Re-evaluation of human BDCA-2+ DC during acute sterile skin inflammation
Plasmacytoid dendritic cells (pDCs) produce type I interferon (IFN-I) and are traditionally defined as being BDCA-2+CD123+. pDCs are not readily detectable in healthy human skin, but have been suggested to accumulate in wounds. Here, we describe a CD1a-bearing BDCA-2+CD123int DC subset that rapidly...
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| Veröffentlicht in: | The Journal of experimental medicine 2020-03, Vol.217 (3) |
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| creator | Chen, Yi-Ling Gomes, Tomas Hardman, Clare S Vieira Braga, Felipe A Gutowska-Owsiak, Danuta Salimi, Maryam Gray, Nicki Duncan, David A Reynolds, Gary Johnson, David Salio, Mariolina Cerundolo, Vincenzo Barlow, Jillian L McKenzie, Andrew N J Teichmann, Sarah A Haniffa, Muzlifah Ogg, Graham |
| description | Plasmacytoid dendritic cells (pDCs) produce type I interferon (IFN-I) and are traditionally defined as being BDCA-2+CD123+. pDCs are not readily detectable in healthy human skin, but have been suggested to accumulate in wounds. Here, we describe a CD1a-bearing BDCA-2+CD123int DC subset that rapidly infiltrates human skin wounds and comprises a major DC population. Using single-cell RNA sequencing, we show that these cells are largely activated DCs acquiring features compatible with lymph node homing and antigen presentation, but unexpectedly express both BDCA-2 and CD123, potentially mimicking pDCs. Furthermore, a third BDCA-2-expressing population, Axl+Siglec-6+ DCs (ASDC), was also found to infiltrate human skin during wounding. These data demonstrate early skin infiltration of a previously unrecognized CD123intBDCA-2+CD1a+ DC subset during acute sterile inflammation, and prompt a re-evaluation of previously ascribed pDC involvement in skin disease. |
| doi_str_mv | 10.1084/jem.20190811 |
| format | Article |
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Here, we describe a CD1a-bearing BDCA-2+CD123int DC subset that rapidly infiltrates human skin wounds and comprises a major DC population. Using single-cell RNA sequencing, we show that these cells are largely activated DCs acquiring features compatible with lymph node homing and antigen presentation, but unexpectedly express both BDCA-2 and CD123, potentially mimicking pDCs. Furthermore, a third BDCA-2-expressing population, Axl+Siglec-6+ DCs (ASDC), was also found to infiltrate human skin during wounding. These data demonstrate early skin infiltration of a previously unrecognized CD123intBDCA-2+CD1a+ DC subset during acute sterile inflammation, and prompt a re-evaluation of previously ascribed pDC involvement in skin disease.</description><identifier>ISSN: 0022-1007</identifier><identifier>EISSN: 1540-9538</identifier><identifier>DOI: 10.1084/jem.20190811</identifier><identifier>PMID: 31845972</identifier><language>eng</language><publisher>United States: Rockefeller University Press</publisher><subject>Antigen Presentation - physiology ; Antigens, CD1 - metabolism ; Dendritic Cells - metabolism ; Humans ; Inflammation - metabolism ; Interleukin-3 Receptor alpha Subunit - metabolism ; Lectins, C-Type - metabolism ; Lymph Nodes - metabolism ; Membrane Glycoproteins - metabolism ; Receptors, Immunologic - metabolism ; Skin - metabolism</subject><ispartof>The Journal of experimental medicine, 2020-03, Vol.217 (3)</ispartof><rights>2019 Chen et al.</rights><rights>2019 Chen et al. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-847bc7eebcc943d68562a0bd97aab5b89b81d585927516fffdc54b79bfd16ce13</citedby><cites>FETCH-LOGICAL-c423t-847bc7eebcc943d68562a0bd97aab5b89b81d585927516fffdc54b79bfd16ce13</cites><orcidid>0000-0003-3758-0638 ; 0000-0003-0040-3793 ; 0000-0003-4503-2279 ; 0000-0002-0827-2022 ; 0000-0002-1127-6446 ; 0000-0001-9757-2512 ; 0000-0003-1333-0191 ; 0000-0002-3097-045X ; 0000-0002-4821-3801 ; 0000-0001-9149-0831</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31845972$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Yi-Ling</creatorcontrib><creatorcontrib>Gomes, Tomas</creatorcontrib><creatorcontrib>Hardman, Clare S</creatorcontrib><creatorcontrib>Vieira Braga, Felipe A</creatorcontrib><creatorcontrib>Gutowska-Owsiak, Danuta</creatorcontrib><creatorcontrib>Salimi, Maryam</creatorcontrib><creatorcontrib>Gray, Nicki</creatorcontrib><creatorcontrib>Duncan, David A</creatorcontrib><creatorcontrib>Reynolds, Gary</creatorcontrib><creatorcontrib>Johnson, David</creatorcontrib><creatorcontrib>Salio, Mariolina</creatorcontrib><creatorcontrib>Cerundolo, Vincenzo</creatorcontrib><creatorcontrib>Barlow, Jillian L</creatorcontrib><creatorcontrib>McKenzie, Andrew N J</creatorcontrib><creatorcontrib>Teichmann, Sarah A</creatorcontrib><creatorcontrib>Haniffa, Muzlifah</creatorcontrib><creatorcontrib>Ogg, Graham</creatorcontrib><title>Re-evaluation of human BDCA-2+ DC during acute sterile skin inflammation</title><title>The Journal of experimental medicine</title><addtitle>J Exp Med</addtitle><description>Plasmacytoid dendritic cells (pDCs) produce type I interferon (IFN-I) and are traditionally defined as being BDCA-2+CD123+. pDCs are not readily detectable in healthy human skin, but have been suggested to accumulate in wounds. 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These data demonstrate early skin infiltration of a previously unrecognized CD123intBDCA-2+CD1a+ DC subset during acute sterile inflammation, and prompt a re-evaluation of previously ascribed pDC involvement in skin disease.</description><subject>Antigen Presentation - physiology</subject><subject>Antigens, CD1 - metabolism</subject><subject>Dendritic Cells - metabolism</subject><subject>Humans</subject><subject>Inflammation - metabolism</subject><subject>Interleukin-3 Receptor alpha Subunit - metabolism</subject><subject>Lectins, C-Type - metabolism</subject><subject>Lymph Nodes - metabolism</subject><subject>Membrane Glycoproteins - metabolism</subject><subject>Receptors, Immunologic - metabolism</subject><subject>Skin - metabolism</subject><issn>0022-1007</issn><issn>1540-9538</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkM9LwzAYhoMobk5vnqVHQatJmjTJRZidOmEgiJ5DkqZbZpvOph3431vdD_T0Hr7ne7-PB4BzBG8Q5OR2aasbDJGAHKEDMESUwFjQhB-CIYQYxwhCNgAnISwhRITQ9BgMEsQJFQwPwfTVxnatyk61rvZRXUSLrlI-up9k4xhfRZMsyrvG-XmkTNfaKLS2cWWfH85Hzhelqqrf1VNwVKgy2LNtjsD748NbNo1nL0_P2XgWG4KTNuaEacOs1cYIkuQppylWUOeCKaWp5kJzlFNOBWYUpUVR5IYSzYQucpQai5IRuNv0rjpd2dxY3zaqlKvGVar5krVy8v_Eu4Wc12vJYIoppn3B5bagqT87G1pZuWBsWSpv6y5InGAmEpRS0aPXG9Q0dQiNLfZnEJQ_8mUvX-7k9_jF39f28M528g1csYBR</recordid><startdate>20200302</startdate><enddate>20200302</enddate><creator>Chen, Yi-Ling</creator><creator>Gomes, Tomas</creator><creator>Hardman, Clare S</creator><creator>Vieira Braga, Felipe A</creator><creator>Gutowska-Owsiak, Danuta</creator><creator>Salimi, Maryam</creator><creator>Gray, Nicki</creator><creator>Duncan, David A</creator><creator>Reynolds, Gary</creator><creator>Johnson, David</creator><creator>Salio, Mariolina</creator><creator>Cerundolo, Vincenzo</creator><creator>Barlow, Jillian L</creator><creator>McKenzie, Andrew N J</creator><creator>Teichmann, Sarah A</creator><creator>Haniffa, Muzlifah</creator><creator>Ogg, Graham</creator><general>Rockefeller University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-3758-0638</orcidid><orcidid>https://orcid.org/0000-0003-0040-3793</orcidid><orcidid>https://orcid.org/0000-0003-4503-2279</orcidid><orcidid>https://orcid.org/0000-0002-0827-2022</orcidid><orcidid>https://orcid.org/0000-0002-1127-6446</orcidid><orcidid>https://orcid.org/0000-0001-9757-2512</orcidid><orcidid>https://orcid.org/0000-0003-1333-0191</orcidid><orcidid>https://orcid.org/0000-0002-3097-045X</orcidid><orcidid>https://orcid.org/0000-0002-4821-3801</orcidid><orcidid>https://orcid.org/0000-0001-9149-0831</orcidid></search><sort><creationdate>20200302</creationdate><title>Re-evaluation of human BDCA-2+ DC during acute sterile skin inflammation</title><author>Chen, Yi-Ling ; 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| subjects | Antigen Presentation - physiology Antigens, CD1 - metabolism Dendritic Cells - metabolism Humans Inflammation - metabolism Interleukin-3 Receptor alpha Subunit - metabolism Lectins, C-Type - metabolism Lymph Nodes - metabolism Membrane Glycoproteins - metabolism Receptors, Immunologic - metabolism Skin - metabolism |
| title | Re-evaluation of human BDCA-2+ DC during acute sterile skin inflammation |
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