The activation-dependent induction of APN-(CD13) in T-cells is controlled at different levels of gene expression

Recently, it was shown that aminopeptidase N (E.C. 3.4.11.2, CD13) is up-regulated during mitogenic stimulation of peripheral T-cells. In this study, we demonstrate that the half-life of APN mRNA was considerably prolonged in these cells leading to a 2.7-fold increase of APN transcript level. The ap...

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Veröffentlicht in:	FEBS letters 1997-07, Vol.412 (1), p.53-56
Hauptverfasser:	Wex, Th, Bühling, F, Arndt, M, Frank, K, Ansorge, S, Lendeckel, U
Format:	Artikel
Sprache:	eng
Schlagworte:	Actinomycin D Ala-pNA alanine-p-nitroanilid aminopeptidase N APN Binding, Competitive CD13 CD13 Antigens - biosynthesis CD13 Antigens - genetics Cell Line cluster of differentation counts per minute cpm Dactinomycin - pharmacology E.C. 3.4.11.2 EDTA Enzyme Activation Enzyme Induction Enzyme Stability ethylenediaminetetraacetic acid Half-Life Half-life time Kinetics mab monoclonal antibody PBS PHA phorbol 12-acetate 13-myristate phosphate-buffered saline phytohemagglutinine PMA Polymerase Chain Reaction Promoter Regions, Genetic reverse transcription-polymerase chain reaction RNA, Messenger - metabolism RT-PCR SDS sodium dodecyl sulfate SSC standard sodium citrate T-Lymphocytes - enzymology
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