Primary structure of the membrane and nucleocapsid protein genes of feline infectious peritonitis virus and immunogenicity of recombinant vaccinia viruses in kittens
Feline infectious peritonitis virus (FIPV) causes a mostly fatal, immunologically mediated disease in cats. Previously, we demonstrated that immunization with a recombinant vaccinia virus expressing the FIPV spike protein (S) induced early death after challenge with FIPV (Vennema et al., 1990, J. Vi...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1991-03, Vol.181 (1), p.327-335 |
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| creator | Vennema, Harry De Groot, Raoul J. Harbour, David A. Horzinek, Marian C. Spaan, Willy J.M. |
| description | Feline infectious peritonitis virus (FIPV) causes a mostly fatal, immunologically mediated disease in cats. Previously, we demonstrated that immunization with a recombinant vaccinia virus expressing the FIPV spike protein (S) induced early death after challenge with FIPV (Vennema
et al., 1990,
J. Virol. 64, 1407–1409). In this paper we describe similar immunizations with the FIPV membrane (M) and nucleocapsid (N) proteins. The genes encoding these proteins were cloned and sequenced. Comparison of the amino acid sequences with the corresponding sequences of porcine transmissible gastroenteritis virus revealed 84.7 and 77% identity for M and N, respectively. Vaccinia virus recombinants expressing the cloned genes induced antibodies in immunized kittens. Immunization with neither recombinant induced early death after challenge with FIPV, strongly suggesting that antibody-dependent enhancement is mediated by antibodies against S only. Immunization with the N protein recombinant had no apparent effect on the outcome of challenge. However, three of eight kittens immunized with the M protein recombinant survived the challenge, as compared to one of eight kittens of the control group. |
| doi_str_mv | 10.1016/0042-6822(91)90499-2 |
| format | Article |
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et al., 1990,
J. Virol. 64, 1407–1409). In this paper we describe similar immunizations with the FIPV membrane (M) and nucleocapsid (N) proteins. The genes encoding these proteins were cloned and sequenced. Comparison of the amino acid sequences with the corresponding sequences of porcine transmissible gastroenteritis virus revealed 84.7 and 77% identity for M and N, respectively. Vaccinia virus recombinants expressing the cloned genes induced antibodies in immunized kittens. Immunization with neither recombinant induced early death after challenge with FIPV, strongly suggesting that antibody-dependent enhancement is mediated by antibodies against S only. Immunization with the N protein recombinant had no apparent effect on the outcome of challenge. However, three of eight kittens immunized with the M protein recombinant survived the challenge, as compared to one of eight kittens of the control group.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/0042-6822(91)90499-2</identifier><identifier>PMID: 1847259</identifier><identifier>CODEN: VIRLAX</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; Capsid - genetics ; Cats ; Cell Line ; CHATON ; CLONE ; CLONES ; Cloning, Molecular ; CORONAVIRIDAE ; Coronaviridae - genetics ; Coronaviridae - immunology ; Coronaviridae Infections - immunology ; Fundamental and applied biological sciences. Psychology ; GATITO ; GENE ; GENES ; Genes, Viral ; IMMUNISATION ; Immunization ; INMUNIZACION ; Microbiology ; Molecular Sequence Data ; NUCLEOTIDE ; NUCLEOTIDOS ; ORTHOPOXVIRUS ; Peritonitis - immunology ; Peritonitis - microbiology ; PROTEINAS ; PROTEINE ; RECOMBINACION ; RECOMBINAISON ; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains ; REPONSE IMMUNITAIRE ; RESPUESTA INMUNOLOGICA ; Restriction Mapping ; Sequence Homology, Nucleic Acid ; SUPERVIVENCIA ; SURVIE ; vaccinia virus ; Vaccinia virus - genetics ; Vaccinia virus - immunology ; Viral Core Proteins - genetics ; Viral Proteins - genetics ; Virology ; VIRUS</subject><ispartof>Virology (New York, N.Y.), 1991-03, Vol.181 (1), p.327-335</ispartof><rights>1991</rights><rights>1991 INIST-CNRS</rights><rights>Copyright © 1991 Published by Elsevier Inc. 1991</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c538t-bb463baf02c527a533443e6a588d5d186754944f93b9e956c30c5f48cf8c7c443</citedby><cites>FETCH-LOGICAL-c538t-bb463baf02c527a533443e6a588d5d186754944f93b9e956c30c5f48cf8c7c443</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0042682291904992$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19737287$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1847259$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vennema, Harry</creatorcontrib><creatorcontrib>De Groot, Raoul J.</creatorcontrib><creatorcontrib>Harbour, David A.</creatorcontrib><creatorcontrib>Horzinek, Marian C.</creatorcontrib><creatorcontrib>Spaan, Willy J.M.</creatorcontrib><title>Primary structure of the membrane and nucleocapsid protein genes of feline infectious peritonitis virus and immunogenicity of recombinant vaccinia viruses in kittens</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>Feline infectious peritonitis virus (FIPV) causes a mostly fatal, immunologically mediated disease in cats. Previously, we demonstrated that immunization with a recombinant vaccinia virus expressing the FIPV spike protein (S) induced early death after challenge with FIPV (Vennema
et al., 1990,
J. Virol. 64, 1407–1409). In this paper we describe similar immunizations with the FIPV membrane (M) and nucleocapsid (N) proteins. The genes encoding these proteins were cloned and sequenced. Comparison of the amino acid sequences with the corresponding sequences of porcine transmissible gastroenteritis virus revealed 84.7 and 77% identity for M and N, respectively. Vaccinia virus recombinants expressing the cloned genes induced antibodies in immunized kittens. Immunization with neither recombinant induced early death after challenge with FIPV, strongly suggesting that antibody-dependent enhancement is mediated by antibodies against S only. Immunization with the N protein recombinant had no apparent effect on the outcome of challenge. However, three of eight kittens immunized with the M protein recombinant survived the challenge, as compared to one of eight kittens of the control group.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Capsid - genetics</subject><subject>Cats</subject><subject>Cell Line</subject><subject>CHATON</subject><subject>CLONE</subject><subject>CLONES</subject><subject>Cloning, Molecular</subject><subject>CORONAVIRIDAE</subject><subject>Coronaviridae - genetics</subject><subject>Coronaviridae - immunology</subject><subject>Coronaviridae Infections - immunology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GATITO</subject><subject>GENE</subject><subject>GENES</subject><subject>Genes, Viral</subject><subject>IMMUNISATION</subject><subject>Immunization</subject><subject>INMUNIZACION</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>NUCLEOTIDE</subject><subject>NUCLEOTIDOS</subject><subject>ORTHOPOXVIRUS</subject><subject>Peritonitis - immunology</subject><subject>Peritonitis - microbiology</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>RECOMBINACION</subject><subject>RECOMBINAISON</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>REPONSE IMMUNITAIRE</subject><subject>RESPUESTA INMUNOLOGICA</subject><subject>Restriction Mapping</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>SUPERVIVENCIA</subject><subject>SURVIE</subject><subject>vaccinia virus</subject><subject>Vaccinia virus - genetics</subject><subject>Vaccinia virus - immunology</subject><subject>Viral Core Proteins - genetics</subject><subject>Viral Proteins - genetics</subject><subject>Virology</subject><subject>VIRUS</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks1u1DAUhSMEKtPCCyCQsqGCRcCOndjeIKGKP6kSSNC15Tg30wuJPbWdkfpAvCcOGXVgAyvLvt85vr7HRfGUkleU0PY1IbyuWlnXLxR9qQhXqqrvFRtKVFsRxun9YnOHPCxOY_xO8l4IclKcUMlF3ahN8fNLwMmE2zKmMNs0Byj9UKZrKCeYumAclMb1pZvtCN6aXcS-3AWfAF25BQdxwQcYMYPoBrAJ_RzLHQRM3mHCWO4x5JPFBadpdj7L0GK6XZQBrJ86dMalcm-sRYdmFWTnfMUPTAlcfFQ8GMwY4fFhPSuu3r_7dvGxuvz84dPF28vKNkymqut4yzozkNo2tTANY5wzaE0jZd_0VLai4YrzQbFOgWpay4htBi7tIK2wmT0r3qy-u7mboLfgUjCj3q0z0t6g_rvi8Fpv_V4LyoikIhucHwyCv5khJj1htDCOeZB5LloSzrkg_L8gbQmtWUMzyFfQBh9jgOGuG0r08g_0ErJeQtaK6t__QNdZ9uzPlxxFa_C5_vxQN9GacchJW4xHTAkmark86MnKDcZrsw2ZufqqaM0ZF8dxQc5kjxB0tAjOQo852KR7j__u8heRYtyP</recordid><startdate>19910301</startdate><enddate>19910301</enddate><creator>Vennema, Harry</creator><creator>De Groot, Raoul J.</creator><creator>Harbour, David A.</creator><creator>Horzinek, Marian C.</creator><creator>Spaan, Willy J.M.</creator><general>Elsevier Inc</general><general>Elsevier</general><general>Published by Elsevier Inc</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19910301</creationdate><title>Primary structure of the membrane and nucleocapsid protein genes of feline infectious peritonitis virus and immunogenicity of recombinant vaccinia viruses in kittens</title><author>Vennema, Harry ; De Groot, Raoul J. ; Harbour, David A. ; Horzinek, Marian C. ; Spaan, Willy J.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c538t-bb463baf02c527a533443e6a588d5d186754944f93b9e956c30c5f48cf8c7c443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Capsid - genetics</topic><topic>Cats</topic><topic>Cell Line</topic><topic>CHATON</topic><topic>CLONE</topic><topic>CLONES</topic><topic>Cloning, Molecular</topic><topic>CORONAVIRIDAE</topic><topic>Coronaviridae - genetics</topic><topic>Coronaviridae - immunology</topic><topic>Coronaviridae Infections - immunology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GATITO</topic><topic>GENE</topic><topic>GENES</topic><topic>Genes, Viral</topic><topic>IMMUNISATION</topic><topic>Immunization</topic><topic>INMUNIZACION</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>NUCLEOTIDE</topic><topic>NUCLEOTIDOS</topic><topic>ORTHOPOXVIRUS</topic><topic>Peritonitis - immunology</topic><topic>Peritonitis - microbiology</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>RECOMBINACION</topic><topic>RECOMBINAISON</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>REPONSE IMMUNITAIRE</topic><topic>RESPUESTA INMUNOLOGICA</topic><topic>Restriction Mapping</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>SUPERVIVENCIA</topic><topic>SURVIE</topic><topic>vaccinia virus</topic><topic>Vaccinia virus - genetics</topic><topic>Vaccinia virus - immunology</topic><topic>Viral Core Proteins - genetics</topic><topic>Viral Proteins - genetics</topic><topic>Virology</topic><topic>VIRUS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vennema, Harry</creatorcontrib><creatorcontrib>De Groot, Raoul J.</creatorcontrib><creatorcontrib>Harbour, David A.</creatorcontrib><creatorcontrib>Horzinek, Marian C.</creatorcontrib><creatorcontrib>Spaan, Willy J.M.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vennema, Harry</au><au>De Groot, Raoul J.</au><au>Harbour, David A.</au><au>Horzinek, Marian C.</au><au>Spaan, Willy J.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Primary structure of the membrane and nucleocapsid protein genes of feline infectious peritonitis virus and immunogenicity of recombinant vaccinia viruses in kittens</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1991-03-01</date><risdate>1991</risdate><volume>181</volume><issue>1</issue><spage>327</spage><epage>335</epage><pages>327-335</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><coden>VIRLAX</coden><abstract>Feline infectious peritonitis virus (FIPV) causes a mostly fatal, immunologically mediated disease in cats. Previously, we demonstrated that immunization with a recombinant vaccinia virus expressing the FIPV spike protein (S) induced early death after challenge with FIPV (Vennema
et al., 1990,
J. Virol. 64, 1407–1409). In this paper we describe similar immunizations with the FIPV membrane (M) and nucleocapsid (N) proteins. The genes encoding these proteins were cloned and sequenced. Comparison of the amino acid sequences with the corresponding sequences of porcine transmissible gastroenteritis virus revealed 84.7 and 77% identity for M and N, respectively. Vaccinia virus recombinants expressing the cloned genes induced antibodies in immunized kittens. Immunization with neither recombinant induced early death after challenge with FIPV, strongly suggesting that antibody-dependent enhancement is mediated by antibodies against S only. Immunization with the N protein recombinant had no apparent effect on the outcome of challenge. However, three of eight kittens immunized with the M protein recombinant survived the challenge, as compared to one of eight kittens of the control group.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1847259</pmid><doi>10.1016/0042-6822(91)90499-2</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0042-6822 |
| ispartof | Virology (New York, N.Y.), 1991-03, Vol.181 (1), p.327-335 |
| issn | 0042-6822 1096-0341 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7130817 |
| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Amino Acid Sequence Animals Base Sequence Biological and medical sciences Capsid - genetics Cats Cell Line CHATON CLONE CLONES Cloning, Molecular CORONAVIRIDAE Coronaviridae - genetics Coronaviridae - immunology Coronaviridae Infections - immunology Fundamental and applied biological sciences. Psychology GATITO GENE GENES Genes, Viral IMMUNISATION Immunization INMUNIZACION Microbiology Molecular Sequence Data NUCLEOTIDE NUCLEOTIDOS ORTHOPOXVIRUS Peritonitis - immunology Peritonitis - microbiology PROTEINAS PROTEINE RECOMBINACION RECOMBINAISON Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains REPONSE IMMUNITAIRE RESPUESTA INMUNOLOGICA Restriction Mapping Sequence Homology, Nucleic Acid SUPERVIVENCIA SURVIE vaccinia virus Vaccinia virus - genetics Vaccinia virus - immunology Viral Core Proteins - genetics Viral Proteins - genetics Virology VIRUS |
| title | Primary structure of the membrane and nucleocapsid protein genes of feline infectious peritonitis virus and immunogenicity of recombinant vaccinia viruses in kittens |
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