Kinetics of humoral immune response to the major structural proteins of the porcine reproductive and respiratory syndrome virus
The kinetics of appearance of antibodies directed to the major structural proteins N, M and E of porcine reproductive and respiratory syndrome virus (PRRSV) was followed in pigs naturally- and experimentally-exposed to the virus. Specific IgM antibody titers were first detected by indirect immunoflu...
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| Veröffentlicht in: | Archives of virology 1996-01, Vol.141 (3/4), p.751-761 |
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| creator | Loemba, H.D Mounir, S Mardassi, H Archambault, D Dea, S |
| description | The kinetics of appearance of antibodies directed to the major structural proteins N, M and E of porcine reproductive and respiratory syndrome virus (PRRSV) was followed in pigs naturally- and experimentally-exposed to the virus. Specific IgM antibody titers were first detected by indirect immunofluorescence (IIF) at the end of the first week of PRRSV infection, peaked by day 14 to 21 post-inoculation (p.i.), then rapidly decreased to undetectable levels by day 35 to 42 p.i. On the other hand, specific IgG antibody titers peaked by day 21 to 28 p.i. and remained unchanged to the end of the 6- or 9-week observation period; in addition, a persistent viremia was observed. Virus neutralizing (VN) antibody titers > 8 were not detected until 3 to 4 weeks p.i. Taken together, the results obtained by Western blotting analyses using purified virus and E. coli-expressed ORFs 5 to 7 gene products, suggested that antibodies directed against the envelope E protein appear by day 7 p.i., whereas antibodies directed against the nucleocapsid N and membrane M proteins can only be detected by the end of the second week p.i. No correlation could be demonstrated between VN and IIF antibody titers, viremia, and viral protein specificities of circulating antibodies at various times p.i. |
| doi_str_mv | 10.1007/BF01718333 |
| format | Article |
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Specific IgM antibody titers were first detected by indirect immunofluorescence (IIF) at the end of the first week of PRRSV infection, peaked by day 14 to 21 post-inoculation (p.i.), then rapidly decreased to undetectable levels by day 35 to 42 p.i. On the other hand, specific IgG antibody titers peaked by day 21 to 28 p.i. and remained unchanged to the end of the 6- or 9-week observation period; in addition, a persistent viremia was observed. Virus neutralizing (VN) antibody titers > 8 were not detected until 3 to 4 weeks p.i. Taken together, the results obtained by Western blotting analyses using purified virus and E. coli-expressed ORFs 5 to 7 gene products, suggested that antibodies directed against the envelope E protein appear by day 7 p.i., whereas antibodies directed against the nucleocapsid N and membrane M proteins can only be detected by the end of the second week p.i. No correlation could be demonstrated between VN and IIF antibody titers, viremia, and viral protein specificities of circulating antibodies at various times p.i.</description><identifier>ISSN: 0304-8608</identifier><identifier>EISSN: 1432-8798</identifier><identifier>DOI: 10.1007/BF01718333</identifier><identifier>PMID: 8645111</identifier><language>eng</language><publisher>Wien: Springer</publisher><subject>Analysis of the immune response. Humoral and cellular immunity ; Animals ; Antibodies, Viral - biosynthesis ; Antibodies, Viral - immunology ; Antibody Formation ; Antibody production ; Arterivirus ; Arterivirus - immunology ; Arterivirus Infections - immunology ; Arterivirus Infections - pathology ; Arterivirus Infections - virology ; Biological and medical sciences ; Blotting, Western ; Brief Report ; Cell Line ; duration ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; fusion proteins ; Immunobiology ; immunoglobulin G ; Immunoglobulin G - biosynthesis ; Immunoglobulin G - immunology ; immunoglobulin M ; Immunoglobulin M - biosynthesis ; Immunoglobulin M - immunology ; Kinetics ; major structural proteins ; Molecular Sequence Data ; Swine ; swine diseases ; Swine Diseases - immunology ; Swine Diseases - pathology ; Swine Diseases - virology ; symptoms ; viral proteins ; Viral Structural Proteins - immunology ; virus neutralization</subject><ispartof>Archives of virology, 1996-01, Vol.141 (3/4), p.751-761</ispartof><rights>1996 INIST-CNRS</rights><rights>Springer-Verlag 1996</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-dd9ab561fb0e616ec130dc959cf48197ab70b8df3a178476d48c4a5661477063</citedby><cites>FETCH-LOGICAL-c457t-dd9ab561fb0e616ec130dc959cf48197ab70b8df3a178476d48c4a5661477063</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3061596$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8645111$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Loemba, H.D</creatorcontrib><creatorcontrib>Mounir, S</creatorcontrib><creatorcontrib>Mardassi, H</creatorcontrib><creatorcontrib>Archambault, D</creatorcontrib><creatorcontrib>Dea, S</creatorcontrib><title>Kinetics of humoral immune response to the major structural proteins of the porcine reproductive and respiratory syndrome virus</title><title>Archives of virology</title><addtitle>Arch Virol</addtitle><description>The kinetics of appearance of antibodies directed to the major structural proteins N, M and E of porcine reproductive and respiratory syndrome virus (PRRSV) was followed in pigs naturally- and experimentally-exposed to the virus. Specific IgM antibody titers were first detected by indirect immunofluorescence (IIF) at the end of the first week of PRRSV infection, peaked by day 14 to 21 post-inoculation (p.i.), then rapidly decreased to undetectable levels by day 35 to 42 p.i. On the other hand, specific IgG antibody titers peaked by day 21 to 28 p.i. and remained unchanged to the end of the 6- or 9-week observation period; in addition, a persistent viremia was observed. Virus neutralizing (VN) antibody titers > 8 were not detected until 3 to 4 weeks p.i. Taken together, the results obtained by Western blotting analyses using purified virus and E. coli-expressed ORFs 5 to 7 gene products, suggested that antibodies directed against the envelope E protein appear by day 7 p.i., whereas antibodies directed against the nucleocapsid N and membrane M proteins can only be detected by the end of the second week p.i. No correlation could be demonstrated between VN and IIF antibody titers, viremia, and viral protein specificities of circulating antibodies at various times p.i.</description><subject>Analysis of the immune response. Humoral and cellular immunity</subject><subject>Animals</subject><subject>Antibodies, Viral - biosynthesis</subject><subject>Antibodies, Viral - immunology</subject><subject>Antibody Formation</subject><subject>Antibody production</subject><subject>Arterivirus</subject><subject>Arterivirus - immunology</subject><subject>Arterivirus Infections - immunology</subject><subject>Arterivirus Infections - pathology</subject><subject>Arterivirus Infections - virology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Brief Report</subject><subject>Cell Line</subject><subject>duration</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>fusion proteins</subject><subject>Immunobiology</subject><subject>immunoglobulin G</subject><subject>Immunoglobulin G - biosynthesis</subject><subject>Immunoglobulin G - immunology</subject><subject>immunoglobulin M</subject><subject>Immunoglobulin M - biosynthesis</subject><subject>Immunoglobulin M - immunology</subject><subject>Kinetics</subject><subject>major structural proteins</subject><subject>Molecular Sequence Data</subject><subject>Swine</subject><subject>swine diseases</subject><subject>Swine Diseases - immunology</subject><subject>Swine Diseases - pathology</subject><subject>Swine Diseases - virology</subject><subject>symptoms</subject><subject>viral proteins</subject><subject>Viral Structural Proteins - immunology</subject><subject>virus neutralization</subject><issn>0304-8608</issn><issn>1432-8798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxS0EKtvChTvCB9QDUmC8_sylElQUEJU4UM6W4zhdV0kcbGelPfGv4-yuFjhxsuT3m6eZ9xB6QeAtAZDvPtwAkURRSh-hFWF0XSlZq8doBRRYpQSop-g8pQeA8kH5GTpTgnFCyAr9-upHl71NOHR4Mw8hmh77YZhHh6NLUxiTwzngvHF4MA8h4pTjbPO8cFMM2flxP7sAU4jW7weL0hbKbx02Y7t38tHkEHc47cY2hsHhrY9zeoaedKZP7vnxvUB3Nx_vrj9Xt98-fbl-f1tZxmWu2rY2DReka8AJIpwlFFpb89p2TJFamkZCo9qOGiIVk6JlyjLDhSBMShD0Al0dbKe5GVxr3ZjLAXqKfjBxp4Px-l9l9Bt9H7ZaghI1o8Xg8mgQw8_ZpawHn6zrezO6MCctFVDBS7r_AwnnisF6Ad8cQBtDStF1p20I6KVW_afWAr_8e_8Teuyx6K-PuknW9F00o_XphFEQhNdLDK8OWGeCNvexID--r6GESfgaJGP0N7B-to0</recordid><startdate>19960101</startdate><enddate>19960101</enddate><creator>Loemba, H.D</creator><creator>Mounir, S</creator><creator>Mardassi, H</creator><creator>Archambault, D</creator><creator>Dea, S</creator><general>Springer</general><general>Springer-Verlag</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19960101</creationdate><title>Kinetics of humoral immune response to the major structural proteins of the porcine reproductive and respiratory syndrome virus</title><author>Loemba, H.D ; Mounir, S ; Mardassi, H ; Archambault, D ; Dea, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-dd9ab561fb0e616ec130dc959cf48197ab70b8df3a178476d48c4a5661477063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Analysis of the immune response. Humoral and cellular immunity</topic><topic>Animals</topic><topic>Antibodies, Viral - biosynthesis</topic><topic>Antibodies, Viral - immunology</topic><topic>Antibody Formation</topic><topic>Antibody production</topic><topic>Arterivirus</topic><topic>Arterivirus - immunology</topic><topic>Arterivirus Infections - immunology</topic><topic>Arterivirus Infections - pathology</topic><topic>Arterivirus Infections - virology</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Brief Report</topic><topic>Cell Line</topic><topic>duration</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>fusion proteins</topic><topic>Immunobiology</topic><topic>immunoglobulin G</topic><topic>Immunoglobulin G - biosynthesis</topic><topic>Immunoglobulin G - immunology</topic><topic>immunoglobulin M</topic><topic>Immunoglobulin M - biosynthesis</topic><topic>Immunoglobulin M - immunology</topic><topic>Kinetics</topic><topic>major structural proteins</topic><topic>Molecular Sequence Data</topic><topic>Swine</topic><topic>swine diseases</topic><topic>Swine Diseases - immunology</topic><topic>Swine Diseases - pathology</topic><topic>Swine Diseases - virology</topic><topic>symptoms</topic><topic>viral proteins</topic><topic>Viral Structural Proteins - immunology</topic><topic>virus neutralization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Loemba, H.D</creatorcontrib><creatorcontrib>Mounir, S</creatorcontrib><creatorcontrib>Mardassi, H</creatorcontrib><creatorcontrib>Archambault, D</creatorcontrib><creatorcontrib>Dea, S</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Archives of virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Loemba, H.D</au><au>Mounir, S</au><au>Mardassi, H</au><au>Archambault, D</au><au>Dea, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Kinetics of humoral immune response to the major structural proteins of the porcine reproductive and respiratory syndrome virus</atitle><jtitle>Archives of virology</jtitle><addtitle>Arch Virol</addtitle><date>1996-01-01</date><risdate>1996</risdate><volume>141</volume><issue>3/4</issue><spage>751</spage><epage>761</epage><pages>751-761</pages><issn>0304-8608</issn><eissn>1432-8798</eissn><abstract>The kinetics of appearance of antibodies directed to the major structural proteins N, M and E of porcine reproductive and respiratory syndrome virus (PRRSV) was followed in pigs naturally- and experimentally-exposed to the virus. Specific IgM antibody titers were first detected by indirect immunofluorescence (IIF) at the end of the first week of PRRSV infection, peaked by day 14 to 21 post-inoculation (p.i.), then rapidly decreased to undetectable levels by day 35 to 42 p.i. On the other hand, specific IgG antibody titers peaked by day 21 to 28 p.i. and remained unchanged to the end of the 6- or 9-week observation period; in addition, a persistent viremia was observed. Virus neutralizing (VN) antibody titers > 8 were not detected until 3 to 4 weeks p.i. Taken together, the results obtained by Western blotting analyses using purified virus and E. coli-expressed ORFs 5 to 7 gene products, suggested that antibodies directed against the envelope E protein appear by day 7 p.i., whereas antibodies directed against the nucleocapsid N and membrane M proteins can only be detected by the end of the second week p.i. No correlation could be demonstrated between VN and IIF antibody titers, viremia, and viral protein specificities of circulating antibodies at various times p.i.</abstract><cop>Wien</cop><cop>New York, NY</cop><pub>Springer</pub><pmid>8645111</pmid><doi>10.1007/BF01718333</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Archives of virology, 1996-01, Vol.141 (3/4), p.751-761 |
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| subjects | Analysis of the immune response. Humoral and cellular immunity Animals Antibodies, Viral - biosynthesis Antibodies, Viral - immunology Antibody Formation Antibody production Arterivirus Arterivirus - immunology Arterivirus Infections - immunology Arterivirus Infections - pathology Arterivirus Infections - virology Biological and medical sciences Blotting, Western Brief Report Cell Line duration Fundamental and applied biological sciences. Psychology Fundamental immunology fusion proteins Immunobiology immunoglobulin G Immunoglobulin G - biosynthesis Immunoglobulin G - immunology immunoglobulin M Immunoglobulin M - biosynthesis Immunoglobulin M - immunology Kinetics major structural proteins Molecular Sequence Data Swine swine diseases Swine Diseases - immunology Swine Diseases - pathology Swine Diseases - virology symptoms viral proteins Viral Structural Proteins - immunology virus neutralization |
| title | Kinetics of humoral immune response to the major structural proteins of the porcine reproductive and respiratory syndrome virus |
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