Intestinal microbiota characteristics of mice treated with Folium senna decoction gavage combined with restraint and tail pinch stress
To investigate the characteristics of the intestinal microbiota of mice treated with Folium senna decoction gavage combined with restraint and tail pinch stress. Ten healthy male Kunming mice were chosen and randomly divided into control group and model group, with five mice in each group. Mice in t...
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| creator | Yuan, Zhen Zhang, Chenyang Peng, Xinxin Shu, Lan Long, Chengxing Tan, Zhoujin |
| description | To investigate the characteristics of the intestinal microbiota of mice treated with
Folium senna
decoction gavage combined with restraint and tail pinch stress. Ten healthy male Kunming mice were chosen and randomly divided into control group and model group, with five mice in each group. Mice in the control group were raised regularly, while mice in the model group were treated by feeding with
Folium senna
decoction, restraint in a constraint tube and tail pinch with a clip for 7 days. Intestinal contents from the jejunum to ileum were collected, and DNA was extracted from each mouse. The characteristics of the intestinal microbial species were analysed by PacBio Sequel-based 16S rRNA sequencing. Result showed that alpha diversity indices in the model group were higher than those in the control group, and the Simpson index differed significantly (
P
0.05). In the control group,
Candidatus arthromitus
sp
.
SFB-mouse and
Lactobacillus johnsonii
were the dominant species. In the model group,
Staphylococcus lentus
,
Lactobacillus johnsonii
,
Candidatus arthromitus
sp. SFB-mouse and
Lactobacillus murinus
were included. Furthermore, LEfSe analysis showed that the relative abundances of
Escherichia
sp. BBDP27,
Helicobacter ganmani
,
Bacteroides vulgatus
and
Lactobacillus intestinalis
in the model group were higher than those in the control group (
P
|
| doi_str_mv | 10.1007/s13205-020-02172-x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7101465</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2431845174</sourcerecordid><originalsourceid>FETCH-LOGICAL-c507t-e6f4d3d0b98d4a36df673426a6daeea53488953fccbb6a4d79448dcbb64419563</originalsourceid><addsrcrecordid>eNqFkktrFTEUxwdRbGn7BVxIwI2b0bwzsxGkWC0U3LTQXTiTZO5NmUmuSaatX8DPbW5ve30sNBDyOL_z4vyb5hXB7wjG6n0mjGLRYorrJoq298-aQ0p63ArFuuf7O70-aE5yvsF1CSJ6gl82B4xSRnpJDpsf56G4XHyACc3epDj4WACZNSQwxSVfbSajOG6tDpXkoDiL7nxZo7M4-WVG2YUAyDoTTfExoBXcwsohE-fBhyc21SQJfCgIgkUF_IQ2Ppg1qt8u5-PmxQhTdieP51Fzdfbp8vRLe_H18_npx4vWCKxK6-TILbN46DvLgUk7SsU4lSAtOAeC8a7rBRuNGQYJ3Kqe885uH5yTXkh21HzYxd0sw-yscaFWNelN8jOk7zqC139agl_rVbzVimDCpagB3j4GSPHbUpvSs8_GTRMEF5esKWek44Io_n-UdYJ2WClc0Td_oTdxSXUmDxTriKzpK0V3VB1TzsmN-7oJ1ltR6J0odBWFfhCFvq9Or3_veO_yJIEKsB2QqymsXPqV-x9hfwKQasXm</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2383816101</pqid></control><display><type>article</type><title>Intestinal microbiota characteristics of mice treated with Folium senna decoction gavage combined with restraint and tail pinch stress</title><source>SpringerLink Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>Yuan, Zhen ; Zhang, Chenyang ; Peng, Xinxin ; Shu, Lan ; Long, Chengxing ; Tan, Zhoujin</creator><creatorcontrib>Yuan, Zhen ; Zhang, Chenyang ; Peng, Xinxin ; Shu, Lan ; Long, Chengxing ; Tan, Zhoujin</creatorcontrib><description>To investigate the characteristics of the intestinal microbiota of mice treated with
Folium senna
decoction gavage combined with restraint and tail pinch stress. Ten healthy male Kunming mice were chosen and randomly divided into control group and model group, with five mice in each group. Mice in the control group were raised regularly, while mice in the model group were treated by feeding with
Folium senna
decoction, restraint in a constraint tube and tail pinch with a clip for 7 days. Intestinal contents from the jejunum to ileum were collected, and DNA was extracted from each mouse. The characteristics of the intestinal microbial species were analysed by PacBio Sequel-based 16S rRNA sequencing. Result showed that alpha diversity indices in the model group were higher than those in the control group, and the Simpson index differed significantly (
P
< 0.05). Based on the composition and abundances of species, there were differences between the control group and model group at the species level, but these differences were not significant (
P
> 0.05). In the control group,
Candidatus arthromitus
sp
.
SFB-mouse and
Lactobacillus johnsonii
were the dominant species. In the model group,
Staphylococcus lentus
,
Lactobacillus johnsonii
,
Candidatus arthromitus
sp. SFB-mouse and
Lactobacillus murinus
were included. Furthermore, LEfSe analysis showed that the relative abundances of
Escherichia
sp. BBDP27,
Helicobacter ganmani
,
Bacteroides vulgatus
and
Lactobacillus intestinalis
in the model group were higher than those in the control group (
P
< 0.05 or
P
< 0.01). In conclusion,
Folium senna
decoction gavage combined with restraint and tail pinch stress increased the intestinal microbiota diversity. Strains associated with intestinal diseases, including
Bacteroides vulgatus
,
Helicobacter ganmani
,
Staphylococcus lentus
and
Lactobacillus murinus
, were significantly enriched, while strains beneficial to health, such as
Candidatus arthromitus
sp. SFB-mouse and
Lactobacillus johnsonii
, were significantly depleted.</description><identifier>ISSN: 2190-572X</identifier><identifier>EISSN: 2190-5738</identifier><identifier>DOI: 10.1007/s13205-020-02172-x</identifier><identifier>PMID: 32231961</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Abundance ; Agriculture ; Bacteroides ; Bacteroides vulgatus ; Bioinformatics ; Biomaterials ; Biotechnology ; Cancer Research ; Candidatus Arthromitus ; Chemistry ; Chemistry and Materials Science ; Constraints ; Diversity indices ; DNA ; Dominant species ; Escherichia ; Food additives ; gastrointestinal diseases ; Helicobacter ; Herbal medicine ; Ileum ; Intestinal microflora ; intestinal microorganisms ; Jejunum ; Lactobacillus ; Lactobacillus intestinalis ; Lactobacillus johnsonii ; Lactobacillus murinus ; males ; mice ; Microbiota ; Microorganisms ; Original ; Original Article ; ribosomal RNA ; rRNA 16S ; sequence analysis ; species diversity ; Staphylococcus ; Staphylococcus lentus ; Stem Cells</subject><ispartof>3 Biotech, 2020-04, Vol.10 (4), p.180-180, Article 180</ispartof><rights>King Abdulaziz City for Science and Technology 2020</rights><rights>King Abdulaziz City for Science and Technology 2020.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c507t-e6f4d3d0b98d4a36df673426a6daeea53488953fccbb6a4d79448dcbb64419563</citedby><cites>FETCH-LOGICAL-c507t-e6f4d3d0b98d4a36df673426a6daeea53488953fccbb6a4d79448dcbb64419563</cites><orcidid>0000-0001-6551-510X ; 0000-0002-7294-9472 ; 0000-0003-3193-073X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7101465/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7101465/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,41469,42538,51300,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32231961$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yuan, Zhen</creatorcontrib><creatorcontrib>Zhang, Chenyang</creatorcontrib><creatorcontrib>Peng, Xinxin</creatorcontrib><creatorcontrib>Shu, Lan</creatorcontrib><creatorcontrib>Long, Chengxing</creatorcontrib><creatorcontrib>Tan, Zhoujin</creatorcontrib><title>Intestinal microbiota characteristics of mice treated with Folium senna decoction gavage combined with restraint and tail pinch stress</title><title>3 Biotech</title><addtitle>3 Biotech</addtitle><addtitle>3 Biotech</addtitle><description>To investigate the characteristics of the intestinal microbiota of mice treated with
Folium senna
decoction gavage combined with restraint and tail pinch stress. Ten healthy male Kunming mice were chosen and randomly divided into control group and model group, with five mice in each group. Mice in the control group were raised regularly, while mice in the model group were treated by feeding with
Folium senna
decoction, restraint in a constraint tube and tail pinch with a clip for 7 days. Intestinal contents from the jejunum to ileum were collected, and DNA was extracted from each mouse. The characteristics of the intestinal microbial species were analysed by PacBio Sequel-based 16S rRNA sequencing. Result showed that alpha diversity indices in the model group were higher than those in the control group, and the Simpson index differed significantly (
P
< 0.05). Based on the composition and abundances of species, there were differences between the control group and model group at the species level, but these differences were not significant (
P
> 0.05). In the control group,
Candidatus arthromitus
sp
.
SFB-mouse and
Lactobacillus johnsonii
were the dominant species. In the model group,
Staphylococcus lentus
,
Lactobacillus johnsonii
,
Candidatus arthromitus
sp. SFB-mouse and
Lactobacillus murinus
were included. Furthermore, LEfSe analysis showed that the relative abundances of
Escherichia
sp. BBDP27,
Helicobacter ganmani
,
Bacteroides vulgatus
and
Lactobacillus intestinalis
in the model group were higher than those in the control group (
P
< 0.05 or
P
< 0.01). In conclusion,
Folium senna
decoction gavage combined with restraint and tail pinch stress increased the intestinal microbiota diversity. Strains associated with intestinal diseases, including
Bacteroides vulgatus
,
Helicobacter ganmani
,
Staphylococcus lentus
and
Lactobacillus murinus
, were significantly enriched, while strains beneficial to health, such as
Candidatus arthromitus
sp. SFB-mouse and
Lactobacillus johnsonii
, were significantly depleted.</description><subject>Abundance</subject><subject>Agriculture</subject><subject>Bacteroides</subject><subject>Bacteroides vulgatus</subject><subject>Bioinformatics</subject><subject>Biomaterials</subject><subject>Biotechnology</subject><subject>Cancer Research</subject><subject>Candidatus Arthromitus</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Constraints</subject><subject>Diversity indices</subject><subject>DNA</subject><subject>Dominant species</subject><subject>Escherichia</subject><subject>Food additives</subject><subject>gastrointestinal diseases</subject><subject>Helicobacter</subject><subject>Herbal medicine</subject><subject>Ileum</subject><subject>Intestinal microflora</subject><subject>intestinal microorganisms</subject><subject>Jejunum</subject><subject>Lactobacillus</subject><subject>Lactobacillus intestinalis</subject><subject>Lactobacillus johnsonii</subject><subject>Lactobacillus murinus</subject><subject>males</subject><subject>mice</subject><subject>Microbiota</subject><subject>Microorganisms</subject><subject>Original</subject><subject>Original Article</subject><subject>ribosomal RNA</subject><subject>rRNA 16S</subject><subject>sequence analysis</subject><subject>species diversity</subject><subject>Staphylococcus</subject><subject>Staphylococcus lentus</subject><subject>Stem Cells</subject><issn>2190-572X</issn><issn>2190-5738</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNqFkktrFTEUxwdRbGn7BVxIwI2b0bwzsxGkWC0U3LTQXTiTZO5NmUmuSaatX8DPbW5ve30sNBDyOL_z4vyb5hXB7wjG6n0mjGLRYorrJoq298-aQ0p63ArFuuf7O70-aE5yvsF1CSJ6gl82B4xSRnpJDpsf56G4XHyACc3epDj4WACZNSQwxSVfbSajOG6tDpXkoDiL7nxZo7M4-WVG2YUAyDoTTfExoBXcwsohE-fBhyc21SQJfCgIgkUF_IQ2Ppg1qt8u5-PmxQhTdieP51Fzdfbp8vRLe_H18_npx4vWCKxK6-TILbN46DvLgUk7SsU4lSAtOAeC8a7rBRuNGQYJ3Kqe885uH5yTXkh21HzYxd0sw-yscaFWNelN8jOk7zqC139agl_rVbzVimDCpagB3j4GSPHbUpvSs8_GTRMEF5esKWek44Io_n-UdYJ2WClc0Td_oTdxSXUmDxTriKzpK0V3VB1TzsmN-7oJ1ltR6J0odBWFfhCFvq9Or3_veO_yJIEKsB2QqymsXPqV-x9hfwKQasXm</recordid><startdate>20200401</startdate><enddate>20200401</enddate><creator>Yuan, Zhen</creator><creator>Zhang, Chenyang</creator><creator>Peng, Xinxin</creator><creator>Shu, Lan</creator><creator>Long, Chengxing</creator><creator>Tan, Zhoujin</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-6551-510X</orcidid><orcidid>https://orcid.org/0000-0002-7294-9472</orcidid><orcidid>https://orcid.org/0000-0003-3193-073X</orcidid></search><sort><creationdate>20200401</creationdate><title>Intestinal microbiota characteristics of mice treated with Folium senna decoction gavage combined with restraint and tail pinch stress</title><author>Yuan, Zhen ; Zhang, Chenyang ; Peng, Xinxin ; Shu, Lan ; Long, Chengxing ; Tan, Zhoujin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c507t-e6f4d3d0b98d4a36df673426a6daeea53488953fccbb6a4d79448dcbb64419563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Abundance</topic><topic>Agriculture</topic><topic>Bacteroides</topic><topic>Bacteroides vulgatus</topic><topic>Bioinformatics</topic><topic>Biomaterials</topic><topic>Biotechnology</topic><topic>Cancer Research</topic><topic>Candidatus Arthromitus</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Constraints</topic><topic>Diversity indices</topic><topic>DNA</topic><topic>Dominant species</topic><topic>Escherichia</topic><topic>Food additives</topic><topic>gastrointestinal diseases</topic><topic>Helicobacter</topic><topic>Herbal medicine</topic><topic>Ileum</topic><topic>Intestinal microflora</topic><topic>intestinal microorganisms</topic><topic>Jejunum</topic><topic>Lactobacillus</topic><topic>Lactobacillus intestinalis</topic><topic>Lactobacillus johnsonii</topic><topic>Lactobacillus murinus</topic><topic>males</topic><topic>mice</topic><topic>Microbiota</topic><topic>Microorganisms</topic><topic>Original</topic><topic>Original Article</topic><topic>ribosomal RNA</topic><topic>rRNA 16S</topic><topic>sequence analysis</topic><topic>species diversity</topic><topic>Staphylococcus</topic><topic>Staphylococcus lentus</topic><topic>Stem Cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yuan, Zhen</creatorcontrib><creatorcontrib>Zhang, Chenyang</creatorcontrib><creatorcontrib>Peng, Xinxin</creatorcontrib><creatorcontrib>Shu, Lan</creatorcontrib><creatorcontrib>Long, Chengxing</creatorcontrib><creatorcontrib>Tan, Zhoujin</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>3 Biotech</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yuan, Zhen</au><au>Zhang, Chenyang</au><au>Peng, Xinxin</au><au>Shu, Lan</au><au>Long, Chengxing</au><au>Tan, Zhoujin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intestinal microbiota characteristics of mice treated with Folium senna decoction gavage combined with restraint and tail pinch stress</atitle><jtitle>3 Biotech</jtitle><stitle>3 Biotech</stitle><addtitle>3 Biotech</addtitle><date>2020-04-01</date><risdate>2020</risdate><volume>10</volume><issue>4</issue><spage>180</spage><epage>180</epage><pages>180-180</pages><artnum>180</artnum><issn>2190-572X</issn><eissn>2190-5738</eissn><abstract>To investigate the characteristics of the intestinal microbiota of mice treated with
Folium senna
decoction gavage combined with restraint and tail pinch stress. Ten healthy male Kunming mice were chosen and randomly divided into control group and model group, with five mice in each group. Mice in the control group were raised regularly, while mice in the model group were treated by feeding with
Folium senna
decoction, restraint in a constraint tube and tail pinch with a clip for 7 days. Intestinal contents from the jejunum to ileum were collected, and DNA was extracted from each mouse. The characteristics of the intestinal microbial species were analysed by PacBio Sequel-based 16S rRNA sequencing. Result showed that alpha diversity indices in the model group were higher than those in the control group, and the Simpson index differed significantly (
P
< 0.05). Based on the composition and abundances of species, there were differences between the control group and model group at the species level, but these differences were not significant (
P
> 0.05). In the control group,
Candidatus arthromitus
sp
.
SFB-mouse and
Lactobacillus johnsonii
were the dominant species. In the model group,
Staphylococcus lentus
,
Lactobacillus johnsonii
,
Candidatus arthromitus
sp. SFB-mouse and
Lactobacillus murinus
were included. Furthermore, LEfSe analysis showed that the relative abundances of
Escherichia
sp. BBDP27,
Helicobacter ganmani
,
Bacteroides vulgatus
and
Lactobacillus intestinalis
in the model group were higher than those in the control group (
P
< 0.05 or
P
< 0.01). In conclusion,
Folium senna
decoction gavage combined with restraint and tail pinch stress increased the intestinal microbiota diversity. Strains associated with intestinal diseases, including
Bacteroides vulgatus
,
Helicobacter ganmani
,
Staphylococcus lentus
and
Lactobacillus murinus
, were significantly enriched, while strains beneficial to health, such as
Candidatus arthromitus
sp. SFB-mouse and
Lactobacillus johnsonii
, were significantly depleted.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>32231961</pmid><doi>10.1007/s13205-020-02172-x</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-6551-510X</orcidid><orcidid>https://orcid.org/0000-0002-7294-9472</orcidid><orcidid>https://orcid.org/0000-0003-3193-073X</orcidid><oa>free_for_read</oa></addata></record> |
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| source | SpringerLink Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Abundance Agriculture Bacteroides Bacteroides vulgatus Bioinformatics Biomaterials Biotechnology Cancer Research Candidatus Arthromitus Chemistry Chemistry and Materials Science Constraints Diversity indices DNA Dominant species Escherichia Food additives gastrointestinal diseases Helicobacter Herbal medicine Ileum Intestinal microflora intestinal microorganisms Jejunum Lactobacillus Lactobacillus intestinalis Lactobacillus johnsonii Lactobacillus murinus males mice Microbiota Microorganisms Original Original Article ribosomal RNA rRNA 16S sequence analysis species diversity Staphylococcus Staphylococcus lentus Stem Cells |
| title | Intestinal microbiota characteristics of mice treated with Folium senna decoction gavage combined with restraint and tail pinch stress |
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