A novel workflow for three-dimensional analysis of tumour cell migration

A novel workflow for three-dimensional analysis of tumour cell migration

The limitations of two-dimensional analysis in three-dimensional (3D) cellular imaging impair the accuracy of research findings in biological studies. Here, we report a novel 3D approach to acquisition, analysis and interpretation of tumour spheroid images. Our research interest in mesenchymal-amoeb...

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Veröffentlicht in:Interface focus 2020-04, Vol.10 (2), p.20190070-20190070, Article 20190070
Hauptverfasser: Ketchen, Sophie, Rohwedder, Arndt, Knipp, Sabine, Esteves, Filomena, Struve, Nina, Peckham, Michelle, Ladbury, John E., Curd, Alistair, Short, Susan C., Bruning-Richardson, Anke
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Life Sciences & Biomedicine - Other Topics
Science & Technology
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container_end_page 20190070
container_issue 2
container_start_page 20190070
container_title Interface focus
container_volume 10
creator Ketchen, Sophie
Rohwedder, Arndt
Knipp, Sabine
Esteves, Filomena
Struve, Nina
Peckham, Michelle
Ladbury, John E.
Curd, Alistair
Short, Susan C.
Bruning-Richardson, Anke
description The limitations of two-dimensional analysis in three-dimensional (3D) cellular imaging impair the accuracy of research findings in biological studies. Here, we report a novel 3D approach to acquisition, analysis and interpretation of tumour spheroid images. Our research interest in mesenchymal-amoeboid transition led to the development of a workflow incorporating the generation and analysis of 3D data with instant structured illumination microscopy and a new ImageJ plugin.
doi_str_mv 10.1098/rsfs.2019.0070
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subjects Biology
Life Sciences & Biomedicine
Life Sciences & Biomedicine - Other Topics
Science & Technology
title A novel workflow for three-dimensional analysis of tumour cell migration
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