Systematic alteration of ATAC-seq for profiling open chromatin in cryopreserved nuclei preparations from livestock tissues

The use of Assay for Transposase-Accessible Chromatin (ATAC-seq) to profile chromatin accessibility has surged over the past years, but its applicability to tissues has been very limited. With the intent of preserving nuclear architecture during long-term storage, cryopreserved nuclei preparations f...

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Veröffentlicht in:	Scientific reports 2020-03, Vol.10 (1), p.5230-5230, Article 5230
Hauptverfasser:	Halstead, M. M., Kern, C., Saelao, P., Chanthavixay, G., Wang, Y., Delany, M. E., Zhou, H., Ross, P. J.
Format:	Artikel
Sprache:	eng
Schlagworte:	13 38/23 45/15 631/114/2400 631/1647/2210/2211 Animal tissues Animals Cell Nucleus - genetics Chickens Chromatin Chromatin - metabolism Chromatin Immunoprecipitation Sequencing - methods Cryopreservation Cryopreservation - methods Deoxyribonuclease Deoxyribonuclease I - metabolism DNA, Intergenic Gene expression Genomes High-Throughput Nucleotide Sequencing - methods Humanities and Social Sciences Livestock Lung - cytology Male multidisciplinary Muscle, Skeletal - cytology Nuclei Promoter Regions, Genetic Ribonucleic acid RNA Science Science (multidisciplinary) Spleen - cytology Sucrose Swine Transcription factors Transposase
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