Circular RNA HIPK3 downregulation mediates hydrogen peroxide-induced cytotoxicity in human osteoblasts
Hydrogen peroxide (H O ) induces oxidative injury to human osteoblasts. The expression and potential function of circular RNA HIPK3 (circHIPK3) in H O -treated human osteoblasts were tested. We show that H O significantly downregulated circHIPK3 in OB-6 cells and primary human osteoblasts. Furthermo...
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| Veröffentlicht in: | Aging (Albany, NY.) NY.), 2020-01, Vol.12 (2), p.1159-1170 |
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| creator | Liang, Jinqian Shen, Ying-Chao Zhang, Xiang-Yang Chen, Chong Zhao, Hong Hu, Jianhua |
| description | Hydrogen peroxide (H
O
) induces oxidative injury to human osteoblasts. The expression and potential function of circular RNA HIPK3 (circHIPK3) in H
O
-treated human osteoblasts were tested. We show that H
O
significantly downregulated circHIPK3 in OB-6 cells and primary human osteoblasts. Furthermore, circHIPK3 levels were decreased in the necrotic femoral head tissues of dexamethasone-treated patients. In OB-6 osteoblastic cells and primary human osteoblasts, forced overexpression of circHIPK3 by a lentiviral construct alleviated H
O
-induced viability reduction, cell death and apoptosis. Contrarily, circHIPK3 silencing by targeted shRNA potentiated H
O
-induced cytotoxicity in OB-6 cells and primary human osteoblasts. Moreover, circHIPK3 downregulation by H
O
induced miR-124 accumulation in OB-6 cells and primary human osteoblasts. On the contrary, miR-124 inhibition by transfection of the miR-124 inhibitor protected human osteoblasts from H
O
. Importantly, forced overexpression of miR-124 by transfection of the miR-124 mimic induced significant cytotoxicity in OB-6 cells and primary human osteoblasts. H
O
downregulated miR-124's targets, cyclin dependent kinase 6 and Rho-Associated Protein Kinase 1, in human osteoblasts. In conclusion circHIPK3 downregulation mediates H
O
-induced cytotoxicity in human osteoblasts. |
| doi_str_mv | 10.18632/aging.102674 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7053588</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>31955154</sourcerecordid><originalsourceid>FETCH-LOGICAL-c387t-b01e3db9a0e68aa42476639d22dc27af71883e6fc34caf68af86db1cfec1351b3</originalsourceid><addsrcrecordid>eNpVkMtOwzAQRS0EoqWwZIv8Aylx_EiyQaoqoBUVIATryPEjNUrtynaA_D1RC6isZnTnzp3RAeASpVNUMJxd88bYZorSjOXkCIxRSWhCaFEeH_QjcBbCe5oySgk7BSOMSkoRJWOg58aLruUevjzO4GL5_IChdJ_Wq2ZQo3EWbpQ0PKoA1730rlEWbpV3X0aqxFjZCSWh6KOLgyRM7KGxcN1tuIUuROXqlocYzsGJ5m1QFz91At7ubl_ni2T1dL-cz1aJwEUekzpFCsu65KliBeckIzljuJRZJkWWc52josCKaYGJ4Hqw6ILJGgmtBMIU1XgCbva5264e_hbKRs_bauvNhvu-ctxU_yfWrKvGfVR5SjEdwicg2QcI70LwSv_torTaAa92wKs98MF_dXjwz_1LGH8DzuOBEQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Circular RNA HIPK3 downregulation mediates hydrogen peroxide-induced cytotoxicity in human osteoblasts</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central Open Access</source><source>PubMed Central</source><creator>Liang, Jinqian ; Shen, Ying-Chao ; Zhang, Xiang-Yang ; Chen, Chong ; Zhao, Hong ; Hu, Jianhua</creator><creatorcontrib>Liang, Jinqian ; Shen, Ying-Chao ; Zhang, Xiang-Yang ; Chen, Chong ; Zhao, Hong ; Hu, Jianhua</creatorcontrib><description>Hydrogen peroxide (H
O
) induces oxidative injury to human osteoblasts. The expression and potential function of circular RNA HIPK3 (circHIPK3) in H
O
-treated human osteoblasts were tested. We show that H
O
significantly downregulated circHIPK3 in OB-6 cells and primary human osteoblasts. Furthermore, circHIPK3 levels were decreased in the necrotic femoral head tissues of dexamethasone-treated patients. In OB-6 osteoblastic cells and primary human osteoblasts, forced overexpression of circHIPK3 by a lentiviral construct alleviated H
O
-induced viability reduction, cell death and apoptosis. Contrarily, circHIPK3 silencing by targeted shRNA potentiated H
O
-induced cytotoxicity in OB-6 cells and primary human osteoblasts. Moreover, circHIPK3 downregulation by H
O
induced miR-124 accumulation in OB-6 cells and primary human osteoblasts. On the contrary, miR-124 inhibition by transfection of the miR-124 inhibitor protected human osteoblasts from H
O
. Importantly, forced overexpression of miR-124 by transfection of the miR-124 mimic induced significant cytotoxicity in OB-6 cells and primary human osteoblasts. H
O
downregulated miR-124's targets, cyclin dependent kinase 6 and Rho-Associated Protein Kinase 1, in human osteoblasts. In conclusion circHIPK3 downregulation mediates H
O
-induced cytotoxicity in human osteoblasts.</description><identifier>ISSN: 1945-4589</identifier><identifier>EISSN: 1945-4589</identifier><identifier>DOI: 10.18632/aging.102674</identifier><identifier>PMID: 31955154</identifier><language>eng</language><publisher>United States: Impact Journals</publisher><subject>Apoptosis - drug effects ; Apoptosis - genetics ; Cell Death - drug effects ; Cell Death - genetics ; Cell Survival - drug effects ; Cell Survival - genetics ; Cells, Cultured ; Ectopic Gene Expression ; Gene Expression Regulation - drug effects ; Humans ; Hydrogen Peroxide - metabolism ; Hydrogen Peroxide - pharmacology ; Intracellular Signaling Peptides and Proteins - genetics ; Osteoblasts - drug effects ; Osteoblasts - metabolism ; Protein-Serine-Threonine Kinases - genetics ; Research Paper ; RNA Interference ; RNA, Circular ; RNA, Small Interfering - genetics</subject><ispartof>Aging (Albany, NY.), 2020-01, Vol.12 (2), p.1159-1170</ispartof><rights>Copyright © 2020 Liang et al.</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c387t-b01e3db9a0e68aa42476639d22dc27af71883e6fc34caf68af86db1cfec1351b3</citedby><cites>FETCH-LOGICAL-c387t-b01e3db9a0e68aa42476639d22dc27af71883e6fc34caf68af86db1cfec1351b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053588/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053588/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31955154$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liang, Jinqian</creatorcontrib><creatorcontrib>Shen, Ying-Chao</creatorcontrib><creatorcontrib>Zhang, Xiang-Yang</creatorcontrib><creatorcontrib>Chen, Chong</creatorcontrib><creatorcontrib>Zhao, Hong</creatorcontrib><creatorcontrib>Hu, Jianhua</creatorcontrib><title>Circular RNA HIPK3 downregulation mediates hydrogen peroxide-induced cytotoxicity in human osteoblasts</title><title>Aging (Albany, NY.)</title><addtitle>Aging (Albany NY)</addtitle><description>Hydrogen peroxide (H
O
) induces oxidative injury to human osteoblasts. The expression and potential function of circular RNA HIPK3 (circHIPK3) in H
O
-treated human osteoblasts were tested. We show that H
O
significantly downregulated circHIPK3 in OB-6 cells and primary human osteoblasts. Furthermore, circHIPK3 levels were decreased in the necrotic femoral head tissues of dexamethasone-treated patients. In OB-6 osteoblastic cells and primary human osteoblasts, forced overexpression of circHIPK3 by a lentiviral construct alleviated H
O
-induced viability reduction, cell death and apoptosis. Contrarily, circHIPK3 silencing by targeted shRNA potentiated H
O
-induced cytotoxicity in OB-6 cells and primary human osteoblasts. Moreover, circHIPK3 downregulation by H
O
induced miR-124 accumulation in OB-6 cells and primary human osteoblasts. On the contrary, miR-124 inhibition by transfection of the miR-124 inhibitor protected human osteoblasts from H
O
. Importantly, forced overexpression of miR-124 by transfection of the miR-124 mimic induced significant cytotoxicity in OB-6 cells and primary human osteoblasts. H
O
downregulated miR-124's targets, cyclin dependent kinase 6 and Rho-Associated Protein Kinase 1, in human osteoblasts. In conclusion circHIPK3 downregulation mediates H
O
-induced cytotoxicity in human osteoblasts.</description><subject>Apoptosis - drug effects</subject><subject>Apoptosis - genetics</subject><subject>Cell Death - drug effects</subject><subject>Cell Death - genetics</subject><subject>Cell Survival - drug effects</subject><subject>Cell Survival - genetics</subject><subject>Cells, Cultured</subject><subject>Ectopic Gene Expression</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Humans</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>Hydrogen Peroxide - pharmacology</subject><subject>Intracellular Signaling Peptides and Proteins - genetics</subject><subject>Osteoblasts - drug effects</subject><subject>Osteoblasts - metabolism</subject><subject>Protein-Serine-Threonine Kinases - genetics</subject><subject>Research Paper</subject><subject>RNA Interference</subject><subject>RNA, Circular</subject><subject>RNA, Small Interfering - genetics</subject><issn>1945-4589</issn><issn>1945-4589</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkMtOwzAQRS0EoqWwZIv8Aylx_EiyQaoqoBUVIATryPEjNUrtynaA_D1RC6isZnTnzp3RAeASpVNUMJxd88bYZorSjOXkCIxRSWhCaFEeH_QjcBbCe5oySgk7BSOMSkoRJWOg58aLruUevjzO4GL5_IChdJ_Wq2ZQo3EWbpQ0PKoA1730rlEWbpV3X0aqxFjZCSWh6KOLgyRM7KGxcN1tuIUuROXqlocYzsGJ5m1QFz91At7ubl_ni2T1dL-cz1aJwEUekzpFCsu65KliBeckIzljuJRZJkWWc52josCKaYGJ4Hqw6ILJGgmtBMIU1XgCbva5264e_hbKRs_bauvNhvu-ctxU_yfWrKvGfVR5SjEdwicg2QcI70LwSv_torTaAa92wKs98MF_dXjwz_1LGH8DzuOBEQ</recordid><startdate>20200118</startdate><enddate>20200118</enddate><creator>Liang, Jinqian</creator><creator>Shen, Ying-Chao</creator><creator>Zhang, Xiang-Yang</creator><creator>Chen, Chong</creator><creator>Zhao, Hong</creator><creator>Hu, Jianhua</creator><general>Impact Journals</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20200118</creationdate><title>Circular RNA HIPK3 downregulation mediates hydrogen peroxide-induced cytotoxicity in human osteoblasts</title><author>Liang, Jinqian ; Shen, Ying-Chao ; Zhang, Xiang-Yang ; Chen, Chong ; Zhao, Hong ; Hu, Jianhua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c387t-b01e3db9a0e68aa42476639d22dc27af71883e6fc34caf68af86db1cfec1351b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Apoptosis - drug effects</topic><topic>Apoptosis - genetics</topic><topic>Cell Death - drug effects</topic><topic>Cell Death - genetics</topic><topic>Cell Survival - drug effects</topic><topic>Cell Survival - genetics</topic><topic>Cells, Cultured</topic><topic>Ectopic Gene Expression</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Humans</topic><topic>Hydrogen Peroxide - metabolism</topic><topic>Hydrogen Peroxide - pharmacology</topic><topic>Intracellular Signaling Peptides and Proteins - genetics</topic><topic>Osteoblasts - drug effects</topic><topic>Osteoblasts - metabolism</topic><topic>Protein-Serine-Threonine Kinases - genetics</topic><topic>Research Paper</topic><topic>RNA Interference</topic><topic>RNA, Circular</topic><topic>RNA, Small Interfering - genetics</topic><toplevel>online_resources</toplevel><creatorcontrib>Liang, Jinqian</creatorcontrib><creatorcontrib>Shen, Ying-Chao</creatorcontrib><creatorcontrib>Zhang, Xiang-Yang</creatorcontrib><creatorcontrib>Chen, Chong</creatorcontrib><creatorcontrib>Zhao, Hong</creatorcontrib><creatorcontrib>Hu, Jianhua</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Aging (Albany, NY.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liang, Jinqian</au><au>Shen, Ying-Chao</au><au>Zhang, Xiang-Yang</au><au>Chen, Chong</au><au>Zhao, Hong</au><au>Hu, Jianhua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Circular RNA HIPK3 downregulation mediates hydrogen peroxide-induced cytotoxicity in human osteoblasts</atitle><jtitle>Aging (Albany, NY.)</jtitle><addtitle>Aging (Albany NY)</addtitle><date>2020-01-18</date><risdate>2020</risdate><volume>12</volume><issue>2</issue><spage>1159</spage><epage>1170</epage><pages>1159-1170</pages><issn>1945-4589</issn><eissn>1945-4589</eissn><abstract>Hydrogen peroxide (H
O
) induces oxidative injury to human osteoblasts. The expression and potential function of circular RNA HIPK3 (circHIPK3) in H
O
-treated human osteoblasts were tested. We show that H
O
significantly downregulated circHIPK3 in OB-6 cells and primary human osteoblasts. Furthermore, circHIPK3 levels were decreased in the necrotic femoral head tissues of dexamethasone-treated patients. In OB-6 osteoblastic cells and primary human osteoblasts, forced overexpression of circHIPK3 by a lentiviral construct alleviated H
O
-induced viability reduction, cell death and apoptosis. Contrarily, circHIPK3 silencing by targeted shRNA potentiated H
O
-induced cytotoxicity in OB-6 cells and primary human osteoblasts. Moreover, circHIPK3 downregulation by H
O
induced miR-124 accumulation in OB-6 cells and primary human osteoblasts. On the contrary, miR-124 inhibition by transfection of the miR-124 inhibitor protected human osteoblasts from H
O
. Importantly, forced overexpression of miR-124 by transfection of the miR-124 mimic induced significant cytotoxicity in OB-6 cells and primary human osteoblasts. H
O
downregulated miR-124's targets, cyclin dependent kinase 6 and Rho-Associated Protein Kinase 1, in human osteoblasts. In conclusion circHIPK3 downregulation mediates H
O
-induced cytotoxicity in human osteoblasts.</abstract><cop>United States</cop><pub>Impact Journals</pub><pmid>31955154</pmid><doi>10.18632/aging.102674</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; PubMed Central |
| subjects | Apoptosis - drug effects Apoptosis - genetics Cell Death - drug effects Cell Death - genetics Cell Survival - drug effects Cell Survival - genetics Cells, Cultured Ectopic Gene Expression Gene Expression Regulation - drug effects Humans Hydrogen Peroxide - metabolism Hydrogen Peroxide - pharmacology Intracellular Signaling Peptides and Proteins - genetics Osteoblasts - drug effects Osteoblasts - metabolism Protein-Serine-Threonine Kinases - genetics Research Paper RNA Interference RNA, Circular RNA, Small Interfering - genetics |
| title | Circular RNA HIPK3 downregulation mediates hydrogen peroxide-induced cytotoxicity in human osteoblasts |
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