Development of fluorescent reverse transcription loop-mediated isothermal amplification (RT-LAMP) using quenching probes for the detection of the Middle East respiratory syndrome coronavirus

•Fluorescent RT-LAMP assays using quenching probes for MERS-CoV were developed.•Quenching probe (QProbe) can solve the problem in turbidity monitoring mechanism.•Only primer-derived signal can be monitored specifically by QProbes.•Two primer sets were developed to enable to confirm MERS case by RT-L...

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Veröffentlicht in:	Journal of virological methods 2018-08, Vol.258, p.41-48
Hauptverfasser:	Shirato, Kazuya, Semba, Shohei, El-Kafrawy, Sherif A., Hassan, Ahmed M., Tolah, Ahmed M., Takayama, Ikuyo, Kageyama, Tsutomu, Notomi, Tsugunori, Kamitani, Wataru, Matsuyama, Shutoku, Azhar, Esam Ibraheem
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals camels Camelus Coronavirus Infections - diagnosis Coronavirus Infections - veterinary cross reaction diagnostic techniques DNA DNA Primers - genetics Fluorescence Humans magnesium MERS coronavirus Middle East respiratory syndrome Middle East respiratory syndrome coronavirus Middle East Respiratory Syndrome Coronavirus - genetics Middle East Respiratory Syndrome Coronavirus - isolation & purification Molecular Diagnostic Techniques - methods monitoring Nucleic Acid Amplification Techniques - methods nucleocapsid oligodeoxyribonucleotides Oligonucleotide Probes - genetics patients quantitative polymerase chain reaction Quenching probe rapid methods reverse transcriptase polymerase chain reaction reverse transcription loop-mediated isothermal amplification RNA RT-LAMP Saudi Arabia Sensitivity and Specificity transcription (genetics) turbidity viruses
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