Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii
Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.Therefore, bats may have evolved effective mechanisms to control viral replication. However, little information is available on bat immune responses to viral infection. Type I interferon(IFN) plays a key role...
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| Veröffentlicht in: | Virologica Sinica 2015-12, Vol.30 (6), p.425-432 |
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| creator | Liang, Ying-Zi Wu, Li-Jun Zhang, Qian Zhou, Peng Wang, Mei-Niang Yang, Xing-Lou Ge, Xing-Yi Wang, Lin-Fa Shi, Zheng-Li |
| description | Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.Therefore, bats may have evolved effective mechanisms to control viral replication. However, little information is available on bat immune responses to viral infection. Type I interferon(IFN) plays a key role in controlling viral infections. In this study, we report the cloning, expression, and biological activity of interferon β(IFNβ) from the Chinese microbat species, Myotis davidii. We demonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from M. davidii after treatment with poly I:C or infection with Sendai virus. Furthermore, the recombinant IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat species, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral activity in microbats, which has important implications for virus interactions with these hosts. |
| doi_str_mv | 10.1007/s12250-015-3668-2 |
| format | Article |
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However, little information is available on bat immune responses to viral infection. Type I interferon(IFN) plays a key role in controlling viral infections. In this study, we report the cloning, expression, and biological activity of interferon β(IFNβ) from the Chinese microbat species, Myotis davidii. We demonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from M. davidii after treatment with poly I:C or infection with Sendai virus. Furthermore, the recombinant IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat species, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral activity in microbats, which has important implications for virus interactions with these hosts.</description><identifier>ISSN: 1674-0769</identifier><identifier>EISSN: 1995-820X</identifier><identifier>DOI: 10.1007/s12250-015-3668-2</identifier><identifier>PMID: 26645237</identifier><language>eng</language><publisher>Wuhan: Wuhan Institute of Virology, CAS</publisher><subject>activity ; Adenovirus ; Amino Acid Sequence ; Animals ; Antiviral activity ; Antiviral Agents - pharmacology ; Antiviral drugs ; antiviral properties ; bat;interferon;IFN-stimulated ; Bats ; Biochemistry ; Biological activity ; Biomedical and Life Sciences ; Biomedicine ; Cell Line ; Cell lines ; Chiroptera ; Chiroptera - genetics ; Chiroptera - immunology ; Chiroptera - virology ; Cloning, Molecular ; disease reservoirs ; gene expression regulation ; genes ; genes;antiviral ; hosts ; Humans ; Immune response ; Immunity, Innate ; Interferon Type I - pharmacology ; Interferon-beta - biosynthesis ; Interferon-beta - genetics ; Interferon-beta - immunology ; Interferon-beta - pharmacology ; interferons ; kidneys ; Medical Microbiology ; Microbial Genetics and Genomics ; Microbiology ; Molecular Sequence Data ; Murine respirovirus ; Myotis ; Myotis davidii ; Oncology ; Phylogeny ; Polyinosinic:polycytidylic acid ; Replication ; Research Article ; Rhinolophus ; Rhinolophus sinicus ; Sendai virus ; Sequence Alignment ; Sequence Analysis, Protein ; Sequence Homology ; Stomatitis ; Up-Regulation ; Vesicular stomatitis virus ; Vesiculovirus ; Vesiculovirus - drug effects ; Vesiculovirus - physiology ; Viral infections ; Virology ; virus replication ; Viruses ; β-Interferon</subject><ispartof>Virologica Sinica, 2015-12, Vol.30 (6), p.425-432</ispartof><rights>Wuhan Institute of Virology, CAS and Springer Science+Business Media Singapore Pte Ltd 2015</rights><rights>2015© Wuhan Institute of Virology, CAS and Springer Science+Business Media Singapore Pte Ltd 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c650t-e27e336a29df77b528fa24f7bc5c0c1bd04ea3d7e08512653e3d53fe95fee9993</citedby><cites>FETCH-LOGICAL-c650t-e27e336a29df77b528fa24f7bc5c0c1bd04ea3d7e08512653e3d53fe95fee9993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/92590B/92590B.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7091266/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7091266/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,41464,42533,51294,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26645237$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liang, Ying-Zi</creatorcontrib><creatorcontrib>Wu, Li-Jun</creatorcontrib><creatorcontrib>Zhang, Qian</creatorcontrib><creatorcontrib>Zhou, Peng</creatorcontrib><creatorcontrib>Wang, Mei-Niang</creatorcontrib><creatorcontrib>Yang, Xing-Lou</creatorcontrib><creatorcontrib>Ge, Xing-Yi</creatorcontrib><creatorcontrib>Wang, Lin-Fa</creatorcontrib><creatorcontrib>Shi, Zheng-Li</creatorcontrib><title>Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii</title><title>Virologica Sinica</title><addtitle>Virol. Sin</addtitle><addtitle>Virologica Sinica</addtitle><description>Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.Therefore, bats may have evolved effective mechanisms to control viral replication. However, little information is available on bat immune responses to viral infection. Type I interferon(IFN) plays a key role in controlling viral infections. In this study, we report the cloning, expression, and biological activity of interferon β(IFNβ) from the Chinese microbat species, Myotis davidii. We demonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from M. davidii after treatment with poly I:C or infection with Sendai virus. Furthermore, the recombinant IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat species, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral activity in microbats, which has important implications for virus interactions with these hosts.</description><subject>activity</subject><subject>Adenovirus</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antiviral activity</subject><subject>Antiviral Agents - pharmacology</subject><subject>Antiviral drugs</subject><subject>antiviral properties</subject><subject>bat;interferon;IFN-stimulated</subject><subject>Bats</subject><subject>Biochemistry</subject><subject>Biological activity</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Chiroptera</subject><subject>Chiroptera - genetics</subject><subject>Chiroptera - immunology</subject><subject>Chiroptera - virology</subject><subject>Cloning, Molecular</subject><subject>disease reservoirs</subject><subject>gene expression regulation</subject><subject>genes</subject><subject>genes;antiviral</subject><subject>hosts</subject><subject>Humans</subject><subject>Immune response</subject><subject>Immunity, Innate</subject><subject>Interferon Type I - pharmacology</subject><subject>Interferon-beta - biosynthesis</subject><subject>Interferon-beta - genetics</subject><subject>Interferon-beta - immunology</subject><subject>Interferon-beta - pharmacology</subject><subject>interferons</subject><subject>kidneys</subject><subject>Medical Microbiology</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Murine respirovirus</subject><subject>Myotis</subject><subject>Myotis davidii</subject><subject>Oncology</subject><subject>Phylogeny</subject><subject>Polyinosinic:polycytidylic acid</subject><subject>Replication</subject><subject>Research Article</subject><subject>Rhinolophus</subject><subject>Rhinolophus sinicus</subject><subject>Sendai virus</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, Protein</subject><subject>Sequence Homology</subject><subject>Stomatitis</subject><subject>Up-Regulation</subject><subject>Vesicular stomatitis virus</subject><subject>Vesiculovirus</subject><subject>Vesiculovirus - drug effects</subject><subject>Vesiculovirus - physiology</subject><subject>Viral infections</subject><subject>Virology</subject><subject>virus replication</subject><subject>Viruses</subject><subject>β-Interferon</subject><issn>1674-0769</issn><issn>1995-820X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcmOEzEQhlsIxAwDD8AFWeLCIQ3elwsSitikQVxA4ma5u8uJR912xu5E5LV4EJ4JRwlhOcDBckn11V_L3zSPCX5OMFYvCqFU4BYT0TIpdUvvNJfEGNFqir_crbFUvMVKmovmQSk3GEuqGbvfXFApuaBMXTZ-OaYY4mqB4OsmQykhxQVycahvDruQ3Yhcf4jmPUoehThD9pBTRN-_IZ_ThOY1oOU6RCiAptDn1Ll5gT7s0xwKGtwuDCE8bO55NxZ4dPqvms9vXn9avmuvP759v3x13fZS4LkFqoAx6agZvFKdoNo7yr3qetHjnnQD5uDYoABrQagUDNggmAcjPIAxhl01L4-6m203wdBDnOsGdpPD5PLeJhfsn5kY1naVdlZhUwVlFXh2EsjpdgtltlMoPYyji5C2xRKNGeFGav5_VGllFJeYVPTpX-hN2uZYL2GrCxobrQ2tFDlS9YalZPDnuQm2B8Pt0XBbDbcHw-2h5snvC58rfjpcAXoESk3FFeRfrf-lyk6TrFNc3da6s7DBsjJaC8w1N4JxLXiNtGDsByEkxwc</recordid><startdate>20151201</startdate><enddate>20151201</enddate><creator>Liang, Ying-Zi</creator><creator>Wu, Li-Jun</creator><creator>Zhang, Qian</creator><creator>Zhou, Peng</creator><creator>Wang, Mei-Niang</creator><creator>Yang, Xing-Lou</creator><creator>Ge, Xing-Yi</creator><creator>Wang, Lin-Fa</creator><creator>Shi, Zheng-Li</creator><general>Wuhan Institute of Virology, CAS</general><general>KeAi Publishing Communications Ltd</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7U9</scope><scope>H94</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope></search><sort><creationdate>20151201</creationdate><title>Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii</title><author>Liang, Ying-Zi ; 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Sin</stitle><addtitle>Virologica Sinica</addtitle><date>2015-12-01</date><risdate>2015</risdate><volume>30</volume><issue>6</issue><spage>425</spage><epage>432</epage><pages>425-432</pages><issn>1674-0769</issn><eissn>1995-820X</eissn><abstract>Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.Therefore, bats may have evolved effective mechanisms to control viral replication. However, little information is available on bat immune responses to viral infection. Type I interferon(IFN) plays a key role in controlling viral infections. In this study, we report the cloning, expression, and biological activity of interferon β(IFNβ) from the Chinese microbat species, Myotis davidii. We demonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from M. davidii after treatment with poly I:C or infection with Sendai virus. Furthermore, the recombinant IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat species, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral activity in microbats, which has important implications for virus interactions with these hosts.</abstract><cop>Wuhan</cop><pub>Wuhan Institute of Virology, CAS</pub><pmid>26645237</pmid><doi>10.1007/s12250-015-3668-2</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Virologica Sinica, 2015-12, Vol.30 (6), p.425-432 |
| issn | 1674-0769 1995-820X |
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| subjects | activity Adenovirus Amino Acid Sequence Animals Antiviral activity Antiviral Agents - pharmacology Antiviral drugs antiviral properties bat interferon IFN-stimulated Bats Biochemistry Biological activity Biomedical and Life Sciences Biomedicine Cell Line Cell lines Chiroptera Chiroptera - genetics Chiroptera - immunology Chiroptera - virology Cloning, Molecular disease reservoirs gene expression regulation genes genes antiviral hosts Humans Immune response Immunity, Innate Interferon Type I - pharmacology Interferon-beta - biosynthesis Interferon-beta - genetics Interferon-beta - immunology Interferon-beta - pharmacology interferons kidneys Medical Microbiology Microbial Genetics and Genomics Microbiology Molecular Sequence Data Murine respirovirus Myotis Myotis davidii Oncology Phylogeny Polyinosinic:polycytidylic acid Replication Research Article Rhinolophus Rhinolophus sinicus Sendai virus Sequence Alignment Sequence Analysis, Protein Sequence Homology Stomatitis Up-Regulation Vesicular stomatitis virus Vesiculovirus Vesiculovirus - drug effects Vesiculovirus - physiology Viral infections Virology virus replication Viruses β-Interferon |
| title | Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii |
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