sample preparation is still far from ideal. Traditionally, sample preparation involves blotting, which has bee...

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Veröffentlicht in:	Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 2020-04, Vol.76 (4), p.340-349
Hauptverfasser:	Klebl, David P., Monteiro, Diana C. F., Kontziampasis, Dimitrios, Kopf, Florian, Sobott, Frank, White, Howard D., Trebbin, Martin, Muench, Stephen P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Apoferritin Ccp-EM Cryoelectron Microscopy - methods cryo‐EM Deposition Droplets Electron microscopy gas dynamic virtual nozzle microfluidics Nozzles Production methods Proteins sample preparation Specimen Handling - methods Sprayers structure determination time‐resolved Water damage
Online-Zugang:	Volltext
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