Two-dimensional random access multiphoton spatial frequency modulated imaging

Two-dimensional random access multiphoton spatial frequency modulated imaging

Spatial frequency modulated imaging (SPIFI) enables the use of an extended excitation source for linear and nonlinear imaging with single element detection. To date, SPIFI has only been used with fixed excitation source geometries. Here, we explore the potential for the SPIFI method when a spatial l...

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Veröffentlicht in:Optics express 2020-01, Vol.28 (1), p.405-424
Hauptverfasser: Allende Motz, Alyssa M, Czerski, John, Adams, Daniel E, Durfee, Charles, Bartels, Randy, Field, Jeff, Hoy, Christopher L, Squier, Jeff
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container_end_page 424
container_issue 1
container_start_page 405
container_title Optics express
container_volume 28
creator Allende Motz, Alyssa M
Czerski, John
Adams, Daniel E
Durfee, Charles
Bartels, Randy
Field, Jeff
Hoy, Christopher L
Squier, Jeff
description Spatial frequency modulated imaging (SPIFI) enables the use of an extended excitation source for linear and nonlinear imaging with single element detection. To date, SPIFI has only been used with fixed excitation source geometries. Here, we explore the potential for the SPIFI method when a spatial light modulator (SLM) is used to program the excitation source, opening the door to a more versatile, random access imaging environment. In addition, an in-line, quantitative pulse compensation and measurement scheme is demonstrated using a new technique, spectral phase and amplitude retrieval and compensation (SPARC). This enables full characterization of the light exposure conditions at the focal plane of the random access imaging system, an important metric for optimizing, and reporting imaging conditions within specimens.
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title Two-dimensional random access multiphoton spatial frequency modulated imaging
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