A novel biosensor based on serum antibody immobilization for rapid detection of viral antigens
► A label-free serum antibody-based biosensor was developed for rapid detection of viral antigens. ► The simple electrical measurements were performed by non-Faradic impedance. ► The detection range of this biosensor is 1–10μg/ml JEV antigens. ► The stable signals are measured in about 20min. ► The...
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| Veröffentlicht in: | Talanta (Oxford) 2011-10, Vol.86, p.271-277 |
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| creator | Huy, Tran Quang Hanh, Nguyen Thi Hong Thuy, Nguyen Thanh Chung, Pham Van Nga, Phan Thi Tuan, Mai Anh |
| description | ► A label-free serum antibody-based biosensor was developed for rapid detection of viral antigens. ► The simple electrical measurements were performed by non-Faradic impedance. ► The detection range of this biosensor is 1–10μg/ml JEV antigens. ► The stable signals are measured in about 20min. ► The biosensor revealed the potential application in the case of possible outbreaks.
In this paper, we represent a label-free biosensor based on immobilization of serum antibodies for rapid detection of viral antigens. Human serum containing specific antibodies against Japanese encephalitis virus (JEV) was immobilized on a silanized surface of an interdigitated sensor via protein A/glutaraldehyde for electrical detection of JEV antigens. The effective immobilization of serum antibodies on the sensor surface was verified by Fourier transform infrared spectrometry and fluorescence microscopy. The signal of the biosensor obtained by the differential voltage converted from the change into non-Faradic impedance resulting from the specific binding of JEV antigens on the surface of the sensor. The detection analyzed indicates that the detection range of this biosensor is 1–10μg/ml JEV antigens, with a detection limit of 0.75μg/ml and that stable signals are measured in about 20min. This study presents a useful biosensor with a high selectivity for rapid and simple detection of JEV antigens, and it also proposes the biosensor as a future diagnostic tool for rapid and direct detection of viral antigens in clinical samples for preliminary pathogenic screenings in the case of possible outbreaks. |
| doi_str_mv | 10.1016/j.talanta.2011.09.012 |
| format | Article |
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In this paper, we represent a label-free biosensor based on immobilization of serum antibodies for rapid detection of viral antigens. Human serum containing specific antibodies against Japanese encephalitis virus (JEV) was immobilized on a silanized surface of an interdigitated sensor via protein A/glutaraldehyde for electrical detection of JEV antigens. The effective immobilization of serum antibodies on the sensor surface was verified by Fourier transform infrared spectrometry and fluorescence microscopy. The signal of the biosensor obtained by the differential voltage converted from the change into non-Faradic impedance resulting from the specific binding of JEV antigens on the surface of the sensor. The detection analyzed indicates that the detection range of this biosensor is 1–10μg/ml JEV antigens, with a detection limit of 0.75μg/ml and that stable signals are measured in about 20min. This study presents a useful biosensor with a high selectivity for rapid and simple detection of JEV antigens, and it also proposes the biosensor as a future diagnostic tool for rapid and direct detection of viral antigens in clinical samples for preliminary pathogenic screenings in the case of possible outbreaks.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/j.talanta.2011.09.012</identifier><identifier>PMID: 22063541</identifier><identifier>CODEN: TLNTA2</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analytical chemistry ; Antibodies ; Antibodies, Immobilized - analysis ; Antibodies, Immobilized - blood ; Antibodies, Immobilized - immunology ; Antibodies, Viral - analysis ; Antibodies, Viral - blood ; Antibodies, Viral - immunology ; Antigens, Viral - analysis ; Biological and medical sciences ; Biosensing Techniques - methods ; Biosensors ; Biotechnology ; Chemistry ; Encephalitis Virus, Japanese - immunology ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; General, instrumentation ; Humans ; Immunosensor ; Japanese encephalitis virus ; Methods. Procedures. Technologies ; Outbreak ; Preliminary pathogenic screening ; Serum antibody immobilization ; Time Factors ; Various methods and equipments ; Viral antigen detection</subject><ispartof>Talanta (Oxford), 2011-10, Vol.86, p.271-277</ispartof><rights>2011 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved. 2011 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c528t-6a68aff17d9df7161971cef9f4c5e71c1de2d93c76ba3a61af62d19d9b55ea303</citedby><cites>FETCH-LOGICAL-c528t-6a68aff17d9df7161971cef9f4c5e71c1de2d93c76ba3a61af62d19d9b55ea303</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.talanta.2011.09.012$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,315,782,786,887,3554,27933,27934,46004</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25232861$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22063541$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huy, Tran Quang</creatorcontrib><creatorcontrib>Hanh, Nguyen Thi Hong</creatorcontrib><creatorcontrib>Thuy, Nguyen Thanh</creatorcontrib><creatorcontrib>Chung, Pham Van</creatorcontrib><creatorcontrib>Nga, Phan Thi</creatorcontrib><creatorcontrib>Tuan, Mai Anh</creatorcontrib><title>A novel biosensor based on serum antibody immobilization for rapid detection of viral antigens</title><title>Talanta (Oxford)</title><addtitle>Talanta</addtitle><description>► A label-free serum antibody-based biosensor was developed for rapid detection of viral antigens. ► The simple electrical measurements were performed by non-Faradic impedance. ► The detection range of this biosensor is 1–10μg/ml JEV antigens. ► The stable signals are measured in about 20min. ► The biosensor revealed the potential application in the case of possible outbreaks.
In this paper, we represent a label-free biosensor based on immobilization of serum antibodies for rapid detection of viral antigens. Human serum containing specific antibodies against Japanese encephalitis virus (JEV) was immobilized on a silanized surface of an interdigitated sensor via protein A/glutaraldehyde for electrical detection of JEV antigens. The effective immobilization of serum antibodies on the sensor surface was verified by Fourier transform infrared spectrometry and fluorescence microscopy. The signal of the biosensor obtained by the differential voltage converted from the change into non-Faradic impedance resulting from the specific binding of JEV antigens on the surface of the sensor. The detection analyzed indicates that the detection range of this biosensor is 1–10μg/ml JEV antigens, with a detection limit of 0.75μg/ml and that stable signals are measured in about 20min. This study presents a useful biosensor with a high selectivity for rapid and simple detection of JEV antigens, and it also proposes the biosensor as a future diagnostic tool for rapid and direct detection of viral antigens in clinical samples for preliminary pathogenic screenings in the case of possible outbreaks.</description><subject>Analytical chemistry</subject><subject>Antibodies</subject><subject>Antibodies, Immobilized - analysis</subject><subject>Antibodies, Immobilized - blood</subject><subject>Antibodies, Immobilized - immunology</subject><subject>Antibodies, Viral - analysis</subject><subject>Antibodies, Viral - blood</subject><subject>Antibodies, Viral - immunology</subject><subject>Antigens, Viral - analysis</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Chemistry</subject><subject>Encephalitis Virus, Japanese - immunology</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General, instrumentation</subject><subject>Humans</subject><subject>Immunosensor</subject><subject>Japanese encephalitis virus</subject><subject>Methods. Procedures. Technologies</subject><subject>Outbreak</subject><subject>Preliminary pathogenic screening</subject><subject>Serum antibody immobilization</subject><subject>Time Factors</subject><subject>Various methods and equipments</subject><subject>Viral antigen detection</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-PFCEQxYnRuOPqR9BwMZ66pWAamotms_FfsokXvUpoKFYm3c0IPZPsfnqZnXHV054g1K8er-oR8hJYCwzk20272NHOi205A2iZbhnwR2QFvRKN6JR4TFaMCd1oWLMz8qyUDWOMCyaekjPOmRTdGlbkxwWd0x5HOsRUcC4p08EW9DTNtGDeTbT-EYfkb2icpjTEMd7aJdZqqGi22-ipxwXd3VsKdB-zHe-arqvcc_Ik2LHgi9N5Tr5__PDt8nNz9fXTl8uLq8Z1vF8aaWVvQwDltQ8KJGgFDoMOa9dhvYJH7rVwSg5WWAk2SO5Bez10Hdo60zl5d9Td7oYJvcN5qTbMNsfJ5huTbDT_V-b401ynvVEAoDpeBd6cBHL6tcOymCkWh2NdMaZdMbpykq-FephkvAeu1MFUdyRdTqVkDPd-gJlDiGZjTiGaQ4iGaVNDrH2v_h3mvutPahV4fQJscXYM2c4ulr9cnYf38sC9P3JYV7-PmE1xEWeHPuaamPEpPmDlNwZsv-0</recordid><startdate>20111030</startdate><enddate>20111030</enddate><creator>Huy, Tran Quang</creator><creator>Hanh, Nguyen Thi Hong</creator><creator>Thuy, Nguyen Thanh</creator><creator>Chung, Pham Van</creator><creator>Nga, Phan Thi</creator><creator>Tuan, Mai Anh</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U9</scope><scope>H94</scope><scope>5PM</scope></search><sort><creationdate>20111030</creationdate><title>A novel biosensor based on serum antibody immobilization for rapid detection of viral antigens</title><author>Huy, Tran Quang ; Hanh, Nguyen Thi Hong ; Thuy, Nguyen Thanh ; Chung, Pham Van ; Nga, Phan Thi ; Tuan, Mai Anh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c528t-6a68aff17d9df7161971cef9f4c5e71c1de2d93c76ba3a61af62d19d9b55ea303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Analytical chemistry</topic><topic>Antibodies</topic><topic>Antibodies, Immobilized - analysis</topic><topic>Antibodies, Immobilized - blood</topic><topic>Antibodies, Immobilized - immunology</topic><topic>Antibodies, Viral - analysis</topic><topic>Antibodies, Viral - blood</topic><topic>Antibodies, Viral - immunology</topic><topic>Antigens, Viral - analysis</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Chemistry</topic><topic>Encephalitis Virus, Japanese - immunology</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General, instrumentation</topic><topic>Humans</topic><topic>Immunosensor</topic><topic>Japanese encephalitis virus</topic><topic>Methods. Procedures. Technologies</topic><topic>Outbreak</topic><topic>Preliminary pathogenic screening</topic><topic>Serum antibody immobilization</topic><topic>Time Factors</topic><topic>Various methods and equipments</topic><topic>Viral antigen detection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huy, Tran Quang</creatorcontrib><creatorcontrib>Hanh, Nguyen Thi Hong</creatorcontrib><creatorcontrib>Thuy, Nguyen Thanh</creatorcontrib><creatorcontrib>Chung, Pham Van</creatorcontrib><creatorcontrib>Nga, Phan Thi</creatorcontrib><creatorcontrib>Tuan, Mai Anh</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huy, Tran Quang</au><au>Hanh, Nguyen Thi Hong</au><au>Thuy, Nguyen Thanh</au><au>Chung, Pham Van</au><au>Nga, Phan Thi</au><au>Tuan, Mai Anh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel biosensor based on serum antibody immobilization for rapid detection of viral antigens</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>2011-10-30</date><risdate>2011</risdate><volume>86</volume><spage>271</spage><epage>277</epage><pages>271-277</pages><issn>0039-9140</issn><eissn>1873-3573</eissn><coden>TLNTA2</coden><abstract>► A label-free serum antibody-based biosensor was developed for rapid detection of viral antigens. ► The simple electrical measurements were performed by non-Faradic impedance. ► The detection range of this biosensor is 1–10μg/ml JEV antigens. ► The stable signals are measured in about 20min. ► The biosensor revealed the potential application in the case of possible outbreaks.
In this paper, we represent a label-free biosensor based on immobilization of serum antibodies for rapid detection of viral antigens. Human serum containing specific antibodies against Japanese encephalitis virus (JEV) was immobilized on a silanized surface of an interdigitated sensor via protein A/glutaraldehyde for electrical detection of JEV antigens. The effective immobilization of serum antibodies on the sensor surface was verified by Fourier transform infrared spectrometry and fluorescence microscopy. The signal of the biosensor obtained by the differential voltage converted from the change into non-Faradic impedance resulting from the specific binding of JEV antigens on the surface of the sensor. The detection analyzed indicates that the detection range of this biosensor is 1–10μg/ml JEV antigens, with a detection limit of 0.75μg/ml and that stable signals are measured in about 20min. This study presents a useful biosensor with a high selectivity for rapid and simple detection of JEV antigens, and it also proposes the biosensor as a future diagnostic tool for rapid and direct detection of viral antigens in clinical samples for preliminary pathogenic screenings in the case of possible outbreaks.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>22063541</pmid><doi>10.1016/j.talanta.2011.09.012</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Analytical chemistry Antibodies Antibodies, Immobilized - analysis Antibodies, Immobilized - blood Antibodies, Immobilized - immunology Antibodies, Viral - analysis Antibodies, Viral - blood Antibodies, Viral - immunology Antigens, Viral - analysis Biological and medical sciences Biosensing Techniques - methods Biosensors Biotechnology Chemistry Encephalitis Virus, Japanese - immunology Exact sciences and technology Fundamental and applied biological sciences. Psychology General, instrumentation Humans Immunosensor Japanese encephalitis virus Methods. Procedures. Technologies Outbreak Preliminary pathogenic screening Serum antibody immobilization Time Factors Various methods and equipments Viral antigen detection |
| title | A novel biosensor based on serum antibody immobilization for rapid detection of viral antigens |
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