Response to change in the number of visual stimuli in zebrafish:A behavioural and molecular study
Evidence has shown that a variety of vertebrates, including fish, can discriminate collections of visual items on the basis of their numerousness using an evolutionarily conserved system for approximating numerical magnitude (the so-called Approximate Number System, ANS). Here we combine a habituati...
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| creator | Messina, Andrea Potrich, Davide Schiona, Ilaria Sovrano, Valeria Anna Fraser, Scott E. Brennan, Caroline H. Vallortigara, Giorgio |
| description | Evidence has shown that a variety of vertebrates, including fish, can discriminate collections of visual items on the basis of their numerousness using an evolutionarily conserved system for approximating numerical magnitude (the so-called Approximate Number System, ANS). Here we combine a habituation/dishabituation behavioural task with molecular biology assays to start investigating the neural bases of the ANS in zebrafish. Separate groups of zebrafish underwent a habituation phase with a set of 3 or 9 small red dots, associated with a food reward. The dots changed in size, position and density from trial to trial but maintained their numerousness, and the overall areas of the stimuli was kept constant. During the subsequent dishabituation test, zebrafish faced a change
(i)
in number (from 3 to 9 or
vice versa
with the same overall surface), or
(ii)
in shape (with the same overall surface and number), or
(iii)
in size (with the same shape and number). A control group of zebrafish was shown the same stimuli as during the habituation. RT-qPCR revealed that the telencephalon and thalamus were characterized by the most consistent modulation of the expression of the immediate early genes
c-fos
and
egr-1
upon change in numerousness; in contrast, the retina and optic tectum responded mainly to changes in stimulus size. |
| doi_str_mv | 10.1038/s41598-020-62608-5 |
| format | Article |
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(i)
in number (from 3 to 9 or
vice versa
with the same overall surface), or
(ii)
in shape (with the same overall surface and number), or
(iii)
in size (with the same shape and number). A control group of zebrafish was shown the same stimuli as during the habituation. RT-qPCR revealed that the telencephalon and thalamus were characterized by the most consistent modulation of the expression of the immediate early genes
c-fos
and
egr-1
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(i)
in number (from 3 to 9 or
vice versa
with the same overall surface), or
(ii)
in shape (with the same overall surface and number), or
(iii)
in size (with the same shape and number). A control group of zebrafish was shown the same stimuli as during the habituation. RT-qPCR revealed that the telencephalon and thalamus were characterized by the most consistent modulation of the expression of the immediate early genes
c-fos
and
egr-1
upon change in numerousness; in contrast, the retina and optic tectum responded mainly to changes in stimulus size.</description><subject>38</subject><subject>38/77</subject><subject>631/337</subject><subject>631/378/2649</subject><subject>64</subject><subject>64/116</subject><subject>Animals</subject><subject>c-Fos protein</subject><subject>Danio rerio</subject><subject>Discrimination Learning</subject><subject>EGR-1 protein</subject><subject>Genes, Immediate-Early</subject><subject>Habituation</subject><subject>Habituation, Psychophysiologic</subject><subject>Humanities and Social Sciences</subject><subject>Immediate-early proteins</subject><subject>Male</subject><subject>Molecular biology</subject><subject>multidisciplinary</subject><subject>Number systems</subject><subject>Photic Stimulation</subject><subject>Reinforcement</subject><subject>Retina</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Superior Colliculi - physiology</subject><subject>Superior colliculus</subject><subject>Telencephalon</subject><subject>Telencephalon - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Messina, Andrea</au><au>Potrich, Davide</au><au>Schiona, Ilaria</au><au>Sovrano, Valeria Anna</au><au>Fraser, Scott E.</au><au>Brennan, Caroline H.</au><au>Vallortigara, Giorgio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Response to change in the number of visual stimuli in zebrafish:A behavioural and molecular study</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2020-04-01</date><risdate>2020</risdate><volume>10</volume><issue>1</issue><spage>5769</spage><epage>5769</epage><pages>5769-5769</pages><artnum>5769</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Evidence has shown that a variety of vertebrates, including fish, can discriminate collections of visual items on the basis of their numerousness using an evolutionarily conserved system for approximating numerical magnitude (the so-called Approximate Number System, ANS). Here we combine a habituation/dishabituation behavioural task with molecular biology assays to start investigating the neural bases of the ANS in zebrafish. Separate groups of zebrafish underwent a habituation phase with a set of 3 or 9 small red dots, associated with a food reward. The dots changed in size, position and density from trial to trial but maintained their numerousness, and the overall areas of the stimuli was kept constant. During the subsequent dishabituation test, zebrafish faced a change
(i)
in number (from 3 to 9 or
vice versa
with the same overall surface), or
(ii)
in shape (with the same overall surface and number), or
(iii)
in size (with the same shape and number). A control group of zebrafish was shown the same stimuli as during the habituation. RT-qPCR revealed that the telencephalon and thalamus were characterized by the most consistent modulation of the expression of the immediate early genes
c-fos
and
egr-1
upon change in numerousness; in contrast, the retina and optic tectum responded mainly to changes in stimulus size.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>32238844</pmid><doi>10.1038/s41598-020-62608-5</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-4169-4083</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Nature Free; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry; Springer Nature OA Free Journals |
| subjects | 38 38/77 631/337 631/378/2649 64 64/116 Animals c-Fos protein Danio rerio Discrimination Learning EGR-1 protein Genes, Immediate-Early Habituation Habituation, Psychophysiologic Humanities and Social Sciences Immediate-early proteins Male Molecular biology multidisciplinary Number systems Photic Stimulation Reinforcement Retina Science Science (multidisciplinary) Superior Colliculi - physiology Superior colliculus Telencephalon Telencephalon - physiology Thalamus Thalamus - physiology Visual Perception Visual stimuli Zebrafish Zebrafish - genetics Zebrafish - physiology |
| title | Response to change in the number of visual stimuli in zebrafish:A behavioural and molecular study |
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