Induction of a Specific Humoral Immune Response by Nasal Delivery of Bcla2ctd of Clostridioides difficile

Clostridioides difficile, formerly known as Clostridium difficile, is a spore-forming bacterium considered as the most common cause of nosocomial infections in developed countries. The spore of C. difficile is involved in the transmission of the pathogen and in its first interaction with the host; t...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:International journal of molecular sciences 2020-02, Vol.21 (4), p.1277
Hauptverfasser: Maia, Ana Raquel, Reyes-Ramírez, Rodrigo, Pizarro-Guajardo, Marjorie, Saggese, Anella, Castro-Córdova, Pablo, Isticato, Rachele, Ricca, Ezio, Paredes-Sabja, Daniel, Baccigalupi, Loredana
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue 4
container_start_page 1277
container_title International journal of molecular sciences
container_volume 21
creator Maia, Ana Raquel
Reyes-Ramírez, Rodrigo
Pizarro-Guajardo, Marjorie
Saggese, Anella
Castro-Córdova, Pablo
Isticato, Rachele
Ricca, Ezio
Paredes-Sabja, Daniel
Baccigalupi, Loredana
description Clostridioides difficile, formerly known as Clostridium difficile, is a spore-forming bacterium considered as the most common cause of nosocomial infections in developed countries. The spore of C. difficile is involved in the transmission of the pathogen and in its first interaction with the host; therefore, a therapeutic approach able to control C. difficile spores would improve the clearance of the infection. The C-terminal (CTD) end of BclA2, a spore surface protein of C. difficile responsible of the interaction with the host intestinal cells, was selected as a putative mucosal antigen. The BclA2 fragment, BclA2CTD, was purified and used to nasally immunize mice both as a free protein and after adsorption to the spore of Bacillus subtilis, a well-established mucosal delivery vehicle. While the adsorption to spores increased the in vitro stability of BclA2CTD, in vivo both free and spore-adsorbed BclA2CTD were able to induce a similar, specific humoral immune response in a murine model. Although in the experimental conditions utilized the immune response was not protective, the induction of specific IgG indicates that free or spore-bound BclA2CTD could act as a putative mucosal antigen targeting C. difficile spores.
doi_str_mv 10.3390/ijms21041277
format Article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7072882</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2359417330</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2767-3c0e0f85554237df940eb44a1b00ecd7a0454400bedf78a6f74269197f5ef9cd3</originalsourceid><addsrcrecordid>eNpVkVtLAzEQhYMoVqtv_oA8-mB1Npem-yJovRWKgpfnkE0mmrK7qZvdQv-9WyxSn-bAOfMNwyHkLINLznO4CosqsQxExpTaI0eZYGwEMFb7O3pAjlNaADDOZH5IBpyBErmURyTMatfZNsSaRk8NfVuiDT5Y-tRVsTElnVVVVyN9xbSMdUJarOmzSb1xh2VYYbPe7N3a0jDbuo2eljG1TXAhBoeJuuB7XCjxhBx4UyY83c4h-Xi4f58-jeYvj7PpzXxkmRqrEbeA4CdSSsG4cj4XgIUQJisA0DplQEghAAp0Xk3M2CvBxnmWKy_R59bxIbn-5S67okJnsW77P_SyCZVp1jqaoP87dfjSn3GlFSg2mbAecL4FNPG7w9TqKiSLZWlqjF3SjMtcZIpz6KMXv1HbxJQa9H9nMtCbdvRuO_wHLc6CrQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2359417330</pqid></control><display><type>article</type><title>Induction of a Specific Humoral Immune Response by Nasal Delivery of Bcla2ctd of Clostridioides difficile</title><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>Maia, Ana Raquel ; Reyes-Ramírez, Rodrigo ; Pizarro-Guajardo, Marjorie ; Saggese, Anella ; Castro-Córdova, Pablo ; Isticato, Rachele ; Ricca, Ezio ; Paredes-Sabja, Daniel ; Baccigalupi, Loredana</creator><creatorcontrib>Maia, Ana Raquel ; Reyes-Ramírez, Rodrigo ; Pizarro-Guajardo, Marjorie ; Saggese, Anella ; Castro-Córdova, Pablo ; Isticato, Rachele ; Ricca, Ezio ; Paredes-Sabja, Daniel ; Baccigalupi, Loredana</creatorcontrib><description>Clostridioides difficile, formerly known as Clostridium difficile, is a spore-forming bacterium considered as the most common cause of nosocomial infections in developed countries. The spore of C. difficile is involved in the transmission of the pathogen and in its first interaction with the host; therefore, a therapeutic approach able to control C. difficile spores would improve the clearance of the infection. The C-terminal (CTD) end of BclA2, a spore surface protein of C. difficile responsible of the interaction with the host intestinal cells, was selected as a putative mucosal antigen. The BclA2 fragment, BclA2CTD, was purified and used to nasally immunize mice both as a free protein and after adsorption to the spore of Bacillus subtilis, a well-established mucosal delivery vehicle. While the adsorption to spores increased the in vitro stability of BclA2CTD, in vivo both free and spore-adsorbed BclA2CTD were able to induce a similar, specific humoral immune response in a murine model. Although in the experimental conditions utilized the immune response was not protective, the induction of specific IgG indicates that free or spore-bound BclA2CTD could act as a putative mucosal antigen targeting C. difficile spores.</description><identifier>ISSN: 1422-0067</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms21041277</identifier><identifier>PMID: 32074955</identifier><language>eng</language><publisher>MDPI</publisher><ispartof>International journal of molecular sciences, 2020-02, Vol.21 (4), p.1277</ispartof><rights>2020 by the authors. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2767-3c0e0f85554237df940eb44a1b00ecd7a0454400bedf78a6f74269197f5ef9cd3</citedby><cites>FETCH-LOGICAL-c2767-3c0e0f85554237df940eb44a1b00ecd7a0454400bedf78a6f74269197f5ef9cd3</cites><orcidid>0000-0002-3370-4878 ; 0000-0002-6176-9943</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072882/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072882/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids></links><search><creatorcontrib>Maia, Ana Raquel</creatorcontrib><creatorcontrib>Reyes-Ramírez, Rodrigo</creatorcontrib><creatorcontrib>Pizarro-Guajardo, Marjorie</creatorcontrib><creatorcontrib>Saggese, Anella</creatorcontrib><creatorcontrib>Castro-Córdova, Pablo</creatorcontrib><creatorcontrib>Isticato, Rachele</creatorcontrib><creatorcontrib>Ricca, Ezio</creatorcontrib><creatorcontrib>Paredes-Sabja, Daniel</creatorcontrib><creatorcontrib>Baccigalupi, Loredana</creatorcontrib><title>Induction of a Specific Humoral Immune Response by Nasal Delivery of Bcla2ctd of Clostridioides difficile</title><title>International journal of molecular sciences</title><description>Clostridioides difficile, formerly known as Clostridium difficile, is a spore-forming bacterium considered as the most common cause of nosocomial infections in developed countries. The spore of C. difficile is involved in the transmission of the pathogen and in its first interaction with the host; therefore, a therapeutic approach able to control C. difficile spores would improve the clearance of the infection. The C-terminal (CTD) end of BclA2, a spore surface protein of C. difficile responsible of the interaction with the host intestinal cells, was selected as a putative mucosal antigen. The BclA2 fragment, BclA2CTD, was purified and used to nasally immunize mice both as a free protein and after adsorption to the spore of Bacillus subtilis, a well-established mucosal delivery vehicle. While the adsorption to spores increased the in vitro stability of BclA2CTD, in vivo both free and spore-adsorbed BclA2CTD were able to induce a similar, specific humoral immune response in a murine model. Although in the experimental conditions utilized the immune response was not protective, the induction of specific IgG indicates that free or spore-bound BclA2CTD could act as a putative mucosal antigen targeting C. difficile spores.</description><issn>1422-0067</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNpVkVtLAzEQhYMoVqtv_oA8-mB1Npem-yJovRWKgpfnkE0mmrK7qZvdQv-9WyxSn-bAOfMNwyHkLINLznO4CosqsQxExpTaI0eZYGwEMFb7O3pAjlNaADDOZH5IBpyBErmURyTMatfZNsSaRk8NfVuiDT5Y-tRVsTElnVVVVyN9xbSMdUJarOmzSb1xh2VYYbPe7N3a0jDbuo2eljG1TXAhBoeJuuB7XCjxhBx4UyY83c4h-Xi4f58-jeYvj7PpzXxkmRqrEbeA4CdSSsG4cj4XgIUQJisA0DplQEghAAp0Xk3M2CvBxnmWKy_R59bxIbn-5S67okJnsW77P_SyCZVp1jqaoP87dfjSn3GlFSg2mbAecL4FNPG7w9TqKiSLZWlqjF3SjMtcZIpz6KMXv1HbxJQa9H9nMtCbdvRuO_wHLc6CrQ</recordid><startdate>20200214</startdate><enddate>20200214</enddate><creator>Maia, Ana Raquel</creator><creator>Reyes-Ramírez, Rodrigo</creator><creator>Pizarro-Guajardo, Marjorie</creator><creator>Saggese, Anella</creator><creator>Castro-Córdova, Pablo</creator><creator>Isticato, Rachele</creator><creator>Ricca, Ezio</creator><creator>Paredes-Sabja, Daniel</creator><creator>Baccigalupi, Loredana</creator><general>MDPI</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-3370-4878</orcidid><orcidid>https://orcid.org/0000-0002-6176-9943</orcidid></search><sort><creationdate>20200214</creationdate><title>Induction of a Specific Humoral Immune Response by Nasal Delivery of Bcla2ctd of Clostridioides difficile</title><author>Maia, Ana Raquel ; Reyes-Ramírez, Rodrigo ; Pizarro-Guajardo, Marjorie ; Saggese, Anella ; Castro-Córdova, Pablo ; Isticato, Rachele ; Ricca, Ezio ; Paredes-Sabja, Daniel ; Baccigalupi, Loredana</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2767-3c0e0f85554237df940eb44a1b00ecd7a0454400bedf78a6f74269197f5ef9cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maia, Ana Raquel</creatorcontrib><creatorcontrib>Reyes-Ramírez, Rodrigo</creatorcontrib><creatorcontrib>Pizarro-Guajardo, Marjorie</creatorcontrib><creatorcontrib>Saggese, Anella</creatorcontrib><creatorcontrib>Castro-Córdova, Pablo</creatorcontrib><creatorcontrib>Isticato, Rachele</creatorcontrib><creatorcontrib>Ricca, Ezio</creatorcontrib><creatorcontrib>Paredes-Sabja, Daniel</creatorcontrib><creatorcontrib>Baccigalupi, Loredana</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maia, Ana Raquel</au><au>Reyes-Ramírez, Rodrigo</au><au>Pizarro-Guajardo, Marjorie</au><au>Saggese, Anella</au><au>Castro-Córdova, Pablo</au><au>Isticato, Rachele</au><au>Ricca, Ezio</au><au>Paredes-Sabja, Daniel</au><au>Baccigalupi, Loredana</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of a Specific Humoral Immune Response by Nasal Delivery of Bcla2ctd of Clostridioides difficile</atitle><jtitle>International journal of molecular sciences</jtitle><date>2020-02-14</date><risdate>2020</risdate><volume>21</volume><issue>4</issue><spage>1277</spage><pages>1277-</pages><issn>1422-0067</issn><eissn>1422-0067</eissn><abstract>Clostridioides difficile, formerly known as Clostridium difficile, is a spore-forming bacterium considered as the most common cause of nosocomial infections in developed countries. The spore of C. difficile is involved in the transmission of the pathogen and in its first interaction with the host; therefore, a therapeutic approach able to control C. difficile spores would improve the clearance of the infection. The C-terminal (CTD) end of BclA2, a spore surface protein of C. difficile responsible of the interaction with the host intestinal cells, was selected as a putative mucosal antigen. The BclA2 fragment, BclA2CTD, was purified and used to nasally immunize mice both as a free protein and after adsorption to the spore of Bacillus subtilis, a well-established mucosal delivery vehicle. While the adsorption to spores increased the in vitro stability of BclA2CTD, in vivo both free and spore-adsorbed BclA2CTD were able to induce a similar, specific humoral immune response in a murine model. Although in the experimental conditions utilized the immune response was not protective, the induction of specific IgG indicates that free or spore-bound BclA2CTD could act as a putative mucosal antigen targeting C. difficile spores.</abstract><pub>MDPI</pub><pmid>32074955</pmid><doi>10.3390/ijms21041277</doi><orcidid>https://orcid.org/0000-0002-3370-4878</orcidid><orcidid>https://orcid.org/0000-0002-6176-9943</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1422-0067
ispartof International journal of molecular sciences, 2020-02, Vol.21 (4), p.1277
issn 1422-0067
1422-0067
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7072882
source MDPI - Multidisciplinary Digital Publishing Institute; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central
title Induction of a Specific Humoral Immune Response by Nasal Delivery of Bcla2ctd of Clostridioides difficile
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T18%3A50%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Induction%20of%20a%20Specific%20Humoral%20Immune%20Response%20by%20Nasal%20Delivery%20of%20Bcla2ctd%20of%20Clostridioides%20difficile&rft.jtitle=International%20journal%20of%20molecular%20sciences&rft.au=Maia,%20Ana%20Raquel&rft.date=2020-02-14&rft.volume=21&rft.issue=4&rft.spage=1277&rft.pages=1277-&rft.issn=1422-0067&rft.eissn=1422-0067&rft_id=info:doi/10.3390/ijms21041277&rft_dat=%3Cproquest_pubme%3E2359417330%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2359417330&rft_id=info:pmid/32074955&rfr_iscdi=true