Respiratory Virus Multiplex RT-PCR Assay Sensitivities and Influence Factors in Hospitalized Children with Lower Respiratory Tract Infections

Multiplex RT-PCR assays have been widely used tools for detection and differentiation of a panel of respiratory viral pathogens. In this study, we evaluated the Qiagen ResPlex lI V2.0 kit and explored factors influencing its sensitivity. Nasopharyngeal swab （NPS） specimens were prospectively collect...

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Veröffentlicht in:	Virologica Sinica 2013-04, Vol.28 (2), p.97-102
Hauptverfasser:	Deng, Jikui, Ma, Zhuoya, Huang, Wenbo, Li, Chengrong, Wang, Heping, Zheng, Yuejie, Zhou, Rong, Tang, Yi-Wei
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenoviridae A型流感病毒 Biochemistry Biomedical and Life Sciences Biomedicine Cell culture Child Child, Hospitalized Child, Preschool Children Coronaviridae Coronaviruses Enterovirus Enterovirus - pathogenicity Female genotype Genotypes Human metapneumovirus Humans Infant Infant, Newborn Influenza Influenza A Influenza A virus Influenza A virus - pathogenicity Male Medical Microbiology Microbial Genetics and Genomics Microbiology Multiplex Polymerase Chain Reaction - methods nucleic acids Oncology Pandemics Parainfluenza Pathogens patients Pediatrics Polymerase chain reaction Research Article Respiratory syncytial virus respiratory system Respiratory tract diseases Respiratory tract infection Respiratory Tract Infections - diagnosis Respiratory Tract Infections - genetics Respiratory Tract Infections - virology Reverse Transcriptase Polymerase Chain Reaction - methods Rhinovirus Rhinovirus - pathogenicity RT-PCR检测 Sensitivity and Specificity viral load Virology Virus Diseases - diagnosis Virus Diseases - genetics Virus Diseases - virology Viruses 儿童医院呼吸道感染多重PCR技术影响因素敏感性荧光定量RT-PCR
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