Monoclonal antibodies to SARS-associated coronavirus (SARS-CoV): Identification of neutralizing and antibodies reactive to S, N, M and E viral proteins
Monoclonal antibodies (Mabs) against the Urbani strain of the SARS-associated coronavirus (SARS-CoV) were developed and characterized for reactivity to SARS-CoV and SARS-CoV S, N, M, and E proteins using enzyme-linked immunoabsorbent (ELISA), radioimmunoprecipitation, immunofluorescence, Western Blo...
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| Veröffentlicht in: | Journal of virological methods 2005-09, Vol.128 (1), p.21-28 |
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| creator | Tripp, Ralph A. Haynes, Lia M. Moore, Deborah Anderson, Barbara Tamin, Azaibi Harcourt, Brian H. Jones, Les P. Yilla, Mamadi Babcock, Gregory J. Greenough, Thomas Ambrosino, Donna M. Alvarez, Rene Callaway, Justin Cavitt, Sheana Kamrud, Kurt Alterson, Harold Smith, Jonathan Harcourt, Jennifer L. Miao, Congrong Razdan, Raj Comer, James A. Rollin, Pierre E. Ksiazek, Thomas G. Sanchez, Anthony Rota, Paul A. Bellini, William J. Anderson, Larry J. |
| description | Monoclonal antibodies (Mabs) against the Urbani strain of the SARS-associated coronavirus (SARS-CoV) were developed and characterized for reactivity to SARS-CoV and SARS-CoV S, N, M, and E proteins using enzyme-linked immunoabsorbent (ELISA), radioimmunoprecipitation, immunofluorescence, Western Blot and microneutralization assays. Twenty-six mAbs were reactive to SARS-CoV by ELISA, and nine were chosen for detailed characterization. Five mAbs reacted against the S protein, two against the M protein, and one each against the N and E proteins. Two of five S protein mAbs neutralized SARS-CoV infection of Vero E6 cells and reacted to an epitope within amino acids 490–510 in the S protein. While two of the three non-neutralizing antibodies recognized at second epitope within amino acids 270–350. The mAbs characterized should prove useful for developing SARS-CoV diagnostic assays and for studying the biology of infection and pathogenesis of disease. |
| doi_str_mv | 10.1016/j.jviromet.2005.03.021 |
| format | Article |
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Twenty-six mAbs were reactive to SARS-CoV by ELISA, and nine were chosen for detailed characterization. Five mAbs reacted against the S protein, two against the M protein, and one each against the N and E proteins. Two of five S protein mAbs neutralized SARS-CoV infection of Vero E6 cells and reacted to an epitope within amino acids 490–510 in the S protein. While two of the three non-neutralizing antibodies recognized at second epitope within amino acids 270–350. The mAbs characterized should prove useful for developing SARS-CoV diagnostic assays and for studying the biology of infection and pathogenesis of disease.</description><identifier>ISSN: 0166-0934</identifier><identifier>EISSN: 1879-0984</identifier><identifier>DOI: 10.1016/j.jviromet.2005.03.021</identifier><identifier>PMID: 15885812</identifier><identifier>CODEN: JVMEDH</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Animals ; Antibodies, Monoclonal - biosynthesis ; Antibodies, Monoclonal - immunology ; Antibodies, Viral - biosynthesis ; Antibodies, Viral - immunology ; Antibody Specificity ; Biological and medical sciences ; Cell Line ; Chlorocebus aethiops ; Epitope ; Epitope Mapping ; Fundamental and applied biological sciences. Psychology ; Humans ; Immunoassay ; Membrane Glycoproteins - immunology ; Microbiology ; Monoclonal antibody ; Neutralization Tests ; Neutralizing ; Nucleocapsid Proteins - immunology ; SARS Virus - immunology ; SARS-coronavirus ; Severe Acute Respiratory Syndrome - virology ; Spike Glycoprotein, Coronavirus ; Techniques used in virology ; Vero Cells ; Viral Envelope Proteins - immunology ; Viral Matrix Proteins - immunology ; Viral Structural Proteins - immunology ; Virology</subject><ispartof>Journal of virological methods, 2005-09, Vol.128 (1), p.21-28</ispartof><rights>2005 Elsevier B.V.</rights><rights>2005 INIST-CNRS</rights><rights>Copyright © 2005 Elsevier B.V. All rights reserved. 2005 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c530t-1865ca800e27661cc843f4e29dc67baa110fec071cdb738262a23e07873958563</citedby><cites>FETCH-LOGICAL-c530t-1865ca800e27661cc843f4e29dc67baa110fec071cdb738262a23e07873958563</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0166093405001084$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16980830$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15885812$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tripp, Ralph A.</creatorcontrib><creatorcontrib>Haynes, Lia M.</creatorcontrib><creatorcontrib>Moore, Deborah</creatorcontrib><creatorcontrib>Anderson, Barbara</creatorcontrib><creatorcontrib>Tamin, Azaibi</creatorcontrib><creatorcontrib>Harcourt, Brian H.</creatorcontrib><creatorcontrib>Jones, Les P.</creatorcontrib><creatorcontrib>Yilla, Mamadi</creatorcontrib><creatorcontrib>Babcock, Gregory J.</creatorcontrib><creatorcontrib>Greenough, Thomas</creatorcontrib><creatorcontrib>Ambrosino, Donna M.</creatorcontrib><creatorcontrib>Alvarez, Rene</creatorcontrib><creatorcontrib>Callaway, Justin</creatorcontrib><creatorcontrib>Cavitt, Sheana</creatorcontrib><creatorcontrib>Kamrud, Kurt</creatorcontrib><creatorcontrib>Alterson, Harold</creatorcontrib><creatorcontrib>Smith, Jonathan</creatorcontrib><creatorcontrib>Harcourt, Jennifer L.</creatorcontrib><creatorcontrib>Miao, Congrong</creatorcontrib><creatorcontrib>Razdan, Raj</creatorcontrib><creatorcontrib>Comer, James A.</creatorcontrib><creatorcontrib>Rollin, Pierre E.</creatorcontrib><creatorcontrib>Ksiazek, Thomas G.</creatorcontrib><creatorcontrib>Sanchez, Anthony</creatorcontrib><creatorcontrib>Rota, Paul A.</creatorcontrib><creatorcontrib>Bellini, William J.</creatorcontrib><creatorcontrib>Anderson, Larry J.</creatorcontrib><title>Monoclonal antibodies to SARS-associated coronavirus (SARS-CoV): Identification of neutralizing and antibodies reactive to S, N, M and E viral proteins</title><title>Journal of virological methods</title><addtitle>J Virol Methods</addtitle><description>Monoclonal antibodies (Mabs) against the Urbani strain of the SARS-associated coronavirus (SARS-CoV) were developed and characterized for reactivity to SARS-CoV and SARS-CoV S, N, M, and E proteins using enzyme-linked immunoabsorbent (ELISA), radioimmunoprecipitation, immunofluorescence, Western Blot and microneutralization assays. Twenty-six mAbs were reactive to SARS-CoV by ELISA, and nine were chosen for detailed characterization. Five mAbs reacted against the S protein, two against the M protein, and one each against the N and E proteins. Two of five S protein mAbs neutralized SARS-CoV infection of Vero E6 cells and reacted to an epitope within amino acids 490–510 in the S protein. While two of the three non-neutralizing antibodies recognized at second epitope within amino acids 270–350. The mAbs characterized should prove useful for developing SARS-CoV diagnostic assays and for studying the biology of infection and pathogenesis of disease.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - biosynthesis</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibodies, Viral - biosynthesis</subject><subject>Antibodies, Viral - immunology</subject><subject>Antibody Specificity</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Chlorocebus aethiops</subject><subject>Epitope</subject><subject>Epitope Mapping</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Immunoassay</subject><subject>Membrane Glycoproteins - immunology</subject><subject>Microbiology</subject><subject>Monoclonal antibody</subject><subject>Neutralization Tests</subject><subject>Neutralizing</subject><subject>Nucleocapsid Proteins - immunology</subject><subject>SARS Virus - immunology</subject><subject>SARS-coronavirus</subject><subject>Severe Acute Respiratory Syndrome - virology</subject><subject>Spike Glycoprotein, Coronavirus</subject><subject>Techniques used in virology</subject><subject>Vero Cells</subject><subject>Viral Envelope Proteins - immunology</subject><subject>Viral Matrix Proteins - immunology</subject><subject>Viral Structural Proteins - immunology</subject><subject>Virology</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhiMEokvhFSpfQCA1YRwnttMDoloVqNSCRIGr5XUmxausvdjOSvAivC7u7kLLqSdbmm_--Wf-ojiiUFGg_PWyWm5s8CtMVQ3QVsAqqOmDYkal6EroZPOwmGWQ5z9rDoonMS4hg4Kxx8UBbaVsJa1nxe9L77wZvdMj0S7Zhe8tRpI8uTr9fFXqGL2xOmFPjA-ZykOnSF5ui3P_7dUJOe8x9w3W6GS9I34gDqcU9Gh_WXedRfu7wgG1SXaD2wnH5OMxudwiZyQrZw_r4BNaF58WjwY9Rny2fw-Lr-_Ovsw_lBef3p_PTy9K0zJIJZW8NVoCYC04p8bIhg0N1l1vuFhoTSkMaEBQ0y8EkzWvdc0QhBSsa2XL2WHxZqe7nhYr7E3eJdtQ62BXOvxUXlv1f8XZ7-rab5SgtJZQZ4EXe4Hgf0wYk1rZaHActUM_RcUlNG0D4l6QCt5S2sgM8h1ogo8x4PDPDQV1E75aqr_hq5vwFTCVw8-NR3d3uW3bp52B53tAR6PHIWhnbLzleCdBMsjc2x2H-fIbi0FFY9EZ7G1Ak1Tv7X1e_gBOfNKg</recordid><startdate>20050901</startdate><enddate>20050901</enddate><creator>Tripp, Ralph A.</creator><creator>Haynes, Lia M.</creator><creator>Moore, Deborah</creator><creator>Anderson, Barbara</creator><creator>Tamin, Azaibi</creator><creator>Harcourt, Brian H.</creator><creator>Jones, Les P.</creator><creator>Yilla, Mamadi</creator><creator>Babcock, Gregory J.</creator><creator>Greenough, Thomas</creator><creator>Ambrosino, Donna M.</creator><creator>Alvarez, Rene</creator><creator>Callaway, Justin</creator><creator>Cavitt, Sheana</creator><creator>Kamrud, Kurt</creator><creator>Alterson, Harold</creator><creator>Smith, Jonathan</creator><creator>Harcourt, Jennifer L.</creator><creator>Miao, Congrong</creator><creator>Razdan, Raj</creator><creator>Comer, James A.</creator><creator>Rollin, Pierre E.</creator><creator>Ksiazek, Thomas G.</creator><creator>Sanchez, Anthony</creator><creator>Rota, Paul A.</creator><creator>Bellini, William J.</creator><creator>Anderson, Larry J.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20050901</creationdate><title>Monoclonal antibodies to SARS-associated coronavirus (SARS-CoV): Identification of neutralizing and antibodies reactive to S, N, M and E viral proteins</title><author>Tripp, Ralph A. ; Haynes, Lia M. ; Moore, Deborah ; Anderson, Barbara ; Tamin, Azaibi ; Harcourt, Brian H. ; Jones, Les P. ; Yilla, Mamadi ; Babcock, Gregory J. ; Greenough, Thomas ; Ambrosino, Donna M. ; Alvarez, Rene ; Callaway, Justin ; Cavitt, Sheana ; Kamrud, Kurt ; Alterson, Harold ; Smith, Jonathan ; Harcourt, Jennifer L. ; Miao, Congrong ; Razdan, Raj ; Comer, James A. ; Rollin, Pierre E. ; Ksiazek, Thomas G. ; Sanchez, Anthony ; Rota, Paul A. ; Bellini, William J. ; Anderson, Larry J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c530t-1865ca800e27661cc843f4e29dc67baa110fec071cdb738262a23e07873958563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - biosynthesis</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibodies, Viral - biosynthesis</topic><topic>Antibodies, Viral - immunology</topic><topic>Antibody Specificity</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Chlorocebus aethiops</topic><topic>Epitope</topic><topic>Epitope Mapping</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Immunoassay</topic><topic>Membrane Glycoproteins - immunology</topic><topic>Microbiology</topic><topic>Monoclonal antibody</topic><topic>Neutralization Tests</topic><topic>Neutralizing</topic><topic>Nucleocapsid Proteins - immunology</topic><topic>SARS Virus - immunology</topic><topic>SARS-coronavirus</topic><topic>Severe Acute Respiratory Syndrome - virology</topic><topic>Spike Glycoprotein, Coronavirus</topic><topic>Techniques used in virology</topic><topic>Vero Cells</topic><topic>Viral Envelope Proteins - immunology</topic><topic>Viral Matrix Proteins - immunology</topic><topic>Viral Structural Proteins - immunology</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tripp, Ralph A.</creatorcontrib><creatorcontrib>Haynes, Lia M.</creatorcontrib><creatorcontrib>Moore, Deborah</creatorcontrib><creatorcontrib>Anderson, Barbara</creatorcontrib><creatorcontrib>Tamin, Azaibi</creatorcontrib><creatorcontrib>Harcourt, Brian H.</creatorcontrib><creatorcontrib>Jones, Les P.</creatorcontrib><creatorcontrib>Yilla, Mamadi</creatorcontrib><creatorcontrib>Babcock, Gregory J.</creatorcontrib><creatorcontrib>Greenough, Thomas</creatorcontrib><creatorcontrib>Ambrosino, Donna M.</creatorcontrib><creatorcontrib>Alvarez, Rene</creatorcontrib><creatorcontrib>Callaway, Justin</creatorcontrib><creatorcontrib>Cavitt, Sheana</creatorcontrib><creatorcontrib>Kamrud, Kurt</creatorcontrib><creatorcontrib>Alterson, Harold</creatorcontrib><creatorcontrib>Smith, Jonathan</creatorcontrib><creatorcontrib>Harcourt, Jennifer L.</creatorcontrib><creatorcontrib>Miao, Congrong</creatorcontrib><creatorcontrib>Razdan, Raj</creatorcontrib><creatorcontrib>Comer, James A.</creatorcontrib><creatorcontrib>Rollin, Pierre E.</creatorcontrib><creatorcontrib>Ksiazek, Thomas G.</creatorcontrib><creatorcontrib>Sanchez, Anthony</creatorcontrib><creatorcontrib>Rota, Paul A.</creatorcontrib><creatorcontrib>Bellini, William J.</creatorcontrib><creatorcontrib>Anderson, Larry J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of virological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tripp, Ralph A.</au><au>Haynes, Lia M.</au><au>Moore, Deborah</au><au>Anderson, Barbara</au><au>Tamin, Azaibi</au><au>Harcourt, Brian H.</au><au>Jones, Les P.</au><au>Yilla, Mamadi</au><au>Babcock, Gregory J.</au><au>Greenough, Thomas</au><au>Ambrosino, Donna M.</au><au>Alvarez, Rene</au><au>Callaway, Justin</au><au>Cavitt, Sheana</au><au>Kamrud, Kurt</au><au>Alterson, Harold</au><au>Smith, Jonathan</au><au>Harcourt, Jennifer L.</au><au>Miao, Congrong</au><au>Razdan, Raj</au><au>Comer, James A.</au><au>Rollin, Pierre E.</au><au>Ksiazek, Thomas G.</au><au>Sanchez, Anthony</au><au>Rota, Paul A.</au><au>Bellini, William J.</au><au>Anderson, Larry J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monoclonal antibodies to SARS-associated coronavirus (SARS-CoV): Identification of neutralizing and antibodies reactive to S, N, M and E viral proteins</atitle><jtitle>Journal of virological methods</jtitle><addtitle>J Virol Methods</addtitle><date>2005-09-01</date><risdate>2005</risdate><volume>128</volume><issue>1</issue><spage>21</spage><epage>28</epage><pages>21-28</pages><issn>0166-0934</issn><eissn>1879-0984</eissn><coden>JVMEDH</coden><abstract>Monoclonal antibodies (Mabs) against the Urbani strain of the SARS-associated coronavirus (SARS-CoV) were developed and characterized for reactivity to SARS-CoV and SARS-CoV S, N, M, and E proteins using enzyme-linked immunoabsorbent (ELISA), radioimmunoprecipitation, immunofluorescence, Western Blot and microneutralization assays. Twenty-six mAbs were reactive to SARS-CoV by ELISA, and nine were chosen for detailed characterization. Five mAbs reacted against the S protein, two against the M protein, and one each against the N and E proteins. Two of five S protein mAbs neutralized SARS-CoV infection of Vero E6 cells and reacted to an epitope within amino acids 490–510 in the S protein. While two of the three non-neutralizing antibodies recognized at second epitope within amino acids 270–350. The mAbs characterized should prove useful for developing SARS-CoV diagnostic assays and for studying the biology of infection and pathogenesis of disease.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>15885812</pmid><doi>10.1016/j.jviromet.2005.03.021</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of virological methods, 2005-09, Vol.128 (1), p.21-28 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - immunology Antibodies, Viral - biosynthesis Antibodies, Viral - immunology Antibody Specificity Biological and medical sciences Cell Line Chlorocebus aethiops Epitope Epitope Mapping Fundamental and applied biological sciences. Psychology Humans Immunoassay Membrane Glycoproteins - immunology Microbiology Monoclonal antibody Neutralization Tests Neutralizing Nucleocapsid Proteins - immunology SARS Virus - immunology SARS-coronavirus Severe Acute Respiratory Syndrome - virology Spike Glycoprotein, Coronavirus Techniques used in virology Vero Cells Viral Envelope Proteins - immunology Viral Matrix Proteins - immunology Viral Structural Proteins - immunology Virology |
| title | Monoclonal antibodies to SARS-associated coronavirus (SARS-CoV): Identification of neutralizing and antibodies reactive to S, N, M and E viral proteins |
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