A Simplified and Standardized Polymerase Chain Reaction Format for the Diagnosis of Leishmaniasis

Background. Definite diagnosis of Leishmania infections is based on demonstration of the parasite by microscopic analysis of tissue biopsy specimens or aspirate samples. However, microscopy generally shows low sensitivity and requires invasive sampling. Methods. We describe here the development of a...

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Veröffentlicht in:	The Journal of infectious diseases 2008-11, Vol.198 (10), p.1565-1572
Hauptverfasser:	Deborggraeve, Stijn, Laurent, Thierry, Espinosa, Diego, Van der Auwera, Gert, Mbuchi, Margaret, Wasunna, Monique, El-Safi, Sayda, Al-Basheer, Ahmed Almustafa, Arévalo, Jorge, Miranda-Verástegui, César, Leclipteux, Thierry, Mertens, Pascal, Dujardin, Jean-Claude, Herdewijn, Piet, Büscher, Philippe
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Biological and medical sciences Biopsies Blood DNA Fundamental and applied biological sciences. Psychology Human protozoal diseases Humans Infections Infectious diseases Leishmania Leishmania - genetics Leishmania - isolation & purification Leishmaniasis Leishmaniasis - blood Leishmaniasis - diagnosis Leshmaniasis Lesions Major and Brief Reports Medical sciences Microbiology Molecular Sequence Data Parasites Parasitic diseases Polymerase chain reaction Polymerase Chain Reaction - methods Polymerase Chain Reaction - standards Protozoal diseases RNA, Ribosomal, 18S - genetics Sensitivity and Specificity Sequence Alignment Specimens Visceral leishmaniasis
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