Absence of Evidence of Zika Virus Infection in Cord Blood and Urine from Newborns with Congenital Abnormalities, Indonesia
Zika virus (ZIKV) has recently been confirmed as endemic in Indonesia, but no congenital anomalies (CA) related to ZIKV infection have been reported. We performed molecular and serological testing for ZIKV and other flaviviruses on cord serum and urine samples collected in October 2016 to April 2017...
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Veröffentlicht in: | The American journal of tropical medicine and hygiene 2020-04, Vol.102 (4), p.876-879 |
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creator | Putri, Nina Dwi Dhenni, Rama Handryastuti, Setyo Johar, Edison Ma'roef, Chairin Nisa Fadhilah, Araniy Perma Iskandar, Adhi Teguh Prayitno, Ari Karyanti, Mulya Rahma Satari, Hindra Irawan Jumiyanti, Niphidiah Aprilia, Yuni Yudha Sriyani, Ida Yus Dewi, Yora Permata Yudhaputri, Frilasita A Safari, Dodi Hadinegoro, Sri Rezeki Rosenberg, Ronald Powers, Ann M Aye Myint, Khin Saw |
description | Zika virus (ZIKV) has recently been confirmed as endemic in Indonesia, but no congenital anomalies (CA) related to ZIKV infection have been reported. We performed molecular and serological testing for ZIKV and other flaviviruses on cord serum and urine samples collected in October 2016 to April 2017 during a prospective, cross-sectional study of neonates in Jakarta, Indonesia. Of a total of 429 neonates, 53 had CA, including 14 with microcephaly. These 53, and 113 neonate controls without evidence of CA, were tested by ZIKV-specific real-time reverse transcription polymerase chain reaction (RT-PCR), pan-flavivirus RT-PCR, anti-ZIKV and anti-DENV IgM ELISA, and plaque reduction neutralization test. There was no evidence of ZIKV infection among neonates in either the CA or non-CA cohorts, except in three cases with low titers of anti-ZIKV neutralizing antibodies. Further routine evaluation throughout Indonesia of pregnant women and their newborns for exposure to ZIKV should be a high priority for determining risk. |
doi_str_mv | 10.4269/ajtmh.19-0593 |
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We performed molecular and serological testing for ZIKV and other flaviviruses on cord serum and urine samples collected in October 2016 to April 2017 during a prospective, cross-sectional study of neonates in Jakarta, Indonesia. Of a total of 429 neonates, 53 had CA, including 14 with microcephaly. These 53, and 113 neonate controls without evidence of CA, were tested by ZIKV-specific real-time reverse transcription polymerase chain reaction (RT-PCR), pan-flavivirus RT-PCR, anti-ZIKV and anti-DENV IgM ELISA, and plaque reduction neutralization test. There was no evidence of ZIKV infection among neonates in either the CA or non-CA cohorts, except in three cases with low titers of anti-ZIKV neutralizing antibodies. Further routine evaluation throughout Indonesia of pregnant women and their newborns for exposure to ZIKV should be a high priority for determining risk.</description><identifier>ISSN: 0002-9637</identifier><identifier>EISSN: 1476-1645</identifier><identifier>DOI: 10.4269/ajtmh.19-0593</identifier><identifier>PMID: 32043460</identifier><language>eng</language><publisher>United States: Institute of Tropical Medicine</publisher><subject>Adult ; Antibodies, Viral - blood ; Congenital Abnormalities - blood ; Congenital Abnormalities - etiology ; Congenital Abnormalities - urine ; Congenital Abnormalities - virology ; Congenital diseases ; Female ; Fetal Blood - virology ; Humans ; Immunoglobulin M - blood ; Immunoglobulin M - urine ; Indonesia - epidemiology ; Infant, Newborn ; Male ; Newborn babies ; Pregnancy ; Pregnancy Complications, Infectious - blood ; Pregnancy Complications, Infectious - urine ; Pregnancy Complications, Infectious - virology ; Serology ; Viral infections ; Young Adult ; Zika virus ; Zika Virus - isolation & purification ; Zika Virus Infection - blood ; Zika Virus Infection - urine ; Zika Virus Infection - virology</subject><ispartof>The American journal of tropical medicine and hygiene, 2020-04, Vol.102 (4), p.876-879</ispartof><rights>Copyright Institute of Tropical Medicine Apr 2020</rights><rights>The American Society of Tropical Medicine and Hygiene 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-ca855b9b6edab65405f8f34b74b6b7fdd26354c8e275dc7a52ece12da59425da3</citedby><cites>FETCH-LOGICAL-c415t-ca855b9b6edab65405f8f34b74b6b7fdd26354c8e275dc7a52ece12da59425da3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7124925/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7124925/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32043460$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Putri, Nina Dwi</creatorcontrib><creatorcontrib>Dhenni, Rama</creatorcontrib><creatorcontrib>Handryastuti, Setyo</creatorcontrib><creatorcontrib>Johar, Edison</creatorcontrib><creatorcontrib>Ma'roef, Chairin Nisa</creatorcontrib><creatorcontrib>Fadhilah, Araniy</creatorcontrib><creatorcontrib>Perma Iskandar, Adhi Teguh</creatorcontrib><creatorcontrib>Prayitno, Ari</creatorcontrib><creatorcontrib>Karyanti, Mulya Rahma</creatorcontrib><creatorcontrib>Satari, Hindra Irawan</creatorcontrib><creatorcontrib>Jumiyanti, Niphidiah</creatorcontrib><creatorcontrib>Aprilia, Yuni Yudha</creatorcontrib><creatorcontrib>Sriyani, Ida Yus</creatorcontrib><creatorcontrib>Dewi, Yora Permata</creatorcontrib><creatorcontrib>Yudhaputri, Frilasita A</creatorcontrib><creatorcontrib>Safari, Dodi</creatorcontrib><creatorcontrib>Hadinegoro, Sri Rezeki</creatorcontrib><creatorcontrib>Rosenberg, Ronald</creatorcontrib><creatorcontrib>Powers, Ann M</creatorcontrib><creatorcontrib>Aye Myint, Khin Saw</creatorcontrib><title>Absence of Evidence of Zika Virus Infection in Cord Blood and Urine from Newborns with Congenital Abnormalities, Indonesia</title><title>The American journal of tropical medicine and hygiene</title><addtitle>Am J Trop Med Hyg</addtitle><description>Zika virus (ZIKV) has recently been confirmed as endemic in Indonesia, but no congenital anomalies (CA) related to ZIKV infection have been reported. We performed molecular and serological testing for ZIKV and other flaviviruses on cord serum and urine samples collected in October 2016 to April 2017 during a prospective, cross-sectional study of neonates in Jakarta, Indonesia. Of a total of 429 neonates, 53 had CA, including 14 with microcephaly. These 53, and 113 neonate controls without evidence of CA, were tested by ZIKV-specific real-time reverse transcription polymerase chain reaction (RT-PCR), pan-flavivirus RT-PCR, anti-ZIKV and anti-DENV IgM ELISA, and plaque reduction neutralization test. There was no evidence of ZIKV infection among neonates in either the CA or non-CA cohorts, except in three cases with low titers of anti-ZIKV neutralizing antibodies. Further routine evaluation throughout Indonesia of pregnant women and their newborns for exposure to ZIKV should be a high priority for determining risk.</description><subject>Adult</subject><subject>Antibodies, Viral - blood</subject><subject>Congenital Abnormalities - blood</subject><subject>Congenital Abnormalities - etiology</subject><subject>Congenital Abnormalities - urine</subject><subject>Congenital Abnormalities - virology</subject><subject>Congenital diseases</subject><subject>Female</subject><subject>Fetal Blood - virology</subject><subject>Humans</subject><subject>Immunoglobulin M - blood</subject><subject>Immunoglobulin M - urine</subject><subject>Indonesia - epidemiology</subject><subject>Infant, Newborn</subject><subject>Male</subject><subject>Newborn babies</subject><subject>Pregnancy</subject><subject>Pregnancy Complications, Infectious - blood</subject><subject>Pregnancy Complications, Infectious - urine</subject><subject>Pregnancy Complications, Infectious - virology</subject><subject>Serology</subject><subject>Viral infections</subject><subject>Young Adult</subject><subject>Zika virus</subject><subject>Zika Virus - isolation & purification</subject><subject>Zika Virus Infection - blood</subject><subject>Zika Virus Infection - urine</subject><subject>Zika Virus Infection - virology</subject><issn>0002-9637</issn><issn>1476-1645</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkbtvFDEQhy0EIkegpEWWaCjYxO9dN0jHKZBIUWgIBY3lx2zOx64d7N1E8NezeQqoZqT59NPMfAi9puRAMKUP7W4atwdUN0Rq_gStqGhVQ5WQT9GKEMIarXi7h17UuiOEdozS52iPMyK4UGSFfq9dheQB5x4fXcXw0H-PPyz-Fstc8UnqwU8xJxwT3uQS8Mch54BtCvi8xAS4L3nEZ3DtckkVX8dpu3DpAlKc7IDXLuUy2iFOEer7JS7kBDXal-hZb4cKr-7rPjr_dPR1c9ycfvl8slmfNl5QOTXedlI67RQE65QURPZdz4VrhVOu7UNgikvhO2CtDL61koEHyoKVWjAZLN9HH-5yL2c3QvCQpmIHc1niaMsvk200_05S3JqLfGVayoRmcgl4dx9Q8s8Z6mTGWD0Mg02Q52oYl1x2nWR6Qd_-h-7yXNJynmFi-bnWi4OFau4oX3KtBfrHZSgxN1bNrVVDtbmxuvBv_r7gkX7QyP8ATjugTw</recordid><startdate>20200401</startdate><enddate>20200401</enddate><creator>Putri, Nina Dwi</creator><creator>Dhenni, Rama</creator><creator>Handryastuti, Setyo</creator><creator>Johar, Edison</creator><creator>Ma'roef, Chairin Nisa</creator><creator>Fadhilah, Araniy</creator><creator>Perma Iskandar, Adhi Teguh</creator><creator>Prayitno, Ari</creator><creator>Karyanti, Mulya Rahma</creator><creator>Satari, Hindra Irawan</creator><creator>Jumiyanti, Niphidiah</creator><creator>Aprilia, Yuni Yudha</creator><creator>Sriyani, Ida Yus</creator><creator>Dewi, Yora Permata</creator><creator>Yudhaputri, Frilasita A</creator><creator>Safari, Dodi</creator><creator>Hadinegoro, Sri Rezeki</creator><creator>Rosenberg, Ronald</creator><creator>Powers, Ann M</creator><creator>Aye Myint, Khin Saw</creator><general>Institute of Tropical Medicine</general><general>The American Society of Tropical Medicine and Hygiene</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20200401</creationdate><title>Absence of Evidence of Zika Virus Infection in Cord Blood and Urine from Newborns with Congenital Abnormalities, Indonesia</title><author>Putri, Nina Dwi ; 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We performed molecular and serological testing for ZIKV and other flaviviruses on cord serum and urine samples collected in October 2016 to April 2017 during a prospective, cross-sectional study of neonates in Jakarta, Indonesia. Of a total of 429 neonates, 53 had CA, including 14 with microcephaly. These 53, and 113 neonate controls without evidence of CA, were tested by ZIKV-specific real-time reverse transcription polymerase chain reaction (RT-PCR), pan-flavivirus RT-PCR, anti-ZIKV and anti-DENV IgM ELISA, and plaque reduction neutralization test. There was no evidence of ZIKV infection among neonates in either the CA or non-CA cohorts, except in three cases with low titers of anti-ZIKV neutralizing antibodies. Further routine evaluation throughout Indonesia of pregnant women and their newborns for exposure to ZIKV should be a high priority for determining risk.</abstract><cop>United States</cop><pub>Institute of Tropical Medicine</pub><pmid>32043460</pmid><doi>10.4269/ajtmh.19-0593</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Adult Antibodies, Viral - blood Congenital Abnormalities - blood Congenital Abnormalities - etiology Congenital Abnormalities - urine Congenital Abnormalities - virology Congenital diseases Female Fetal Blood - virology Humans Immunoglobulin M - blood Immunoglobulin M - urine Indonesia - epidemiology Infant, Newborn Male Newborn babies Pregnancy Pregnancy Complications, Infectious - blood Pregnancy Complications, Infectious - urine Pregnancy Complications, Infectious - virology Serology Viral infections Young Adult Zika virus Zika Virus - isolation & purification Zika Virus Infection - blood Zika Virus Infection - urine Zika Virus Infection - virology |
title | Absence of Evidence of Zika Virus Infection in Cord Blood and Urine from Newborns with Congenital Abnormalities, Indonesia |
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