Expression, Purification, Crystallization, and Enzyme Assays of Fumarylacetoacetate Hydrolase Domain-Containing Proteins

Fumarylacetoacetate hydrolase (FAH) domain-containing proteins (FAHD) are identified members of the FAH superfamily in eukaryotes. Enzymes of this superfamily generally display multi-functionality, involving mainly hydrolase and decarboxylase mechanisms. This article presents a series of consecutive...

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Veröffentlicht in:	Journal of visualized experiments 2019-06 (148)
Hauptverfasser:	Weiss, Alexander K H, Holzknecht, Max, Cappuccio, Elia, Dorigatti, Ilaria, Kreidl, Karin, Naschberger, Andreas, Rupp, Bernhard, Gstach, Hubert, Jansen-Dürr, Pidder
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography, Liquid - methods Crystallization Crystallography, X-Ray Enzyme Assays - methods Escherichia coli - metabolism Hydrolases - chemistry Hydrolases - isolation & purification Hydrolases - metabolism Protein Domains
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