Characterisation of cyclin D1 down-regulation in coronavirus infected cells
The positive strand RNA coronavirus, infectious bronchitis virus (IBV), induces a G2/M phase arrest and reduction in the G1 and G1/S phase transition regulator cyclin D1. Quantitative real-time RT-PCR and Western blot analysis demonstrated that cyclin D1 was reduced post-transcriptionally within inf...
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| Veröffentlicht in: | FEBS letters 2007-04, Vol.581 (7), p.1275-1286 |
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| creator | Harrison, Sally M. Dove, Brian K. Rothwell, Lisa Kaiser, Pete Tarpey, Ian Brooks, Gavin Hiscox, Julian A. |
| description | The positive strand RNA coronavirus, infectious bronchitis virus (IBV), induces a G2/M phase arrest and reduction in the G1 and G1/S phase transition regulator cyclin D1. Quantitative real-time RT-PCR and Western blot analysis demonstrated that cyclin D1 was reduced post-transcriptionally within infected cells independently of the cell-cycle stage at the time of infection. Confocal microscopy revealed that cyclin D1 decreased in IBV-infected cells as infection progressed and inhibition studies indicated that a population of cyclin D1 could be targeted for degradation by a virus mediated pathway. In contrast to the SARS-coronavirus, IBV nucleocapsid protein did not interact with cyclin D1. |
| doi_str_mv | 10.1016/j.febslet.2007.02.039 |
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Quantitative real-time RT-PCR and Western blot analysis demonstrated that cyclin D1 was reduced post-transcriptionally within infected cells independently of the cell-cycle stage at the time of infection. Confocal microscopy revealed that cyclin D1 decreased in IBV-infected cells as infection progressed and inhibition studies indicated that a population of cyclin D1 could be targeted for degradation by a virus mediated pathway. In contrast to the SARS-coronavirus, IBV nucleocapsid protein did not interact with cyclin D1.</description><subject>Animals</subject><subject>Blotting, Western</subject><subject>Cell cycle</subject><subject>Chlorocebus aethiops</subject><subject>Coronavirus</subject><subject>Cyclin D1</subject><subject>Cyclin D1 - analysis</subject><subject>Cyclin D1 - genetics</subject><subject>Cyclin D1 - metabolism</subject><subject>Down-Regulation</subject><subject>IBV</subject><subject>Infectious bronchitis virus</subject><subject>Regulation</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA, Viral - analysis</subject><subject>RNA, Viral - metabolism</subject><subject>Short Communication</subject><subject>Short Communications</subject><subject>Taqman</subject><subject>Vero Cells</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU9v1DAQxS0EokvLRwDlxC1hbMfx-gKCpaWolThQzpZjT1qvsnGxk6322-MoK_6c2pNnPG-e3uhHyBsKFQXavN9WHbapx7FiALICVgFXz8iKriUved2sn5MVAK1LIRU_Ia9S2kLu11S9JCdUcqHUGlbkanNnorEjRp_M6MNQhK6wB9v7ofhCCxcehjLi7dQvw_xrQwyD2fs4pdx2mHddYbHv0xl50Zk-4evje0p-XpzfbC7L6-9fv20-XZdWNBRKw5xRqhXOMCprUCpXom6ZaWuBDc81c62wHEBIYVkjqe1MLRxvWipV4_gp-bD43k_tDp3FYYym1_fR70w86GC8_n8y-Dt9G_ZagqolZdng3dEghl8TplHvfJpPMAOGKWUdZ5TB40KqmhxfzEKxCG0MKUXs_qShoGdeequPvPTMSwPTmVfee_vvKX-3joCy4HIRPPgeD09z1Rfnn9mPGf7MHiQAAzFbfVysMLPZe4w6WY-DRedjpqhd8I-k_Q39Zb_r</recordid><startdate>20070403</startdate><enddate>20070403</enddate><creator>Harrison, Sally M.</creator><creator>Dove, Brian K.</creator><creator>Rothwell, Lisa</creator><creator>Kaiser, Pete</creator><creator>Tarpey, Ian</creator><creator>Brooks, Gavin</creator><creator>Hiscox, Julian A.</creator><general>Elsevier B.V</general><general>John Wiley and Sons Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20070403</creationdate><title>Characterisation of cyclin D1 down-regulation in coronavirus infected cells</title><author>Harrison, Sally M. ; 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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals; Wiley Online Library Free Content; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Blotting, Western Cell cycle Chlorocebus aethiops Coronavirus Cyclin D1 Cyclin D1 - analysis Cyclin D1 - genetics Cyclin D1 - metabolism Down-Regulation IBV Infectious bronchitis virus Regulation Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - analysis RNA, Messenger - metabolism RNA, Viral - analysis RNA, Viral - metabolism Short Communication Short Communications Taqman Vero Cells |
| title | Characterisation of cyclin D1 down-regulation in coronavirus infected cells |
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