Polymerase Chain Reaction Is More Sensitive than Viral Culture and Antigen Testing for the Detection of Respiratory Viruses in Adults with Hematological Cancer and Pneumonia
We retrospectively analyzed the value of polymerase chain reaction (PCR) for the detection of respiratory viral infections in 43 patients with hematological cancer whose bronchoalveolar lavage (BAL) samples had been stored. In addition, 17 nose-throat (NT) swabs and 29 blood samples had been obtaine...
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| Veröffentlicht in: | Clinical infectious diseases 2002-01, Vol.34 (2), p.177-183 |
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| container_title | Clinical infectious diseases |
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| creator | Elden, Leontine J. R. van Kraaij, Marian G. J. van Nijhuis, Monique Hendriksen, Karin A. W. Dekker, Ad W. Rozenberg-Arska, Maja Loon, Anton M. van |
| description | We retrospectively analyzed the value of polymerase chain reaction (PCR) for the detection of respiratory viral infections in 43 patients with hematological cancer whose bronchoalveolar lavage (BAL) samples had been stored. In addition, 17 nose-throat (NT) swabs and 29 blood samples had been obtained. PCR was performed to detect parainfluenza viruses 1-3, respiratory syncytial virus, rhinovirus, influenza viruses A and B, enteroviruses, and coronaviruses. Viral cultures or antigen testing of BAL samples revealed 9 respiratory viruses in 8 patients. By use of PCR, 8 more respiratory viruses were detected in another 7 patients, increasing the rate of identification from 19% to 35% (P < .0005). Available NT swabs yielded the same results with PCR as did BAL samples. We conclude that PCR is more sensitive than viral culture or antigen or serologic testing for detection of respiratory viruses in patients with hematological malignancies, and that it offers the possibility for early, more rapid diagnosis. |
| doi_str_mv | 10.1086/338238 |
| format | Article |
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R. van ; Kraaij, Marian G. J. van ; Nijhuis, Monique ; Hendriksen, Karin A. W. ; Dekker, Ad W. ; Rozenberg-Arska, Maja ; Loon, Anton M. van</creator><creatorcontrib>Elden, Leontine J. R. van ; Kraaij, Marian G. J. van ; Nijhuis, Monique ; Hendriksen, Karin A. W. ; Dekker, Ad W. ; Rozenberg-Arska, Maja ; Loon, Anton M. van</creatorcontrib><description>We retrospectively analyzed the value of polymerase chain reaction (PCR) for the detection of respiratory viral infections in 43 patients with hematological cancer whose bronchoalveolar lavage (BAL) samples had been stored. In addition, 17 nose-throat (NT) swabs and 29 blood samples had been obtained. PCR was performed to detect parainfluenza viruses 1-3, respiratory syncytial virus, rhinovirus, influenza viruses A and B, enteroviruses, and coronaviruses. Viral cultures or antigen testing of BAL samples revealed 9 respiratory viruses in 8 patients. By use of PCR, 8 more respiratory viruses were detected in another 7 patients, increasing the rate of identification from 19% to 35% (P < .0005). Available NT swabs yielded the same results with PCR as did BAL samples. We conclude that PCR is more sensitive than viral culture or antigen or serologic testing for detection of respiratory viruses in patients with hematological malignancies, and that it offers the possibility for early, more rapid diagnosis.</description><identifier>ISSN: 1058-4838</identifier><identifier>EISSN: 1537-6591</identifier><identifier>DOI: 10.1086/338238</identifier><identifier>PMID: 11740705</identifier><identifier>CODEN: CIDIEL</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>Adenoviruses ; Adolescent ; Adult ; Aged ; Antigens ; Antigens, Viral - analysis ; Biological and medical sciences ; Bone marrow ; Female ; Hematologic Neoplasms - virology ; Human respiratory syncytial virus ; Human viral diseases ; Humans ; Immunocompromised hosts ; Infections ; Infectious diseases ; Major ; Major Articles ; Male ; Medical sciences ; Middle Aged ; Paramyxoviridae infections ; Pneumonia, Viral - virology ; Polymerase chain reaction ; Polymerase Chain Reaction - methods ; Retrospective Studies ; RNA Virus Infections - diagnosis ; RNA Viruses - chemistry ; RNA Viruses - genetics ; RNA Viruses - growth & development ; RNA Viruses - isolation & purification ; RNA, Viral - genetics ; Sensitivity and Specificity ; Viral diseases ; Viral diseases of the respiratory system and ent viral diseases ; Viral pneumonia ; Virus Cultivation ; Viruses</subject><ispartof>Clinical infectious diseases, 2002-01, Vol.34 (2), p.177-183</ispartof><rights>Copyright 2002 The Infectious Diseases Society of America</rights><rights>2002 by the Infectious Diseases Society of America 2002</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c536t-7e543eda23894abd2d8169cb784a124ab1ed59455fb31c955bb7a1b0e25c5cfe3</citedby><cites>FETCH-LOGICAL-c536t-7e543eda23894abd2d8169cb784a124ab1ed59455fb31c955bb7a1b0e25c5cfe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4461834$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4461834$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,780,784,803,885,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13424452$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11740705$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Elden, Leontine J. R. van</creatorcontrib><creatorcontrib>Kraaij, Marian G. J. van</creatorcontrib><creatorcontrib>Nijhuis, Monique</creatorcontrib><creatorcontrib>Hendriksen, Karin A. W.</creatorcontrib><creatorcontrib>Dekker, Ad W.</creatorcontrib><creatorcontrib>Rozenberg-Arska, Maja</creatorcontrib><creatorcontrib>Loon, Anton M. van</creatorcontrib><title>Polymerase Chain Reaction Is More Sensitive than Viral Culture and Antigen Testing for the Detection of Respiratory Viruses in Adults with Hematological Cancer and Pneumonia</title><title>Clinical infectious diseases</title><addtitle>Clinical Infectious Diseases</addtitle><addtitle>Clinical Infectious Diseases</addtitle><description>We retrospectively analyzed the value of polymerase chain reaction (PCR) for the detection of respiratory viral infections in 43 patients with hematological cancer whose bronchoalveolar lavage (BAL) samples had been stored. In addition, 17 nose-throat (NT) swabs and 29 blood samples had been obtained. PCR was performed to detect parainfluenza viruses 1-3, respiratory syncytial virus, rhinovirus, influenza viruses A and B, enteroviruses, and coronaviruses. Viral cultures or antigen testing of BAL samples revealed 9 respiratory viruses in 8 patients. By use of PCR, 8 more respiratory viruses were detected in another 7 patients, increasing the rate of identification from 19% to 35% (P < .0005). Available NT swabs yielded the same results with PCR as did BAL samples. We conclude that PCR is more sensitive than viral culture or antigen or serologic testing for detection of respiratory viruses in patients with hematological malignancies, and that it offers the possibility for early, more rapid diagnosis.</description><subject>Adenoviruses</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Antigens</subject><subject>Antigens, Viral - analysis</subject><subject>Biological and medical sciences</subject><subject>Bone marrow</subject><subject>Female</subject><subject>Hematologic Neoplasms - virology</subject><subject>Human respiratory syncytial virus</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Immunocompromised hosts</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Major</subject><subject>Major Articles</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Paramyxoviridae infections</subject><subject>Pneumonia, Viral - virology</subject><subject>Polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Retrospective Studies</subject><subject>RNA Virus Infections - diagnosis</subject><subject>RNA Viruses - chemistry</subject><subject>RNA Viruses - genetics</subject><subject>RNA Viruses - growth & development</subject><subject>RNA Viruses - isolation & purification</subject><subject>RNA, Viral - genetics</subject><subject>Sensitivity and Specificity</subject><subject>Viral diseases</subject><subject>Viral diseases of the respiratory system and ent viral diseases</subject><subject>Viral pneumonia</subject><subject>Virus Cultivation</subject><subject>Viruses</subject><issn>1058-4838</issn><issn>1537-6591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkstu1DAUhiMEoqXAEyBkFrAL2LGdOJtKo-EyRQVKKRexsRznZMYlsQfbKcxD8Y54yKgtC8TKlv_P_7lm2X2CnxIsymeUioKKG9k-4bTKS16Tm-mOuciZoGIvuxPCOcaECMxvZ3uEVAxXmO9nv05cvxnAqwBovlLGolNQOhpn0VFAb5wH9AFsMNFcAIorZdEn41WP5mMfxyQq26KZjWYJFp1BiMYuUed8QgE9hwiTleuSbVinn9H5zdZiDBBQijZrk1FAP0xcoQUMSe_d0uhtBGU1-D8BTiyMg7NG3c1udaoPcG93HmQfX744my_y43evjuaz41xzWsa8As4otCp1pGaqaYtWkLLWTSWYIkV6IdDymnHeNZTomvOmqRRpMBRcc90BPcgOJ9_12AzQarAxFS3X3gzKb6RTRv6tWLOSS3chK4LrmhTJ4MnOwLvvY-qLHEzQ0PfKghtD4igjZVn_FyQipUnxNVB7F4KH7jIbguV2BeS0Agl8eD33K2w38wQ83gEqpEZ3PjXahCuOsoIxvq3h0cS5cf3vYA8m5jykyV5SjJVEUJbkfJJNiPDzUlb-mywrWnG5-PJVvidv2evy9LNc0N-q9d7y</recordid><startdate>20020115</startdate><enddate>20020115</enddate><creator>Elden, Leontine J. R. van</creator><creator>Kraaij, Marian G. J. van</creator><creator>Nijhuis, Monique</creator><creator>Hendriksen, Karin A. W.</creator><creator>Dekker, Ad W.</creator><creator>Rozenberg-Arska, Maja</creator><creator>Loon, Anton M. van</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20020115</creationdate><title>Polymerase Chain Reaction Is More Sensitive than Viral Culture and Antigen Testing for the Detection of Respiratory Viruses in Adults with Hematological Cancer and Pneumonia</title><author>Elden, Leontine J. R. van ; Kraaij, Marian G. J. van ; Nijhuis, Monique ; Hendriksen, Karin A. 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R. van</creatorcontrib><creatorcontrib>Kraaij, Marian G. J. van</creatorcontrib><creatorcontrib>Nijhuis, Monique</creatorcontrib><creatorcontrib>Hendriksen, Karin A. W.</creatorcontrib><creatorcontrib>Dekker, Ad W.</creatorcontrib><creatorcontrib>Rozenberg-Arska, Maja</creatorcontrib><creatorcontrib>Loon, Anton M. van</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Clinical infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Elden, Leontine J. R. van</au><au>Kraaij, Marian G. J. van</au><au>Nijhuis, Monique</au><au>Hendriksen, Karin A. W.</au><au>Dekker, Ad W.</au><au>Rozenberg-Arska, Maja</au><au>Loon, Anton M. van</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polymerase Chain Reaction Is More Sensitive than Viral Culture and Antigen Testing for the Detection of Respiratory Viruses in Adults with Hematological Cancer and Pneumonia</atitle><jtitle>Clinical infectious diseases</jtitle><stitle>Clinical Infectious Diseases</stitle><addtitle>Clinical Infectious Diseases</addtitle><date>2002-01-15</date><risdate>2002</risdate><volume>34</volume><issue>2</issue><spage>177</spage><epage>183</epage><pages>177-183</pages><issn>1058-4838</issn><eissn>1537-6591</eissn><coden>CIDIEL</coden><abstract>We retrospectively analyzed the value of polymerase chain reaction (PCR) for the detection of respiratory viral infections in 43 patients with hematological cancer whose bronchoalveolar lavage (BAL) samples had been stored. In addition, 17 nose-throat (NT) swabs and 29 blood samples had been obtained. PCR was performed to detect parainfluenza viruses 1-3, respiratory syncytial virus, rhinovirus, influenza viruses A and B, enteroviruses, and coronaviruses. Viral cultures or antigen testing of BAL samples revealed 9 respiratory viruses in 8 patients. By use of PCR, 8 more respiratory viruses were detected in another 7 patients, increasing the rate of identification from 19% to 35% (P < .0005). Available NT swabs yielded the same results with PCR as did BAL samples. We conclude that PCR is more sensitive than viral culture or antigen or serologic testing for detection of respiratory viruses in patients with hematological malignancies, and that it offers the possibility for early, more rapid diagnosis.</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>11740705</pmid><doi>10.1086/338238</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Adenoviruses Adolescent Adult Aged Antigens Antigens, Viral - analysis Biological and medical sciences Bone marrow Female Hematologic Neoplasms - virology Human respiratory syncytial virus Human viral diseases Humans Immunocompromised hosts Infections Infectious diseases Major Major Articles Male Medical sciences Middle Aged Paramyxoviridae infections Pneumonia, Viral - virology Polymerase chain reaction Polymerase Chain Reaction - methods Retrospective Studies RNA Virus Infections - diagnosis RNA Viruses - chemistry RNA Viruses - genetics RNA Viruses - growth & development RNA Viruses - isolation & purification RNA, Viral - genetics Sensitivity and Specificity Viral diseases Viral diseases of the respiratory system and ent viral diseases Viral pneumonia Virus Cultivation Viruses |
| title | Polymerase Chain Reaction Is More Sensitive than Viral Culture and Antigen Testing for the Detection of Respiratory Viruses in Adults with Hematological Cancer and Pneumonia |
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