Rapid detection and differentiation of dengue virus serotypes by NS1 specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay in patients presenting to a tertiary care hospital in Hyderabad, India

•This research article highlights the development of an improved, inexpensive, faster, sensitive and specific RT-LAMP assay for detection and serotyping of DENV, targeting NS1 region using Genie II fluorometer.•RT-LAMP or CDC Real time assay when used in combination with NS1 antigen and anti Dengue...

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Veröffentlicht in:	Journal of virological methods 2015-01, Vol.211, p.22-31
Hauptverfasser:	Neeraja, M., Lakshmi, V., Lavanya, Vanjari, Priyanka, E.N., Parida, M.M., Dash, P.K., Sharma, Shashi, Rao, P.V. Lakshmana, Reddy, Gopal
Format:	Artikel
Sprache:	eng
Schlagworte:	Dengue - diagnosis Dengue - virology Dengue Virus - classification Dengue Virus - genetics Dengue Virus - isolation & purification DENV serotypes Genie® II flourometer Humans India Molecular Sequence Data NS 1 antigen Nucleic Acid Amplification Techniques - methods Nucleic Acid Amplification Techniques - standards Reverse Transcription RNA, Viral - genetics RT-LAMP RT-PCR Sensitivity and Specificity Sequence Analysis, DNA Serogroup Serotyping Tertiary Care Centers Time Factors Viral Nonstructural Proteins - genetics
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