Integrative analysis of gut microbiome and metabolites revealed novel mechanisms of intestinal Salmonella carriage in chicken
Intestinal carriage of Salmonella Enteritidis (SE) in the chicken host serves as a reservoir for transmission of Salmonella to humans through the consumption of poultry products. The aim of the current study was to examine the three-way interaction that occurred between host metabolites, resident gu...
Gespeichert in:
| Veröffentlicht in: | Scientific reports 2020-03, Vol.10 (1), p.4809-4809, Article 4809 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 4809 |
|---|---|
| container_issue | 1 |
| container_start_page | 4809 |
| container_title | Scientific reports |
| container_volume | 10 |
| creator | Mon, Khin K. Z. Zhu, Yuhua Chanthavixay, Ganrea Kern, Colin Zhou, Huaijun |
| description | Intestinal carriage of
Salmonella
Enteritidis (SE) in the chicken host serves as a reservoir for transmission of
Salmonella
to humans through the consumption of poultry products. The aim of the current study was to examine the three-way interaction that occurred between host metabolites, resident gut microbiota and
Salmonella
following inoculation of SE in two-week-old layer chicks. Our results revealed an overall alteration in gut microbiome and metabolites in association with SE infection. Enriched colonization by different microbial members throughout the course of experimental infection highlighted significant fluctuation in the intestinal microbial community in response to
Salmonella
infection. As changes in community membership occurred, there was also subsequent impact on differential regulation of interlinked predicted functional activities within the intestinal environment dictated by
Salmonella
-commensal interaction. Alteration in the overall microbial community following infection also has a ripple effect on the host regulation of cecum-associated metabolic networks. The findings showed that there was differential regulation in many of the metabolites in association with SE colonization in chickens. Perturbation in metabolic pathways related to arginine and proline metabolism as well as TCA cycle was most prominently detected. Taken together, the present findings provided a starting point in understanding the effect of intestinal
Salmonella
carriage on the microbiome and metabolome of developing young layer chicks. |
| doi_str_mv | 10.1038/s41598-020-60892-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7075953</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2377672504</sourcerecordid><originalsourceid>FETCH-LOGICAL-c511t-2bef15be7b34940705fd3cfb39cbf54ef8f96a1fe1760d4d3f52a26e0d6510d73</originalsourceid><addsrcrecordid>eNp9kU9PFTEUxSdGIgT5AixMEzduRvt3-roxMUSBhMSFsG46ndt5xU6L7cxLWPjd7WMQ0YXdtMn53XN772maU4LfE8w2HwonQm1aTHHb4Y2irXrRHFHMRUsZpS-fvQ-bk1JucT2CKk7Uq-aQUSKVFPyo-XkZZxizmf0OkIkm3BdfUHJoXGY0eZtT79O0lwY0wWz6FPwMBWXYgQkwoJh2EKpktyb6Mj3U-upZZl_d0DcTphQhBIOsydmbEaqM7Nbb7xBfNwfOhAInj_dxc_Pl8_XZRXv19fzy7NNVawUhc0t7cET0IHvGFccSCzcw63qmbO8EB7dxqjPEAZEdHvjAnKCGdoCHThA8SHbcfFx975Z-gsFCnLMJ-i77yeR7nYzXfyvRb_WYdlpiKZRg1eDdo0FOP5Y6nJ58sfuxIqSlaMqkVEoIiiv69h_0Ni257mKlOkkF5pWiK1U3XEoG9_QZgvU-YL0GrGvA-iFgrWrRm-djPJX8jrMCbAVKleII-U_v_9j-AvZxtIg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2377672504</pqid></control><display><type>article</type><title>Integrative analysis of gut microbiome and metabolites revealed novel mechanisms of intestinal Salmonella carriage in chicken</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Springer Nature OA Free Journals</source><source>Nature Free</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Mon, Khin K. Z. ; Zhu, Yuhua ; Chanthavixay, Ganrea ; Kern, Colin ; Zhou, Huaijun</creator><creatorcontrib>Mon, Khin K. Z. ; Zhu, Yuhua ; Chanthavixay, Ganrea ; Kern, Colin ; Zhou, Huaijun</creatorcontrib><description>Intestinal carriage of
Salmonella
Enteritidis (SE) in the chicken host serves as a reservoir for transmission of
Salmonella
to humans through the consumption of poultry products. The aim of the current study was to examine the three-way interaction that occurred between host metabolites, resident gut microbiota and
Salmonella
following inoculation of SE in two-week-old layer chicks. Our results revealed an overall alteration in gut microbiome and metabolites in association with SE infection. Enriched colonization by different microbial members throughout the course of experimental infection highlighted significant fluctuation in the intestinal microbial community in response to
Salmonella
infection. As changes in community membership occurred, there was also subsequent impact on differential regulation of interlinked predicted functional activities within the intestinal environment dictated by
Salmonella
-commensal interaction. Alteration in the overall microbial community following infection also has a ripple effect on the host regulation of cecum-associated metabolic networks. The findings showed that there was differential regulation in many of the metabolites in association with SE colonization in chickens. Perturbation in metabolic pathways related to arginine and proline metabolism as well as TCA cycle was most prominently detected. Taken together, the present findings provided a starting point in understanding the effect of intestinal
Salmonella
carriage on the microbiome and metabolome of developing young layer chicks.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-020-60892-9</identifier><identifier>PMID: 32179754</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/326/2565/2134 ; 631/326/421 ; Animals ; Arginine ; Arginine - metabolism ; Cecum ; Cecum - metabolism ; Chickens ; Chickens - metabolism ; Chickens - microbiology ; Colonization ; Digestive system ; Experimental infection ; Gastrointestinal Microbiome ; Gastrointestinal tract ; Host-Pathogen Interactions ; Humanities and Social Sciences ; Infections ; Inoculation ; Intestinal microflora ; Intestine ; Juveniles ; Metabolic networks ; Metabolic pathways ; Metabolism ; Metabolites ; Microbial Interactions ; Microbiomes ; Microbiota ; multidisciplinary ; Proline ; Proline - metabolism ; Salmonella ; Salmonella enteritidis ; Salmonella Infections, Animal - metabolism ; Salmonella Infections, Animal - microbiology ; Science ; Science (multidisciplinary) ; Tricarboxylic acid cycle</subject><ispartof>Scientific reports, 2020-03, Vol.10 (1), p.4809-4809, Article 4809</ispartof><rights>The Author(s) 2020</rights><rights>This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c511t-2bef15be7b34940705fd3cfb39cbf54ef8f96a1fe1760d4d3f52a26e0d6510d73</citedby><cites>FETCH-LOGICAL-c511t-2bef15be7b34940705fd3cfb39cbf54ef8f96a1fe1760d4d3f52a26e0d6510d73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7075953/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7075953/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,27905,27906,41101,42170,51557,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32179754$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mon, Khin K. Z.</creatorcontrib><creatorcontrib>Zhu, Yuhua</creatorcontrib><creatorcontrib>Chanthavixay, Ganrea</creatorcontrib><creatorcontrib>Kern, Colin</creatorcontrib><creatorcontrib>Zhou, Huaijun</creatorcontrib><title>Integrative analysis of gut microbiome and metabolites revealed novel mechanisms of intestinal Salmonella carriage in chicken</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Intestinal carriage of
Salmonella
Enteritidis (SE) in the chicken host serves as a reservoir for transmission of
Salmonella
to humans through the consumption of poultry products. The aim of the current study was to examine the three-way interaction that occurred between host metabolites, resident gut microbiota and
Salmonella
following inoculation of SE in two-week-old layer chicks. Our results revealed an overall alteration in gut microbiome and metabolites in association with SE infection. Enriched colonization by different microbial members throughout the course of experimental infection highlighted significant fluctuation in the intestinal microbial community in response to
Salmonella
infection. As changes in community membership occurred, there was also subsequent impact on differential regulation of interlinked predicted functional activities within the intestinal environment dictated by
Salmonella
-commensal interaction. Alteration in the overall microbial community following infection also has a ripple effect on the host regulation of cecum-associated metabolic networks. The findings showed that there was differential regulation in many of the metabolites in association with SE colonization in chickens. Perturbation in metabolic pathways related to arginine and proline metabolism as well as TCA cycle was most prominently detected. Taken together, the present findings provided a starting point in understanding the effect of intestinal
Salmonella
carriage on the microbiome and metabolome of developing young layer chicks.</description><subject>631/326/2565/2134</subject><subject>631/326/421</subject><subject>Animals</subject><subject>Arginine</subject><subject>Arginine - metabolism</subject><subject>Cecum</subject><subject>Cecum - metabolism</subject><subject>Chickens</subject><subject>Chickens - metabolism</subject><subject>Chickens - microbiology</subject><subject>Colonization</subject><subject>Digestive system</subject><subject>Experimental infection</subject><subject>Gastrointestinal Microbiome</subject><subject>Gastrointestinal tract</subject><subject>Host-Pathogen Interactions</subject><subject>Humanities and Social Sciences</subject><subject>Infections</subject><subject>Inoculation</subject><subject>Intestinal microflora</subject><subject>Intestine</subject><subject>Juveniles</subject><subject>Metabolic networks</subject><subject>Metabolic pathways</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Microbial Interactions</subject><subject>Microbiomes</subject><subject>Microbiota</subject><subject>multidisciplinary</subject><subject>Proline</subject><subject>Proline - metabolism</subject><subject>Salmonella</subject><subject>Salmonella enteritidis</subject><subject>Salmonella Infections, Animal - metabolism</subject><subject>Salmonella Infections, Animal - microbiology</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Tricarboxylic acid cycle</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU9PFTEUxSdGIgT5AixMEzduRvt3-roxMUSBhMSFsG46ndt5xU6L7cxLWPjd7WMQ0YXdtMn53XN772maU4LfE8w2HwonQm1aTHHb4Y2irXrRHFHMRUsZpS-fvQ-bk1JucT2CKk7Uq-aQUSKVFPyo-XkZZxizmf0OkIkm3BdfUHJoXGY0eZtT79O0lwY0wWz6FPwMBWXYgQkwoJh2EKpktyb6Mj3U-upZZl_d0DcTphQhBIOsydmbEaqM7Nbb7xBfNwfOhAInj_dxc_Pl8_XZRXv19fzy7NNVawUhc0t7cET0IHvGFccSCzcw63qmbO8EB7dxqjPEAZEdHvjAnKCGdoCHThA8SHbcfFx975Z-gsFCnLMJ-i77yeR7nYzXfyvRb_WYdlpiKZRg1eDdo0FOP5Y6nJ58sfuxIqSlaMqkVEoIiiv69h_0Ni257mKlOkkF5pWiK1U3XEoG9_QZgvU-YL0GrGvA-iFgrWrRm-djPJX8jrMCbAVKleII-U_v_9j-AvZxtIg</recordid><startdate>20200316</startdate><enddate>20200316</enddate><creator>Mon, Khin K. Z.</creator><creator>Zhu, Yuhua</creator><creator>Chanthavixay, Ganrea</creator><creator>Kern, Colin</creator><creator>Zhou, Huaijun</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20200316</creationdate><title>Integrative analysis of gut microbiome and metabolites revealed novel mechanisms of intestinal Salmonella carriage in chicken</title><author>Mon, Khin K. Z. ; Zhu, Yuhua ; Chanthavixay, Ganrea ; Kern, Colin ; Zhou, Huaijun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c511t-2bef15be7b34940705fd3cfb39cbf54ef8f96a1fe1760d4d3f52a26e0d6510d73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>631/326/2565/2134</topic><topic>631/326/421</topic><topic>Animals</topic><topic>Arginine</topic><topic>Arginine - metabolism</topic><topic>Cecum</topic><topic>Cecum - metabolism</topic><topic>Chickens</topic><topic>Chickens - metabolism</topic><topic>Chickens - microbiology</topic><topic>Colonization</topic><topic>Digestive system</topic><topic>Experimental infection</topic><topic>Gastrointestinal Microbiome</topic><topic>Gastrointestinal tract</topic><topic>Host-Pathogen Interactions</topic><topic>Humanities and Social Sciences</topic><topic>Infections</topic><topic>Inoculation</topic><topic>Intestinal microflora</topic><topic>Intestine</topic><topic>Juveniles</topic><topic>Metabolic networks</topic><topic>Metabolic pathways</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Microbial Interactions</topic><topic>Microbiomes</topic><topic>Microbiota</topic><topic>multidisciplinary</topic><topic>Proline</topic><topic>Proline - metabolism</topic><topic>Salmonella</topic><topic>Salmonella enteritidis</topic><topic>Salmonella Infections, Animal - metabolism</topic><topic>Salmonella Infections, Animal - microbiology</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Tricarboxylic acid cycle</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mon, Khin K. Z.</creatorcontrib><creatorcontrib>Zhu, Yuhua</creatorcontrib><creatorcontrib>Chanthavixay, Ganrea</creatorcontrib><creatorcontrib>Kern, Colin</creatorcontrib><creatorcontrib>Zhou, Huaijun</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mon, Khin K. Z.</au><au>Zhu, Yuhua</au><au>Chanthavixay, Ganrea</au><au>Kern, Colin</au><au>Zhou, Huaijun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Integrative analysis of gut microbiome and metabolites revealed novel mechanisms of intestinal Salmonella carriage in chicken</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2020-03-16</date><risdate>2020</risdate><volume>10</volume><issue>1</issue><spage>4809</spage><epage>4809</epage><pages>4809-4809</pages><artnum>4809</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Intestinal carriage of
Salmonella
Enteritidis (SE) in the chicken host serves as a reservoir for transmission of
Salmonella
to humans through the consumption of poultry products. The aim of the current study was to examine the three-way interaction that occurred between host metabolites, resident gut microbiota and
Salmonella
following inoculation of SE in two-week-old layer chicks. Our results revealed an overall alteration in gut microbiome and metabolites in association with SE infection. Enriched colonization by different microbial members throughout the course of experimental infection highlighted significant fluctuation in the intestinal microbial community in response to
Salmonella
infection. As changes in community membership occurred, there was also subsequent impact on differential regulation of interlinked predicted functional activities within the intestinal environment dictated by
Salmonella
-commensal interaction. Alteration in the overall microbial community following infection also has a ripple effect on the host regulation of cecum-associated metabolic networks. The findings showed that there was differential regulation in many of the metabolites in association with SE colonization in chickens. Perturbation in metabolic pathways related to arginine and proline metabolism as well as TCA cycle was most prominently detected. Taken together, the present findings provided a starting point in understanding the effect of intestinal
Salmonella
carriage on the microbiome and metabolome of developing young layer chicks.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>32179754</pmid><doi>10.1038/s41598-020-60892-9</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2045-2322 |
| ispartof | Scientific reports, 2020-03, Vol.10 (1), p.4809-4809, Article 4809 |
| issn | 2045-2322 2045-2322 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7075953 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Springer Nature OA Free Journals; Nature Free; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | 631/326/2565/2134 631/326/421 Animals Arginine Arginine - metabolism Cecum Cecum - metabolism Chickens Chickens - metabolism Chickens - microbiology Colonization Digestive system Experimental infection Gastrointestinal Microbiome Gastrointestinal tract Host-Pathogen Interactions Humanities and Social Sciences Infections Inoculation Intestinal microflora Intestine Juveniles Metabolic networks Metabolic pathways Metabolism Metabolites Microbial Interactions Microbiomes Microbiota multidisciplinary Proline Proline - metabolism Salmonella Salmonella enteritidis Salmonella Infections, Animal - metabolism Salmonella Infections, Animal - microbiology Science Science (multidisciplinary) Tricarboxylic acid cycle |
| title | Integrative analysis of gut microbiome and metabolites revealed novel mechanisms of intestinal Salmonella carriage in chicken |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T11%3A54%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Integrative%20analysis%20of%20gut%20microbiome%20and%20metabolites%20revealed%20novel%20mechanisms%20of%20intestinal%20Salmonella%20carriage%20in%20chicken&rft.jtitle=Scientific%20reports&rft.au=Mon,%20Khin%20K.%20Z.&rft.date=2020-03-16&rft.volume=10&rft.issue=1&rft.spage=4809&rft.epage=4809&rft.pages=4809-4809&rft.artnum=4809&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/s41598-020-60892-9&rft_dat=%3Cproquest_pubme%3E2377672504%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2377672504&rft_id=info:pmid/32179754&rfr_iscdi=true |