Vaccinia virus produces late mRNAs by discontinuous synthesis
We describe the unusual structure of a vaccinia virus late mRNA. In these molecules, the protein-coding sequences of a major late structural polypeptide are preceded by long leader RNAs, which in some cases are thousands of nucleotides long. These sequences map to different regions of the viral geno...
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| Veröffentlicht in: | Cell 1987-07, Vol.50 (2), p.153-162 |
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| container_title | Cell |
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| creator | Bertholet, Christine Van Meir, Erwin ten Heggeler-Bordier, Béatrice Wittek, Riccardo |
| description | We describe the unusual structure of a vaccinia virus late mRNA. In these molecules, the protein-coding sequences of a major late structural polypeptide are preceded by long leader RNAs, which in some cases are thousands of nucleotides long. These sequences map to different regions of the viral genome and in one instance are separated from the late gene by more than 100 kb of DNA. Moreover, the leader sequences map either upstream or downstream of the late gene, are transcribed from either DNA strand, and are fused to the late gene coding sequence via a poly(A) stretch. This demonstrates that vaccinia virus produces late mRNAs by tagging the protein-coding sequences onto the 3′ end of other RNAs. |
| doi_str_mv | 10.1016/0092-8674(87)90211-X |
| format | Article |
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In these molecules, the protein-coding sequences of a major late structural polypeptide are preceded by long leader RNAs, which in some cases are thousands of nucleotides long. These sequences map to different regions of the viral genome and in one instance are separated from the late gene by more than 100 kb of DNA. Moreover, the leader sequences map either upstream or downstream of the late gene, are transcribed from either DNA strand, and are fused to the late gene coding sequence via a poly(A) stretch. This demonstrates that vaccinia virus produces late mRNAs by tagging the protein-coding sequences onto the 3′ end of other RNAs.</description><identifier>ISSN: 0092-8674</identifier><identifier>EISSN: 1097-4172</identifier><identifier>DOI: 10.1016/0092-8674(87)90211-X</identifier><identifier>PMID: 3036368</identifier><identifier>CODEN: CELLB5</identifier><language>eng</language><publisher>Cambridge, MA: Elsevier Inc</publisher><subject>Base Sequence ; Biological and medical sciences ; Cloning, Molecular ; DNA - metabolism ; DNA Restriction Enzymes ; DNA, Viral - genetics ; Fundamental and applied biological sciences. Psychology ; Genes, Viral ; HeLa Cells - metabolism ; Humans ; Molecular and cellular biology ; Molecular genetics ; Molecular Weight ; Nucleic Acid Hybridization ; RNA, Messenger - biosynthesis ; RNA, Messenger - genetics ; Transcription. Transcription factor. Splicing. Rna processing ; vaccinia virus ; Vaccinia virus - genetics</subject><ispartof>Cell, 1987-07, Vol.50 (2), p.153-162</ispartof><rights>1987</rights><rights>1988 INIST-CNRS</rights><rights>Copyright © 1987 . 1987</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c433x-7bc3dc70b4b2a5f882e0646a9e94a1fe26b6bf202876fb1b299e5778807d3c2e3</citedby><cites>FETCH-LOGICAL-c433x-7bc3dc70b4b2a5f882e0646a9e94a1fe26b6bf202876fb1b299e5778807d3c2e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/009286748790211X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7467553$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3036368$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bertholet, Christine</creatorcontrib><creatorcontrib>Van Meir, Erwin</creatorcontrib><creatorcontrib>ten Heggeler-Bordier, Béatrice</creatorcontrib><creatorcontrib>Wittek, Riccardo</creatorcontrib><title>Vaccinia virus produces late mRNAs by discontinuous synthesis</title><title>Cell</title><addtitle>Cell</addtitle><description>We describe the unusual structure of a vaccinia virus late mRNA. In these molecules, the protein-coding sequences of a major late structural polypeptide are preceded by long leader RNAs, which in some cases are thousands of nucleotides long. These sequences map to different regions of the viral genome and in one instance are separated from the late gene by more than 100 kb of DNA. Moreover, the leader sequences map either upstream or downstream of the late gene, are transcribed from either DNA strand, and are fused to the late gene coding sequence via a poly(A) stretch. This demonstrates that vaccinia virus produces late mRNAs by tagging the protein-coding sequences onto the 3′ end of other RNAs.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>DNA - metabolism</subject><subject>DNA Restriction Enzymes</subject><subject>DNA, Viral - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Viral</subject><subject>HeLa Cells - metabolism</subject><subject>Humans</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Weight</subject><subject>Nucleic Acid Hybridization</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - genetics</subject><subject>Transcription. Transcription factor. Splicing. 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Psychology</topic><topic>Genes, Viral</topic><topic>HeLa Cells - metabolism</topic><topic>Humans</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Weight</topic><topic>Nucleic Acid Hybridization</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RNA, Messenger - genetics</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><topic>vaccinia virus</topic><topic>Vaccinia virus - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bertholet, Christine</creatorcontrib><creatorcontrib>Van Meir, Erwin</creatorcontrib><creatorcontrib>ten Heggeler-Bordier, Béatrice</creatorcontrib><creatorcontrib>Wittek, Riccardo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bertholet, Christine</au><au>Van Meir, Erwin</au><au>ten Heggeler-Bordier, Béatrice</au><au>Wittek, Riccardo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vaccinia virus produces late mRNAs by discontinuous synthesis</atitle><jtitle>Cell</jtitle><addtitle>Cell</addtitle><date>1987-07-17</date><risdate>1987</risdate><volume>50</volume><issue>2</issue><spage>153</spage><epage>162</epage><pages>153-162</pages><issn>0092-8674</issn><eissn>1097-4172</eissn><coden>CELLB5</coden><abstract>We describe the unusual structure of a vaccinia virus late mRNA. In these molecules, the protein-coding sequences of a major late structural polypeptide are preceded by long leader RNAs, which in some cases are thousands of nucleotides long. These sequences map to different regions of the viral genome and in one instance are separated from the late gene by more than 100 kb of DNA. Moreover, the leader sequences map either upstream or downstream of the late gene, are transcribed from either DNA strand, and are fused to the late gene coding sequence via a poly(A) stretch. This demonstrates that vaccinia virus produces late mRNAs by tagging the protein-coding sequences onto the 3′ end of other RNAs.</abstract><cop>Cambridge, MA</cop><pub>Elsevier Inc</pub><pmid>3036368</pmid><doi>10.1016/0092-8674(87)90211-X</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Base Sequence Biological and medical sciences Cloning, Molecular DNA - metabolism DNA Restriction Enzymes DNA, Viral - genetics Fundamental and applied biological sciences. Psychology Genes, Viral HeLa Cells - metabolism Humans Molecular and cellular biology Molecular genetics Molecular Weight Nucleic Acid Hybridization RNA, Messenger - biosynthesis RNA, Messenger - genetics Transcription. Transcription factor. Splicing. Rna processing vaccinia virus Vaccinia virus - genetics |
| title | Vaccinia virus produces late mRNAs by discontinuous synthesis |
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