Mutation of neutralizing/antibody-dependent enhancing epitope on spike protein and 7b gene of feline infectious peritonitis virus: Influences of viral replication in monocytes/macrophages and virulence in cats
We previously prepared neutralizing monoclonal antibody (MAb)-resistant (mar) mutant viruses using a laboratory strain feline infectious peritonitis virus (FIPV) 79-1146 (Kida et al., 1999). Mar mutant viruses are mutated several amino acids of the neutralizing epitope of Spike protein, compared wit...
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| Veröffentlicht in: | Virus research 2011-03, Vol.156 (1-2), p.72-80 |
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| creator | Takano, Tomomi Tomiyama, Yoshika Katoh, Yasuichiroh Nakamura, Michiyo Satoh, Ryoichi Hohdatsu, Tsutomu |
| description | We previously prepared neutralizing monoclonal antibody (MAb)-resistant (mar) mutant viruses using a laboratory strain feline infectious peritonitis virus (FIPV) 79-1146 (Kida et al., 1999). Mar mutant viruses are mutated several amino acids of the neutralizing epitope of Spike protein, compared with the parent strain, FIPV 79-1146. We clarified that MAb used to prepare mar mutant viruses also lost its activity to enhance homologous mar mutant viruses, strongly suggesting that neutralizing and antibody-dependent enhancing epitopes are present in the same region in the strain FIPV 79-1146. We also discovered that amino acid mutation in the neutralizing epitope reduced viral replication in monocytes/macrophages. We also demonstrated that the mutation or deletion of two nucleotides in 7b gene abrogate the virulence of strain FIPV 79-1146. |
| doi_str_mv | 10.1016/j.virusres.2010.12.020 |
| format | Article |
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Mar mutant viruses are mutated several amino acids of the neutralizing epitope of Spike protein, compared with the parent strain, FIPV 79-1146. We clarified that MAb used to prepare mar mutant viruses also lost its activity to enhance homologous mar mutant viruses, strongly suggesting that neutralizing and antibody-dependent enhancing epitopes are present in the same region in the strain FIPV 79-1146. We also discovered that amino acid mutation in the neutralizing epitope reduced viral replication in monocytes/macrophages. We also demonstrated that the mutation or deletion of two nucleotides in 7b gene abrogate the virulence of strain FIPV 79-1146.</description><identifier>ISSN: 0168-1702</identifier><identifier>EISSN: 1872-7492</identifier><identifier>DOI: 10.1016/j.virusres.2010.12.020</identifier><identifier>PMID: 21211540</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Amino Acid Substitution ; amino acids ; Animals ; Antibodies, Neutralizing - genetics ; Antibodies, Neutralizing - immunology ; Antibody-dependent enhancement ; Cats ; Cells, Cultured ; Coronavirus, Feline - genetics ; Coronavirus, Feline - immunology ; Coronavirus, Feline - pathogenicity ; epitopes ; Epitopes - genetics ; Epitopes - immunology ; Feline coronavirus ; Feline infectious peritonitis ; Feline Infectious Peritonitis - immunology ; Feline Infectious Peritonitis - virology ; Feline infectious peritonitis virus ; genes ; Genome, Viral ; Macrophage ; macrophages ; Macrophages - virology ; monoclonal antibodies ; Monoclonal antibody-resistant mutant virus ; Monocyte ; monocytes ; Monocytes - virology ; mutants ; Mutation ; neutralization ; nucleotides ; sequence deletion ; Survival Analysis ; Viral Envelope Proteins - genetics ; Viral Envelope Proteins - immunology ; virulence ; Virulence - genetics ; virus replication ; Virus Replication - physiology ; viruses</subject><ispartof>Virus research, 2011-03, Vol.156 (1-2), p.72-80</ispartof><rights>2011 Elsevier B.V.</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved. 2011 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c592t-aff9321e2d6b90470d252345e01a391091f59833bcee520feaaffbf6ba4325423</citedby><cites>FETCH-LOGICAL-c592t-aff9321e2d6b90470d252345e01a391091f59833bcee520feaaffbf6ba4325423</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.virusres.2010.12.020$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21211540$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Takano, Tomomi</creatorcontrib><creatorcontrib>Tomiyama, Yoshika</creatorcontrib><creatorcontrib>Katoh, Yasuichiroh</creatorcontrib><creatorcontrib>Nakamura, Michiyo</creatorcontrib><creatorcontrib>Satoh, Ryoichi</creatorcontrib><creatorcontrib>Hohdatsu, Tsutomu</creatorcontrib><title>Mutation of neutralizing/antibody-dependent enhancing epitope on spike protein and 7b gene of feline infectious peritonitis virus: Influences of viral replication in monocytes/macrophages and virulence in cats</title><title>Virus research</title><addtitle>Virus Res</addtitle><description>We previously prepared neutralizing monoclonal antibody (MAb)-resistant (mar) mutant viruses using a laboratory strain feline infectious peritonitis virus (FIPV) 79-1146 (Kida et al., 1999). Mar mutant viruses are mutated several amino acids of the neutralizing epitope of Spike protein, compared with the parent strain, FIPV 79-1146. We clarified that MAb used to prepare mar mutant viruses also lost its activity to enhance homologous mar mutant viruses, strongly suggesting that neutralizing and antibody-dependent enhancing epitopes are present in the same region in the strain FIPV 79-1146. We also discovered that amino acid mutation in the neutralizing epitope reduced viral replication in monocytes/macrophages. We also demonstrated that the mutation or deletion of two nucleotides in 7b gene abrogate the virulence of strain FIPV 79-1146.</description><subject>Amino Acid Substitution</subject><subject>amino acids</subject><subject>Animals</subject><subject>Antibodies, Neutralizing - genetics</subject><subject>Antibodies, Neutralizing - immunology</subject><subject>Antibody-dependent enhancement</subject><subject>Cats</subject><subject>Cells, Cultured</subject><subject>Coronavirus, Feline - genetics</subject><subject>Coronavirus, Feline - immunology</subject><subject>Coronavirus, Feline - pathogenicity</subject><subject>epitopes</subject><subject>Epitopes - genetics</subject><subject>Epitopes - immunology</subject><subject>Feline coronavirus</subject><subject>Feline infectious peritonitis</subject><subject>Feline Infectious Peritonitis - immunology</subject><subject>Feline Infectious Peritonitis - virology</subject><subject>Feline infectious peritonitis virus</subject><subject>genes</subject><subject>Genome, Viral</subject><subject>Macrophage</subject><subject>macrophages</subject><subject>Macrophages - virology</subject><subject>monoclonal antibodies</subject><subject>Monoclonal antibody-resistant mutant virus</subject><subject>Monocyte</subject><subject>monocytes</subject><subject>Monocytes - virology</subject><subject>mutants</subject><subject>Mutation</subject><subject>neutralization</subject><subject>nucleotides</subject><subject>sequence deletion</subject><subject>Survival Analysis</subject><subject>Viral Envelope Proteins - genetics</subject><subject>Viral Envelope Proteins - immunology</subject><subject>virulence</subject><subject>Virulence - genetics</subject><subject>virus replication</subject><subject>Virus Replication - physiology</subject><subject>viruses</subject><issn>0168-1702</issn><issn>1872-7492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUk1vEzEQXSEQDYW_UHzjtInt_cpyQKCKQqUiDtCz5fWOkwkbe7F3I4V_yT_qmLQVnDjZ8rz35nnmZdmF4EvBRb3aLQ8Y5hggLiVPj3LJJX-SLcS6kXlTtvJptiDgOhcNl2fZixh3nPO6aOrn2ZkUUoiq5Ivs95d50hN6x7xlDuYp6AF_odustJuw8_0x72EE14ObGLitdoaKDEac_AiMeHHEH8DG4CdAx7TrWdOxDThIihYGpBs6C4a6zJGNEIjqcMLI_nzhLbt2dpjBGYiJQo96YAHGAc3JGcnuvfPmOEFc7bUJftzqDaFTs6QxJHKCESG-zJ5ZPUR4dX-eZ7dXH79ffs5vvn66vvxwk5uqlVOurW0LKUD2ddfysuG9rGRRVsCFLlrBW2Grdl0UnQGoJLegidHZutNlIatSFufZu5PuOHd76A0NiIyrMeBeh6PyGtW_FYdbtfEH1QhRlm1BAm_uBYL_OUOc1B6jgWHQDmhSal03TVGTF0LWJyR9PdLO7WMXwVWKg9qphzioFAclpKI4EPHib4-PtIf9E-D1CWC1V3oTMKrbb6RQU1bkmpfJ5fsTAmiWB4SgosE07x4D7VT1Hv_n4g5kMNut</recordid><startdate>20110301</startdate><enddate>20110301</enddate><creator>Takano, Tomomi</creator><creator>Tomiyama, Yoshika</creator><creator>Katoh, Yasuichiroh</creator><creator>Nakamura, Michiyo</creator><creator>Satoh, Ryoichi</creator><creator>Hohdatsu, Tsutomu</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20110301</creationdate><title>Mutation of neutralizing/antibody-dependent enhancing epitope on spike protein and 7b gene of feline infectious peritonitis virus: Influences of viral replication in monocytes/macrophages and virulence in cats</title><author>Takano, Tomomi ; Tomiyama, Yoshika ; Katoh, Yasuichiroh ; Nakamura, Michiyo ; Satoh, Ryoichi ; Hohdatsu, Tsutomu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c592t-aff9321e2d6b90470d252345e01a391091f59833bcee520feaaffbf6ba4325423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Amino Acid Substitution</topic><topic>amino acids</topic><topic>Animals</topic><topic>Antibodies, Neutralizing - genetics</topic><topic>Antibodies, Neutralizing - immunology</topic><topic>Antibody-dependent enhancement</topic><topic>Cats</topic><topic>Cells, Cultured</topic><topic>Coronavirus, Feline - genetics</topic><topic>Coronavirus, Feline - immunology</topic><topic>Coronavirus, Feline - pathogenicity</topic><topic>epitopes</topic><topic>Epitopes - genetics</topic><topic>Epitopes - immunology</topic><topic>Feline coronavirus</topic><topic>Feline infectious peritonitis</topic><topic>Feline Infectious Peritonitis - immunology</topic><topic>Feline Infectious Peritonitis - virology</topic><topic>Feline infectious peritonitis virus</topic><topic>genes</topic><topic>Genome, Viral</topic><topic>Macrophage</topic><topic>macrophages</topic><topic>Macrophages - virology</topic><topic>monoclonal antibodies</topic><topic>Monoclonal antibody-resistant mutant virus</topic><topic>Monocyte</topic><topic>monocytes</topic><topic>Monocytes - virology</topic><topic>mutants</topic><topic>Mutation</topic><topic>neutralization</topic><topic>nucleotides</topic><topic>sequence deletion</topic><topic>Survival Analysis</topic><topic>Viral Envelope Proteins - genetics</topic><topic>Viral Envelope Proteins - immunology</topic><topic>virulence</topic><topic>Virulence - genetics</topic><topic>virus replication</topic><topic>Virus Replication - physiology</topic><topic>viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takano, Tomomi</creatorcontrib><creatorcontrib>Tomiyama, Yoshika</creatorcontrib><creatorcontrib>Katoh, Yasuichiroh</creatorcontrib><creatorcontrib>Nakamura, Michiyo</creatorcontrib><creatorcontrib>Satoh, Ryoichi</creatorcontrib><creatorcontrib>Hohdatsu, Tsutomu</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Virus research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takano, Tomomi</au><au>Tomiyama, Yoshika</au><au>Katoh, Yasuichiroh</au><au>Nakamura, Michiyo</au><au>Satoh, Ryoichi</au><au>Hohdatsu, Tsutomu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mutation of neutralizing/antibody-dependent enhancing epitope on spike protein and 7b gene of feline infectious peritonitis virus: Influences of viral replication in monocytes/macrophages and virulence in cats</atitle><jtitle>Virus research</jtitle><addtitle>Virus Res</addtitle><date>2011-03-01</date><risdate>2011</risdate><volume>156</volume><issue>1-2</issue><spage>72</spage><epage>80</epage><pages>72-80</pages><issn>0168-1702</issn><eissn>1872-7492</eissn><abstract>We previously prepared neutralizing monoclonal antibody (MAb)-resistant (mar) mutant viruses using a laboratory strain feline infectious peritonitis virus (FIPV) 79-1146 (Kida et al., 1999). Mar mutant viruses are mutated several amino acids of the neutralizing epitope of Spike protein, compared with the parent strain, FIPV 79-1146. We clarified that MAb used to prepare mar mutant viruses also lost its activity to enhance homologous mar mutant viruses, strongly suggesting that neutralizing and antibody-dependent enhancing epitopes are present in the same region in the strain FIPV 79-1146. We also discovered that amino acid mutation in the neutralizing epitope reduced viral replication in monocytes/macrophages. We also demonstrated that the mutation or deletion of two nucleotides in 7b gene abrogate the virulence of strain FIPV 79-1146.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>21211540</pmid><doi>10.1016/j.virusres.2010.12.020</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Substitution amino acids Animals Antibodies, Neutralizing - genetics Antibodies, Neutralizing - immunology Antibody-dependent enhancement Cats Cells, Cultured Coronavirus, Feline - genetics Coronavirus, Feline - immunology Coronavirus, Feline - pathogenicity epitopes Epitopes - genetics Epitopes - immunology Feline coronavirus Feline infectious peritonitis Feline Infectious Peritonitis - immunology Feline Infectious Peritonitis - virology Feline infectious peritonitis virus genes Genome, Viral Macrophage macrophages Macrophages - virology monoclonal antibodies Monoclonal antibody-resistant mutant virus Monocyte monocytes Monocytes - virology mutants Mutation neutralization nucleotides sequence deletion Survival Analysis Viral Envelope Proteins - genetics Viral Envelope Proteins - immunology virulence Virulence - genetics virus replication Virus Replication - physiology viruses |
| title | Mutation of neutralizing/antibody-dependent enhancing epitope on spike protein and 7b gene of feline infectious peritonitis virus: Influences of viral replication in monocytes/macrophages and virulence in cats |
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