A moonlighting nuclease puts CRISPR in its place
Integration of spacers into CRISPR loci requires the Cas1/Cas2 integrase complex, frequently in combination with Cas4 exonuclease. However, several CRISPR-Cas systems lack Cas4. Whether Cas4-like activity is dispensable in these systems or provided by an unidentified actor was not known. In this iss...
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| Veröffentlicht in: | The Journal of biological chemistry 2020-03, Vol.295 (11), p.3415-3416 |
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| container_title | The Journal of biological chemistry |
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| creator | Lawrence, C. Martin |
| description | Integration of spacers into CRISPR loci requires the Cas1/Cas2 integrase complex, frequently in combination with Cas4 exonuclease. However, several CRISPR-Cas systems lack Cas4. Whether Cas4-like activity is dispensable in these systems or provided by an unidentified actor was not known. In this issue of the Journal of Biological Chemistry, Ramachandran et al. show that in subtype I-E systems, Cas4-like activity is supplied by DnaQ-superfamily exonucleases, providing a beautiful example of cellular machinery moonlighting in support of CRISPR-Cas adaptive immunity. |
| doi_str_mv | 10.1074/jbc.H120.012897 |
| format | Article |
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| issn | 0021-9258 1083-351X |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Clustered Regularly Interspaced Short Palindromic Repeats CRISPR-Associated Proteins - genetics CRISPR-Cas Systems DNA Polymerase III Editors' Picks Highlights Endonucleases - genetics Endonucleases - metabolism Escherichia coli - metabolism Escherichia coli Proteins - metabolism Exonucleases |
| title | A moonlighting nuclease puts CRISPR in its place |
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