The bio in the ink: cartilage regeneration with bioprintable hydrogels and articular cartilage-derived progenitor cells
[Display omitted] Cell-laden hydrogels are the primary building blocks for bioprinting, and, also termed bioinks, are the foundations for creating structures that can potentially recapitulate the architecture of articular cartilage. To be functional, hydrogel constructs need to unlock the regenerati...
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| Veröffentlicht in: | Acta biomaterialia 2017-10, Vol.61, p.41-53 |
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| creator | Levato, Riccardo Webb, William R. Otto, Iris A. Mensinga, Anneloes Zhang, Yadan van Rijen, Mattie van Weeren, René Khan, Ilyas M. Malda, Jos |
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Cell-laden hydrogels are the primary building blocks for bioprinting, and, also termed bioinks, are the foundations for creating structures that can potentially recapitulate the architecture of articular cartilage. To be functional, hydrogel constructs need to unlock the regenerative capacity of encapsulated cells. The recent identification of multipotent articular cartilage-resident chondroprogenitor cells (ACPCs), which share important traits with adult stem cells, represents a new opportunity for cartilage regeneration. However, little is known about the suitability of ACPCs for tissue engineering, especially in combination with biomaterials. This study aimed to investigate the potential of ACPCs in hydrogels for cartilage regeneration and biofabrication, and to evaluate their ability for zone-specific matrix production. Gelatin methacryloyl (gelMA)-based hydrogels were used to culture ACPCs, bone marrow mesenchymal stromal cells (MSCs) and chondrocytes, and as bioinks for printing. Our data shows ACPCs outperformed chondrocytes in terms of neo-cartilage production and unlike MSCs, ACPCs had the lowest gene expression levels of hypertrophy marker collagen type X, and the highest expression of PRG4, a key factor in joint lubrication. Co-cultures of the cell types in multi-compartment hydrogels allowed generating constructs with a layered distribution of collagens and glycosaminoglycans. By combining ACPC- and MSC-laden bioinks, a bioprinted model of articular cartilage was generated, consisting of defined superficial and deep regions, each with distinct cellular and extracellular matrix composition. Taken together, these results provide important information for the use of ACPC-laden hydrogels in regenerative medicine, and pave the way to the biofabrication of 3D constructs with multiple cell types for cartilage regeneration or in vitro tissue models.
Despite its limited ability to repair, articular cartilage harbors an endogenous population of progenitor cells (ACPCs), that to date, received limited attention in biomaterials and tissue engineering applications. Harnessing the potential of these cells in 3D hydrogels can open new avenues for biomaterial-based regenerative therapies, especially with advanced biofabrication technologies (e.g. bioprinting). This study highlights the potential of ACPCs to generate neo-cartilage in a gelatin-based hydrogel and bioink. The ACPC-laden hydrogel is a suitable substrate for chondrogenesis and dat |
| doi_str_mv | 10.1016/j.actbio.2017.08.005 |
| format | Article |
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Cell-laden hydrogels are the primary building blocks for bioprinting, and, also termed bioinks, are the foundations for creating structures that can potentially recapitulate the architecture of articular cartilage. To be functional, hydrogel constructs need to unlock the regenerative capacity of encapsulated cells. The recent identification of multipotent articular cartilage-resident chondroprogenitor cells (ACPCs), which share important traits with adult stem cells, represents a new opportunity for cartilage regeneration. However, little is known about the suitability of ACPCs for tissue engineering, especially in combination with biomaterials. This study aimed to investigate the potential of ACPCs in hydrogels for cartilage regeneration and biofabrication, and to evaluate their ability for zone-specific matrix production. Gelatin methacryloyl (gelMA)-based hydrogels were used to culture ACPCs, bone marrow mesenchymal stromal cells (MSCs) and chondrocytes, and as bioinks for printing. Our data shows ACPCs outperformed chondrocytes in terms of neo-cartilage production and unlike MSCs, ACPCs had the lowest gene expression levels of hypertrophy marker collagen type X, and the highest expression of PRG4, a key factor in joint lubrication. Co-cultures of the cell types in multi-compartment hydrogels allowed generating constructs with a layered distribution of collagens and glycosaminoglycans. By combining ACPC- and MSC-laden bioinks, a bioprinted model of articular cartilage was generated, consisting of defined superficial and deep regions, each with distinct cellular and extracellular matrix composition. Taken together, these results provide important information for the use of ACPC-laden hydrogels in regenerative medicine, and pave the way to the biofabrication of 3D constructs with multiple cell types for cartilage regeneration or in vitro tissue models.
Despite its limited ability to repair, articular cartilage harbors an endogenous population of progenitor cells (ACPCs), that to date, received limited attention in biomaterials and tissue engineering applications. Harnessing the potential of these cells in 3D hydrogels can open new avenues for biomaterial-based regenerative therapies, especially with advanced biofabrication technologies (e.g. bioprinting). This study highlights the potential of ACPCs to generate neo-cartilage in a gelatin-based hydrogel and bioink. The ACPC-laden hydrogel is a suitable substrate for chondrogenesis and data shows it has a bias in directing cells towards a superficial zone phenotype. For the first time, ACPC-hydrogels are evaluated both as alternative for and in combination with chondrocytes and MSCs, using co-cultures and bioprinting for cartilage regeneration in vitro. This study provides important cues on ACPCs, indicating they represent a promising cell source for the next generation of cartilage constructs with increased biomimicry.</description><identifier>ISSN: 1742-7061</identifier><identifier>EISSN: 1878-7568</identifier><identifier>DOI: 10.1016/j.actbio.2017.08.005</identifier><identifier>PMID: 28782725</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Bioengineering ; Biofabrication ; Biomarkers - metabolism ; Biomaterials ; Biomedical materials ; Bioprinting - methods ; Bone marrow ; Cartilage ; Cartilage (articular) ; Cartilage regeneration ; Cartilage, Articular - cytology ; Cell culture ; Cell Differentiation - drug effects ; Cell Differentiation - genetics ; Cells (biology) ; Cells, Cultured ; Chondrocytes ; Chondrogenesis - drug effects ; Chondrogenesis - genetics ; Chondroprogenitor cells ; Co-culture ; Coculture Techniques ; Collagen ; Compressive Strength ; DNA - metabolism ; Extracellular matrix ; Gelatin ; Gene expression ; Glycosaminoglycans ; Glycosaminoglycans - metabolism ; Horses ; Hydrogel ; Hydrogel, Polyethylene Glycol Dimethacrylate - pharmacology ; Hydrogels ; Hydrogels - pharmacology ; Hypertrophy ; Ink ; Lubrication ; Mesenchymal Stem Cells - cytology ; Mesenchymal Stem Cells - drug effects ; Mesenchymal Stem Cells - metabolism ; Mesenchyme ; Printing ; Regeneration (physiology) ; Regeneration - drug effects ; Regenerative medicine ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Stem cells ; Stem Cells - cytology ; Stem Cells - drug effects ; Stromal cells ; Sus scrofa ; Three dimensional printing ; Tissue engineering</subject><ispartof>Acta biomaterialia, 2017-10, Vol.61, p.41-53</ispartof><rights>2017 Acta Materialia Inc.</rights><rights>Copyright © 2017 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier BV Oct 1, 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c600t-be7685648166349da759705427ad3271cc70af32c13601995992cf7e3c27cfab3</citedby><cites>FETCH-LOGICAL-c600t-be7685648166349da759705427ad3271cc70af32c13601995992cf7e3c27cfab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.actbio.2017.08.005$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28782725$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Levato, Riccardo</creatorcontrib><creatorcontrib>Webb, William R.</creatorcontrib><creatorcontrib>Otto, Iris A.</creatorcontrib><creatorcontrib>Mensinga, Anneloes</creatorcontrib><creatorcontrib>Zhang, Yadan</creatorcontrib><creatorcontrib>van Rijen, Mattie</creatorcontrib><creatorcontrib>van Weeren, René</creatorcontrib><creatorcontrib>Khan, Ilyas M.</creatorcontrib><creatorcontrib>Malda, Jos</creatorcontrib><title>The bio in the ink: cartilage regeneration with bioprintable hydrogels and articular cartilage-derived progenitor cells</title><title>Acta biomaterialia</title><addtitle>Acta Biomater</addtitle><description>[Display omitted]
Cell-laden hydrogels are the primary building blocks for bioprinting, and, also termed bioinks, are the foundations for creating structures that can potentially recapitulate the architecture of articular cartilage. To be functional, hydrogel constructs need to unlock the regenerative capacity of encapsulated cells. The recent identification of multipotent articular cartilage-resident chondroprogenitor cells (ACPCs), which share important traits with adult stem cells, represents a new opportunity for cartilage regeneration. However, little is known about the suitability of ACPCs for tissue engineering, especially in combination with biomaterials. This study aimed to investigate the potential of ACPCs in hydrogels for cartilage regeneration and biofabrication, and to evaluate their ability for zone-specific matrix production. Gelatin methacryloyl (gelMA)-based hydrogels were used to culture ACPCs, bone marrow mesenchymal stromal cells (MSCs) and chondrocytes, and as bioinks for printing. Our data shows ACPCs outperformed chondrocytes in terms of neo-cartilage production and unlike MSCs, ACPCs had the lowest gene expression levels of hypertrophy marker collagen type X, and the highest expression of PRG4, a key factor in joint lubrication. Co-cultures of the cell types in multi-compartment hydrogels allowed generating constructs with a layered distribution of collagens and glycosaminoglycans. By combining ACPC- and MSC-laden bioinks, a bioprinted model of articular cartilage was generated, consisting of defined superficial and deep regions, each with distinct cellular and extracellular matrix composition. Taken together, these results provide important information for the use of ACPC-laden hydrogels in regenerative medicine, and pave the way to the biofabrication of 3D constructs with multiple cell types for cartilage regeneration or in vitro tissue models.
Despite its limited ability to repair, articular cartilage harbors an endogenous population of progenitor cells (ACPCs), that to date, received limited attention in biomaterials and tissue engineering applications. Harnessing the potential of these cells in 3D hydrogels can open new avenues for biomaterial-based regenerative therapies, especially with advanced biofabrication technologies (e.g. bioprinting). This study highlights the potential of ACPCs to generate neo-cartilage in a gelatin-based hydrogel and bioink. The ACPC-laden hydrogel is a suitable substrate for chondrogenesis and data shows it has a bias in directing cells towards a superficial zone phenotype. For the first time, ACPC-hydrogels are evaluated both as alternative for and in combination with chondrocytes and MSCs, using co-cultures and bioprinting for cartilage regeneration in vitro. This study provides important cues on ACPCs, indicating they represent a promising cell source for the next generation of cartilage constructs with increased biomimicry.</description><subject>Animals</subject><subject>Bioengineering</subject><subject>Biofabrication</subject><subject>Biomarkers - metabolism</subject><subject>Biomaterials</subject><subject>Biomedical materials</subject><subject>Bioprinting - methods</subject><subject>Bone marrow</subject><subject>Cartilage</subject><subject>Cartilage (articular)</subject><subject>Cartilage regeneration</subject><subject>Cartilage, Articular - cytology</subject><subject>Cell culture</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Differentiation - genetics</subject><subject>Cells (biology)</subject><subject>Cells, Cultured</subject><subject>Chondrocytes</subject><subject>Chondrogenesis - drug effects</subject><subject>Chondrogenesis - genetics</subject><subject>Chondroprogenitor cells</subject><subject>Co-culture</subject><subject>Coculture Techniques</subject><subject>Collagen</subject><subject>Compressive Strength</subject><subject>DNA - metabolism</subject><subject>Extracellular matrix</subject><subject>Gelatin</subject><subject>Gene expression</subject><subject>Glycosaminoglycans</subject><subject>Glycosaminoglycans - metabolism</subject><subject>Horses</subject><subject>Hydrogel</subject><subject>Hydrogel, Polyethylene Glycol Dimethacrylate - pharmacology</subject><subject>Hydrogels</subject><subject>Hydrogels - pharmacology</subject><subject>Hypertrophy</subject><subject>Ink</subject><subject>Lubrication</subject><subject>Mesenchymal Stem Cells - cytology</subject><subject>Mesenchymal Stem Cells - drug effects</subject><subject>Mesenchymal Stem Cells - metabolism</subject><subject>Mesenchyme</subject><subject>Printing</subject><subject>Regeneration (physiology)</subject><subject>Regeneration - drug effects</subject><subject>Regenerative medicine</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Stem cells</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - drug effects</subject><subject>Stromal cells</subject><subject>Sus scrofa</subject><subject>Three dimensional printing</subject><subject>Tissue engineering</subject><issn>1742-7061</issn><issn>1878-7568</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtv1DAUhS0EoqXwDxCyxIZNgh-JHbNAQhUvqRKbsrYc-2bGQ8YebGeq_nscTWmBBStf6X7n-B4dhF5S0lJCxdtda2wZfWwZobIlQ0tI_wid00EOjezF8LjOsmONJIKeoWc57wjhA2XDU3TGKsQk68_RzfUWcHXBPuBSRx9-vMPWpOJnswGcYAMBkik-Bnzjy3ZlD8mHYsYZ8PbWpbiBOWMTHF5VdplNejBoHCR_BIcPKxd8iXUJ85yfoyeTmTO8uHsv0PdPH68vvzRX3z5_vfxw1VhBSGlGkGLoRTdQIXinnJG9kqTvmDSOM0mtlcRMnFnKBaFK9UoxO0nglkk7mZFfoPcn38My7sFZCCWZWdcIe5NudTRe_70Jfqs38aglpYIwXg3e3Bmk-HOBXPTe5zWCCRCXrKliQklFharo63_QXVxSqPEqJbqeKk5WqjtRNsWcE0z3x1Ci12b1Tp-a1Wuzmgy6Nltlr_4Mci_6XeVD0loHHD0kna2HYMH5BLZoF_3_f_gFrSy4gw</recordid><startdate>20171001</startdate><enddate>20171001</enddate><creator>Levato, Riccardo</creator><creator>Webb, William R.</creator><creator>Otto, Iris A.</creator><creator>Mensinga, Anneloes</creator><creator>Zhang, Yadan</creator><creator>van Rijen, Mattie</creator><creator>van Weeren, René</creator><creator>Khan, Ilyas M.</creator><creator>Malda, Jos</creator><general>Elsevier Ltd</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20171001</creationdate><title>The bio in the ink: cartilage regeneration with bioprintable hydrogels and articular cartilage-derived progenitor cells</title><author>Levato, Riccardo ; Webb, William R. ; Otto, Iris A. ; Mensinga, Anneloes ; Zhang, Yadan ; van Rijen, Mattie ; van Weeren, René ; Khan, Ilyas M. ; Malda, Jos</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c600t-be7685648166349da759705427ad3271cc70af32c13601995992cf7e3c27cfab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Bioengineering</topic><topic>Biofabrication</topic><topic>Biomarkers - metabolism</topic><topic>Biomaterials</topic><topic>Biomedical materials</topic><topic>Bioprinting - methods</topic><topic>Bone marrow</topic><topic>Cartilage</topic><topic>Cartilage (articular)</topic><topic>Cartilage regeneration</topic><topic>Cartilage, Articular - cytology</topic><topic>Cell culture</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Differentiation - genetics</topic><topic>Cells (biology)</topic><topic>Cells, Cultured</topic><topic>Chondrocytes</topic><topic>Chondrogenesis - drug effects</topic><topic>Chondrogenesis - genetics</topic><topic>Chondroprogenitor cells</topic><topic>Co-culture</topic><topic>Coculture Techniques</topic><topic>Collagen</topic><topic>Compressive Strength</topic><topic>DNA - metabolism</topic><topic>Extracellular matrix</topic><topic>Gelatin</topic><topic>Gene expression</topic><topic>Glycosaminoglycans</topic><topic>Glycosaminoglycans - metabolism</topic><topic>Horses</topic><topic>Hydrogel</topic><topic>Hydrogel, Polyethylene Glycol Dimethacrylate - pharmacology</topic><topic>Hydrogels</topic><topic>Hydrogels - pharmacology</topic><topic>Hypertrophy</topic><topic>Ink</topic><topic>Lubrication</topic><topic>Mesenchymal Stem Cells - cytology</topic><topic>Mesenchymal Stem Cells - drug effects</topic><topic>Mesenchymal Stem Cells - metabolism</topic><topic>Mesenchyme</topic><topic>Printing</topic><topic>Regeneration (physiology)</topic><topic>Regeneration - drug effects</topic><topic>Regenerative medicine</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Stem cells</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - drug effects</topic><topic>Stromal cells</topic><topic>Sus scrofa</topic><topic>Three dimensional printing</topic><topic>Tissue engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Levato, Riccardo</creatorcontrib><creatorcontrib>Webb, William R.</creatorcontrib><creatorcontrib>Otto, Iris A.</creatorcontrib><creatorcontrib>Mensinga, Anneloes</creatorcontrib><creatorcontrib>Zhang, Yadan</creatorcontrib><creatorcontrib>van Rijen, Mattie</creatorcontrib><creatorcontrib>van Weeren, René</creatorcontrib><creatorcontrib>Khan, Ilyas M.</creatorcontrib><creatorcontrib>Malda, Jos</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Acta biomaterialia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Levato, Riccardo</au><au>Webb, William R.</au><au>Otto, Iris A.</au><au>Mensinga, Anneloes</au><au>Zhang, Yadan</au><au>van Rijen, Mattie</au><au>van Weeren, René</au><au>Khan, Ilyas M.</au><au>Malda, Jos</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The bio in the ink: cartilage regeneration with bioprintable hydrogels and articular cartilage-derived progenitor cells</atitle><jtitle>Acta biomaterialia</jtitle><addtitle>Acta Biomater</addtitle><date>2017-10-01</date><risdate>2017</risdate><volume>61</volume><spage>41</spage><epage>53</epage><pages>41-53</pages><issn>1742-7061</issn><eissn>1878-7568</eissn><abstract>[Display omitted]
Cell-laden hydrogels are the primary building blocks for bioprinting, and, also termed bioinks, are the foundations for creating structures that can potentially recapitulate the architecture of articular cartilage. To be functional, hydrogel constructs need to unlock the regenerative capacity of encapsulated cells. The recent identification of multipotent articular cartilage-resident chondroprogenitor cells (ACPCs), which share important traits with adult stem cells, represents a new opportunity for cartilage regeneration. However, little is known about the suitability of ACPCs for tissue engineering, especially in combination with biomaterials. This study aimed to investigate the potential of ACPCs in hydrogels for cartilage regeneration and biofabrication, and to evaluate their ability for zone-specific matrix production. Gelatin methacryloyl (gelMA)-based hydrogels were used to culture ACPCs, bone marrow mesenchymal stromal cells (MSCs) and chondrocytes, and as bioinks for printing. Our data shows ACPCs outperformed chondrocytes in terms of neo-cartilage production and unlike MSCs, ACPCs had the lowest gene expression levels of hypertrophy marker collagen type X, and the highest expression of PRG4, a key factor in joint lubrication. Co-cultures of the cell types in multi-compartment hydrogels allowed generating constructs with a layered distribution of collagens and glycosaminoglycans. By combining ACPC- and MSC-laden bioinks, a bioprinted model of articular cartilage was generated, consisting of defined superficial and deep regions, each with distinct cellular and extracellular matrix composition. Taken together, these results provide important information for the use of ACPC-laden hydrogels in regenerative medicine, and pave the way to the biofabrication of 3D constructs with multiple cell types for cartilage regeneration or in vitro tissue models.
Despite its limited ability to repair, articular cartilage harbors an endogenous population of progenitor cells (ACPCs), that to date, received limited attention in biomaterials and tissue engineering applications. Harnessing the potential of these cells in 3D hydrogels can open new avenues for biomaterial-based regenerative therapies, especially with advanced biofabrication technologies (e.g. bioprinting). This study highlights the potential of ACPCs to generate neo-cartilage in a gelatin-based hydrogel and bioink. The ACPC-laden hydrogel is a suitable substrate for chondrogenesis and data shows it has a bias in directing cells towards a superficial zone phenotype. For the first time, ACPC-hydrogels are evaluated both as alternative for and in combination with chondrocytes and MSCs, using co-cultures and bioprinting for cartilage regeneration in vitro. This study provides important cues on ACPCs, indicating they represent a promising cell source for the next generation of cartilage constructs with increased biomimicry.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>28782725</pmid><doi>10.1016/j.actbio.2017.08.005</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1742-7061 |
| ispartof | Acta biomaterialia, 2017-10, Vol.61, p.41-53 |
| issn | 1742-7061 1878-7568 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7116023 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Bioengineering Biofabrication Biomarkers - metabolism Biomaterials Biomedical materials Bioprinting - methods Bone marrow Cartilage Cartilage (articular) Cartilage regeneration Cartilage, Articular - cytology Cell culture Cell Differentiation - drug effects Cell Differentiation - genetics Cells (biology) Cells, Cultured Chondrocytes Chondrogenesis - drug effects Chondrogenesis - genetics Chondroprogenitor cells Co-culture Coculture Techniques Collagen Compressive Strength DNA - metabolism Extracellular matrix Gelatin Gene expression Glycosaminoglycans Glycosaminoglycans - metabolism Horses Hydrogel Hydrogel, Polyethylene Glycol Dimethacrylate - pharmacology Hydrogels Hydrogels - pharmacology Hypertrophy Ink Lubrication Mesenchymal Stem Cells - cytology Mesenchymal Stem Cells - drug effects Mesenchymal Stem Cells - metabolism Mesenchyme Printing Regeneration (physiology) Regeneration - drug effects Regenerative medicine RNA, Messenger - genetics RNA, Messenger - metabolism Stem cells Stem Cells - cytology Stem Cells - drug effects Stromal cells Sus scrofa Three dimensional printing Tissue engineering |
| title | The bio in the ink: cartilage regeneration with bioprintable hydrogels and articular cartilage-derived progenitor cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T02%3A05%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20bio%20in%20the%20ink:%20cartilage%20regeneration%20with%20bioprintable%20hydrogels%20and%20articular%20cartilage-derived%20progenitor%20cells&rft.jtitle=Acta%20biomaterialia&rft.au=Levato,%20Riccardo&rft.date=2017-10-01&rft.volume=61&rft.spage=41&rft.epage=53&rft.pages=41-53&rft.issn=1742-7061&rft.eissn=1878-7568&rft_id=info:doi/10.1016/j.actbio.2017.08.005&rft_dat=%3Cproquest_pubme%3E1926979169%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1964519309&rft_id=info:pmid/28782725&rft_els_id=S1742706117304956&rfr_iscdi=true |