A highly conserved epitope on the spike protein of infectious bronchitis virus
The predicted amino acid sequence and secondary structures of S1 of the spike protein (S) of infectious bronchitis viral (IBV) strains from Europe, the U.S.A., and Japan were compared. An antigenic determinant that was highly conserved in both the primary amino acid sequence and secondary structure...
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| Veröffentlicht in: | Archives of virology 1995-01, Vol.140 (12), p.2201-2213 |
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| creator | WANG, L PARR, R. L KING, D. J COLLISSON, E. W |
| description | The predicted amino acid sequence and secondary structures of S1 of the spike protein (S) of infectious bronchitis viral (IBV) strains from Europe, the U.S.A., and Japan were compared. An antigenic determinant that was highly conserved in both the primary amino acid sequence and secondary structure of all strains was identified between amino acid positions 240 to 255. A synthesized peptide corresponding to this region was found to react with all polyclonal antisera examined from various IBV strains and with one monoclonal antibody (MAb), 9B1B6, out of nine known to react with the S of Gray. The specificity of the interaction with MAb 9B1B6 was confirmed by competitive ELISA using bound and unbound peptide. Interestingly, the previously described epitope for 9B1B6 had been characterized as cross-reactive with several strains of IBV, as conformation-independent but reacting only with intact whole S, and as associated with the functional integrity of other epitopes, including neutralizing epitopes on the S protein. The apparent critical functional and structural nature of this highly immunogenic determinant suggests a potential contribution in developing protective, cross-reactive subunit vaccines to IBV. |
| doi_str_mv | 10.1007/BF01323240 |
| format | Article |
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L ; KING, D. J ; COLLISSON, E. W</creator><creatorcontrib>WANG, L ; PARR, R. L ; KING, D. J ; COLLISSON, E. W</creatorcontrib><description>The predicted amino acid sequence and secondary structures of S1 of the spike protein (S) of infectious bronchitis viral (IBV) strains from Europe, the U.S.A., and Japan were compared. An antigenic determinant that was highly conserved in both the primary amino acid sequence and secondary structure of all strains was identified between amino acid positions 240 to 255. A synthesized peptide corresponding to this region was found to react with all polyclonal antisera examined from various IBV strains and with one monoclonal antibody (MAb), 9B1B6, out of nine known to react with the S of Gray. The specificity of the interaction with MAb 9B1B6 was confirmed by competitive ELISA using bound and unbound peptide. Interestingly, the previously described epitope for 9B1B6 had been characterized as cross-reactive with several strains of IBV, as conformation-independent but reacting only with intact whole S, and as associated with the functional integrity of other epitopes, including neutralizing epitopes on the S protein. The apparent critical functional and structural nature of this highly immunogenic determinant suggests a potential contribution in developing protective, cross-reactive subunit vaccines to IBV.</description><identifier>ISSN: 0304-8608</identifier><identifier>EISSN: 1432-8798</identifier><identifier>DOI: 10.1007/BF01323240</identifier><identifier>PMID: 8572941</identifier><language>eng</language><publisher>Wien: Springer</publisher><subject>Amino Acid Sequence ; Animals ; Antibodies, Monoclonal ; Biological and medical sciences ; Chickens ; Conserved Sequence ; Cross Reactions ; Enzyme-Linked Immunosorbent Assay ; Epitopes - analysis ; Europe ; Fundamental and applied biological sciences. Psychology ; Hemagglutinins, Viral - genetics ; Hemagglutinins, Viral - immunology ; Immunoblotting ; Infectious bronchitis virus - genetics ; Infectious bronchitis virus - immunology ; Japan ; Membrane Glycoproteins - analysis ; Membrane Glycoproteins - genetics ; Membrane Glycoproteins - immunology ; Microbiology ; Molecular Sequence Data ; Morphology, structure, chemical composition, physicochemical properties ; Original Papers ; Protein Structure, Secondary ; Sequence Homology, Amino Acid ; Spike Glycoprotein, Coronavirus ; United States ; Viral Envelope Proteins - analysis ; Viral Envelope Proteins - genetics ; Viral Envelope Proteins - immunology ; Virology</subject><ispartof>Archives of virology, 1995-01, Vol.140 (12), p.2201-2213</ispartof><rights>1996 INIST-CNRS</rights><rights>Springer-Verlag 1995</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-e4ca2cf1b654bcc0c74e102870a7dbf68ba55c9acdbc9da80e3f9fdcf58737203</citedby><cites>FETCH-LOGICAL-c402t-e4ca2cf1b654bcc0c74e102870a7dbf68ba55c9acdbc9da80e3f9fdcf58737203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,778,782,883,27907,27908</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2960780$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8572941$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>WANG, L</creatorcontrib><creatorcontrib>PARR, R. L</creatorcontrib><creatorcontrib>KING, D. J</creatorcontrib><creatorcontrib>COLLISSON, E. W</creatorcontrib><title>A highly conserved epitope on the spike protein of infectious bronchitis virus</title><title>Archives of virology</title><addtitle>Arch Virol</addtitle><description>The predicted amino acid sequence and secondary structures of S1 of the spike protein (S) of infectious bronchitis viral (IBV) strains from Europe, the U.S.A., and Japan were compared. An antigenic determinant that was highly conserved in both the primary amino acid sequence and secondary structure of all strains was identified between amino acid positions 240 to 255. A synthesized peptide corresponding to this region was found to react with all polyclonal antisera examined from various IBV strains and with one monoclonal antibody (MAb), 9B1B6, out of nine known to react with the S of Gray. The specificity of the interaction with MAb 9B1B6 was confirmed by competitive ELISA using bound and unbound peptide. Interestingly, the previously described epitope for 9B1B6 had been characterized as cross-reactive with several strains of IBV, as conformation-independent but reacting only with intact whole S, and as associated with the functional integrity of other epitopes, including neutralizing epitopes on the S protein. The apparent critical functional and structural nature of this highly immunogenic determinant suggests a potential contribution in developing protective, cross-reactive subunit vaccines to IBV.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Biological and medical sciences</subject><subject>Chickens</subject><subject>Conserved Sequence</subject><subject>Cross Reactions</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Epitopes - analysis</subject><subject>Europe</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hemagglutinins, Viral - genetics</subject><subject>Hemagglutinins, Viral - immunology</subject><subject>Immunoblotting</subject><subject>Infectious bronchitis virus - genetics</subject><subject>Infectious bronchitis virus - immunology</subject><subject>Japan</subject><subject>Membrane Glycoproteins - analysis</subject><subject>Membrane Glycoproteins - genetics</subject><subject>Membrane Glycoproteins - immunology</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Morphology, structure, chemical composition, physicochemical properties</subject><subject>Original Papers</subject><subject>Protein Structure, Secondary</subject><subject>Sequence Homology, Amino Acid</subject><subject>Spike Glycoprotein, Coronavirus</subject><subject>United States</subject><subject>Viral Envelope Proteins - analysis</subject><subject>Viral Envelope Proteins - genetics</subject><subject>Viral Envelope Proteins - immunology</subject><subject>Virology</subject><issn>0304-8608</issn><issn>1432-8798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkEFr3DAQhUVJSTZpL70XdAg9BNyOJXklXwppaNpCaC7pWcjjUazGK7mSdyH_vi5ZtslpYN7HezOPsXc1fKwB9Kcv11BLIYWCV2xVKykqo1tzxFYgQVVmDeaEnZbyG2BZyOaYHZtGi1bVK_bzkg_hfhgfOaZYKO-o5zSFOU3EU-TzQLxM4YH4lNNMIfLkeYiecA5pW3iXU8QhzKHwXcjb8oa99m4s9HY_z9iv6693V9-rm9tvP64ubypUIOaKFDqBvu7WjeoQAbWiGoTR4HTf-bXpXNNg67DvsO2dAZK-9T36xmipBcgz9vnJd9p2G-qR4pzdaKccNi4_2uSCfanEMNj7tLMalhAtFoMPe4Oc_mypzHYTCtI4ukjLY1ZrA43QagEvnkDMqZRM_hBSg_3Xvv3f_gK_f37WAd3Xvejne90VdKPPLmIoB0y0a1hy5V8Ai46H</recordid><startdate>19950101</startdate><enddate>19950101</enddate><creator>WANG, L</creator><creator>PARR, R. L</creator><creator>KING, D. J</creator><creator>COLLISSON, E. W</creator><general>Springer</general><general>Springer-Verlag</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19950101</creationdate><title>A highly conserved epitope on the spike protein of infectious bronchitis virus</title><author>WANG, L ; PARR, R. L ; KING, D. J ; COLLISSON, E. W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-e4ca2cf1b654bcc0c74e102870a7dbf68ba55c9acdbc9da80e3f9fdcf58737203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Biological and medical sciences</topic><topic>Chickens</topic><topic>Conserved Sequence</topic><topic>Cross Reactions</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Epitopes - analysis</topic><topic>Europe</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hemagglutinins, Viral - genetics</topic><topic>Hemagglutinins, Viral - immunology</topic><topic>Immunoblotting</topic><topic>Infectious bronchitis virus - genetics</topic><topic>Infectious bronchitis virus - immunology</topic><topic>Japan</topic><topic>Membrane Glycoproteins - analysis</topic><topic>Membrane Glycoproteins - genetics</topic><topic>Membrane Glycoproteins - immunology</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Morphology, structure, chemical composition, physicochemical properties</topic><topic>Original Papers</topic><topic>Protein Structure, Secondary</topic><topic>Sequence Homology, Amino Acid</topic><topic>Spike Glycoprotein, Coronavirus</topic><topic>United States</topic><topic>Viral Envelope Proteins - analysis</topic><topic>Viral Envelope Proteins - genetics</topic><topic>Viral Envelope Proteins - immunology</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WANG, L</creatorcontrib><creatorcontrib>PARR, R. L</creatorcontrib><creatorcontrib>KING, D. J</creatorcontrib><creatorcontrib>COLLISSON, E. W</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Archives of virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>WANG, L</au><au>PARR, R. L</au><au>KING, D. J</au><au>COLLISSON, E. W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A highly conserved epitope on the spike protein of infectious bronchitis virus</atitle><jtitle>Archives of virology</jtitle><addtitle>Arch Virol</addtitle><date>1995-01-01</date><risdate>1995</risdate><volume>140</volume><issue>12</issue><spage>2201</spage><epage>2213</epage><pages>2201-2213</pages><issn>0304-8608</issn><eissn>1432-8798</eissn><abstract>The predicted amino acid sequence and secondary structures of S1 of the spike protein (S) of infectious bronchitis viral (IBV) strains from Europe, the U.S.A., and Japan were compared. An antigenic determinant that was highly conserved in both the primary amino acid sequence and secondary structure of all strains was identified between amino acid positions 240 to 255. A synthesized peptide corresponding to this region was found to react with all polyclonal antisera examined from various IBV strains and with one monoclonal antibody (MAb), 9B1B6, out of nine known to react with the S of Gray. The specificity of the interaction with MAb 9B1B6 was confirmed by competitive ELISA using bound and unbound peptide. Interestingly, the previously described epitope for 9B1B6 had been characterized as cross-reactive with several strains of IBV, as conformation-independent but reacting only with intact whole S, and as associated with the functional integrity of other epitopes, including neutralizing epitopes on the S protein. The apparent critical functional and structural nature of this highly immunogenic determinant suggests a potential contribution in developing protective, cross-reactive subunit vaccines to IBV.</abstract><cop>Wien</cop><cop>New York, NY</cop><pub>Springer</pub><pmid>8572941</pmid><doi>10.1007/BF01323240</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence Animals Antibodies, Monoclonal Biological and medical sciences Chickens Conserved Sequence Cross Reactions Enzyme-Linked Immunosorbent Assay Epitopes - analysis Europe Fundamental and applied biological sciences. Psychology Hemagglutinins, Viral - genetics Hemagglutinins, Viral - immunology Immunoblotting Infectious bronchitis virus - genetics Infectious bronchitis virus - immunology Japan Membrane Glycoproteins - analysis Membrane Glycoproteins - genetics Membrane Glycoproteins - immunology Microbiology Molecular Sequence Data Morphology, structure, chemical composition, physicochemical properties Original Papers Protein Structure, Secondary Sequence Homology, Amino Acid Spike Glycoprotein, Coronavirus United States Viral Envelope Proteins - analysis Viral Envelope Proteins - genetics Viral Envelope Proteins - immunology Virology |
| title | A highly conserved epitope on the spike protein of infectious bronchitis virus |
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