Inhibition of avian leukosis virus subgroup J replication by miRNA targeted against env
No effective vaccine has been developed against the subgroup J avian leukosis virus (ALV-J). The genetic diversity of ALV-J might be related to the env gene, therefore, we selected conserved sequences of the env gene and designed interference sequence. In this study, microRNAs (miRNAs) were designed...
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| Veröffentlicht in: | Virus genes 2013-08, Vol.47 (1), p.34-41 |
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| creator | Wang, Wei Zhang, Zai-Ping Tian, Jin Xiao, Zhi-Guang Meng, Qing-Wen |
| description | No effective vaccine has been developed against the subgroup J avian leukosis virus (ALV-J). The genetic diversity of ALV-J might be related to the env gene, therefore, we selected conserved sequences of the env gene and designed interference sequence. In this study, microRNAs (miRNAs) were designed and synthesized, corresponding to conserved regions of the env gene. These miRNAs were cloned into the linearized eukaryotic expression vector. The recombinant plasmids were transfected into DF-1 cells. After transfection, the cells were inoculated with ALV-J. In reporter assays, the transfection efficiency is 80 % by indirect immunofluorescence (IFA). Expression of the virus envelope glycoprotein was measured by IFA and western blotting assays. The relative expression of env gene was determined using quantitative PCR. Our results show that the mi-env 231 and mi-env 1384 could effectively suppress the replication of ALV-J with an efficiency of 68.7–75.2 %. These data suggest that the miRNAs targeting the env can inhibit replication of ALV-J efficiently. This finding provides evidence that miRNAs could be used as a potential tool against ALV infection. |
| doi_str_mv | 10.1007/s11262-013-0906-2 |
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The genetic diversity of ALV-J might be related to the env gene, therefore, we selected conserved sequences of the env gene and designed interference sequence. In this study, microRNAs (miRNAs) were designed and synthesized, corresponding to conserved regions of the env gene. These miRNAs were cloned into the linearized eukaryotic expression vector. The recombinant plasmids were transfected into DF-1 cells. After transfection, the cells were inoculated with ALV-J. In reporter assays, the transfection efficiency is 80 % by indirect immunofluorescence (IFA). Expression of the virus envelope glycoprotein was measured by IFA and western blotting assays. The relative expression of env gene was determined using quantitative PCR. Our results show that the mi-env 231 and mi-env 1384 could effectively suppress the replication of ALV-J with an efficiency of 68.7–75.2 %. These data suggest that the miRNAs targeting the env can inhibit replication of ALV-J efficiently. This finding provides evidence that miRNAs could be used as a potential tool against ALV infection.</description><identifier>ISSN: 0920-8569</identifier><identifier>EISSN: 1572-994X</identifier><identifier>DOI: 10.1007/s11262-013-0906-2</identifier><identifier>PMID: 23546824</identifier><language>eng</language><publisher>Boston: Springer-Verlag</publisher><subject>Animals ; Avian Leukosis - therapy ; Avian Leukosis - virology ; Avian leukosis virus ; Avian Leukosis Virus - genetics ; Avian Leukosis Virus - physiology ; Biomedical and Life Sciences ; Biomedicine ; Cell Line ; Chickens ; conserved sequences ; fluorescent antibody technique ; genes ; Genetic Therapy - veterinary ; genetic variation ; glycoproteins ; Medical Microbiology ; microRNA ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Plant Sciences ; plasmids ; polymerase chain reaction ; Poultry Diseases - therapy ; Poultry Diseases - virology ; RNA, Viral - genetics ; transfection ; Viral Envelope Proteins - genetics ; Viral Envelope Proteins - metabolism ; Virology ; Virus Replication ; viruses ; Western blotting</subject><ispartof>Virus genes, 2013-08, Vol.47 (1), p.34-41</ispartof><rights>Springer Science+Business Media New York 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c527t-31ca653ae55a18fbcdc921518cebf89ddb9d71cbfde214a63d17e9b4d3d20b923</citedby><cites>FETCH-LOGICAL-c527t-31ca653ae55a18fbcdc921518cebf89ddb9d71cbfde214a63d17e9b4d3d20b923</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11262-013-0906-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11262-013-0906-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,780,784,885,27915,27916,41479,42548,51310</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23546824$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Wei</creatorcontrib><creatorcontrib>Zhang, Zai-Ping</creatorcontrib><creatorcontrib>Tian, Jin</creatorcontrib><creatorcontrib>Xiao, Zhi-Guang</creatorcontrib><creatorcontrib>Meng, Qing-Wen</creatorcontrib><title>Inhibition of avian leukosis virus subgroup J replication by miRNA targeted against env</title><title>Virus genes</title><addtitle>Virus Genes</addtitle><addtitle>Virus Genes</addtitle><description>No effective vaccine has been developed against the subgroup J avian leukosis virus (ALV-J). The genetic diversity of ALV-J might be related to the env gene, therefore, we selected conserved sequences of the env gene and designed interference sequence. In this study, microRNAs (miRNAs) were designed and synthesized, corresponding to conserved regions of the env gene. These miRNAs were cloned into the linearized eukaryotic expression vector. The recombinant plasmids were transfected into DF-1 cells. After transfection, the cells were inoculated with ALV-J. In reporter assays, the transfection efficiency is 80 % by indirect immunofluorescence (IFA). Expression of the virus envelope glycoprotein was measured by IFA and western blotting assays. The relative expression of env gene was determined using quantitative PCR. Our results show that the mi-env 231 and mi-env 1384 could effectively suppress the replication of ALV-J with an efficiency of 68.7–75.2 %. These data suggest that the miRNAs targeting the env can inhibit replication of ALV-J efficiently. This finding provides evidence that miRNAs could be used as a potential tool against ALV infection.</description><subject>Animals</subject><subject>Avian Leukosis - therapy</subject><subject>Avian Leukosis - virology</subject><subject>Avian leukosis virus</subject><subject>Avian Leukosis Virus - genetics</subject><subject>Avian Leukosis Virus - physiology</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Line</subject><subject>Chickens</subject><subject>conserved sequences</subject><subject>fluorescent antibody technique</subject><subject>genes</subject><subject>Genetic Therapy - veterinary</subject><subject>genetic variation</subject><subject>glycoproteins</subject><subject>Medical Microbiology</subject><subject>microRNA</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>Plant Sciences</subject><subject>plasmids</subject><subject>polymerase chain reaction</subject><subject>Poultry Diseases - therapy</subject><subject>Poultry Diseases - virology</subject><subject>RNA, Viral - genetics</subject><subject>transfection</subject><subject>Viral Envelope Proteins - genetics</subject><subject>Viral Envelope Proteins - metabolism</subject><subject>Virology</subject><subject>Virus Replication</subject><subject>viruses</subject><subject>Western blotting</subject><issn>0920-8569</issn><issn>1572-994X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkk1vFSEUhonR2NvqD3CjJG66GT0HZhjYmDSNHzWNJmqjOwIDM6XOhSvM3KT_3rne2lQXxhUk5zkvHB4IeYLwAgHalwWRCVYB8goUiIrdIytsWlYpVX-7T1agGFSyEeqAHJZyBQBSsvohOWC8qcWyXZGvZ_Ey2DCFFGnqqdkGE-no5--phEK3Ic-FltkOOc0b-p5mvxlDZ37h9pquw6cPJ3QyefCTd9QMJsQyUR-3j8iD3ozFP75Zj8jFm9dfTt9V5x_fnp2enFddw9qp4tgZ0XDjm8ag7G3nOsWwQdl520vlnFWuxc72zjOsjeAOW69s7bhjYBXjR-TVPncz27V3nY9TNqPe5LA2-VonE_SflRgu9ZC2ugWpkOMScHwTkNOP2ZdJr0Pp_Dia6NNcNNasBeBCwn-gKLngNcgFff4XepXmHJeX2FGtEghCLBTuqS6nUrLvb--NoHeG9d6wXgzrnWG9G_jp3YFvO34rXQC2B8pSioPPd47-R-qzfVNvkjZDDkVffGaA9fJnGONM8p9KVLtN</recordid><startdate>20130801</startdate><enddate>20130801</enddate><creator>Wang, Wei</creator><creator>Zhang, Zai-Ping</creator><creator>Tian, Jin</creator><creator>Xiao, Zhi-Guang</creator><creator>Meng, Qing-Wen</creator><general>Springer-Verlag</general><general>Springer US</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20130801</creationdate><title>Inhibition of avian leukosis virus subgroup J replication by miRNA targeted against env</title><author>Wang, Wei ; Zhang, Zai-Ping ; Tian, Jin ; Xiao, Zhi-Guang ; Meng, Qing-Wen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c527t-31ca653ae55a18fbcdc921518cebf89ddb9d71cbfde214a63d17e9b4d3d20b923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Avian Leukosis - therapy</topic><topic>Avian Leukosis - virology</topic><topic>Avian leukosis virus</topic><topic>Avian Leukosis Virus - genetics</topic><topic>Avian Leukosis Virus - physiology</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell Line</topic><topic>Chickens</topic><topic>conserved sequences</topic><topic>fluorescent antibody technique</topic><topic>genes</topic><topic>Genetic Therapy - veterinary</topic><topic>genetic variation</topic><topic>glycoproteins</topic><topic>Medical Microbiology</topic><topic>microRNA</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>Plant Sciences</topic><topic>plasmids</topic><topic>polymerase chain reaction</topic><topic>Poultry Diseases - therapy</topic><topic>Poultry Diseases - virology</topic><topic>RNA, Viral - genetics</topic><topic>transfection</topic><topic>Viral Envelope Proteins - genetics</topic><topic>Viral Envelope Proteins - metabolism</topic><topic>Virology</topic><topic>Virus Replication</topic><topic>viruses</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Wei</creatorcontrib><creatorcontrib>Zhang, Zai-Ping</creatorcontrib><creatorcontrib>Tian, Jin</creatorcontrib><creatorcontrib>Xiao, Zhi-Guang</creatorcontrib><creatorcontrib>Meng, Qing-Wen</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health Medical collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Virus genes</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Wei</au><au>Zhang, Zai-Ping</au><au>Tian, Jin</au><au>Xiao, Zhi-Guang</au><au>Meng, Qing-Wen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of avian leukosis virus subgroup J replication by miRNA targeted against env</atitle><jtitle>Virus genes</jtitle><stitle>Virus Genes</stitle><addtitle>Virus Genes</addtitle><date>2013-08-01</date><risdate>2013</risdate><volume>47</volume><issue>1</issue><spage>34</spage><epage>41</epage><pages>34-41</pages><issn>0920-8569</issn><eissn>1572-994X</eissn><abstract>No effective vaccine has been developed against the subgroup J avian leukosis virus (ALV-J). The genetic diversity of ALV-J might be related to the env gene, therefore, we selected conserved sequences of the env gene and designed interference sequence. In this study, microRNAs (miRNAs) were designed and synthesized, corresponding to conserved regions of the env gene. These miRNAs were cloned into the linearized eukaryotic expression vector. The recombinant plasmids were transfected into DF-1 cells. After transfection, the cells were inoculated with ALV-J. In reporter assays, the transfection efficiency is 80 % by indirect immunofluorescence (IFA). Expression of the virus envelope glycoprotein was measured by IFA and western blotting assays. The relative expression of env gene was determined using quantitative PCR. Our results show that the mi-env 231 and mi-env 1384 could effectively suppress the replication of ALV-J with an efficiency of 68.7–75.2 %. These data suggest that the miRNAs targeting the env can inhibit replication of ALV-J efficiently. This finding provides evidence that miRNAs could be used as a potential tool against ALV infection.</abstract><cop>Boston</cop><pub>Springer-Verlag</pub><pmid>23546824</pmid><doi>10.1007/s11262-013-0906-2</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Avian Leukosis - therapy Avian Leukosis - virology Avian leukosis virus Avian Leukosis Virus - genetics Avian Leukosis Virus - physiology Biomedical and Life Sciences Biomedicine Cell Line Chickens conserved sequences fluorescent antibody technique genes Genetic Therapy - veterinary genetic variation glycoproteins Medical Microbiology microRNA MicroRNAs - genetics MicroRNAs - metabolism Plant Sciences plasmids polymerase chain reaction Poultry Diseases - therapy Poultry Diseases - virology RNA, Viral - genetics transfection Viral Envelope Proteins - genetics Viral Envelope Proteins - metabolism Virology Virus Replication viruses Western blotting |
| title | Inhibition of avian leukosis virus subgroup J replication by miRNA targeted against env |
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