Absence of Evidence of Zika Virus Infection in Cord Blood and Urine from Newborns with Congenital Abnormalities, Indonesia

Zika virus (ZIKV) has recently been confirmed as endemic in Indonesia, but no congenital anomalies (CA) related to ZIKV infection have been reported. We performed molecular and serological testing for ZIKV and other flaviviruses on cord serum and urine samples collected in October 2016 to April 2017...

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Veröffentlicht in:The American journal of tropical medicine and hygiene 2020-04, Vol.102 (4), p.876-879
Hauptverfasser: Putri, Nina Dwi, Dhenni, Rama, Handryastuti, Setyo, Johar, Edison, Ma'roef, Chairin Nisa, Fadhilah, Araniy, Perma Iskandar, Adhi Teguh, Prayitno, Ari, Karyanti, Mulya Rahma, Satari, Hindra Irawan, Jumiyanti, Niphidiah, Aprilia, Yuni Yudha, Sriyani, Ida Yus, Dewi, Yora Permata, Yudhaputri, Frilasita A, Safari, Dodi, Hadinegoro, Sri Rezeki, Rosenberg, Ronald, Powers, Ann M, Aye Myint, Khin Saw
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container_title The American journal of tropical medicine and hygiene
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creator Putri, Nina Dwi
Dhenni, Rama
Handryastuti, Setyo
Johar, Edison
Ma'roef, Chairin Nisa
Fadhilah, Araniy
Perma Iskandar, Adhi Teguh
Prayitno, Ari
Karyanti, Mulya Rahma
Satari, Hindra Irawan
Jumiyanti, Niphidiah
Aprilia, Yuni Yudha
Sriyani, Ida Yus
Dewi, Yora Permata
Yudhaputri, Frilasita A
Safari, Dodi
Hadinegoro, Sri Rezeki
Rosenberg, Ronald
Powers, Ann M
Aye Myint, Khin Saw
description Zika virus (ZIKV) has recently been confirmed as endemic in Indonesia, but no congenital anomalies (CA) related to ZIKV infection have been reported. We performed molecular and serological testing for ZIKV and other flaviviruses on cord serum and urine samples collected in October 2016 to April 2017 during a prospective, cross-sectional study of neonates in Jakarta, Indonesia. Of a total of 429 neonates, 53 had CA, including 14 with microcephaly. These 53, and 113 neonate controls without evidence of CA, were tested by ZIKV-specific real-time reverse transcription polymerase chain reaction (RT-PCR), pan-flavivirus RT-PCR, anti-ZIKV and anti-DENV IgM ELISA, and plaque reduction neutralization test. There was no evidence of ZIKV infection among neonates in either the CA or non-CA cohorts, except in three cases with low titers of anti-ZIKV neutralizing antibodies. Further routine evaluation throughout Indonesia of pregnant women and their newborns for exposure to ZIKV should be a high priority for determining risk.
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subjects Adult
Antibodies, Viral - blood
Congenital Abnormalities - blood
Congenital Abnormalities - etiology
Congenital Abnormalities - urine
Congenital Abnormalities - virology
Congenital diseases
Female
Fetal Blood - virology
Humans
Immunoglobulin M - blood
Immunoglobulin M - urine
Indonesia - epidemiology
Infant, Newborn
Male
Newborn babies
Pregnancy
Pregnancy Complications, Infectious - blood
Pregnancy Complications, Infectious - urine
Pregnancy Complications, Infectious - virology
Serology
Viral infections
Young Adult
Zika virus
Zika Virus - isolation & purification
Zika Virus Infection - blood
Zika Virus Infection - urine
Zika Virus Infection - virology
title Absence of Evidence of Zika Virus Infection in Cord Blood and Urine from Newborns with Congenital Abnormalities, Indonesia
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