rOv-ASP-1, a recombinant secreted protein of the helminth Onchocercavolvulus, is a potent adjuvant for inducing antibodies to ovalbumin, HIV-1 polypeptide and SARS-CoV peptide antigens
We studied the adjuvanticity of recombinant Onchocerca volvulus activation associated protein-1 (rOv-ASP-1) for ovalbumin (OVA) in mice. After a single immunization and one boost, rOv-ASP-1 exceeded the efficacy of alum or MPL + TDM adjuvants in terms of end-point total IgG or IgG1 and IgG2a anti-OV...
Gespeichert in:
| Veröffentlicht in: | Vaccine 2005-05, Vol.23 (26), p.3446-3452 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 3452 |
|---|---|
| container_issue | 26 |
| container_start_page | 3446 |
| container_title | Vaccine |
| container_volume | 23 |
| creator | MacDonald, Angus J Cao, Long He, Yuxian Zhao, Qian Jiang, Shibo Lustigman, Sara |
| description | We studied the adjuvanticity of recombinant Onchocerca volvulus activation associated protein-1 (rOv-ASP-1) for ovalbumin (OVA) in mice. After a single immunization and one boost, rOv-ASP-1 exceeded the efficacy of alum or MPL + TDM adjuvants in terms of end-point total IgG or IgG1 and IgG2a anti-OVA titres. Using the helminth-derived adjuvant, IgG isotype responses to OVA were of a mixed Th1/Th2 profile and spleen cell cytokines exclusively Th1-type. The potent adjuvanticity of rOv-ASP-1 was confirmed in mice vaccinated with a 37-mer peptide from the S protein of SARS-CoV and an HIV-1 gp120-CD4 chimeric polypeptide antigen. Unusually for a helminth product, the rOv-ASP-1 adjuvant augmented not only Th2 but also Th1 responses, the latter property being of potential utility in stimulating anti-viral immune responses. |
| doi_str_mv | 10.1016/j.vaccine.2005.01.098 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7115491</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>67758447</sourcerecordid><originalsourceid>FETCH-LOGICAL-p1091-ab357730e12a8d6a66a39b28b18a25f3d6ddeac12bd8a16900a056e432aca02a3</originalsourceid><addsrcrecordid>eNpVkd1u1DAQhS0EotvCI4B8xdUm2HHiODdIqxXQSpUWsVBxF03s2Y1XWTvYSaS-GY-HK8rf1UhnznxnRkPIK85yzrh8e8oX0No6zAvGqpzxnDXqCVlxVYusqLh6SlaskGVWcvbtglzGeGLJKHjznFzwSolaSLUiP8JuyTb7TxlfU6ABtT931oGbaEQdcEJDx-AntI76A516pD0OZ-umnu6c7r3GoGHxwzIPc1xTGxNlTP4EAHOalwfSwQdqnZnTtkeaBNt5YzHSyVO_wNDNibem1zd3GU-zw_2I42QNJquh-83nfbb1d_SvONkjuviCPDvAEPHlY70iXz-8_7K9zm53H2-2m9ts5KzhGXSiqmvBkBegjAQpQTRdoTquoKgOwkhjEDQvOqOAy4YxYJXEUhSggRUgrsi7X9xx7s5odLoswNCOwZ4h3LcebPt_x9m-PfqlrTmvyoYnwJtHQPDfZ4xTe7ZR4zCAQz_HVtZ1pcqyTsbX_yb9ifj9LPETVeqdXg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>67758447</pqid></control><display><type>article</type><title>rOv-ASP-1, a recombinant secreted protein of the helminth Onchocercavolvulus, is a potent adjuvant for inducing antibodies to ovalbumin, HIV-1 polypeptide and SARS-CoV peptide antigens</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><source>ProQuest Central UK/Ireland</source><creator>MacDonald, Angus J ; Cao, Long ; He, Yuxian ; Zhao, Qian ; Jiang, Shibo ; Lustigman, Sara</creator><creatorcontrib>MacDonald, Angus J ; Cao, Long ; He, Yuxian ; Zhao, Qian ; Jiang, Shibo ; Lustigman, Sara</creatorcontrib><description>We studied the adjuvanticity of recombinant Onchocerca volvulus activation associated protein-1 (rOv-ASP-1) for ovalbumin (OVA) in mice. After a single immunization and one boost, rOv-ASP-1 exceeded the efficacy of alum or MPL + TDM adjuvants in terms of end-point total IgG or IgG1 and IgG2a anti-OVA titres. Using the helminth-derived adjuvant, IgG isotype responses to OVA were of a mixed Th1/Th2 profile and spleen cell cytokines exclusively Th1-type. The potent adjuvanticity of rOv-ASP-1 was confirmed in mice vaccinated with a 37-mer peptide from the S protein of SARS-CoV and an HIV-1 gp120-CD4 chimeric polypeptide antigen. Unusually for a helminth product, the rOv-ASP-1 adjuvant augmented not only Th2 but also Th1 responses, the latter property being of potential utility in stimulating anti-viral immune responses.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2005.01.098</identifier><identifier>PMID: 15837368</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Adjuvants, Immunologic - pharmacology ; Animals ; Antigens, Helminth - administration & dosage ; Antigens, Helminth - genetics ; Antigens, Helminth - immunology ; Antigens, Helminth - pharmacology ; Helminth Proteins - pharmacology ; HIV-1 - drug effects ; HIV-1 - immunology ; Mice ; Ovalbumin - chemistry ; Ovalbumin - immunology ; Recombinant Proteins - immunology ; Recombinant Proteins - pharmacology ; SARS Virus - drug effects ; SARS Virus - immunology ; Secretory Vesicles - metabolism ; Th1 Cells - immunology ; Th2 Cells - immunology ; Vaccines, Synthetic - administration & dosage ; Vaccines, Synthetic - immunology</subject><ispartof>Vaccine, 2005-05, Vol.23 (26), p.3446-3452</ispartof><rights>Copyright © 2005 Elsevier Ltd. All rights reserved. 2005 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925,64387</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15837368$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MacDonald, Angus J</creatorcontrib><creatorcontrib>Cao, Long</creatorcontrib><creatorcontrib>He, Yuxian</creatorcontrib><creatorcontrib>Zhao, Qian</creatorcontrib><creatorcontrib>Jiang, Shibo</creatorcontrib><creatorcontrib>Lustigman, Sara</creatorcontrib><title>rOv-ASP-1, a recombinant secreted protein of the helminth Onchocercavolvulus, is a potent adjuvant for inducing antibodies to ovalbumin, HIV-1 polypeptide and SARS-CoV peptide antigens</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>We studied the adjuvanticity of recombinant Onchocerca volvulus activation associated protein-1 (rOv-ASP-1) for ovalbumin (OVA) in mice. After a single immunization and one boost, rOv-ASP-1 exceeded the efficacy of alum or MPL + TDM adjuvants in terms of end-point total IgG or IgG1 and IgG2a anti-OVA titres. Using the helminth-derived adjuvant, IgG isotype responses to OVA were of a mixed Th1/Th2 profile and spleen cell cytokines exclusively Th1-type. The potent adjuvanticity of rOv-ASP-1 was confirmed in mice vaccinated with a 37-mer peptide from the S protein of SARS-CoV and an HIV-1 gp120-CD4 chimeric polypeptide antigen. Unusually for a helminth product, the rOv-ASP-1 adjuvant augmented not only Th2 but also Th1 responses, the latter property being of potential utility in stimulating anti-viral immune responses.</description><subject>Adjuvants, Immunologic - pharmacology</subject><subject>Animals</subject><subject>Antigens, Helminth - administration & dosage</subject><subject>Antigens, Helminth - genetics</subject><subject>Antigens, Helminth - immunology</subject><subject>Antigens, Helminth - pharmacology</subject><subject>Helminth Proteins - pharmacology</subject><subject>HIV-1 - drug effects</subject><subject>HIV-1 - immunology</subject><subject>Mice</subject><subject>Ovalbumin - chemistry</subject><subject>Ovalbumin - immunology</subject><subject>Recombinant Proteins - immunology</subject><subject>Recombinant Proteins - pharmacology</subject><subject>SARS Virus - drug effects</subject><subject>SARS Virus - immunology</subject><subject>Secretory Vesicles - metabolism</subject><subject>Th1 Cells - immunology</subject><subject>Th2 Cells - immunology</subject><subject>Vaccines, Synthetic - administration & dosage</subject><subject>Vaccines, Synthetic - immunology</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkd1u1DAQhS0EotvCI4B8xdUm2HHiODdIqxXQSpUWsVBxF03s2Y1XWTvYSaS-GY-HK8rf1UhnznxnRkPIK85yzrh8e8oX0No6zAvGqpzxnDXqCVlxVYusqLh6SlaskGVWcvbtglzGeGLJKHjznFzwSolaSLUiP8JuyTb7TxlfU6ABtT931oGbaEQdcEJDx-AntI76A516pD0OZ-umnu6c7r3GoGHxwzIPc1xTGxNlTP4EAHOalwfSwQdqnZnTtkeaBNt5YzHSyVO_wNDNibem1zd3GU-zw_2I42QNJquh-83nfbb1d_SvONkjuviCPDvAEPHlY70iXz-8_7K9zm53H2-2m9ts5KzhGXSiqmvBkBegjAQpQTRdoTquoKgOwkhjEDQvOqOAy4YxYJXEUhSggRUgrsi7X9xx7s5odLoswNCOwZ4h3LcebPt_x9m-PfqlrTmvyoYnwJtHQPDfZ4xTe7ZR4zCAQz_HVtZ1pcqyTsbX_yb9ifj9LPETVeqdXg</recordid><startdate>20050516</startdate><enddate>20050516</enddate><creator>MacDonald, Angus J</creator><creator>Cao, Long</creator><creator>He, Yuxian</creator><creator>Zhao, Qian</creator><creator>Jiang, Shibo</creator><creator>Lustigman, Sara</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20050516</creationdate><title>rOv-ASP-1, a recombinant secreted protein of the helminth Onchocercavolvulus, is a potent adjuvant for inducing antibodies to ovalbumin, HIV-1 polypeptide and SARS-CoV peptide antigens</title><author>MacDonald, Angus J ; Cao, Long ; He, Yuxian ; Zhao, Qian ; Jiang, Shibo ; Lustigman, Sara</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p1091-ab357730e12a8d6a66a39b28b18a25f3d6ddeac12bd8a16900a056e432aca02a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adjuvants, Immunologic - pharmacology</topic><topic>Animals</topic><topic>Antigens, Helminth - administration & dosage</topic><topic>Antigens, Helminth - genetics</topic><topic>Antigens, Helminth - immunology</topic><topic>Antigens, Helminth - pharmacology</topic><topic>Helminth Proteins - pharmacology</topic><topic>HIV-1 - drug effects</topic><topic>HIV-1 - immunology</topic><topic>Mice</topic><topic>Ovalbumin - chemistry</topic><topic>Ovalbumin - immunology</topic><topic>Recombinant Proteins - immunology</topic><topic>Recombinant Proteins - pharmacology</topic><topic>SARS Virus - drug effects</topic><topic>SARS Virus - immunology</topic><topic>Secretory Vesicles - metabolism</topic><topic>Th1 Cells - immunology</topic><topic>Th2 Cells - immunology</topic><topic>Vaccines, Synthetic - administration & dosage</topic><topic>Vaccines, Synthetic - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MacDonald, Angus J</creatorcontrib><creatorcontrib>Cao, Long</creatorcontrib><creatorcontrib>He, Yuxian</creatorcontrib><creatorcontrib>Zhao, Qian</creatorcontrib><creatorcontrib>Jiang, Shibo</creatorcontrib><creatorcontrib>Lustigman, Sara</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MacDonald, Angus J</au><au>Cao, Long</au><au>He, Yuxian</au><au>Zhao, Qian</au><au>Jiang, Shibo</au><au>Lustigman, Sara</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>rOv-ASP-1, a recombinant secreted protein of the helminth Onchocercavolvulus, is a potent adjuvant for inducing antibodies to ovalbumin, HIV-1 polypeptide and SARS-CoV peptide antigens</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2005-05-16</date><risdate>2005</risdate><volume>23</volume><issue>26</issue><spage>3446</spage><epage>3452</epage><pages>3446-3452</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><abstract>We studied the adjuvanticity of recombinant Onchocerca volvulus activation associated protein-1 (rOv-ASP-1) for ovalbumin (OVA) in mice. After a single immunization and one boost, rOv-ASP-1 exceeded the efficacy of alum or MPL + TDM adjuvants in terms of end-point total IgG or IgG1 and IgG2a anti-OVA titres. Using the helminth-derived adjuvant, IgG isotype responses to OVA were of a mixed Th1/Th2 profile and spleen cell cytokines exclusively Th1-type. The potent adjuvanticity of rOv-ASP-1 was confirmed in mice vaccinated with a 37-mer peptide from the S protein of SARS-CoV and an HIV-1 gp120-CD4 chimeric polypeptide antigen. Unusually for a helminth product, the rOv-ASP-1 adjuvant augmented not only Th2 but also Th1 responses, the latter property being of potential utility in stimulating anti-viral immune responses.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>15837368</pmid><doi>10.1016/j.vaccine.2005.01.098</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0264-410X |
| ispartof | Vaccine, 2005-05, Vol.23 (26), p.3446-3452 |
| issn | 0264-410X 1873-2518 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7115491 |
| source | MEDLINE; Access via ScienceDirect (Elsevier); ProQuest Central UK/Ireland |
| subjects | Adjuvants, Immunologic - pharmacology Animals Antigens, Helminth - administration & dosage Antigens, Helminth - genetics Antigens, Helminth - immunology Antigens, Helminth - pharmacology Helminth Proteins - pharmacology HIV-1 - drug effects HIV-1 - immunology Mice Ovalbumin - chemistry Ovalbumin - immunology Recombinant Proteins - immunology Recombinant Proteins - pharmacology SARS Virus - drug effects SARS Virus - immunology Secretory Vesicles - metabolism Th1 Cells - immunology Th2 Cells - immunology Vaccines, Synthetic - administration & dosage Vaccines, Synthetic - immunology |
| title | rOv-ASP-1, a recombinant secreted protein of the helminth Onchocercavolvulus, is a potent adjuvant for inducing antibodies to ovalbumin, HIV-1 polypeptide and SARS-CoV peptide antigens |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T01%3A35%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=rOv-ASP-1,%20a%20recombinant%20secreted%20protein%20of%20the%20helminth%20Onchocercavolvulus,%20is%20a%20potent%20adjuvant%20for%20inducing%20antibodies%20to%20ovalbumin,%20HIV-1%20polypeptide%20and%20SARS-CoV%20peptide%20antigens&rft.jtitle=Vaccine&rft.au=MacDonald,%20Angus%20J&rft.date=2005-05-16&rft.volume=23&rft.issue=26&rft.spage=3446&rft.epage=3452&rft.pages=3446-3452&rft.issn=0264-410X&rft.eissn=1873-2518&rft_id=info:doi/10.1016/j.vaccine.2005.01.098&rft_dat=%3Cproquest_pubme%3E67758447%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=67758447&rft_id=info:pmid/15837368&rfr_iscdi=true |