Genome-wide identification and expression analysis of the CBF/DREB1 gene family in lettuce
The C-repeat binding factor (CBF)/dehydration-responsive element binding (DREB1) proteins play a prominent role in freezing tolerance and are highly conserved in higher plants. Here we performed a genome-wide search of the CBF/DREB1 gene family in lettuce ( Lactuca sativa L.) and identified 14 membe...
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| description | The C-repeat binding factor (CBF)/dehydration-responsive element binding (DREB1) proteins play a prominent role in freezing tolerance and are highly conserved in higher plants. Here we performed a genome-wide search of the
CBF/DREB1
gene family in lettuce (
Lactuca sativa
L.) and identified 14 members of the family with one member gene containing a non-sense mutation within the AP2 DNA-binding domain. A comprehensive phylogenetic analysis of the
CBF/DREB1
family members in 20 plant species from the Asterid or Rosid clade provided evidence that tandem duplication played an important role in the expansion of the
CBF/DREB1
family. Expression analysis showed that twelve of the lettuce
CBF
genes were responsive to low temperature (4 °C), and that three and six of them could also be responsive to salt and heat stresses, respectively. Unlike
Arabidopsis thaliana
whose members of the
CBF/DREB1
family respond only to a particular stress, lettuce CBFs provide wider protection from combinations of abiotic stresses. A global transcriptome analysis revealed distinctive temporal expression patterns among the cold-regulated genes in lettuce plants exposed to low temperature. Genes induced throughout the cold treatment are enriched in functions associated with protection from UV and high-light intensity and the genes suppressed after 7 days of cold exposure are enriched in photosynthesis-associated functions. These results provide insight into the molecular evolutionary properties of the
CBF/DREB1
gene family in lettuce and a reference for genetic improvement of the lettuce response to cold acclimation. |
| doi_str_mv | 10.1038/s41598-020-62458-1 |
| format | Article |
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CBF/DREB1
gene family in lettuce (
Lactuca sativa
L.) and identified 14 members of the family with one member gene containing a non-sense mutation within the AP2 DNA-binding domain. A comprehensive phylogenetic analysis of the
CBF/DREB1
family members in 20 plant species from the Asterid or Rosid clade provided evidence that tandem duplication played an important role in the expansion of the
CBF/DREB1
family. Expression analysis showed that twelve of the lettuce
CBF
genes were responsive to low temperature (4 °C), and that three and six of them could also be responsive to salt and heat stresses, respectively. Unlike
Arabidopsis thaliana
whose members of the
CBF/DREB1
family respond only to a particular stress, lettuce CBFs provide wider protection from combinations of abiotic stresses. A global transcriptome analysis revealed distinctive temporal expression patterns among the cold-regulated genes in lettuce plants exposed to low temperature. Genes induced throughout the cold treatment are enriched in functions associated with protection from UV and high-light intensity and the genes suppressed after 7 days of cold exposure are enriched in photosynthesis-associated functions. These results provide insight into the molecular evolutionary properties of the
CBF/DREB1
gene family in lettuce and a reference for genetic improvement of the lettuce response to cold acclimation.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-020-62458-1</identifier><identifier>PMID: 32235838</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>38 ; 38/39 ; 38/77 ; 45 ; 45/23 ; 45/91 ; 631/114/2403 ; 631/114/739 ; 631/449/2661/2665 ; Acclimation ; Acclimatization ; Acclimatization - genetics ; CBF protein ; Cold ; Cold acclimation ; Cold tolerance ; Dehydration ; Freezing ; Gene Expression ; Gene Expression Regulation, Plant ; Genes ; Genomes ; Heat tolerance ; Humanities and Social Sciences ; Lactuca - genetics ; Lettuce ; Light intensity ; Low temperature ; multidisciplinary ; Photosynthesis ; Phylogeny ; Plant Proteins - genetics ; Plant species ; Regulatory sequences ; Science ; Science (multidisciplinary) ; Transcription Factors - genetics ; Ultraviolet radiation ; Vegetables</subject><ispartof>Scientific reports, 2020-03, Vol.10 (1), p.5733-5733, Article 5733</ispartof><rights>The Author(s) 2020</rights><rights>This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c511t-942eb50180b53c901c8c32aaf9c0e6b0221bfc6393bddaefe138356bfe29c0b53</citedby><cites>FETCH-LOGICAL-c511t-942eb50180b53c901c8c32aaf9c0e6b0221bfc6393bddaefe138356bfe29c0b53</cites><orcidid>0000-0002-7398-9476</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7109083/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7109083/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27915,27916,41111,42180,51567,53782,53784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32235838$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Park, Sunchung</creatorcontrib><creatorcontrib>Shi, Ainong</creatorcontrib><creatorcontrib>Mou, Beiquan</creatorcontrib><title>Genome-wide identification and expression analysis of the CBF/DREB1 gene family in lettuce</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>The C-repeat binding factor (CBF)/dehydration-responsive element binding (DREB1) proteins play a prominent role in freezing tolerance and are highly conserved in higher plants. Here we performed a genome-wide search of the
CBF/DREB1
gene family in lettuce (
Lactuca sativa
L.) and identified 14 members of the family with one member gene containing a non-sense mutation within the AP2 DNA-binding domain. A comprehensive phylogenetic analysis of the
CBF/DREB1
family members in 20 plant species from the Asterid or Rosid clade provided evidence that tandem duplication played an important role in the expansion of the
CBF/DREB1
family. Expression analysis showed that twelve of the lettuce
CBF
genes were responsive to low temperature (4 °C), and that three and six of them could also be responsive to salt and heat stresses, respectively. Unlike
Arabidopsis thaliana
whose members of the
CBF/DREB1
family respond only to a particular stress, lettuce CBFs provide wider protection from combinations of abiotic stresses. A global transcriptome analysis revealed distinctive temporal expression patterns among the cold-regulated genes in lettuce plants exposed to low temperature. Genes induced throughout the cold treatment are enriched in functions associated with protection from UV and high-light intensity and the genes suppressed after 7 days of cold exposure are enriched in photosynthesis-associated functions. These results provide insight into the molecular evolutionary properties of the
CBF/DREB1
gene family in lettuce and a reference for genetic improvement of the lettuce response to cold acclimation.</description><subject>38</subject><subject>38/39</subject><subject>38/77</subject><subject>45</subject><subject>45/23</subject><subject>45/91</subject><subject>631/114/2403</subject><subject>631/114/739</subject><subject>631/449/2661/2665</subject><subject>Acclimation</subject><subject>Acclimatization</subject><subject>Acclimatization - genetics</subject><subject>CBF protein</subject><subject>Cold</subject><subject>Cold acclimation</subject><subject>Cold tolerance</subject><subject>Dehydration</subject><subject>Freezing</subject><subject>Gene Expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>Genomes</subject><subject>Heat tolerance</subject><subject>Humanities and Social Sciences</subject><subject>Lactuca - genetics</subject><subject>Lettuce</subject><subject>Light intensity</subject><subject>Low temperature</subject><subject>multidisciplinary</subject><subject>Photosynthesis</subject><subject>Phylogeny</subject><subject>Plant Proteins - genetics</subject><subject>Plant species</subject><subject>Regulatory sequences</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Transcription Factors - genetics</subject><subject>Ultraviolet radiation</subject><subject>Vegetables</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9UU1vGyEQRVWrOnLzB3qokHLpZRs-Fpu9REqcT8lSpaq95IJYdrCxdsGB3ST-9yXZJE1yKBIMMO89ZngIfaXkByVcHqaSikoWhJFixkohC_oB7TFSioJxxj6-2k_QfkobkodgVUmrz2iSb7mQXO6h6wvwoYPizjWA8_S9s87o3gWPtW8w3G8jpDQedbtLLuFgcb8GvDg5Pzz9dXZC8Qo8YKs71-6w87iFvh8MfEGfrG4T7D_FKfpzfvZ7cVksf15cLY6XhRGU9kVVMqgFoZLUgpuKUCMNZ1rbyhCY1YQxWlsz4xWvm0aDBcolF7PaAsuIzJmio1F3O9QdNCb3EHWrttF1Ou5U0E69zXi3Vqtwq-aUVETyLPD9SSCGmwFSrzqXDLSt9hCGpBiXYs45zcsUHbyDbsIQ88c8okpJyjl5qIiNKBNDShHsSzGUqAf31Oieyu6pR_cUzaRvr9t4oTx7lQF8BKSc8iuI_97-j-xfyJClNw</recordid><startdate>20200331</startdate><enddate>20200331</enddate><creator>Park, Sunchung</creator><creator>Shi, Ainong</creator><creator>Mou, Beiquan</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-7398-9476</orcidid></search><sort><creationdate>20200331</creationdate><title>Genome-wide identification and expression analysis of the CBF/DREB1 gene family in lettuce</title><author>Park, Sunchung ; Shi, Ainong ; Mou, Beiquan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c511t-942eb50180b53c901c8c32aaf9c0e6b0221bfc6393bddaefe138356bfe29c0b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>38</topic><topic>38/39</topic><topic>38/77</topic><topic>45</topic><topic>45/23</topic><topic>45/91</topic><topic>631/114/2403</topic><topic>631/114/739</topic><topic>631/449/2661/2665</topic><topic>Acclimation</topic><topic>Acclimatization</topic><topic>Acclimatization - genetics</topic><topic>CBF protein</topic><topic>Cold</topic><topic>Cold acclimation</topic><topic>Cold tolerance</topic><topic>Dehydration</topic><topic>Freezing</topic><topic>Gene Expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes</topic><topic>Genomes</topic><topic>Heat tolerance</topic><topic>Humanities and Social Sciences</topic><topic>Lactuca - genetics</topic><topic>Lettuce</topic><topic>Light intensity</topic><topic>Low temperature</topic><topic>multidisciplinary</topic><topic>Photosynthesis</topic><topic>Phylogeny</topic><topic>Plant Proteins - genetics</topic><topic>Plant species</topic><topic>Regulatory sequences</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Transcription Factors - genetics</topic><topic>Ultraviolet radiation</topic><topic>Vegetables</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, Sunchung</creatorcontrib><creatorcontrib>Shi, Ainong</creatorcontrib><creatorcontrib>Mou, Beiquan</creatorcontrib><collection>Springer Nature OA/Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, Sunchung</au><au>Shi, Ainong</au><au>Mou, Beiquan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genome-wide identification and expression analysis of the CBF/DREB1 gene family in lettuce</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2020-03-31</date><risdate>2020</risdate><volume>10</volume><issue>1</issue><spage>5733</spage><epage>5733</epage><pages>5733-5733</pages><artnum>5733</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>The C-repeat binding factor (CBF)/dehydration-responsive element binding (DREB1) proteins play a prominent role in freezing tolerance and are highly conserved in higher plants. Here we performed a genome-wide search of the
CBF/DREB1
gene family in lettuce (
Lactuca sativa
L.) and identified 14 members of the family with one member gene containing a non-sense mutation within the AP2 DNA-binding domain. A comprehensive phylogenetic analysis of the
CBF/DREB1
family members in 20 plant species from the Asterid or Rosid clade provided evidence that tandem duplication played an important role in the expansion of the
CBF/DREB1
family. Expression analysis showed that twelve of the lettuce
CBF
genes were responsive to low temperature (4 °C), and that three and six of them could also be responsive to salt and heat stresses, respectively. Unlike
Arabidopsis thaliana
whose members of the
CBF/DREB1
family respond only to a particular stress, lettuce CBFs provide wider protection from combinations of abiotic stresses. A global transcriptome analysis revealed distinctive temporal expression patterns among the cold-regulated genes in lettuce plants exposed to low temperature. Genes induced throughout the cold treatment are enriched in functions associated with protection from UV and high-light intensity and the genes suppressed after 7 days of cold exposure are enriched in photosynthesis-associated functions. These results provide insight into the molecular evolutionary properties of the
CBF/DREB1
gene family in lettuce and a reference for genetic improvement of the lettuce response to cold acclimation.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>32235838</pmid><doi>10.1038/s41598-020-62458-1</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-7398-9476</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Nature Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection; Springer Nature OA/Free Journals; Free Full-Text Journals in Chemistry |
| subjects | 38 38/39 38/77 45 45/23 45/91 631/114/2403 631/114/739 631/449/2661/2665 Acclimation Acclimatization Acclimatization - genetics CBF protein Cold Cold acclimation Cold tolerance Dehydration Freezing Gene Expression Gene Expression Regulation, Plant Genes Genomes Heat tolerance Humanities and Social Sciences Lactuca - genetics Lettuce Light intensity Low temperature multidisciplinary Photosynthesis Phylogeny Plant Proteins - genetics Plant species Regulatory sequences Science Science (multidisciplinary) Transcription Factors - genetics Ultraviolet radiation Vegetables |
| title | Genome-wide identification and expression analysis of the CBF/DREB1 gene family in lettuce |
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