Induction of Th1 type response by DNA vaccinations with N, M, and E genes against SARS-CoV in mice
Vaccination against the SARS-CoV infection is an attractive means to control the spread of viruses in public. In this study, we employed a DNA vaccine technology with the levamisole, our newly discovered chemical adjuvant, to generate Th1 type of response. To avoid the enhancement antibody issue, ge...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2005-03, Vol.328 (4), p.979-986 |
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| creator | Jin, Huali Xiao, Chong Chen, Ze Kang, Youmin Ma, Yijie Zhu, Kaichun Xie, Qifa Tu, Yixian Yu, Yang Wang, Bin |
| description | Vaccination against the SARS-CoV infection is an attractive means to control the spread of viruses in public. In this study, we employed a DNA vaccine technology with the levamisole, our newly discovered chemical adjuvant, to generate Th1 type of response. To avoid the enhancement antibody issue, genes encoding the nucleocapsid, membrane, and envelope protein of SARS-CoV were cloned and their expressions in mammalian cells were determined. After the intramuscular introduction into animals, we observed that the constructs of the E, M, and N genes could induce high levels of specific antibodies, T cell proliferations, IFN-γ, DTH responses, and in vivo cytotoxic T cells activities specifically against SARS-CoV antigens. The highest immune responses were generated by the construct encoding the nucleocapsid protein. The results suggest that the N, M, and E genes could be used as the targets to prevent SARS-CoV infection in the DNA vaccine development. |
| doi_str_mv | 10.1016/j.bbrc.2005.01.048 |
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In this study, we employed a DNA vaccine technology with the levamisole, our newly discovered chemical adjuvant, to generate Th1 type of response. To avoid the enhancement antibody issue, genes encoding the nucleocapsid, membrane, and envelope protein of SARS-CoV were cloned and their expressions in mammalian cells were determined. After the intramuscular introduction into animals, we observed that the constructs of the E, M, and N genes could induce high levels of specific antibodies, T cell proliferations, IFN-γ, DTH responses, and in vivo cytotoxic T cells activities specifically against SARS-CoV antigens. The highest immune responses were generated by the construct encoding the nucleocapsid protein. The results suggest that the N, M, and E genes could be used as the targets to prevent SARS-CoV infection in the DNA vaccine development.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2005.01.048</identifier><identifier>PMID: 15707974</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adjuvants, Immunologic - metabolism ; Animals ; Chemotherapy, Adjuvant - methods ; Cytokines - immunology ; DNA vaccine ; Envelop protein ; Female ; Genetic Therapy - methods ; In vivo CTL ; Levamisole - administration & dosage ; Lymphocyte Activation - drug effects ; Lymphocyte Activation - genetics ; Lymphocyte Activation - immunology ; Membrane protein ; Mice ; Mice, Inbred BALB C ; Nucleocapsid protein ; Nucleocapsid Proteins - genetics ; Nucleocapsid Proteins - immunology ; SARS coronavirus ; SARS-CoV ; Severe Acute Respiratory Syndrome - genetics ; Severe Acute Respiratory Syndrome - immunology ; Severe Acute Respiratory Syndrome - prevention & control ; Th1 Cells - drug effects ; Th1 Cells - immunology ; Th1 response ; Vaccines, DNA - administration & dosage ; Vaccines, DNA - genetics</subject><ispartof>Biochemical and biophysical research communications, 2005-03, Vol.328 (4), p.979-986</ispartof><rights>2005 Elsevier Inc.</rights><rights>Copyright © 2005 Elsevier Inc. All rights reserved. 2005 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c484t-198d39bfc998118af60ddba1e95f1e8b4d0a6db3725d6cd3de2d7339d4e5637d3</citedby><cites>FETCH-LOGICAL-c484t-198d39bfc998118af60ddba1e95f1e8b4d0a6db3725d6cd3de2d7339d4e5637d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X05001026$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15707974$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jin, Huali</creatorcontrib><creatorcontrib>Xiao, Chong</creatorcontrib><creatorcontrib>Chen, Ze</creatorcontrib><creatorcontrib>Kang, Youmin</creatorcontrib><creatorcontrib>Ma, Yijie</creatorcontrib><creatorcontrib>Zhu, Kaichun</creatorcontrib><creatorcontrib>Xie, Qifa</creatorcontrib><creatorcontrib>Tu, Yixian</creatorcontrib><creatorcontrib>Yu, Yang</creatorcontrib><creatorcontrib>Wang, Bin</creatorcontrib><title>Induction of Th1 type response by DNA vaccinations with N, M, and E genes against SARS-CoV in mice</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Vaccination against the SARS-CoV infection is an attractive means to control the spread of viruses in public. In this study, we employed a DNA vaccine technology with the levamisole, our newly discovered chemical adjuvant, to generate Th1 type of response. To avoid the enhancement antibody issue, genes encoding the nucleocapsid, membrane, and envelope protein of SARS-CoV were cloned and their expressions in mammalian cells were determined. After the intramuscular introduction into animals, we observed that the constructs of the E, M, and N genes could induce high levels of specific antibodies, T cell proliferations, IFN-γ, DTH responses, and in vivo cytotoxic T cells activities specifically against SARS-CoV antigens. The highest immune responses were generated by the construct encoding the nucleocapsid protein. The results suggest that the N, M, and E genes could be used as the targets to prevent SARS-CoV infection in the DNA vaccine development.</description><subject>Adjuvants, Immunologic - metabolism</subject><subject>Animals</subject><subject>Chemotherapy, Adjuvant - methods</subject><subject>Cytokines - immunology</subject><subject>DNA vaccine</subject><subject>Envelop protein</subject><subject>Female</subject><subject>Genetic Therapy - methods</subject><subject>In vivo CTL</subject><subject>Levamisole - administration & dosage</subject><subject>Lymphocyte Activation - drug effects</subject><subject>Lymphocyte Activation - genetics</subject><subject>Lymphocyte Activation - immunology</subject><subject>Membrane protein</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Nucleocapsid protein</subject><subject>Nucleocapsid Proteins - genetics</subject><subject>Nucleocapsid Proteins - immunology</subject><subject>SARS coronavirus</subject><subject>SARS-CoV</subject><subject>Severe Acute Respiratory Syndrome - genetics</subject><subject>Severe Acute Respiratory Syndrome - immunology</subject><subject>Severe Acute Respiratory Syndrome - prevention & control</subject><subject>Th1 Cells - drug effects</subject><subject>Th1 Cells - immunology</subject><subject>Th1 response</subject><subject>Vaccines, DNA - administration & dosage</subject><subject>Vaccines, DNA - genetics</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2P0zAQhi0EYsvCH-CAfOK0CTOJE8cSQqrKLqy0LBK7IG6WY09aV61T7LSo_35TteLjAqc5zDOvZuZh7CVCjoD1m2XettHmBUCVA-YgmkdsgqAgKxDEYzYBgDorFH4_Y89SWgIgilo9ZWdYSZBKiglrr4Pb2sH3gfcdv18gH_Yb4pHSpg-JeLvn72-nfGes9cEcuMR_-mHBby_4pwtuguOXfE6BEjdz40Ma-N30y102679xH_jaW3rOnnRmlejFqZ6zr1eX97OP2c3nD9ez6U1mRSOGDFXjStV2VqkGsTFdDc61BklVHVLTCgemdm0pi8rV1pWOCifLUjlBVV1KV56zd8fczbZdk7MUhmhWehP92sS97o3Xf3eCX-h5v9MSVNGIegx4fQqI_Y8tpUGvfbK0WplA_TbpWopCiqr4L4iyKVGIcgSLI2hjn1Kk7tc2CPrgUC_1waE-ONSAenQ4Dr36847fIydpI_D2CND4zZ2nqJP1FCw5H8kO2vX-X_kP6YatBQ</recordid><startdate>20050325</startdate><enddate>20050325</enddate><creator>Jin, Huali</creator><creator>Xiao, Chong</creator><creator>Chen, Ze</creator><creator>Kang, Youmin</creator><creator>Ma, Yijie</creator><creator>Zhu, Kaichun</creator><creator>Xie, Qifa</creator><creator>Tu, Yixian</creator><creator>Yu, Yang</creator><creator>Wang, Bin</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20050325</creationdate><title>Induction of Th1 type response by DNA vaccinations with N, M, and E genes against SARS-CoV in mice</title><author>Jin, Huali ; 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| subjects | Adjuvants, Immunologic - metabolism Animals Chemotherapy, Adjuvant - methods Cytokines - immunology DNA vaccine Envelop protein Female Genetic Therapy - methods In vivo CTL Levamisole - administration & dosage Lymphocyte Activation - drug effects Lymphocyte Activation - genetics Lymphocyte Activation - immunology Membrane protein Mice Mice, Inbred BALB C Nucleocapsid protein Nucleocapsid Proteins - genetics Nucleocapsid Proteins - immunology SARS coronavirus SARS-CoV Severe Acute Respiratory Syndrome - genetics Severe Acute Respiratory Syndrome - immunology Severe Acute Respiratory Syndrome - prevention & control Th1 Cells - drug effects Th1 Cells - immunology Th1 response Vaccines, DNA - administration & dosage Vaccines, DNA - genetics |
| title | Induction of Th1 type response by DNA vaccinations with N, M, and E genes against SARS-CoV in mice |
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