Successful cross-protective efficacy induced by heat-adapted live attenuated nephropathogenic infectious bronchitis virus derived from a natural recombinant strain
Abstract A natural recombinant nephropathogenic K40/09 strain of infectious bronchitis virus (IBV) was heat-adapted for possible future use as live attenuated vaccine. The K40/09 strain was selected during successive serial passages in specific-pathogen free (SPF) embryonated eggs at sub-optimal hig...
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| Veröffentlicht in: | Vaccine 2015-12, Vol.33 (51), p.7370-7374 |
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| creator | Lim, Tae-Hyun Youn, Ha-Na Yuk, Seong-Su Kwon, Jung-Hoon Hong, Woo-Tack Gwon, Gyeong-Bin Lee, Jung-Ah Lee, Joong-Bok Lee, Sang-Won Song, Chang-Seon |
| description | Abstract A natural recombinant nephropathogenic K40/09 strain of infectious bronchitis virus (IBV) was heat-adapted for possible future use as live attenuated vaccine. The K40/09 strain was selected during successive serial passages in specific-pathogen free (SPF) embryonated eggs at sub-optimal higher temperature (56 °C). Unlike the parental strain, the attenuated strain, designated K40/09 HP50, was found to be safe in 1-day-old SPF chicks, which showed neither mortality nor signs of morbidity, and rarely induced ciliostasis or histological changes in the trachea and kidney after intraocular and fine-spray administration. K40/09 HP50 provided almost complete protection against two distinct subgroups of a nephropathogenic strain (KM91-like and QX-like subgroup) and elicited the production of high titers of neutralizing antibody (neutralization index of 3.6). We conclude that the K40/09 HP50 vaccine virus is rapidly attenuated by heat adaptation and exhibits the desired level of attenuation, immunogenicity, and protective efficacy required for a live attenuated vaccine. These results indicate that the K40/09 vaccine could be helpful for the reduction of economic losses caused by recently emergent nephropathogenic IBV infection in many countries. |
| doi_str_mv | 10.1016/j.vaccine.2015.07.043 |
| format | Article |
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The K40/09 strain was selected during successive serial passages in specific-pathogen free (SPF) embryonated eggs at sub-optimal higher temperature (56 °C). Unlike the parental strain, the attenuated strain, designated K40/09 HP50, was found to be safe in 1-day-old SPF chicks, which showed neither mortality nor signs of morbidity, and rarely induced ciliostasis or histological changes in the trachea and kidney after intraocular and fine-spray administration. K40/09 HP50 provided almost complete protection against two distinct subgroups of a nephropathogenic strain (KM91-like and QX-like subgroup) and elicited the production of high titers of neutralizing antibody (neutralization index of 3.6). We conclude that the K40/09 HP50 vaccine virus is rapidly attenuated by heat adaptation and exhibits the desired level of attenuation, immunogenicity, and protective efficacy required for a live attenuated vaccine. These results indicate that the K40/09 vaccine could be helpful for the reduction of economic losses caused by recently emergent nephropathogenic IBV infection in many countries.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2015.07.043</identifier><identifier>PMID: 26218899</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject><![CDATA[Adaptation, Biological ; Allergy and Immunology ; Animal diseases ; Animal vaccines ; Animals ; Attenuation ; Chickens ; Coronavirus Infections - prevention & control ; Coronavirus Infections - veterinary ; Cross-protective efficacy ; Disease control ; Heat adaptation ; High temperature ; Hot Temperature ; Immunization ; Immunogenicity ; Infectious bronchitis virus ; Infectious bronchitis virus - immunology ; Infectious bronchitis virus - isolation & purification ; Kidney - pathology ; Kidneys ; Laboratory animals ; Neutralization ; Poultry ; Poultry Diseases - immunology ; Poultry Diseases - prevention & control ; RNA polymerase ; Safety ; Serial Passage ; Studies ; Survival Analysis ; Trachea - pathology ; Vaccines ; Vaccines, Attenuated - administration & dosage ; Vaccines, Attenuated - adverse effects ; Vaccines, Attenuated - immunology ; Vaccines, Attenuated - isolation & purification ; Viral Vaccines - administration & dosage ; Viral Vaccines - adverse effects ; Viral Vaccines - immunology ; Viral Vaccines - isolation & purification ; Viruses]]></subject><ispartof>Vaccine, 2015-12, Vol.33 (51), p.7370-7374</ispartof><rights>Elsevier Ltd</rights><rights>2015 Elsevier Ltd</rights><rights>Copyright © 2015 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Limited Dec 16, 2015</rights><rights>Copyright © 2015 Elsevier Ltd. All rights reserved. 2015 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c653t-3385420f7f5b87707c3e11c69e98381d31be861b4e6a07aecc75bffc0fb715423</citedby><cites>FETCH-LOGICAL-c653t-3385420f7f5b87707c3e11c69e98381d31be861b4e6a07aecc75bffc0fb715423</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1746587045?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995,64385,64387,64389,72469</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26218899$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lim, Tae-Hyun</creatorcontrib><creatorcontrib>Youn, Ha-Na</creatorcontrib><creatorcontrib>Yuk, Seong-Su</creatorcontrib><creatorcontrib>Kwon, Jung-Hoon</creatorcontrib><creatorcontrib>Hong, Woo-Tack</creatorcontrib><creatorcontrib>Gwon, Gyeong-Bin</creatorcontrib><creatorcontrib>Lee, Jung-Ah</creatorcontrib><creatorcontrib>Lee, Joong-Bok</creatorcontrib><creatorcontrib>Lee, Sang-Won</creatorcontrib><creatorcontrib>Song, Chang-Seon</creatorcontrib><title>Successful cross-protective efficacy induced by heat-adapted live attenuated nephropathogenic infectious bronchitis virus derived from a natural recombinant strain</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Abstract A natural recombinant nephropathogenic K40/09 strain of infectious bronchitis virus (IBV) was heat-adapted for possible future use as live attenuated vaccine. The K40/09 strain was selected during successive serial passages in specific-pathogen free (SPF) embryonated eggs at sub-optimal higher temperature (56 °C). Unlike the parental strain, the attenuated strain, designated K40/09 HP50, was found to be safe in 1-day-old SPF chicks, which showed neither mortality nor signs of morbidity, and rarely induced ciliostasis or histological changes in the trachea and kidney after intraocular and fine-spray administration. K40/09 HP50 provided almost complete protection against two distinct subgroups of a nephropathogenic strain (KM91-like and QX-like subgroup) and elicited the production of high titers of neutralizing antibody (neutralization index of 3.6). We conclude that the K40/09 HP50 vaccine virus is rapidly attenuated by heat adaptation and exhibits the desired level of attenuation, immunogenicity, and protective efficacy required for a live attenuated vaccine. These results indicate that the K40/09 vaccine could be helpful for the reduction of economic losses caused by recently emergent nephropathogenic IBV infection in many countries.</description><subject>Adaptation, Biological</subject><subject>Allergy and Immunology</subject><subject>Animal diseases</subject><subject>Animal vaccines</subject><subject>Animals</subject><subject>Attenuation</subject><subject>Chickens</subject><subject>Coronavirus Infections - prevention & control</subject><subject>Coronavirus Infections - veterinary</subject><subject>Cross-protective efficacy</subject><subject>Disease control</subject><subject>Heat adaptation</subject><subject>High temperature</subject><subject>Hot Temperature</subject><subject>Immunization</subject><subject>Immunogenicity</subject><subject>Infectious bronchitis virus</subject><subject>Infectious bronchitis virus - immunology</subject><subject>Infectious bronchitis virus - isolation & purification</subject><subject>Kidney - pathology</subject><subject>Kidneys</subject><subject>Laboratory animals</subject><subject>Neutralization</subject><subject>Poultry</subject><subject>Poultry Diseases - immunology</subject><subject>Poultry Diseases - prevention & control</subject><subject>RNA polymerase</subject><subject>Safety</subject><subject>Serial Passage</subject><subject>Studies</subject><subject>Survival Analysis</subject><subject>Trachea - pathology</subject><subject>Vaccines</subject><subject>Vaccines, Attenuated - administration & dosage</subject><subject>Vaccines, Attenuated - adverse effects</subject><subject>Vaccines, Attenuated - immunology</subject><subject>Vaccines, Attenuated - isolation & purification</subject><subject>Viral Vaccines - administration & dosage</subject><subject>Viral Vaccines - adverse effects</subject><subject>Viral Vaccines - immunology</subject><subject>Viral Vaccines - isolation & purification</subject><subject>Viruses</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkt-O1CAUxhujccfVR9CQeONNx0Mphd6sMRv_JZt4sZp4Ryg93WHsQAU6yTyPLyp1xlX3Rq_Ige_7Dhx-RfGUwpoCbV5u13ttjHW4roDyNYg11OxesaJSsLLiVN4vVlA1dVlT-HJWPIpxCwCc0fZhcVY1FZWybVfF9-vZGIxxmEdigo-xnIJPaJLdI8FhsEabA7Gunw32pDuQDepU6l5PKdfjotIpoZv1UjucNsFPOm38DTprsnFYsvwcSRe8MxubbCR7G_JGjyHbezIEvyOaOJ3moEcS0PhdZ512icQUtHWPiweDHiM-Oa3nxee3bz5dvi-vPr77cPn6qjQNZ6lkTPK6gkEMvJNCgDAMKTVNi61kkvaMdigb2tXYaBAajRG8GwYDQydodrLz4uKYO83dDnuDLrcf1RTsToeD8tqqv0-c3agbv1eCMhCS5oAXp4Dgv80Yk9rZaHActcM8AkUFp1XVguT_I4W6akQjsvT5HenWz8HlSWRV3XApoF4C-VH18xcDDrf3pqAWYtRWnYhRCzEKhMrEZN-zPx996_qFSBa8Ogowj35vMahoLLqMg81flVTv7T9bXNxJMKPNdOjxKx4w_n6NipUCdb1gu1BLOVDIBfsBFufu3w</recordid><startdate>20151216</startdate><enddate>20151216</enddate><creator>Lim, Tae-Hyun</creator><creator>Youn, Ha-Na</creator><creator>Yuk, Seong-Su</creator><creator>Kwon, Jung-Hoon</creator><creator>Hong, Woo-Tack</creator><creator>Gwon, Gyeong-Bin</creator><creator>Lee, Jung-Ah</creator><creator>Lee, Joong-Bok</creator><creator>Lee, Sang-Won</creator><creator>Song, Chang-Seon</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20151216</creationdate><title>Successful cross-protective efficacy induced by heat-adapted live attenuated nephropathogenic infectious bronchitis virus derived from a natural recombinant strain</title><author>Lim, Tae-Hyun ; Youn, Ha-Na ; Yuk, Seong-Su ; Kwon, Jung-Hoon ; Hong, Woo-Tack ; Gwon, Gyeong-Bin ; Lee, Jung-Ah ; Lee, Joong-Bok ; Lee, Sang-Won ; Song, Chang-Seon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c653t-3385420f7f5b87707c3e11c69e98381d31be861b4e6a07aecc75bffc0fb715423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adaptation, Biological</topic><topic>Allergy and Immunology</topic><topic>Animal diseases</topic><topic>Animal vaccines</topic><topic>Animals</topic><topic>Attenuation</topic><topic>Chickens</topic><topic>Coronavirus Infections - prevention & control</topic><topic>Coronavirus Infections - veterinary</topic><topic>Cross-protective efficacy</topic><topic>Disease control</topic><topic>Heat adaptation</topic><topic>High temperature</topic><topic>Hot Temperature</topic><topic>Immunization</topic><topic>Immunogenicity</topic><topic>Infectious bronchitis virus</topic><topic>Infectious bronchitis virus - immunology</topic><topic>Infectious bronchitis virus - isolation & purification</topic><topic>Kidney - pathology</topic><topic>Kidneys</topic><topic>Laboratory animals</topic><topic>Neutralization</topic><topic>Poultry</topic><topic>Poultry Diseases - immunology</topic><topic>Poultry Diseases - prevention & control</topic><topic>RNA polymerase</topic><topic>Safety</topic><topic>Serial Passage</topic><topic>Studies</topic><topic>Survival Analysis</topic><topic>Trachea - pathology</topic><topic>Vaccines</topic><topic>Vaccines, Attenuated - administration & dosage</topic><topic>Vaccines, Attenuated - adverse effects</topic><topic>Vaccines, Attenuated - immunology</topic><topic>Vaccines, Attenuated - isolation & purification</topic><topic>Viral Vaccines - administration & dosage</topic><topic>Viral Vaccines - adverse effects</topic><topic>Viral Vaccines - immunology</topic><topic>Viral Vaccines - isolation & purification</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lim, Tae-Hyun</creatorcontrib><creatorcontrib>Youn, Ha-Na</creatorcontrib><creatorcontrib>Yuk, Seong-Su</creatorcontrib><creatorcontrib>Kwon, Jung-Hoon</creatorcontrib><creatorcontrib>Hong, Woo-Tack</creatorcontrib><creatorcontrib>Gwon, Gyeong-Bin</creatorcontrib><creatorcontrib>Lee, Jung-Ah</creatorcontrib><creatorcontrib>Lee, Joong-Bok</creatorcontrib><creatorcontrib>Lee, Sang-Won</creatorcontrib><creatorcontrib>Song, Chang-Seon</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nursing & Allied Health Database</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest_Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Healthcare Administration Database (Alumni)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Healthcare Administration Database</collection><collection>Medical Database</collection><collection>ProQuest Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lim, Tae-Hyun</au><au>Youn, Ha-Na</au><au>Yuk, Seong-Su</au><au>Kwon, Jung-Hoon</au><au>Hong, Woo-Tack</au><au>Gwon, Gyeong-Bin</au><au>Lee, Jung-Ah</au><au>Lee, Joong-Bok</au><au>Lee, Sang-Won</au><au>Song, Chang-Seon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Successful cross-protective efficacy induced by heat-adapted live attenuated nephropathogenic infectious bronchitis virus derived from a natural recombinant strain</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2015-12-16</date><risdate>2015</risdate><volume>33</volume><issue>51</issue><spage>7370</spage><epage>7374</epage><pages>7370-7374</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><abstract>Abstract A natural recombinant nephropathogenic K40/09 strain of infectious bronchitis virus (IBV) was heat-adapted for possible future use as live attenuated vaccine. The K40/09 strain was selected during successive serial passages in specific-pathogen free (SPF) embryonated eggs at sub-optimal higher temperature (56 °C). Unlike the parental strain, the attenuated strain, designated K40/09 HP50, was found to be safe in 1-day-old SPF chicks, which showed neither mortality nor signs of morbidity, and rarely induced ciliostasis or histological changes in the trachea and kidney after intraocular and fine-spray administration. K40/09 HP50 provided almost complete protection against two distinct subgroups of a nephropathogenic strain (KM91-like and QX-like subgroup) and elicited the production of high titers of neutralizing antibody (neutralization index of 3.6). We conclude that the K40/09 HP50 vaccine virus is rapidly attenuated by heat adaptation and exhibits the desired level of attenuation, immunogenicity, and protective efficacy required for a live attenuated vaccine. These results indicate that the K40/09 vaccine could be helpful for the reduction of economic losses caused by recently emergent nephropathogenic IBV infection in many countries.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>26218899</pmid><doi>10.1016/j.vaccine.2015.07.043</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Vaccine, 2015-12, Vol.33 (51), p.7370-7374 |
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| subjects | Adaptation, Biological Allergy and Immunology Animal diseases Animal vaccines Animals Attenuation Chickens Coronavirus Infections - prevention & control Coronavirus Infections - veterinary Cross-protective efficacy Disease control Heat adaptation High temperature Hot Temperature Immunization Immunogenicity Infectious bronchitis virus Infectious bronchitis virus - immunology Infectious bronchitis virus - isolation & purification Kidney - pathology Kidneys Laboratory animals Neutralization Poultry Poultry Diseases - immunology Poultry Diseases - prevention & control RNA polymerase Safety Serial Passage Studies Survival Analysis Trachea - pathology Vaccines Vaccines, Attenuated - administration & dosage Vaccines, Attenuated - adverse effects Vaccines, Attenuated - immunology Vaccines, Attenuated - isolation & purification Viral Vaccines - administration & dosage Viral Vaccines - adverse effects Viral Vaccines - immunology Viral Vaccines - isolation & purification Viruses |
| title | Successful cross-protective efficacy induced by heat-adapted live attenuated nephropathogenic infectious bronchitis virus derived from a natural recombinant strain |
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