Implementation of a Multiplex rRT-PCR for Zika, Chikungunya, and Dengue Viruses: Improving Arboviral Detection in an Endemic Region
Arboviral diagnosis has been complicated throughout the tropical and subtropical Americas by the recent co-circulation of Zika virus (ZIKV), chikungunya virus (CHIKV), and dengue virus (DENV). The aim of this study was to implement a multiplex real-time RT-PCR (rRT-PCR) for ZIKV, CHIKV, and DENV in...
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| Veröffentlicht in: | The American journal of tropical medicine and hygiene 2020-03, Vol.102 (3), p.625-628 |
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| creator | Cantero, César Cardozo, Fátima Waggoner, Jesse J Pinsky, Benjamin A Espínola, Anibal Infanzón, Belén Acosta, María Eugenia Aria, Laura Arévalo de Guillén, Yvalena Cuevas, Teresa Rojas, Vicenta Segovia, Clotilde Centurión, Ana Rojas, Alejandra |
| description | Arboviral diagnosis has been complicated throughout the tropical and subtropical Americas by the recent co-circulation of Zika virus (ZIKV), chikungunya virus (CHIKV), and dengue virus (DENV). The aim of this study was to implement a multiplex real-time RT-PCR (rRT-PCR) for ZIKV, CHIKV, and DENV in Paraguay to test patients who were clinically suspected of having dengue. We tested 110 sera from patients who presented to the Hospital de Clínicas in 2016 and had testing for DENV nonstructural protein 1 (NS1; 40 positive and 70 negative). Using a composite reference standard, we confirmed 51 dengue cases (46.4%): 38/40 NS1 positive and 13/70 NS1 negative. Chikungunya virus and ZIKV were detected in one sample each, both were DENV NS1 negative. The NS1 test demonstrated good agreement with rRT-PCR for DENV. However, multiplex rRT-PCR identified a subset of dengue cases and additional arboviral infections that would not be detected if NS1 assays are relied upon for diagnosis. |
| doi_str_mv | 10.4269/ajtmh.19-0707 |
| format | Article |
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However, multiplex rRT-PCR identified a subset of dengue cases and additional arboviral infections that would not be detected if NS1 assays are relied upon for diagnosis.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Chikungunya Fever - diagnosis</subject><subject>Chikungunya Fever - epidemiology</subject><subject>Chikungunya virus</subject><subject>Child</subject><subject>Dengue - diagnosis</subject><subject>Dengue - epidemiology</subject><subject>Dengue fever</subject><subject>Endemic Diseases</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Multiplex Polymerase Chain Reaction - methods</subject><subject>Paraguay - epidemiology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - methods</subject><subject>Young Adult</subject><subject>Zika virus</subject><subject>Zika Virus Infection - diagnosis</subject><subject>Zika Virus Infection - epidemiology</subject><issn>0002-9637</issn><issn>1476-1645</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc1rFTEUxYMo9rW6dCsBNy6cmo-ZJOOiUJ5VCxXlUV24CUkm815eZ5JnMlPatf-4tx8WFQLJvfndwz0chF5Qclgz0b4122ncHNK2IpLIR2hBaykqKurmMVoQQljVCi730H4pW0KoYpQ-RXuctpzXgi3Qr9NxN_jRx8lMIUWcemzw53mYArSvcF6dV1-XK9ynjH-EC_MGLzfhYo7rOV5DYWKH33uoPP4e8lx8eYdBMKfLENf4OFt4ZDMAM3l3qx8iDOGT2PkxOLzya2g-Q096MxT__P4-QN8-nJwvP1VnXz6eLo_PKlfTZqp4J62yVna9E6qjom-4cqyz3sLpa9pZ5TtXt84rwZWyYNIxLq21jjW8Y_wAHd3p7mY7AgqmYTm9y2E0-VonE_S_PzFs9DpdakkaUXMOAq_vBXL6Ofsy6TEU54fBRJ_mohnnirQSIgD01X_oNs05gj2gZKMYEaIBqrqjXE6lZN8_LEOJvolX38araatv4gX-5d8OHug_efLfN6ekFA</recordid><startdate>20200301</startdate><enddate>20200301</enddate><creator>Cantero, César</creator><creator>Cardozo, Fátima</creator><creator>Waggoner, Jesse J</creator><creator>Pinsky, Benjamin A</creator><creator>Espínola, Anibal</creator><creator>Infanzón, Belén</creator><creator>Acosta, María Eugenia</creator><creator>Aria, Laura</creator><creator>Arévalo de Guillén, Yvalena</creator><creator>Cuevas, Teresa</creator><creator>Rojas, Vicenta</creator><creator>Segovia, Clotilde</creator><creator>Centurión, Ana</creator><creator>Rojas, Alejandra</creator><general>Institute of Tropical Medicine</general><general>The American Society of Tropical Medicine and Hygiene</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20200301</creationdate><title>Implementation of a Multiplex rRT-PCR for Zika, Chikungunya, and Dengue Viruses: Improving Arboviral Detection in an Endemic Region</title><author>Cantero, César ; Cardozo, Fátima ; Waggoner, Jesse J ; Pinsky, Benjamin A ; Espínola, Anibal ; Infanzón, Belén ; Acosta, María Eugenia ; Aria, Laura ; Arévalo de Guillén, Yvalena ; Cuevas, Teresa ; Rojas, Vicenta ; Segovia, Clotilde ; Centurión, Ana ; Rojas, Alejandra</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-3d7b8bb7dfc68d16f538c2dbebbebf41db8edc49ce86388b319c237bbbc253d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Chikungunya Fever - diagnosis</topic><topic>Chikungunya Fever - epidemiology</topic><topic>Chikungunya virus</topic><topic>Child</topic><topic>Dengue - diagnosis</topic><topic>Dengue - epidemiology</topic><topic>Dengue fever</topic><topic>Endemic Diseases</topic><topic>Female</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Multiplex Polymerase Chain Reaction - methods</topic><topic>Paraguay - epidemiology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - methods</topic><topic>Young Adult</topic><topic>Zika virus</topic><topic>Zika Virus Infection - diagnosis</topic><topic>Zika Virus Infection - epidemiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cantero, César</creatorcontrib><creatorcontrib>Cardozo, Fátima</creatorcontrib><creatorcontrib>Waggoner, Jesse J</creatorcontrib><creatorcontrib>Pinsky, Benjamin A</creatorcontrib><creatorcontrib>Espínola, Anibal</creatorcontrib><creatorcontrib>Infanzón, Belén</creatorcontrib><creatorcontrib>Acosta, María Eugenia</creatorcontrib><creatorcontrib>Aria, Laura</creatorcontrib><creatorcontrib>Arévalo de Guillén, Yvalena</creatorcontrib><creatorcontrib>Cuevas, Teresa</creatorcontrib><creatorcontrib>Rojas, Vicenta</creatorcontrib><creatorcontrib>Segovia, Clotilde</creatorcontrib><creatorcontrib>Centurión, Ana</creatorcontrib><creatorcontrib>Rojas, Alejandra</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The American journal of tropical medicine and hygiene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cantero, César</au><au>Cardozo, Fátima</au><au>Waggoner, Jesse J</au><au>Pinsky, Benjamin A</au><au>Espínola, Anibal</au><au>Infanzón, Belén</au><au>Acosta, María Eugenia</au><au>Aria, Laura</au><au>Arévalo de Guillén, Yvalena</au><au>Cuevas, Teresa</au><au>Rojas, Vicenta</au><au>Segovia, Clotilde</au><au>Centurión, Ana</au><au>Rojas, Alejandra</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Implementation of a Multiplex rRT-PCR for Zika, Chikungunya, and Dengue Viruses: Improving Arboviral Detection in an Endemic Region</atitle><jtitle>The American journal of tropical medicine and hygiene</jtitle><addtitle>Am J Trop Med Hyg</addtitle><date>2020-03-01</date><risdate>2020</risdate><volume>102</volume><issue>3</issue><spage>625</spage><epage>628</epage><pages>625-628</pages><issn>0002-9637</issn><eissn>1476-1645</eissn><abstract>Arboviral diagnosis has been complicated throughout the tropical and subtropical Americas by the recent co-circulation of Zika virus (ZIKV), chikungunya virus (CHIKV), and dengue virus (DENV). 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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Adolescent Adult Chikungunya Fever - diagnosis Chikungunya Fever - epidemiology Chikungunya virus Child Dengue - diagnosis Dengue - epidemiology Dengue fever Endemic Diseases Female Humans Male Middle Aged Multiplex Polymerase Chain Reaction - methods Paraguay - epidemiology Reverse Transcriptase Polymerase Chain Reaction - methods Young Adult Zika virus Zika Virus Infection - diagnosis Zika Virus Infection - epidemiology |
| title | Implementation of a Multiplex rRT-PCR for Zika, Chikungunya, and Dengue Viruses: Improving Arboviral Detection in an Endemic Region |
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