Cell culture isolation and sequence analysis of genetically diverse US porcine epidemic diarrhea virus strains including a novel strain with a large deletion in the spike gene

•We isolated a novel PEDV strain with a large deletion (197aa) in the spike gene.•We isolated US PEDV INDEL variant in Vero cells.•We isolated multiple original highly virulent US PEDV strains in Vero cells.•Genomic sequences of the isolated PEDV strains were analyzed.•The isolated genetically diver...

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Veröffentlicht in:	Veterinary microbiology 2014-10, Vol.173 (3-4), p.258-269
Hauptverfasser:	Oka, Tomoichiro, Saif, Linda J., Marthaler, Douglas, Esseili, Malak A., Meulia, Tea, Lin, Chun-Ming, Vlasova, Anastasia N., Jung, Kwonil, Zhang, Yan, Wang, Qiuhong
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Cell culture isolation Cell Culture Techniques - veterinary Chlorocebus aethiops Complete genome analysis Coronavirus Infections - epidemiology Coronavirus Infections - veterinary DNA Primers - genetics Fluorescent Antibody Technique, Indirect - veterinary Genetic Variation Large deletion in S protein Microscopy, Electron, Transmission - veterinary Midwestern United States - epidemiology Molecular Sequence Data PEDV variants Phylogeny Porcine epidemic diarrhea virus (PEDV) Porcine epidemic diarrhea virus - genetics Porcine epidemic diarrhea virus - isolation & purification Porcine epidemic diarrhea virus - pathogenicity Real-Time Polymerase Chain Reaction - veterinary Reverse Transcriptase Polymerase Chain Reaction - veterinary Sequence Analysis, DNA - veterinary Sequence Deletion - genetics Species Specificity Spike Glycoprotein, Coronavirus - genetics Swine Swine Diseases - epidemiology Swine Diseases - virology Vero Cells Virulence
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