Gene Expression Analysis of Human Papillomavirus-Associated Colorectal Carcinoma
Purpose. Human papillomavirus (HPV) antigens had been found in colorectal cancer (CRC) tissue, but little evidence demonstrates the association of HPV with oncogene mutations in CRC. We aim to elucidate the mutated genes that link HPV infection and CRC carcinogenesis. Methods. Cancerous and adjacent...
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| creator | Xie, Yanxuan Liu, Kaixi Chen, Xiang Jiao, Xiaoyang Chen, Jinghong Yuan, Yumeng Sun, Jiayu Shen, Wenjun Yao, Fen Fan, Zhiqiang Li, Yazhen Qiu, Qiancheng Cai, Leshan |
| description | Purpose. Human papillomavirus (HPV) antigens had been found in colorectal cancer (CRC) tissue, but little evidence demonstrates the association of HPV with oncogene mutations in CRC. We aim to elucidate the mutated genes that link HPV infection and CRC carcinogenesis. Methods. Cancerous and adjacent noncancerous tissues were obtained from CRC patients. HPV antigen was measured by using the immunohistochemical (IHC) technique. The differentially expressed genes (DEGs) in HPV-positive and HPV-negative tumor tissues were measured by using TaqMan Array Plates. The target genes were validated with the qPCR method. Results. 15 (31.9%) cases of CRC patients were observed to be HPV positive, in which HPV antigen was expressed in most tumor tissues rather than in adjacent noncancerous tissues. With TaqMan Array Plates analyses, we found that 39 differentially expressed genes (DEGs) were upregulated, while 17 DEGs were downregulated in HPV-positive CRC tissues compared with HPV-negative tissues. Four DEGs (MMP-7, MYC, WNT-5A, and AXIN2) were upregulated in tumor vs. normal tissues, or adenoma vs. normal tissue in TCGA, which was overlapped with our data. In the confirmation test, MMP-7, MYC, WNT-5A, and AXIN2 were upregulated in cancerous tissue compared with adjacent noncancerous tissue. MYC, WNT-5A, and AXIN2 were shown to be upregulated in HPV-positive CRC tissues when compared to HPV-negative tissues. Conclusion. HPV-encoding genome may integrate into the tumor genomes that involved in multiple signaling pathways. Further genomic and proteomic investigation is necessary for obtaining a more comprehensive knowledge of signaling pathways associated with the CRC carcinogenesis. |
| doi_str_mv | 10.1155/2020/5201587 |
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Human papillomavirus (HPV) antigens had been found in colorectal cancer (CRC) tissue, but little evidence demonstrates the association of HPV with oncogene mutations in CRC. We aim to elucidate the mutated genes that link HPV infection and CRC carcinogenesis. Methods. Cancerous and adjacent noncancerous tissues were obtained from CRC patients. HPV antigen was measured by using the immunohistochemical (IHC) technique. The differentially expressed genes (DEGs) in HPV-positive and HPV-negative tumor tissues were measured by using TaqMan Array Plates. The target genes were validated with the qPCR method. Results. 15 (31.9%) cases of CRC patients were observed to be HPV positive, in which HPV antigen was expressed in most tumor tissues rather than in adjacent noncancerous tissues. With TaqMan Array Plates analyses, we found that 39 differentially expressed genes (DEGs) were upregulated, while 17 DEGs were downregulated in HPV-positive CRC tissues compared with HPV-negative tissues. Four DEGs (MMP-7, MYC, WNT-5A, and AXIN2) were upregulated in tumor vs. normal tissues, or adenoma vs. normal tissue in TCGA, which was overlapped with our data. In the confirmation test, MMP-7, MYC, WNT-5A, and AXIN2 were upregulated in cancerous tissue compared with adjacent noncancerous tissue. MYC, WNT-5A, and AXIN2 were shown to be upregulated in HPV-positive CRC tissues when compared to HPV-negative tissues. Conclusion. HPV-encoding genome may integrate into the tumor genomes that involved in multiple signaling pathways. Further genomic and proteomic investigation is necessary for obtaining a more comprehensive knowledge of signaling pathways associated with the CRC carcinogenesis.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2020/5201587</identifier><identifier>PMID: 32258125</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Adenoma ; Adult ; Aged ; Aged, 80 and over ; Antigens ; Arrays ; Bioinformatics ; Cancer ; Carcinogenesis ; Carcinogens ; Colorectal cancer ; Colorectal carcinoma ; Colorectal Neoplasms - metabolism ; Colorectal Neoplasms - pathology ; Colorectal Neoplasms - virology ; Deoxyribonucleic acid ; Development and progression ; DNA ; Ethics ; Female ; Gene expression ; Gene Expression Regulation, Neoplastic ; Gene mutations ; Genes ; Genetic aspects ; Genomes ; Genomics ; Health aspects ; Human papillomavirus ; Humans ; Infections ; Male ; Matrilysin ; Metastasis ; Middle Aged ; Mutation ; Myc protein ; Neoplasm Proteins - biosynthesis ; Papillomaviridae - metabolism ; Papillomavirus infections ; Papillomavirus Infections - metabolism ; Papillomavirus Infections - pathology ; Papillomaviruses ; Pathogenesis ; Patients ; Plates ; Proteomics ; Sample size ; Signal transduction ; Signaling ; Software ; Tissues ; Tumors ; Wnt protein</subject><ispartof>BioMed research international, 2020, Vol.2020 (2020), p.1-14</ispartof><rights>Copyright © 2020 Qiancheng Qiu et al.</rights><rights>COPYRIGHT 2020 John Wiley & Sons, Inc.</rights><rights>Copyright © 2020 Qiancheng Qiu et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. http://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2020 Qiancheng Qiu et al. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-b8c12995b78d932b41e05fb1da2e71785bba2844de0868eccc4467c8a21e0f073</citedby><cites>FETCH-LOGICAL-c499t-b8c12995b78d932b41e05fb1da2e71785bba2844de0868eccc4467c8a21e0f073</cites><orcidid>0000-0002-7568-0595 ; 0000-0002-1764-4748</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7103040/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7103040/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,4021,27921,27922,27923,53789,53791</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32258125$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Maruyama, Satoshi</contributor><contributor>Satoshi Maruyama</contributor><creatorcontrib>Xie, Yanxuan</creatorcontrib><creatorcontrib>Liu, Kaixi</creatorcontrib><creatorcontrib>Chen, Xiang</creatorcontrib><creatorcontrib>Jiao, Xiaoyang</creatorcontrib><creatorcontrib>Chen, Jinghong</creatorcontrib><creatorcontrib>Yuan, Yumeng</creatorcontrib><creatorcontrib>Sun, Jiayu</creatorcontrib><creatorcontrib>Shen, Wenjun</creatorcontrib><creatorcontrib>Yao, Fen</creatorcontrib><creatorcontrib>Fan, Zhiqiang</creatorcontrib><creatorcontrib>Li, Yazhen</creatorcontrib><creatorcontrib>Qiu, Qiancheng</creatorcontrib><creatorcontrib>Cai, Leshan</creatorcontrib><title>Gene Expression Analysis of Human Papillomavirus-Associated Colorectal Carcinoma</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>Purpose. Human papillomavirus (HPV) antigens had been found in colorectal cancer (CRC) tissue, but little evidence demonstrates the association of HPV with oncogene mutations in CRC. We aim to elucidate the mutated genes that link HPV infection and CRC carcinogenesis. Methods. Cancerous and adjacent noncancerous tissues were obtained from CRC patients. HPV antigen was measured by using the immunohistochemical (IHC) technique. The differentially expressed genes (DEGs) in HPV-positive and HPV-negative tumor tissues were measured by using TaqMan Array Plates. The target genes were validated with the qPCR method. Results. 15 (31.9%) cases of CRC patients were observed to be HPV positive, in which HPV antigen was expressed in most tumor tissues rather than in adjacent noncancerous tissues. With TaqMan Array Plates analyses, we found that 39 differentially expressed genes (DEGs) were upregulated, while 17 DEGs were downregulated in HPV-positive CRC tissues compared with HPV-negative tissues. Four DEGs (MMP-7, MYC, WNT-5A, and AXIN2) were upregulated in tumor vs. normal tissues, or adenoma vs. normal tissue in TCGA, which was overlapped with our data. In the confirmation test, MMP-7, MYC, WNT-5A, and AXIN2 were upregulated in cancerous tissue compared with adjacent noncancerous tissue. MYC, WNT-5A, and AXIN2 were shown to be upregulated in HPV-positive CRC tissues when compared to HPV-negative tissues. Conclusion. HPV-encoding genome may integrate into the tumor genomes that involved in multiple signaling pathways. Further genomic and proteomic investigation is necessary for obtaining a more comprehensive knowledge of signaling pathways associated with the CRC carcinogenesis.</description><subject>Adenoma</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Antigens</subject><subject>Arrays</subject><subject>Bioinformatics</subject><subject>Cancer</subject><subject>Carcinogenesis</subject><subject>Carcinogens</subject><subject>Colorectal cancer</subject><subject>Colorectal carcinoma</subject><subject>Colorectal Neoplasms - metabolism</subject><subject>Colorectal Neoplasms - pathology</subject><subject>Colorectal Neoplasms - virology</subject><subject>Deoxyribonucleic acid</subject><subject>Development and progression</subject><subject>DNA</subject><subject>Ethics</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Gene mutations</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Health aspects</subject><subject>Human papillomavirus</subject><subject>Humans</subject><subject>Infections</subject><subject>Male</subject><subject>Matrilysin</subject><subject>Metastasis</subject><subject>Middle Aged</subject><subject>Mutation</subject><subject>Myc protein</subject><subject>Neoplasm Proteins - biosynthesis</subject><subject>Papillomaviridae - metabolism</subject><subject>Papillomavirus infections</subject><subject>Papillomavirus Infections - metabolism</subject><subject>Papillomavirus Infections - pathology</subject><subject>Papillomaviruses</subject><subject>Pathogenesis</subject><subject>Patients</subject><subject>Plates</subject><subject>Proteomics</subject><subject>Sample size</subject><subject>Signal transduction</subject><subject>Signaling</subject><subject>Software</subject><subject>Tissues</subject><subject>Tumors</subject><subject>Wnt protein</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqN0U1LHTEUBuAgLVWsu65lwGU7mpOPmcxGuFz8AqEudB0ymTMamUlukxmt_97IvV7rrtkkkIeXc3gJ-QH0GEDKE0YZPZGMglT1DtljHERZgYAv2zfnu-QgpUeaj4KKNtU3sssZkwqY3CM3F-ixOPu7ipiSC75YeDO8JJeK0BeX82h8cWNWbhjCaJ5cnFO5SClYZybsimUYQkQ7maFYmmidz-g7-dqbIeHB5t4nd-dnt8vL8vr3xdVycV1a0TRT2SoLrGlkW6uu4awVgFT2LXSGYQ21km1rmBKiQ6oqhdZaIaraKsMy7GnN98npOnc1tyN2Fv0UzaBX0Y0mvuhgnP78492Dvg9PugbKqaA54GgTEMOfGdOkH8Mc8_ZJM64EcFmD_FD3ZkDtfB9ymB1dsnpRMVGpJodl9WutbAwpRey3cwDVb0Xpt6L0pqjMD_-dfYvfa8ng5xo8ON-ZZ_efcZgN9uZDAxeyUvwV2q6j2A</recordid><startdate>2020</startdate><enddate>2020</enddate><creator>Xie, Yanxuan</creator><creator>Liu, Kaixi</creator><creator>Chen, Xiang</creator><creator>Jiao, Xiaoyang</creator><creator>Chen, Jinghong</creator><creator>Yuan, Yumeng</creator><creator>Sun, Jiayu</creator><creator>Shen, Wenjun</creator><creator>Yao, Fen</creator><creator>Fan, Zhiqiang</creator><creator>Li, Yazhen</creator><creator>Qiu, Qiancheng</creator><creator>Cai, Leshan</creator><general>Hindawi Publishing Corporation</general><general>Hindawi</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-7568-0595</orcidid><orcidid>https://orcid.org/0000-0002-1764-4748</orcidid></search><sort><creationdate>2020</creationdate><title>Gene Expression Analysis of Human Papillomavirus-Associated Colorectal Carcinoma</title><author>Xie, Yanxuan ; Liu, Kaixi ; Chen, Xiang ; Jiao, Xiaoyang ; Chen, Jinghong ; Yuan, Yumeng ; Sun, Jiayu ; Shen, Wenjun ; Yao, Fen ; Fan, Zhiqiang ; Li, Yazhen ; Qiu, Qiancheng ; Cai, Leshan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-b8c12995b78d932b41e05fb1da2e71785bba2844de0868eccc4467c8a21e0f073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adenoma</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Antigens</topic><topic>Arrays</topic><topic>Bioinformatics</topic><topic>Cancer</topic><topic>Carcinogenesis</topic><topic>Carcinogens</topic><topic>Colorectal cancer</topic><topic>Colorectal carcinoma</topic><topic>Colorectal Neoplasms - metabolism</topic><topic>Colorectal Neoplasms - pathology</topic><topic>Colorectal Neoplasms - virology</topic><topic>Deoxyribonucleic acid</topic><topic>Development and progression</topic><topic>DNA</topic><topic>Ethics</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Gene mutations</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Health aspects</topic><topic>Human papillomavirus</topic><topic>Humans</topic><topic>Infections</topic><topic>Male</topic><topic>Matrilysin</topic><topic>Metastasis</topic><topic>Middle Aged</topic><topic>Mutation</topic><topic>Myc protein</topic><topic>Neoplasm Proteins - biosynthesis</topic><topic>Papillomaviridae - metabolism</topic><topic>Papillomavirus infections</topic><topic>Papillomavirus Infections - metabolism</topic><topic>Papillomavirus Infections - pathology</topic><topic>Papillomaviruses</topic><topic>Pathogenesis</topic><topic>Patients</topic><topic>Plates</topic><topic>Proteomics</topic><topic>Sample size</topic><topic>Signal transduction</topic><topic>Signaling</topic><topic>Software</topic><topic>Tissues</topic><topic>Tumors</topic><topic>Wnt protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xie, Yanxuan</creatorcontrib><creatorcontrib>Liu, Kaixi</creatorcontrib><creatorcontrib>Chen, Xiang</creatorcontrib><creatorcontrib>Jiao, Xiaoyang</creatorcontrib><creatorcontrib>Chen, Jinghong</creatorcontrib><creatorcontrib>Yuan, Yumeng</creatorcontrib><creatorcontrib>Sun, Jiayu</creatorcontrib><creatorcontrib>Shen, Wenjun</creatorcontrib><creatorcontrib>Yao, Fen</creatorcontrib><creatorcontrib>Fan, Zhiqiang</creatorcontrib><creatorcontrib>Li, Yazhen</creatorcontrib><creatorcontrib>Qiu, Qiancheng</creatorcontrib><creatorcontrib>Cai, Leshan</creatorcontrib><collection>الدوريات العلمية والإحصائية - 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Human papillomavirus (HPV) antigens had been found in colorectal cancer (CRC) tissue, but little evidence demonstrates the association of HPV with oncogene mutations in CRC. We aim to elucidate the mutated genes that link HPV infection and CRC carcinogenesis. Methods. Cancerous and adjacent noncancerous tissues were obtained from CRC patients. HPV antigen was measured by using the immunohistochemical (IHC) technique. The differentially expressed genes (DEGs) in HPV-positive and HPV-negative tumor tissues were measured by using TaqMan Array Plates. The target genes were validated with the qPCR method. Results. 15 (31.9%) cases of CRC patients were observed to be HPV positive, in which HPV antigen was expressed in most tumor tissues rather than in adjacent noncancerous tissues. With TaqMan Array Plates analyses, we found that 39 differentially expressed genes (DEGs) were upregulated, while 17 DEGs were downregulated in HPV-positive CRC tissues compared with HPV-negative tissues. Four DEGs (MMP-7, MYC, WNT-5A, and AXIN2) were upregulated in tumor vs. normal tissues, or adenoma vs. normal tissue in TCGA, which was overlapped with our data. In the confirmation test, MMP-7, MYC, WNT-5A, and AXIN2 were upregulated in cancerous tissue compared with adjacent noncancerous tissue. MYC, WNT-5A, and AXIN2 were shown to be upregulated in HPV-positive CRC tissues when compared to HPV-negative tissues. Conclusion. HPV-encoding genome may integrate into the tumor genomes that involved in multiple signaling pathways. Further genomic and proteomic investigation is necessary for obtaining a more comprehensive knowledge of signaling pathways associated with the CRC carcinogenesis.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>32258125</pmid><doi>10.1155/2020/5201587</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-7568-0595</orcidid><orcidid>https://orcid.org/0000-0002-1764-4748</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adenoma Adult Aged Aged, 80 and over Antigens Arrays Bioinformatics Cancer Carcinogenesis Carcinogens Colorectal cancer Colorectal carcinoma Colorectal Neoplasms - metabolism Colorectal Neoplasms - pathology Colorectal Neoplasms - virology Deoxyribonucleic acid Development and progression DNA Ethics Female Gene expression Gene Expression Regulation, Neoplastic Gene mutations Genes Genetic aspects Genomes Genomics Health aspects Human papillomavirus Humans Infections Male Matrilysin Metastasis Middle Aged Mutation Myc protein Neoplasm Proteins - biosynthesis Papillomaviridae - metabolism Papillomavirus infections Papillomavirus Infections - metabolism Papillomavirus Infections - pathology Papillomaviruses Pathogenesis Patients Plates Proteomics Sample size Signal transduction Signaling Software Tissues Tumors Wnt protein |
| title | Gene Expression Analysis of Human Papillomavirus-Associated Colorectal Carcinoma |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T16%3A30%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Gene%20Expression%20Analysis%20of%20Human%20Papillomavirus-Associated%20Colorectal%20Carcinoma&rft.jtitle=BioMed%20research%20international&rft.au=Xie,%20Yanxuan&rft.date=2020&rft.volume=2020&rft.issue=2020&rft.spage=1&rft.epage=14&rft.pages=1-14&rft.issn=2314-6133&rft.eissn=2314-6141&rft_id=info:doi/10.1155/2020/5201587&rft_dat=%3Cgale_pubme%3EA624689304%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2384135715&rft_id=info:pmid/32258125&rft_galeid=A624689304&rfr_iscdi=true |