DNA methylation clocks as a predictor for ageing and age estimation in naked mole-rats, Heterocephalus glaber
The naked mole-rat, (NMR), the longest-lived rodent, is of significance and interest in the study of biomarkers for ageing. Recent breakthroughs in this field have revealed 'epigenetic clocks' that are based on the temporal accumulation of DNA methylation at specific genomic sites. Here, w...
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| Veröffentlicht in: | Aging (Albany, NY.) NY.), 2020-03, Vol.12 (5), p.4394-4406 |
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| container_title | Aging (Albany, NY.) |
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| creator | Lowe, Robert Danson, Amy F Rakyan, Vardhman K Yildizoglu, Selin Saldmann, Frédéric Viltard, Melanie Friedlander, Gérard Faulkes, Chris G |
| description | The naked mole-rat,
(NMR), the longest-lived rodent, is of significance and interest in the study of biomarkers for ageing. Recent breakthroughs in this field have revealed 'epigenetic clocks' that are based on the temporal accumulation of DNA methylation at specific genomic sites. Here, we validate the hypothesis of an epigenetic clock in NMRs based on changes in methylation of targeted CpG sites. We initially analysed 51 CpGs in NMR livers spanning an age range of 39-1,144 weeks and found 23 to be significantly associated with age (p |
| doi_str_mv | 10.18632/aging.102892 |
| format | Article |
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(NMR), the longest-lived rodent, is of significance and interest in the study of biomarkers for ageing. Recent breakthroughs in this field have revealed 'epigenetic clocks' that are based on the temporal accumulation of DNA methylation at specific genomic sites. Here, we validate the hypothesis of an epigenetic clock in NMRs based on changes in methylation of targeted CpG sites. We initially analysed 51 CpGs in NMR livers spanning an age range of 39-1,144 weeks and found 23 to be significantly associated with age (p<0.05). We then built a predictor of age using these sites. To test the accuracy of this model, we analysed an additional set of liver samples, and were successfully able to predict their age with a root mean squared error of 166 weeks. We also profiled skin samples with the same age range, finding a striking correlation between their predicted age versus their actual age (R=0.93), but which was lower when compared to the liver, suggesting that skin ages slower than the liver in NMRs. Our model will enable the prediction of age in wild-caught and captive NMRs of unknown age, and will be invaluable for further mechanistic studies of mammalian ageing.</description><subject>Aging - genetics</subject><subject>Aging - metabolism</subject><subject>Animals</subject><subject>CpG Islands - genetics</subject><subject>DNA Methylation</subject><subject>Liver - metabolism</subject><subject>Mole Rats</subject><subject>Research Paper</subject><subject>Skin - metabolism</subject><issn>1945-4589</issn><issn>1945-4589</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUUtLxDAQDqL4WD16lRw9WM2raXMRxDeIXvQc0mTarabNmnQF_73RVVGYYQbmm28eH0L7lBzTWnJ2Yrp-7I4pYbVia2ibKlEWoqzV-p98C-2k9EyILEshN9EWZ5RJwsQ2Gi7uz_AA0_zdm6kPI7Y-2JeETTa8iOB6O4WI2-ymgzwJm9F9phjS1A-rnn7Eo3kBh4fgoYhmSkf4BiaIwcJibvwy4c6bBuIu2miNT7D3HWfo6ery8fymuHu4vj0_uyssr6upANeWEtraNazmUinmjKGUWcFqyklluZDOcSUrU7eNUkKVJBcaJWkjWykkn6HTFe9i2QzgLIxTNF4vYt44vutgev2_MvZz3YU3XRHFP986Q4ffBDG8LvOpeuiTBe_NCGGZNOMVpYLlB2dosYLaGFKK0P6OoUR_SaS_JNIriTL-4O9uv-gfTfgHpzOOrw</recordid><startdate>20200303</startdate><enddate>20200303</enddate><creator>Lowe, Robert</creator><creator>Danson, Amy F</creator><creator>Rakyan, Vardhman K</creator><creator>Yildizoglu, Selin</creator><creator>Saldmann, Frédéric</creator><creator>Viltard, Melanie</creator><creator>Friedlander, Gérard</creator><creator>Faulkes, Chris G</creator><general>Impact Journals</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20200303</creationdate><title>DNA methylation clocks as a predictor for ageing and age estimation in naked mole-rats, Heterocephalus glaber</title><author>Lowe, Robert ; Danson, Amy F ; Rakyan, Vardhman K ; Yildizoglu, Selin ; Saldmann, Frédéric ; Viltard, Melanie ; Friedlander, Gérard ; Faulkes, Chris G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c387t-edf56ef8db2836992daa112c4281307c346dd3967a8fb994950813b961b6f6463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Aging - genetics</topic><topic>Aging - metabolism</topic><topic>Animals</topic><topic>CpG Islands - genetics</topic><topic>DNA Methylation</topic><topic>Liver - metabolism</topic><topic>Mole Rats</topic><topic>Research Paper</topic><topic>Skin - metabolism</topic><toplevel>online_resources</toplevel><creatorcontrib>Lowe, Robert</creatorcontrib><creatorcontrib>Danson, Amy F</creatorcontrib><creatorcontrib>Rakyan, Vardhman K</creatorcontrib><creatorcontrib>Yildizoglu, Selin</creatorcontrib><creatorcontrib>Saldmann, Frédéric</creatorcontrib><creatorcontrib>Viltard, Melanie</creatorcontrib><creatorcontrib>Friedlander, Gérard</creatorcontrib><creatorcontrib>Faulkes, Chris G</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Aging (Albany, NY.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lowe, Robert</au><au>Danson, Amy F</au><au>Rakyan, Vardhman K</au><au>Yildizoglu, Selin</au><au>Saldmann, Frédéric</au><au>Viltard, Melanie</au><au>Friedlander, Gérard</au><au>Faulkes, Chris G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA methylation clocks as a predictor for ageing and age estimation in naked mole-rats, Heterocephalus glaber</atitle><jtitle>Aging (Albany, NY.)</jtitle><addtitle>Aging (Albany NY)</addtitle><date>2020-03-03</date><risdate>2020</risdate><volume>12</volume><issue>5</issue><spage>4394</spage><epage>4406</epage><pages>4394-4406</pages><issn>1945-4589</issn><eissn>1945-4589</eissn><abstract>The naked mole-rat,
(NMR), the longest-lived rodent, is of significance and interest in the study of biomarkers for ageing. Recent breakthroughs in this field have revealed 'epigenetic clocks' that are based on the temporal accumulation of DNA methylation at specific genomic sites. Here, we validate the hypothesis of an epigenetic clock in NMRs based on changes in methylation of targeted CpG sites. We initially analysed 51 CpGs in NMR livers spanning an age range of 39-1,144 weeks and found 23 to be significantly associated with age (p<0.05). We then built a predictor of age using these sites. To test the accuracy of this model, we analysed an additional set of liver samples, and were successfully able to predict their age with a root mean squared error of 166 weeks. We also profiled skin samples with the same age range, finding a striking correlation between their predicted age versus their actual age (R=0.93), but which was lower when compared to the liver, suggesting that skin ages slower than the liver in NMRs. Our model will enable the prediction of age in wild-caught and captive NMRs of unknown age, and will be invaluable for further mechanistic studies of mammalian ageing.</abstract><cop>United States</cop><pub>Impact Journals</pub><pmid>32126024</pmid><doi>10.18632/aging.102892</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Aging - genetics Aging - metabolism Animals CpG Islands - genetics DNA Methylation Liver - metabolism Mole Rats Research Paper Skin - metabolism |
| title | DNA methylation clocks as a predictor for ageing and age estimation in naked mole-rats, Heterocephalus glaber |
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